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Molecular identification of Prototheca zopfii genotype 2 mastitis isolates and their influence on the milk somatic cell count

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2017
484.pdf (574.4Kb)
Authors
Suvajdzić, Branko
Vasilev, Dragan
Karabasil, Nedeljko
Vučurović, Ivan
Čobanović, Nikola
Babić, Milijana
Katić, Vera
Article (Published version)
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Abstract
Algae from the genus Prototheca are the only plant-like microorganisms which can cause inflammation and alterations in the mammary gland. Prototheca mastitis is usually recognized as a chronic and symptomless disease with reduced milk production and a very high somatic cell count. Molecular identification of Prototheca spp. is helpful for the differentiation of pathogenic from non-pathogenic strains which are probably milk contaminants. Genotype-specific PCR assays, based on the 18S rDNA gene sequences, have recently been developed to differentiate three genotypes of Prototheca zopfii, of which Prototheca zopfii genotype 3 was reclassified in a new species: Prototheca blaschkeae. P. zopfii genotype 2 is characterized as the main causative agent of Prototheca mastitis that leads to significant economic losses in primary milk production. The purpose of this study was to give a molecular characterization of Prototheca strains isolated in cases of subclinical and clinical mastitis, as well... as to determine the influence of these pathogenic algae on the milk somatic cell count. After microbiological examination, algae from the genus Prototheca were isolated in pure cultures from 1.8% of all tested milk samples, and all 13 (100%) isolates were determined as Prototheca zopfii genotype 2 by a genotype-specific PCR. This study has provided the first molecular identification of Prototheca zopfii genotype 2 in the Republic of Serbia. In the case of subclinical Prototheca mastitis, the somatic cell count was 4,175,244 +/- 1,233,685/mL of milk. A distinctly higher somatic cell count (P lt 0.05) was found in the quarters infected by Prototheca zopfii genotype 2 than in the quarters infected by Staphylococcus aureus, which is the most common mastitis causative agent worldwide. The results from this study support previous observations that P. zopfii genotype 2 is the main causative agent of Prototheca mastitis which leads to a significant increase in the somatic cell count in the milk.

Keywords:
Prototheca mastitis / Prototheca zopfii genotype 2 / genotype-specific PCR / somatic cell count
Source:
Veterinarski Arhiv, 2017, 87, 3, 249-258
Publisher:
  • Univ Zagreb Vet Faculty, Zagreb
Projects:
  • Optimization of technological procedures and zootehnical resources on farms with the goal to upgrade sustainability of milk production (RS-31086)

DOI: 10.24099/vet.arhiv.151219

ISSN: 0372-5480

WoS: 000403577000002

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http://plantarum.izbis.bg.ac.rs/handle/123456789/486
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