Molecular Characterization of Pseudomonas syringae pv. coriandricola and Biochemical Changes Attributable to the Pathological Response on Its Hosts Carrot, Parsley, and Parsnip
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2019
Authors
Popović, TatjanaJelušić, Aleksandra
Dimkić, Ivica

Stanković, Slaviša

Poštić, Dobrivoj
Aleksić, Goran
Veljović Jovanović, Sonja
Article (Published version)

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Bacterial leaf spot caused by the plant pathogenic bacterium Pseudomonas syringae pv. coriandricola (Psc) was observed on carrot, parsnip, and parsley grown on a vegetable farm in the Vojvodina Province of Serbia. Nonfluorescent bacterial colonies were isolated from diseased leaves and characterized using different molecular techniques. Repetitive element PCR fingerprinting with five oligonucleotide primers (BOX, ERIC, GTG5, REP, and SERE) and the randomly amplified polymorphic DNA-PCR with the M13 primer revealed identical fingerprint patterns for all tested strains. Multilocus sequence analysis of four housekeeping genes (gapA, gltA, gyrB, and rpoD) showed a high degree (99.8 to 100%) of homology with sequences of Psc strains deposited in the Plant-Associated Microbes Database and NCBI database. The tested strains caused bacterial leaf spot symptoms on all three host plants. Host-strain specificity was not found in cross-pathogenicity tests, but the plant response (peroxidase inducti...on and chlorophyll bleaching) was more pronounced in carrot and parsley than in parsnip.
Keywords:
Pseudomonas syringae pv. coriandricola / carrot / parsley / parsnip / peroxidase induction / chlorophyll bleachingSource:
Plant Disease, 2019, 103, 12, 3072-3082Publisher:
- The American Phytopathological Society
Funding / projects:
- Molecular characterization of bacteria from genera Bacillus and Pseudomonas as potential agents for biological control (RS-173026)
- Modulation of antioxidative metabolism in plants for improvement of plant abiotic stress tolerance and identification of new biomarkers for application in remediation and monitoring of degraded biotopes (RS-43010)
DOI: 10.1094/PDIS-03-19-0674-RE
ISSN: 0191-2917
WoS: 000499765800011
Scopus: 2-s2.0-85075813149
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IZBISTY - JOUR AU - Popović, Tatjana AU - Jelušić, Aleksandra AU - Dimkić, Ivica AU - Stanković, Slaviša AU - Poštić, Dobrivoj AU - Aleksić, Goran AU - Veljović Jovanović, Sonja PY - 2019 UR - https://plantarum.izbis.bg.ac.rs/handle/123456789/587 AB - Bacterial leaf spot caused by the plant pathogenic bacterium Pseudomonas syringae pv. coriandricola (Psc) was observed on carrot, parsnip, and parsley grown on a vegetable farm in the Vojvodina Province of Serbia. Nonfluorescent bacterial colonies were isolated from diseased leaves and characterized using different molecular techniques. Repetitive element PCR fingerprinting with five oligonucleotide primers (BOX, ERIC, GTG5, REP, and SERE) and the randomly amplified polymorphic DNA-PCR with the M13 primer revealed identical fingerprint patterns for all tested strains. Multilocus sequence analysis of four housekeeping genes (gapA, gltA, gyrB, and rpoD) showed a high degree (99.8 to 100%) of homology with sequences of Psc strains deposited in the Plant-Associated Microbes Database and NCBI database. The tested strains caused bacterial leaf spot symptoms on all three host plants. Host-strain specificity was not found in cross-pathogenicity tests, but the plant response (peroxidase induction and chlorophyll bleaching) was more pronounced in carrot and parsley than in parsnip. PB - The American Phytopathological Society T2 - Plant Disease T1 - Molecular Characterization of Pseudomonas syringae pv. coriandricola and Biochemical Changes Attributable to the Pathological Response on Its Hosts Carrot, Parsley, and Parsnip EP - 3082 IS - 12 SP - 3072 VL - 103 DO - 10.1094/PDIS-03-19-0674-RE ER -
@article{ author = "Popović, Tatjana and Jelušić, Aleksandra and Dimkić, Ivica and Stanković, Slaviša and Poštić, Dobrivoj and Aleksić, Goran and Veljović Jovanović, Sonja", year = "2019", abstract = "Bacterial leaf spot caused by the plant pathogenic bacterium Pseudomonas syringae pv. coriandricola (Psc) was observed on carrot, parsnip, and parsley grown on a vegetable farm in the Vojvodina Province of Serbia. Nonfluorescent bacterial colonies were isolated from diseased leaves and characterized using different molecular techniques. Repetitive element PCR fingerprinting with five oligonucleotide primers (BOX, ERIC, GTG5, REP, and SERE) and the randomly amplified polymorphic DNA-PCR with the M13 primer revealed identical fingerprint patterns for all tested strains. Multilocus sequence analysis of four housekeeping genes (gapA, gltA, gyrB, and rpoD) showed a high degree (99.8 to 100%) of homology with sequences of Psc strains deposited in the Plant-Associated Microbes Database and NCBI database. The tested strains caused bacterial leaf spot symptoms on all three host plants. Host-strain specificity was not found in cross-pathogenicity tests, but the plant response (peroxidase induction and chlorophyll bleaching) was more pronounced in carrot and parsley than in parsnip.", publisher = "The American Phytopathological Society", journal = "Plant Disease", title = "Molecular Characterization of Pseudomonas syringae pv. coriandricola and Biochemical Changes Attributable to the Pathological Response on Its Hosts Carrot, Parsley, and Parsnip", pages = "3082-3072", number = "12", volume = "103", doi = "10.1094/PDIS-03-19-0674-RE" }
Popović, T., Jelušić, A., Dimkić, I., Stanković, S., Poštić, D., Aleksić, G.,& Veljović Jovanović, S.. (2019). Molecular Characterization of Pseudomonas syringae pv. coriandricola and Biochemical Changes Attributable to the Pathological Response on Its Hosts Carrot, Parsley, and Parsnip. in Plant Disease The American Phytopathological Society., 103(12), 3072-3082. https://doi.org/10.1094/PDIS-03-19-0674-RE
Popović T, Jelušić A, Dimkić I, Stanković S, Poštić D, Aleksić G, Veljović Jovanović S. Molecular Characterization of Pseudomonas syringae pv. coriandricola and Biochemical Changes Attributable to the Pathological Response on Its Hosts Carrot, Parsley, and Parsnip. in Plant Disease. 2019;103(12):3072-3082. doi:10.1094/PDIS-03-19-0674-RE .
Popović, Tatjana, Jelušić, Aleksandra, Dimkić, Ivica, Stanković, Slaviša, Poštić, Dobrivoj, Aleksić, Goran, Veljović Jovanović, Sonja, "Molecular Characterization of Pseudomonas syringae pv. coriandricola and Biochemical Changes Attributable to the Pathological Response on Its Hosts Carrot, Parsley, and Parsnip" in Plant Disease, 103, no. 12 (2019):3072-3082, https://doi.org/10.1094/PDIS-03-19-0674-RE . .