The undemanding LC-MS/MS method was developed for the synchronized analysis of atropine and scopolamine in maize crop. The dSPE was carried out with 1% acetic acid in acetonitrile/water and a mixture of magnesium sulphate, sodium chloride and sodium citrate. The analytes were separated on a Zorbax XDB C18 column using methanol/water as the mobile phase in gradient mode. The detection was done using a tandem masss spectrometry (MS/MS) in the positive ESI. The tropane alkaloids exhibited excellent linearity in the range of 2-20 µg/kg with the LOQ of 5 µg/kg for maize. The extraction recoveries of atropine and scopolamine were 65.7 and 85.5% with the intraday RSDr 10.25 and 4.29%, respectively. The validated method was appled to real maze samples. One sample contained 18.8 µg/kg of atropine and 6.3 µg/kg of scopolamine.
Source:
Savremena poljoprivreda, 2019, 67, 3-4, 221-226
Publisher:
Novi Sad : Univerzitet u Novom Sadu - Poljoprivredni fakultet
Novi Sad : Naučni institut za ratarstvo i povrtarstvo
TY - JOUR
AU - Vuković, Gorica
AU - Bursić, Vojislava
AU - Stojanović, Tijana
AU - Petrović, Aleksandra
AU - Gvozdenac, Sonja
AU - Starović, Mira
AU - Kuzmanović, Slobodan
AU - Aleksić, Goran
PY - 2019
UR - https://plantarum.izbis.bg.ac.rs/handle/123456789/609
AB - The undemanding LC-MS/MS method was developed for the synchronized analysis of atropine and scopolamine in maize crop. The dSPE was carried out with 1% acetic acid in acetonitrile/water and a mixture of magnesium sulphate, sodium chloride and sodium citrate. The analytes were separated on a Zorbax XDB C18 column using methanol/water as the mobile phase in gradient mode. The detection was done using a tandem masss spectrometry (MS/MS) in the positive ESI. The tropane alkaloids exhibited excellent linearity in the range of 2-20 µg/kg with the LOQ of 5 µg/kg for maize. The extraction recoveries of atropine and scopolamine were 65.7 and 85.5% with the intraday RSDr 10.25 and 4.29%, respectively. The validated method was appled to real maze samples. One sample contained 18.8 µg/kg of atropine and 6.3 µg/kg of scopolamine.
PB - Novi Sad : Univerzitet u Novom Sadu - Poljoprivredni fakultet
PB - Novi Sad : Naučni institut za ratarstvo i povrtarstvo
T2 - Savremena poljoprivreda
T1 - LC-MS/MS Determination of Tropane Alkaloids in Maize Crop
EP - 226
IS - 3-4
SP - 221
VL - 67
DO - 10.1515/contagri-2018-0032
DO - 10.1515/contagri-2018-0032
ER -
@article{
author = "Vuković, Gorica and Bursić, Vojislava and Stojanović, Tijana and Petrović, Aleksandra and Gvozdenac, Sonja and Starović, Mira and Kuzmanović, Slobodan and Aleksić, Goran",
year = "2019",
abstract = "The undemanding LC-MS/MS method was developed for the synchronized analysis of atropine and scopolamine in maize crop. The dSPE was carried out with 1% acetic acid in acetonitrile/water and a mixture of magnesium sulphate, sodium chloride and sodium citrate. The analytes were separated on a Zorbax XDB C18 column using methanol/water as the mobile phase in gradient mode. The detection was done using a tandem masss spectrometry (MS/MS) in the positive ESI. The tropane alkaloids exhibited excellent linearity in the range of 2-20 µg/kg with the LOQ of 5 µg/kg for maize. The extraction recoveries of atropine and scopolamine were 65.7 and 85.5% with the intraday RSDr 10.25 and 4.29%, respectively. The validated method was appled to real maze samples. One sample contained 18.8 µg/kg of atropine and 6.3 µg/kg of scopolamine.",
publisher = "Novi Sad : Univerzitet u Novom Sadu - Poljoprivredni fakultet, Novi Sad : Naučni institut za ratarstvo i povrtarstvo",
journal = "Savremena poljoprivreda",
title = "LC-MS/MS Determination of Tropane Alkaloids in Maize Crop",
pages = "226-221",
number = "3-4",
volume = "67",
doi = "10.1515/contagri-2018-0032, 10.1515/contagri-2018-0032"
}
Vuković, G., Bursić, V., Stojanović, T., Petrović, A., Gvozdenac, S., Starović, M., Kuzmanović, S.,& Aleksić, G.. (2019). LC-MS/MS Determination of Tropane Alkaloids in Maize Crop. in Savremena poljoprivreda
Novi Sad : Univerzitet u Novom Sadu - Poljoprivredni fakultet., 67(3-4), 221-226.
https://doi.org/10.1515/contagri-2018-0032
Vuković G, Bursić V, Stojanović T, Petrović A, Gvozdenac S, Starović M, Kuzmanović S, Aleksić G. LC-MS/MS Determination of Tropane Alkaloids in Maize Crop. in Savremena poljoprivreda. 2019;67(3-4):221-226.
doi:10.1515/contagri-2018-0032 .
