LC-MS/MS Determination of Tropane Alkaloids in Maize Crop
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2019
Authors
Vuković, GoricaBursić, Vojislava
Stojanović, Tijana
Petrović, Aleksandra
Gvozdenac, Sonja
Starović, Mira

Kuzmanović, Slobodan
Aleksić, Goran
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The undemanding LC-MS/MS method was developed for the synchronized analysis of atropine and scopolamine in maize crop. The dSPE was carried out with 1% acetic acid in acetonitrile/water and a mixture of magnesium sulphate, sodium chloride and sodium citrate. The analytes were separated on a Zorbax XDB C18 column using methanol/water as the mobile phase in gradient mode. The detection was done using a tandem masss spectrometry (MS/MS) in the positive ESI. The tropane alkaloids exhibited excellent linearity in the range of 2-20 µg/kg with the LOQ of 5 µg/kg for maize. The extraction recoveries of atropine and scopolamine were 65.7 and 85.5% with the intraday RSDr 10.25 and 4.29%, respectively. The validated method was appled to real maze samples. One sample contained 18.8 µg/kg of atropine and 6.3 µg/kg of scopolamine.
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Savremena poljoprivreda, 2019, 67, 3-4, 221-226Publisher:
- Novi Sad : Univerzitet u Novom Sadu - Poljoprivredni fakultet
- Novi Sad : Naučni institut za ratarstvo i povrtarstvo
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IZBISTY - JOUR AU - Vuković, Gorica AU - Bursić, Vojislava AU - Stojanović, Tijana AU - Petrović, Aleksandra AU - Gvozdenac, Sonja AU - Starović, Mira AU - Kuzmanović, Slobodan AU - Aleksić, Goran PY - 2019 UR - https://plantarum.izbis.bg.ac.rs/handle/123456789/609 AB - The undemanding LC-MS/MS method was developed for the synchronized analysis of atropine and scopolamine in maize crop. The dSPE was carried out with 1% acetic acid in acetonitrile/water and a mixture of magnesium sulphate, sodium chloride and sodium citrate. The analytes were separated on a Zorbax XDB C18 column using methanol/water as the mobile phase in gradient mode. The detection was done using a tandem masss spectrometry (MS/MS) in the positive ESI. The tropane alkaloids exhibited excellent linearity in the range of 2-20 µg/kg with the LOQ of 5 µg/kg for maize. The extraction recoveries of atropine and scopolamine were 65.7 and 85.5% with the intraday RSDr 10.25 and 4.29%, respectively. The validated method was appled to real maze samples. One sample contained 18.8 µg/kg of atropine and 6.3 µg/kg of scopolamine. PB - Novi Sad : Univerzitet u Novom Sadu - Poljoprivredni fakultet PB - Novi Sad : Naučni institut za ratarstvo i povrtarstvo T2 - Savremena poljoprivreda T1 - LC-MS/MS Determination of Tropane Alkaloids in Maize Crop EP - 226 IS - 3-4 SP - 221 VL - 67 DO - 10.1515/contagri-2018-0032 DO - 10.1515/contagri-2018-0032 ER -
@article{ author = "Vuković, Gorica and Bursić, Vojislava and Stojanović, Tijana and Petrović, Aleksandra and Gvozdenac, Sonja and Starović, Mira and Kuzmanović, Slobodan and Aleksić, Goran", year = "2019", abstract = "The undemanding LC-MS/MS method was developed for the synchronized analysis of atropine and scopolamine in maize crop. The dSPE was carried out with 1% acetic acid in acetonitrile/water and a mixture of magnesium sulphate, sodium chloride and sodium citrate. The analytes were separated on a Zorbax XDB C18 column using methanol/water as the mobile phase in gradient mode. The detection was done using a tandem masss spectrometry (MS/MS) in the positive ESI. The tropane alkaloids exhibited excellent linearity in the range of 2-20 µg/kg with the LOQ of 5 µg/kg for maize. The extraction recoveries of atropine and scopolamine were 65.7 and 85.5% with the intraday RSDr 10.25 and 4.29%, respectively. The validated method was appled to real maze samples. One sample contained 18.8 µg/kg of atropine and 6.3 µg/kg of scopolamine.", publisher = "Novi Sad : Univerzitet u Novom Sadu - Poljoprivredni fakultet, Novi Sad : Naučni institut za ratarstvo i povrtarstvo", journal = "Savremena poljoprivreda", title = "LC-MS/MS Determination of Tropane Alkaloids in Maize Crop", pages = "226-221", number = "3-4", volume = "67", doi = "10.1515/contagri-2018-0032, 10.1515/contagri-2018-0032" }
Vuković, G., Bursić, V., Stojanović, T., Petrović, A., Gvozdenac, S., Starović, M., Kuzmanović, S.,& Aleksić, G.. (2019). LC-MS/MS Determination of Tropane Alkaloids in Maize Crop. in Savremena poljoprivreda Novi Sad : Univerzitet u Novom Sadu - Poljoprivredni fakultet., 67(3-4), 221-226. https://doi.org/10.1515/contagri-2018-0032
Vuković G, Bursić V, Stojanović T, Petrović A, Gvozdenac S, Starović M, Kuzmanović S, Aleksić G. LC-MS/MS Determination of Tropane Alkaloids in Maize Crop. in Savremena poljoprivreda. 2019;67(3-4):221-226. doi:10.1515/contagri-2018-0032 .
Vuković, Gorica, Bursić, Vojislava, Stojanović, Tijana, Petrović, Aleksandra, Gvozdenac, Sonja, Starović, Mira, Kuzmanović, Slobodan, Aleksić, Goran, "LC-MS/MS Determination of Tropane Alkaloids in Maize Crop" in Savremena poljoprivreda, 67, no. 3-4 (2019):221-226, https://doi.org/10.1515/contagri-2018-0032 . .