Pectobacterium is a diverse genus which comprises of multiple destructive bacterial species which cause soft rot/blackleg/wilt disease complex in a wide variety of crops by employing high levels of virulence factors. During the 2018, 2019 and 2020 potato growing seasons, numerous outbreaks of bacterial wilt, stem blackleg and tuber soft rot were recorded, and symptomatic plant samples from ten localities in the Province of Vojvodina (Serbia) were collected and analysed. Bacterial soft-rot pathogens were detected in 63 samples using genus and species-specific primers. Through 16S rRNA Sanger sequencing of 19 representative isolates, the identity of P. brasiliense (73.7%), P. punjabense (15.8%), and P. carotovorum (10.5%) species were revealed. To further validate the identification, genotypic profiling of Pectobacterium strains using rep-PCR (ERIC, BOX, REP) was conducted for 25 selected isolates and the phylogenetic assessment based on four selected housekeeping genes (gyrA, recA, rpoA..., and rpoS). Physiological and biochemical properties were analysed using basic microbiological tests and VITEK® 2 GN card, and pathogenicity was confirmed on cv. VR808 and cv. Desiree potato tubers and plants. This study confirmed the distinctiveness of the newly described P. punjabense in Serbia as well as the high diversity of Pectobacterium brasiliense and Pectobacterium carotovorum species in Serbia.
TY - JOUR
AU - Loc, Marta
AU - Milošević, Dragana
AU - Ivanović, Žarko
AU - Ignjatov, Maja
AU - Budakov, Dragana
AU - Grahovac, Jovana
AU - Grahovac, Mila
PY - 2022
UR - https://plantarum.izbis.bg.ac.rs/handle/123456789/685
AB - Pectobacterium is a diverse genus which comprises of multiple destructive bacterial species which cause soft rot/blackleg/wilt disease complex in a wide variety of crops by employing high levels of virulence factors. During the 2018, 2019 and 2020 potato growing seasons, numerous outbreaks of bacterial wilt, stem blackleg and tuber soft rot were recorded, and symptomatic plant samples from ten localities in the Province of Vojvodina (Serbia) were collected and analysed. Bacterial soft-rot pathogens were detected in 63 samples using genus and species-specific primers. Through 16S rRNA Sanger sequencing of 19 representative isolates, the identity of P. brasiliense (73.7%), P. punjabense (15.8%), and P. carotovorum (10.5%) species were revealed. To further validate the identification, genotypic profiling of Pectobacterium strains using rep-PCR (ERIC, BOX, REP) was conducted for 25 selected isolates and the phylogenetic assessment based on four selected housekeeping genes (gyrA, recA, rpoA, and rpoS). Physiological and biochemical properties were analysed using basic microbiological tests and VITEK® 2 GN card, and pathogenicity was confirmed on cv. VR808 and cv. Desiree potato tubers and plants. This study confirmed the distinctiveness of the newly described P. punjabense in Serbia as well as the high diversity of Pectobacterium brasiliense and Pectobacterium carotovorum species in Serbia.
PB - MDPI
T2 - Microorganisms
T1 - Genetic Diversity of Pectobacterium spp. on Potato in Serbia
IS - 9
VL - 10
DO - 10.3390/microorganisms10091840
ER -
@article{
author = "Loc, Marta and Milošević, Dragana and Ivanović, Žarko and Ignjatov, Maja and Budakov, Dragana and Grahovac, Jovana and Grahovac, Mila",
year = "2022",
abstract = "Pectobacterium is a diverse genus which comprises of multiple destructive bacterial species which cause soft rot/blackleg/wilt disease complex in a wide variety of crops by employing high levels of virulence factors. During the 2018, 2019 and 2020 potato growing seasons, numerous outbreaks of bacterial wilt, stem blackleg and tuber soft rot were recorded, and symptomatic plant samples from ten localities in the Province of Vojvodina (Serbia) were collected and analysed. Bacterial soft-rot pathogens were detected in 63 samples using genus and species-specific primers. Through 16S rRNA Sanger sequencing of 19 representative isolates, the identity of P. brasiliense (73.7%), P. punjabense (15.8%), and P. carotovorum (10.5%) species were revealed. To further validate the identification, genotypic profiling of Pectobacterium strains using rep-PCR (ERIC, BOX, REP) was conducted for 25 selected isolates and the phylogenetic assessment based on four selected housekeeping genes (gyrA, recA, rpoA, and rpoS). Physiological and biochemical properties were analysed using basic microbiological tests and VITEK® 2 GN card, and pathogenicity was confirmed on cv. VR808 and cv. Desiree potato tubers and plants. This study confirmed the distinctiveness of the newly described P. punjabense in Serbia as well as the high diversity of Pectobacterium brasiliense and Pectobacterium carotovorum species in Serbia.",
publisher = "MDPI",
journal = "Microorganisms",
title = "Genetic Diversity of Pectobacterium spp. on Potato in Serbia",
number = "9",
volume = "10",
doi = "10.3390/microorganisms10091840"
}
Loc, M., Milošević, D., Ivanović, Ž., Ignjatov, M., Budakov, D., Grahovac, J.,& Grahovac, M.. (2022). Genetic Diversity of Pectobacterium spp. on Potato in Serbia. in Microorganisms
MDPI., 10(9).
https://doi.org/10.3390/microorganisms10091840
Loc M, Milošević D, Ivanović Ž, Ignjatov M, Budakov D, Grahovac J, Grahovac M. Genetic Diversity of Pectobacterium spp. on Potato in Serbia. in Microorganisms. 2022;10(9).
doi:10.3390/microorganisms10091840 .
Loc, Marta, Milošević, Dragana, Ivanović, Žarko, Ignjatov, Maja, Budakov, Dragana, Grahovac, Jovana, Grahovac, Mila, "Genetic Diversity of Pectobacterium spp. on Potato in Serbia" in Microorganisms, 10, no. 9 (2022),
https://doi.org/10.3390/microorganisms10091840 . .
