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Grapevine rupestris stem pitting virus: a new pathogen of grapevine in Serbia

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Authors
Stanković, Ivana
Zečević, Katarina
Delibašić, Goran
Jovic, Jelena
Tosevski, Ivo
Krstić, Branka
Article (Published version)
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Abstract
Grapevine rupestris stem pitting-associated virus (GRSPaV), a member of the genus Foveavirus, is a commonly detected grapevine virus around the world. Despite the broad and global distribution of GRSPaV, no information on its presence in Serbia had been available before this study. In October 2018, 3-year-old grapevine plants of ‘Cabernet Sauvignon’ showing reduced growth and leaf reddening were observed in the vicinity of Krnjevo locality. The results of an mRT-PCR test showed that only samples prepared from cortical scrapings of mature symptomatic grapevine canes yielded an amplicon of 155 bp that confirmed the presence of GRSPaV. No amplification products were recorded in samples prepared from basal leaves or petioles. A serological assay confirmed the presence of GRSPaV in all tested mature cane samples. For further analyses, the CP (coat protein) genes of two selected isolates (VL8 and VL15) of naturally infected grapevine canes were partially sequenced and six sequence variants w...ere determined, showing a certain variability among them. The constructed phylogenetic tree revealed the grouping of Serbian sequence variants into separate subgroups within the same phylogenetic lineage. This is the first report of GRSPaV present in Serbia. Further monitoring and increased phytosanitary control measures are needed to prevent potential spreading and introduction of new virus variants.

Keywords:
Grapevine / Grapevine rupestris stem pitting-associated virus / Multiplex RT-PCR / CP gene / Phylogenetic analysis
Source:
JOURNAL OF PLANT DISEASES AND PROTECTION, 2022
Publisher:
  • Springer
Funding / projects:
  • Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 200010 (Institute for Plant Protection and Environment, Belgrade) (RS-200010)
  • Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 200116 (University of Belgrade, Faculty of Agriculture) (RS-200116)

DOI: 10.1007/s41348-022-00656-x

ISSN: 1861-3829; 1861-3837

WoS: 000836095400001

Scopus: 2-s2.0-85135568209
[ Google Scholar ]
URI
https://plantarum.izbis.bg.ac.rs/handle/123456789/692
Collections
  • Radovi istraživača / Researchers' publications
Institution/Community
IZBIS
TY  - JOUR
AU  - Stanković, Ivana
AU  - Zečević, Katarina
AU  - Delibašić, Goran
AU  - Jovic, Jelena
AU  - Tosevski, Ivo
AU  - Krstić, Branka
PY  - 2022
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/692
AB  - Grapevine rupestris stem pitting-associated virus (GRSPaV), a member of the genus Foveavirus, is a commonly detected grapevine virus around the world. Despite the broad and global distribution of GRSPaV, no information on its presence in Serbia had been available before this study. In October 2018, 3-year-old grapevine plants of ‘Cabernet Sauvignon’ showing reduced growth and leaf reddening were observed in the vicinity of Krnjevo locality. The results of an mRT-PCR test showed that only samples prepared from cortical scrapings of mature symptomatic grapevine canes yielded an amplicon of 155 bp that confirmed the presence of GRSPaV. No amplification products were recorded in samples prepared from basal leaves or petioles. A serological assay confirmed the presence of GRSPaV in all tested mature cane samples. For further analyses, the CP (coat protein) genes of two selected isolates (VL8 and VL15) of naturally infected grapevine canes were partially sequenced and six sequence variants were determined, showing a certain variability among them. The constructed phylogenetic tree revealed the grouping of Serbian sequence variants into separate subgroups within the same phylogenetic lineage. This is the first report of GRSPaV present in Serbia. Further monitoring and increased phytosanitary control measures are needed to prevent potential spreading and introduction of new virus variants.
PB  - Springer
T2  - JOURNAL OF PLANT DISEASES AND PROTECTION
T1  - Grapevine rupestris stem pitting virus: a new pathogen of grapevine in Serbia
DO  - 10.1007/s41348-022-00656-x
ER  - 
@article{
author = "Stanković, Ivana and Zečević, Katarina and Delibašić, Goran and Jovic, Jelena and Tosevski, Ivo and Krstić, Branka",
year = "2022",
abstract = "Grapevine rupestris stem pitting-associated virus (GRSPaV), a member of the genus Foveavirus, is a commonly detected grapevine virus around the world. Despite the broad and global distribution of GRSPaV, no information on its presence in Serbia had been available before this study. In October 2018, 3-year-old grapevine plants of ‘Cabernet Sauvignon’ showing reduced growth and leaf reddening were observed in the vicinity of Krnjevo locality. The results of an mRT-PCR test showed that only samples prepared from cortical scrapings of mature symptomatic grapevine canes yielded an amplicon of 155 bp that confirmed the presence of GRSPaV. No amplification products were recorded in samples prepared from basal leaves or petioles. A serological assay confirmed the presence of GRSPaV in all tested mature cane samples. For further analyses, the CP (coat protein) genes of two selected isolates (VL8 and VL15) of naturally infected grapevine canes were partially sequenced and six sequence variants were determined, showing a certain variability among them. The constructed phylogenetic tree revealed the grouping of Serbian sequence variants into separate subgroups within the same phylogenetic lineage. This is the first report of GRSPaV present in Serbia. Further monitoring and increased phytosanitary control measures are needed to prevent potential spreading and introduction of new virus variants.",
publisher = "Springer",
journal = "JOURNAL OF PLANT DISEASES AND PROTECTION",
title = "Grapevine rupestris stem pitting virus: a new pathogen of grapevine in Serbia",
doi = "10.1007/s41348-022-00656-x"
}
Stanković, I., Zečević, K., Delibašić, G., Jovic, J., Tosevski, I.,& Krstić, B.. (2022). Grapevine rupestris stem pitting virus: a new pathogen of grapevine in Serbia. in JOURNAL OF PLANT DISEASES AND PROTECTION
Springer..
https://doi.org/10.1007/s41348-022-00656-x
Stanković I, Zečević K, Delibašić G, Jovic J, Tosevski I, Krstić B. Grapevine rupestris stem pitting virus: a new pathogen of grapevine in Serbia. in JOURNAL OF PLANT DISEASES AND PROTECTION. 2022;.
doi:10.1007/s41348-022-00656-x .
Stanković, Ivana, Zečević, Katarina, Delibašić, Goran, Jovic, Jelena, Tosevski, Ivo, Krstić, Branka, "Grapevine rupestris stem pitting virus: a new pathogen of grapevine in Serbia" in JOURNAL OF PLANT DISEASES AND PROTECTION (2022),
https://doi.org/10.1007/s41348-022-00656-x . .

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