Symptomatology, (Co)occurrence and Differential Diagnostic PCR Identification of ‘Ca. Phytoplasma solani’ and ‘Ca. Phytoplasma convolvuli’ in Field Bindweed
Аутори
Jovic, JelenaMarinković, Slavica
Jakovljević, Miljana
Krstić, Oliver
Cvrković, Tatjana
Mitrović, Milana
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Field bindweed (Convolvulus arvensis) is one of the major natural plant hosts and reservoirs
of ‘Candidatus Phytoplasma solani’ (‘Ca. P. solani’), the causal agent of plant diseases in diverse
agricultural crops, including Bois noir (BN) disease of grapevine. Phylogenetically, the most closely
related phytoplasma to ‘Ca. P. solani’, the ‘Ca. P. convolvuli’, induces disease in field bindweed that
is known by its symptoms as bindweed yellows (BY). The occurrence, coinfection and symptoms
association of the two phytoplasmas in shared host plants were the subject of this study. Specific
primers for the amplification of the elongation factor Tu gene (tuf ) were developed for the identification
of ‘Ca. P. convolvuli’ (by conventional nested PCR), as well as primers for simultaneous
detection of ‘Ca. P. solani’ and ‘Ca. P. convolvuli’ by duplex SYBR Green real-time PCR. Among
symptomatic bindweed plants, 25 and 41% were infected with a single phytoplasma species, ‘Ca.
P. solani’ and... ‘Ca. P. convolvuli’, respectively, while 34% were infected with both phytoplasmas.
None of the non-symptomatic control plants carried phytoplasma, while non-symptomatic plants
from our previous epidemiological studies in BN-affected vineyards were confirmed to be infected
solely with ‘Ca. P. solani’. Stamp gene typing revealed Rqg50 and Rqg31 ‘Ca. P. solani’ genotypes in
plants coinfected with ‘Ca. P. convolvuli’, while three diverse genotypes (Rqg50, GGY and Rpm35)
were identified in a single locality with symptomatic bindweeds infected solely with ‘Ca. P. solani’.
Variations in symptoms and their association with each of the phytoplasmas are described and
documented. The symptom of bushy appearance could be single out as specific for ‘Ca. P. convolvuli’
infection, while occurrence of ‘Ca. P. solani’ could not be unequivocally associated with specific
alterations in infected bindweeds. The results are discussed in the context of the epidemiological
and ecological complexity of ‘Ca. P. solani’-induced diseases and the relationship between the two
phytoplasma relatives in shared host plant.
Кључне речи:
bois noir / Convolvulus arvensis / duplex SYBR Green real-time PCR / end-point nested PCR / melting point temperature / phytoplasma / reservoir plant / symptomsИзвор:
Pathogens, 2021, 10, 2, 160-Издавач:
- MDPI Basel
Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200010 (Институт за заштиту биља и животну средину, Београд) (RS-MESTD-inst-2020-200010)
- STOLKit - A Toolkit for Risk Assessment Integration in Modeling a Management Strategy for Stolbur Phytoplasma Associated Diseases in Sustainable Agriculture (RS-ScienceFundRS-Promis-6060914)
DOI: 10.3390/pathogens10020160
ISSN: 2076-0817
PubMed: 33546447
WoS: 000622897700001
Scopus: 2-s2.0-85100581615
Институција/група
IZBISTY - JOUR AU - Jovic, Jelena AU - Marinković, Slavica AU - Jakovljević, Miljana AU - Krstić, Oliver AU - Cvrković, Tatjana AU - Mitrović, Milana PY - 2021 UR - https://plantarum.izbis.bg.ac.rs/handle/123456789/693 AB - Field bindweed (Convolvulus arvensis) is one of the major natural plant hosts and reservoirs of ‘Candidatus Phytoplasma solani’ (‘Ca. P. solani’), the causal agent of plant diseases in diverse agricultural crops, including Bois noir (BN) disease of grapevine. Phylogenetically, the most closely related phytoplasma to ‘Ca. P. solani’, the ‘Ca. P. convolvuli’, induces disease in field bindweed that is known by its symptoms as bindweed yellows (BY). The occurrence, coinfection and symptoms association of the two phytoplasmas in shared host plants were the subject of this study. Specific primers for the amplification of the elongation factor Tu gene (tuf ) were developed for the identification of ‘Ca. P. convolvuli’ (by conventional nested PCR), as well as primers for simultaneous detection of ‘Ca. P. solani’ and ‘Ca. P. convolvuli’ by duplex SYBR Green real-time PCR. Among symptomatic bindweed plants, 25 and 41% were infected with a single phytoplasma species, ‘Ca. P. solani’ and ‘Ca. P. convolvuli’, respectively, while 34% were infected with both phytoplasmas. None of the non-symptomatic control plants carried phytoplasma, while non-symptomatic plants from our previous epidemiological studies in BN-affected vineyards were confirmed to be infected solely with ‘Ca. P. solani’. Stamp gene typing revealed Rqg50 and Rqg31 ‘Ca. P. solani’ genotypes in plants coinfected with ‘Ca. P. convolvuli’, while three diverse genotypes (Rqg50, GGY and Rpm35) were identified in a single locality with symptomatic bindweeds infected solely with ‘Ca. P. solani’. Variations in symptoms and their association with each of the phytoplasmas are described and documented. The symptom of bushy appearance could be single out as specific for ‘Ca. P. convolvuli’ infection, while occurrence of ‘Ca. P. solani’ could not be unequivocally associated with specific alterations in infected bindweeds. The results are discussed in the context of the epidemiological and ecological complexity of ‘Ca. P. solani’-induced diseases and the relationship between the two phytoplasma relatives in shared host plant. PB - MDPI Basel T2 - Pathogens T1 - Symptomatology, (Co)occurrence and Differential Diagnostic PCR Identification of ‘Ca. Phytoplasma solani’ and ‘Ca. Phytoplasma convolvuli’ in Field Bindweed IS - 2 SP - 160 VL - 10 DO - 10.3390/pathogens10020160 ER -
@article{ author = "Jovic, Jelena and Marinković, Slavica and Jakovljević, Miljana and Krstić, Oliver and Cvrković, Tatjana and Mitrović, Milana", year = "2021", abstract = "Field bindweed (Convolvulus arvensis) is one of the major natural plant hosts and reservoirs of ‘Candidatus Phytoplasma solani’ (‘Ca. P. solani’), the causal agent of plant diseases in diverse agricultural crops, including Bois noir (BN) disease of grapevine. Phylogenetically, the most closely related phytoplasma to ‘Ca. P. solani’, the ‘Ca. P. convolvuli’, induces disease in field bindweed that is known by its symptoms as bindweed yellows (BY). The occurrence, coinfection and symptoms association of the two phytoplasmas in shared host plants were the subject of this study. Specific primers for the amplification of the elongation factor Tu gene (tuf ) were developed for the identification of ‘Ca. P. convolvuli’ (by conventional nested PCR), as well as primers for simultaneous detection of ‘Ca. P. solani’ and ‘Ca. P. convolvuli’ by duplex SYBR Green real-time PCR. Among symptomatic bindweed plants, 25 and 41% were infected with a single phytoplasma species, ‘Ca. P. solani’ and ‘Ca. P. convolvuli’, respectively, while 34% were infected with both phytoplasmas. None of the non-symptomatic control plants carried phytoplasma, while non-symptomatic plants from our previous epidemiological studies in BN-affected vineyards were confirmed to be infected solely with ‘Ca. P. solani’. Stamp gene typing revealed Rqg50 and Rqg31 ‘Ca. P. solani’ genotypes in plants coinfected with ‘Ca. P. convolvuli’, while three diverse genotypes (Rqg50, GGY and Rpm35) were identified in a single locality with symptomatic bindweeds infected solely with ‘Ca. P. solani’. Variations in symptoms and their association with each of the phytoplasmas are described and documented. The symptom of bushy appearance could be single out as specific for ‘Ca. P. convolvuli’ infection, while occurrence of ‘Ca. P. solani’ could not be unequivocally associated with specific alterations in infected bindweeds. The results are discussed in the context of the epidemiological and ecological complexity of ‘Ca. P. solani’-induced diseases and the relationship between the two phytoplasma relatives in shared host plant.", publisher = "MDPI Basel", journal = "Pathogens", title = "Symptomatology, (Co)occurrence and Differential Diagnostic PCR Identification of ‘Ca. Phytoplasma solani’ and ‘Ca. Phytoplasma convolvuli’ in Field Bindweed", number = "2", pages = "160", volume = "10", doi = "10.3390/pathogens10020160" }
Jovic, J., Marinković, S., Jakovljević, M., Krstić, O., Cvrković, T.,& Mitrović, M.. (2021). Symptomatology, (Co)occurrence and Differential Diagnostic PCR Identification of ‘Ca. Phytoplasma solani’ and ‘Ca. Phytoplasma convolvuli’ in Field Bindweed. in Pathogens MDPI Basel., 10(2), 160. https://doi.org/10.3390/pathogens10020160
Jovic J, Marinković S, Jakovljević M, Krstić O, Cvrković T, Mitrović M. Symptomatology, (Co)occurrence and Differential Diagnostic PCR Identification of ‘Ca. Phytoplasma solani’ and ‘Ca. Phytoplasma convolvuli’ in Field Bindweed. in Pathogens. 2021;10(2):160. doi:10.3390/pathogens10020160 .
Jovic, Jelena, Marinković, Slavica, Jakovljević, Miljana, Krstić, Oliver, Cvrković, Tatjana, Mitrović, Milana, "Symptomatology, (Co)occurrence and Differential Diagnostic PCR Identification of ‘Ca. Phytoplasma solani’ and ‘Ca. Phytoplasma convolvuli’ in Field Bindweed" in Pathogens, 10, no. 2 (2021):160, https://doi.org/10.3390/pathogens10020160 . .