BACKGROUND Bačka (Vojvodina, Serbia) is one of the main potato-growing areas in Serbia. The presence of the stolbur-associated symptoms in the form of reddening and leaf rolling, shortened internodes, and aerial tuber formation were frequently noticed on the potato plants. Therefore, the identification of the causal agent of the disease was initiated, considering that presence of phytoplasmas was not earlier reported in Maglić locality. OBJECTIVES This research aimed to identify the causal agent of the phytoplasmas’ disease in potato field (unknown cultivar) in locality Maglić (Bačka) in 2021. METHODS Samples for the identification within the survey site consisted of pooled multiple diseased plants (leaves and tubers). Genomic DNA from the ten pooled samples was extracted using the Cetyltrimethyl ammonium bromide (CTAB) method. Identification was performed with polymerase chain reaction (PCR) using universal phytoplasma 16S rRNA primer pair P1/P7. The obtained sequences were checked fo...r identity with the deposited strains in the NCBI database using the BLASTn search tool. Further, Neighbour-joining (NJ) tree was made to reconstruct the phylogeny of potato strains in relation to deposited strains in the NCBI database. RESULTS PCR amplification performed with primer pair P1/P7 resulted in amplicon generation at 1800 bp for three (two from tubers and one from leaves) out of a total of ten tested samples. BLASTn analysis revealed 100% identity of potato strains with deposited strains of Candidatus Phytoplasma solani, the 16SrXII-A ribosom al subgroup. NJ phylogenetic analysis grouped potato strains in the same tree cluster with NCBI deposited strains of Ca. Phytoplasma solani.
Keywords:
potato / Candidatus Phytoplasma solani / 16S rRNA
Source:
FEMS Online Conference on Microbiology in association with Serbian Society of Microbiology, 30 June- 02 July 2022, 2022
Publisher:
Federation of European Microbiological Societies in association with the Serbian Society of Microbiology
TY - CONF
AU - Iličić, Renata
AU - Barać, Goran
AU - Marković, Sanja
AU - Jelušić, Aleksandra
AU - Stanković, Slaviša
AU - Popović Milovanović, Tatjana
PY - 2022
UR - https://plantarum.izbis.bg.ac.rs/handle/123456789/799
AB - BACKGROUND Bačka (Vojvodina, Serbia) is one of the main potato-growing areas in Serbia. The presence of the stolbur-associated symptoms in the form of reddening and leaf rolling, shortened internodes, and aerial tuber formation were frequently noticed on the potato plants. Therefore, the identification of the causal agent of the disease was initiated, considering that presence of phytoplasmas was not earlier reported in Maglić locality. OBJECTIVES This research aimed to identify the causal agent of the phytoplasmas’ disease in potato field (unknown cultivar) in locality Maglić (Bačka) in 2021. METHODS Samples for the identification within the survey site consisted of pooled multiple diseased plants (leaves and tubers). Genomic DNA from the ten pooled samples was extracted using the Cetyltrimethyl ammonium bromide (CTAB) method. Identification was performed with polymerase chain reaction (PCR) using universal phytoplasma 16S rRNA primer pair P1/P7. The obtained sequences were checked for identity with the deposited strains in the NCBI database using the BLASTn search tool. Further, Neighbour-joining (NJ) tree was made to reconstruct the phylogeny of potato strains in relation to deposited strains in the NCBI database. RESULTS PCR amplification performed with primer pair P1/P7 resulted in amplicon generation at 1800 bp for three (two from tubers and one from leaves) out of a total of ten tested samples. BLASTn analysis revealed 100% identity of potato strains with deposited strains of Candidatus Phytoplasma solani, the 16SrXII-A ribosom al subgroup. NJ phylogenetic analysis grouped potato strains in the same tree cluster with NCBI deposited strains of Ca. Phytoplasma solani.
PB - Federation of European Microbiological Societies in association with the Serbian Society of Microbiology
C3 - FEMS Online Conference on Microbiology in association with Serbian Society of Microbiology, 30 June- 02 July 2022
T1 - NEW DATA ON PRESENCE OF CANDIDATUS PHYTOPLASMA SOLANI ON POTATO IN VOJVODINA PROVINCE
ER -
@conference{
author = "Iličić, Renata and Barać, Goran and Marković, Sanja and Jelušić, Aleksandra and Stanković, Slaviša and Popović Milovanović, Tatjana",
year = "2022",
abstract = "BACKGROUND Bačka (Vojvodina, Serbia) is one of the main potato-growing areas in Serbia. The presence of the stolbur-associated symptoms in the form of reddening and leaf rolling, shortened internodes, and aerial tuber formation were frequently noticed on the potato plants. Therefore, the identification of the causal agent of the disease was initiated, considering that presence of phytoplasmas was not earlier reported in Maglić locality. OBJECTIVES This research aimed to identify the causal agent of the phytoplasmas’ disease in potato field (unknown cultivar) in locality Maglić (Bačka) in 2021. METHODS Samples for the identification within the survey site consisted of pooled multiple diseased plants (leaves and tubers). Genomic DNA from the ten pooled samples was extracted using the Cetyltrimethyl ammonium bromide (CTAB) method. Identification was performed with polymerase chain reaction (PCR) using universal phytoplasma 16S rRNA primer pair P1/P7. The obtained sequences were checked for identity with the deposited strains in the NCBI database using the BLASTn search tool. Further, Neighbour-joining (NJ) tree was made to reconstruct the phylogeny of potato strains in relation to deposited strains in the NCBI database. RESULTS PCR amplification performed with primer pair P1/P7 resulted in amplicon generation at 1800 bp for three (two from tubers and one from leaves) out of a total of ten tested samples. BLASTn analysis revealed 100% identity of potato strains with deposited strains of Candidatus Phytoplasma solani, the 16SrXII-A ribosom al subgroup. NJ phylogenetic analysis grouped potato strains in the same tree cluster with NCBI deposited strains of Ca. Phytoplasma solani.",
publisher = "Federation of European Microbiological Societies in association with the Serbian Society of Microbiology",
journal = "FEMS Online Conference on Microbiology in association with Serbian Society of Microbiology, 30 June- 02 July 2022",
title = "NEW DATA ON PRESENCE OF CANDIDATUS PHYTOPLASMA SOLANI ON POTATO IN VOJVODINA PROVINCE"
}
Iličić, R., Barać, G., Marković, S., Jelušić, A., Stanković, S.,& Popović Milovanović, T.. (2022). NEW DATA ON PRESENCE OF CANDIDATUS PHYTOPLASMA SOLANI ON POTATO IN VOJVODINA PROVINCE. in FEMS Online Conference on Microbiology in association with Serbian Society of Microbiology, 30 June- 02 July 2022
Federation of European Microbiological Societies in association with the Serbian Society of Microbiology..
Iličić R, Barać G, Marković S, Jelušić A, Stanković S, Popović Milovanović T. NEW DATA ON PRESENCE OF CANDIDATUS PHYTOPLASMA SOLANI ON POTATO IN VOJVODINA PROVINCE. in FEMS Online Conference on Microbiology in association with Serbian Society of Microbiology, 30 June- 02 July 2022. 2022;..
Iličić, Renata, Barać, Goran, Marković, Sanja, Jelušić, Aleksandra, Stanković, Slaviša, Popović Milovanović, Tatjana, "NEW DATA ON PRESENCE OF CANDIDATUS PHYTOPLASMA SOLANI ON POTATO IN VOJVODINA PROVINCE" in FEMS Online Conference on Microbiology in association with Serbian Society of Microbiology, 30 June- 02 July 2022 (2022).
