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In vitro assessment of the antagonistic potential of Bacillus spp. and Pseudomonas spp. against Xanthomonas campestris pv. campestris isolated from winter oilseed rape in Serbia

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Authors
Jelušić, Aleksandra
Berić, Tanja
Dimkić, Ivica
Mitrović, Petar
Stanković, Slaviša
Marković, Sanja
Popović Milovanović, Tatjana
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Abstract
Background: Yield and quality of oilseed rape and other Brassicacceae vegetables are affected with plant pathogenic bacterium Xanthomonas campestris pv. campestris (Xcc), both worldwide and in Serbia. Considering the losses that can arise as a consequence of the infection with this pathogen, there is an increasing need for finding the effective and environmentally safe method for its suppression. Objectives: With the aim to find a promising biological control agent for managing Xcc infection on winter oilseed rape in Serbia, this research was based on evaluation of the in vitro antagonistic potential of the Bacillus- and Pseudomonas-like isolates obtained from the phyllosphere and rhizosphere of the diseased and healthy winter oilseed rape plants. Methods: Well diffusion technique was used to evaluate the potential inhibitory activity of the whole cultures and cell-free supernatants of the 36 Bacillus- and Pseudomonas-like isolates, against the collection of Serbian winter oilseed rape... Xcc isolates. DNA of the isolates which were able to inhibit Xcc growth was amplified with the primers based on the sequences of tuf and gyrB genes for Bacillus- and Pseudomonas-like isolates, respectively and sequenced. Results: Nine Bacillus spp. isolates whose whole cultures showed antagonistic potential were identified as B. thuringiensis/B. cereus (5); B. valezensis (1); B. mobilis (1); B. megaterium (1) and B. halotolerans (1), while six Pseudomonas spp. isolates were identified as P. orientalis (4) and P. fluorescens (2). Only five isolates within three species (B. valezensis, B. megaterium and P. orientalis) had both, the activity of the whole culture and supernatant.

Keywords:
antagonism / Bacillus / Pseudomonas
Source:
FEMS Online Conference on Microbiology, 28-31.10.2020 133-133, 2020
Publisher:
  • Federation of European Microbiological Societies in association with the Serbian Society of Microbiology

ISBN: 978-86-914897-6-2

[ Google Scholar ]
URI
https://plantarum.izbis.bg.ac.rs/handle/123456789/813
Collections
  • Radovi istraživača / Researchers' publications
Institution/Community
IZBIS
TY  - CONF
AU  - Jelušić, Aleksandra
AU  - Berić, Tanja
AU  - Dimkić, Ivica
AU  - Mitrović, Petar
AU  - Stanković, Slaviša
AU  - Marković, Sanja
AU  - Popović Milovanović, Tatjana
PY  - 2020
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/813
AB  - Background: Yield and quality of oilseed rape and other Brassicacceae vegetables are affected with plant pathogenic bacterium Xanthomonas campestris pv. campestris (Xcc), both worldwide and in Serbia. Considering the losses that can arise as a consequence of the infection with this pathogen, there is an increasing need for finding the effective and environmentally safe method for its suppression. Objectives: With the aim to find a promising biological control agent for managing Xcc infection on winter oilseed rape in Serbia, this research was based on evaluation of the in vitro antagonistic potential of the Bacillus- and Pseudomonas-like isolates obtained from the phyllosphere and rhizosphere of the diseased and healthy winter oilseed rape plants. Methods: Well diffusion technique was used to evaluate the potential inhibitory activity of the whole cultures and cell-free supernatants of the 36 Bacillus- and Pseudomonas-like isolates, against the collection of Serbian winter oilseed rape Xcc isolates. DNA of the isolates which were able to inhibit Xcc growth was amplified with the primers based on the sequences of tuf and gyrB genes for Bacillus- and Pseudomonas-like isolates, respectively and sequenced. Results: Nine Bacillus spp. isolates whose whole cultures showed antagonistic potential were identified as B. thuringiensis/B. cereus (5); B. valezensis (1); B. mobilis (1); B. megaterium (1) and B. halotolerans (1), while six Pseudomonas spp. isolates were identified as P. orientalis (4) and P. fluorescens (2). Only five isolates within three species (B. valezensis, B. megaterium and P. orientalis) had both, the activity of the whole culture and supernatant.
PB  - Federation of European Microbiological Societies in association with the Serbian Society of Microbiology
C3  - FEMS Online Conference on Microbiology, 28-31.10.2020 133-133
T1  - In vitro assessment of the antagonistic potential of Bacillus spp. and Pseudomonas spp. against Xanthomonas campestris pv. campestris isolated from winter oilseed rape in Serbia
ER  - 
@conference{
author = "Jelušić, Aleksandra and Berić, Tanja and Dimkić, Ivica and Mitrović, Petar and Stanković, Slaviša and Marković, Sanja and Popović Milovanović, Tatjana",
year = "2020",
abstract = "Background: Yield and quality of oilseed rape and other Brassicacceae vegetables are affected with plant pathogenic bacterium Xanthomonas campestris pv. campestris (Xcc), both worldwide and in Serbia. Considering the losses that can arise as a consequence of the infection with this pathogen, there is an increasing need for finding the effective and environmentally safe method for its suppression. Objectives: With the aim to find a promising biological control agent for managing Xcc infection on winter oilseed rape in Serbia, this research was based on evaluation of the in vitro antagonistic potential of the Bacillus- and Pseudomonas-like isolates obtained from the phyllosphere and rhizosphere of the diseased and healthy winter oilseed rape plants. Methods: Well diffusion technique was used to evaluate the potential inhibitory activity of the whole cultures and cell-free supernatants of the 36 Bacillus- and Pseudomonas-like isolates, against the collection of Serbian winter oilseed rape Xcc isolates. DNA of the isolates which were able to inhibit Xcc growth was amplified with the primers based on the sequences of tuf and gyrB genes for Bacillus- and Pseudomonas-like isolates, respectively and sequenced. Results: Nine Bacillus spp. isolates whose whole cultures showed antagonistic potential were identified as B. thuringiensis/B. cereus (5); B. valezensis (1); B. mobilis (1); B. megaterium (1) and B. halotolerans (1), while six Pseudomonas spp. isolates were identified as P. orientalis (4) and P. fluorescens (2). Only five isolates within three species (B. valezensis, B. megaterium and P. orientalis) had both, the activity of the whole culture and supernatant.",
publisher = "Federation of European Microbiological Societies in association with the Serbian Society of Microbiology",
journal = "FEMS Online Conference on Microbiology, 28-31.10.2020 133-133",
title = "In vitro assessment of the antagonistic potential of Bacillus spp. and Pseudomonas spp. against Xanthomonas campestris pv. campestris isolated from winter oilseed rape in Serbia"
}
Jelušić, A., Berić, T., Dimkić, I., Mitrović, P., Stanković, S., Marković, S.,& Popović Milovanović, T.. (2020). In vitro assessment of the antagonistic potential of Bacillus spp. and Pseudomonas spp. against Xanthomonas campestris pv. campestris isolated from winter oilseed rape in Serbia. in FEMS Online Conference on Microbiology, 28-31.10.2020 133-133
Federation of European Microbiological Societies in association with the Serbian Society of Microbiology..
Jelušić A, Berić T, Dimkić I, Mitrović P, Stanković S, Marković S, Popović Milovanović T. In vitro assessment of the antagonistic potential of Bacillus spp. and Pseudomonas spp. against Xanthomonas campestris pv. campestris isolated from winter oilseed rape in Serbia. in FEMS Online Conference on Microbiology, 28-31.10.2020 133-133. 2020;..
Jelušić, Aleksandra, Berić, Tanja, Dimkić, Ivica, Mitrović, Petar, Stanković, Slaviša, Marković, Sanja, Popović Milovanović, Tatjana, "In vitro assessment of the antagonistic potential of Bacillus spp. and Pseudomonas spp. against Xanthomonas campestris pv. campestris isolated from winter oilseed rape in Serbia" in FEMS Online Conference on Microbiology, 28-31.10.2020 133-133 (2020).

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