Evaluation of different pcr primers for identification of tumorigenic bacteria associated with grapevine crown gall
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Authors
Kuzmanović, NemanjaBiondi, E.
Ivanović, Milan
Prokić, Anđelka
Zlatković, Nevena
Bertaccini, Assunta
Obradović, Aleksa

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Rhizobium rhizogenes, may seriously impact production in nurseries and vineyards worldwide. Although rapid and efficient detection and identification of tumorigenic bacteria is facilitated by PCR-based methods, high genetic diversity of these pathogens may hinder use of these methods in the disease diagnosis. Therefore, reliability of 11 primer pairs targeting fragments located on Ti plasmid or chromosomal DNA was tested on extensive collection of All. vitis, A. tumefaciens complex and R. rhizogenes strains isolated from grapevine throughout the world. Only primers VCF3/VCR3 targeting virC gene located on Ti plasmid clearly and accurately identified all tested tumorigenic strains associated with grapevine crown gall. Moreover, this primer pair coupled with primers specific for chromosomal pehA gene (PGF/PGR) in duplex PCR, may be recommended as the method of choice for routine preliminary identification of tumorigenic strains and differentiation of All.
Keywords:
Allorhizobium vitis / Agrobacterium tumefaciens complex / Rhizobium rhizogenes / tumor-inducing plasmid / diagnosisSource:
Journal of Plant Pathology, 2016, 98, 2, 311-319Publisher:
- Springer
Funding / projects:
- Development of integrated management of harmful organisms in plant production in order to overcome resistance and to improve food quality and safety (RS-46008)
- Advancing research in agricultural and food sciences at Faculty of Agriculture, University of Belgrade (EU-316004)
DOI: 10.4454/JPP.V98I2.028
ISSN: 1125-4653
WoS: 000394511600016
Scopus: 2-s2.0-84976338438
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IZBISTY - JOUR AU - Kuzmanović, Nemanja AU - Biondi, E. AU - Ivanović, Milan AU - Prokić, Anđelka AU - Zlatković, Nevena AU - Bertaccini, Assunta AU - Obradović, Aleksa PY - 2016 UR - https://plantarum.izbis.bg.ac.rs/handle/123456789/862 AB - Rhizobium rhizogenes, may seriously impact production in nurseries and vineyards worldwide. Although rapid and efficient detection and identification of tumorigenic bacteria is facilitated by PCR-based methods, high genetic diversity of these pathogens may hinder use of these methods in the disease diagnosis. Therefore, reliability of 11 primer pairs targeting fragments located on Ti plasmid or chromosomal DNA was tested on extensive collection of All. vitis, A. tumefaciens complex and R. rhizogenes strains isolated from grapevine throughout the world. Only primers VCF3/VCR3 targeting virC gene located on Ti plasmid clearly and accurately identified all tested tumorigenic strains associated with grapevine crown gall. Moreover, this primer pair coupled with primers specific for chromosomal pehA gene (PGF/PGR) in duplex PCR, may be recommended as the method of choice for routine preliminary identification of tumorigenic strains and differentiation of All. PB - Springer T2 - Journal of Plant Pathology T1 - Evaluation of different pcr primers for identification of tumorigenic bacteria associated with grapevine crown gall EP - 319 IS - 2 SP - 311 VL - 98 DO - 10.4454/JPP.V98I2.028 ER -
@article{ author = "Kuzmanović, Nemanja and Biondi, E. and Ivanović, Milan and Prokić, Anđelka and Zlatković, Nevena and Bertaccini, Assunta and Obradović, Aleksa", year = "2016", abstract = "Rhizobium rhizogenes, may seriously impact production in nurseries and vineyards worldwide. Although rapid and efficient detection and identification of tumorigenic bacteria is facilitated by PCR-based methods, high genetic diversity of these pathogens may hinder use of these methods in the disease diagnosis. Therefore, reliability of 11 primer pairs targeting fragments located on Ti plasmid or chromosomal DNA was tested on extensive collection of All. vitis, A. tumefaciens complex and R. rhizogenes strains isolated from grapevine throughout the world. Only primers VCF3/VCR3 targeting virC gene located on Ti plasmid clearly and accurately identified all tested tumorigenic strains associated with grapevine crown gall. Moreover, this primer pair coupled with primers specific for chromosomal pehA gene (PGF/PGR) in duplex PCR, may be recommended as the method of choice for routine preliminary identification of tumorigenic strains and differentiation of All.", publisher = "Springer", journal = "Journal of Plant Pathology", title = "Evaluation of different pcr primers for identification of tumorigenic bacteria associated with grapevine crown gall", pages = "319-311", number = "2", volume = "98", doi = "10.4454/JPP.V98I2.028" }
Kuzmanović, N., Biondi, E., Ivanović, M., Prokić, A., Zlatković, N., Bertaccini, A.,& Obradović, A.. (2016). Evaluation of different pcr primers for identification of tumorigenic bacteria associated with grapevine crown gall. in Journal of Plant Pathology Springer., 98(2), 311-319. https://doi.org/10.4454/JPP.V98I2.028
Kuzmanović N, Biondi E, Ivanović M, Prokić A, Zlatković N, Bertaccini A, Obradović A. Evaluation of different pcr primers for identification of tumorigenic bacteria associated with grapevine crown gall. in Journal of Plant Pathology. 2016;98(2):311-319. doi:10.4454/JPP.V98I2.028 .
Kuzmanović, Nemanja, Biondi, E., Ivanović, Milan, Prokić, Anđelka, Zlatković, Nevena, Bertaccini, Assunta, Obradović, Aleksa, "Evaluation of different pcr primers for identification of tumorigenic bacteria associated with grapevine crown gall" in Journal of Plant Pathology, 98, no. 2 (2016):311-319, https://doi.org/10.4454/JPP.V98I2.028 . .