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Sorbus domestica i s. Torminalis novi domaćini Ewinia amylovora u Srbiji

dc.creatorGavrilović, Veljko
dc.creatorŽivković, Svetlana
dc.creatorIvanović, Žarko
dc.creatorVojinović, Milić
dc.date.accessioned2019-04-04T15:52:40Z
dc.date.available2019-04-04T15:52:40Z
dc.date.issued2008
dc.identifier.issn0372-7866
dc.identifier.urihttp://plantarum.izbis.bg.ac.rs/handle/123456789/86
dc.description.abstractFirelight symptoms were observed on ornamental plants Sorbs domestic (region of Niš, 2005) and S. terminals (Požega, 2007). Symptoms appeared as leaves and shoots blight followed with cancer forming on branches of S. domestic and shoot blight on S. terminals. Levant type single colony were collected after three days growth on nutrient agar enrich net with sucrose (SNA). Investigated strays produced HR in tobacco and necrosis of artificially inoculated unripe pear fruits followed by oozing of bacterial exudates. Symptoms similar to those registered in natural infections, were recorded on artificial inoculated S. domestic leaves and shoots. Based on the results of pathogen city tests, biochemical characteristics, ELISA test and PCR analysis it was confirmed that the investigated strains belonged to E. amylovora, widespread pathogen of poem fruit trees and ornamental plants in Serbia. This is the first report of E. amylovora as pathogen of Sorbs plant in Serbia.en
dc.description.abstractU radu su prikazani rezultati proučavanja izolovanih sojeva bakterije iz plamenjačom zahvaćenih mladara ukrasnih biljka Sorbus domestica i Sorbus torminalis. Proučavani sojevi su Gram negativni, ne stvaraju fluorescentni pigment na King-ovoj podlozi B, a glukozu metabolišu i oksidativno i fermentativno. Prouzrokuju HR duvana i nekrozu nesazrelih, inokulisanih plodova kruške (viljamova) praćenu obilnom produkcijom bakterijskog eksudata. Prvi simptomi na inokulisanim mladarima biljaka domaćina u očavaju se posle 2-3 dana, ispoljavaju se u vidu vlažnih pega koje se vremenom šire zahvatajući ga u celosti. Na osnovu patogenih, biohemijskih i seroloških odlika (ELISA) proučavanih izolata zaključeno je da patološke promene na ukrasnim biljkama Sorbus domestica i Sorbus torminalis prouzrokuje Erwinia amylovora. To je potvrđeno i primenom molekularne metode (PCR) korišćenjem specifičnog prajmera za bakteriju E. amylovora. Ovo je prvi nalaz E. amylovora na ukrsnim biljkama roda Sorbus u Srbiji.sr
dc.publisherInstitute for Plant Protection and Environment, Belgrade
dc.relationProjekat Ministarstva nauke Republike Srbije, br. 20051: Optimizacija primene hemijskih sredstava u zaštiti bilja povećanjem efikasnosti dijagnostičkih metoda i procene rizika pojave bolesti, štetočina i korova
dc.rightsopenAccess
dc.sourceZaštita bilja
dc.subjectSorbus domesticaen
dc.subjectSorbus torminalisen
dc.subjectErwinia amylovoraen
dc.subjectpathogenicityen
dc.subjectdetectionen
dc.subjectELISAen
dc.subjectPCRen
dc.subjectErwinia amylovorasr
dc.subjectSorbus domesticasr
dc.subjectSorbus torminalissr
dc.subjectpatogenostsr
dc.subjectdetekcija ELISAsr
dc.subjectPCRsr
dc.titleSorbus domestica and s. Torminalis - new hosts of Ewinia amylovora in Serbiaen
dc.titleSorbus domestica i s. Torminalis novi domaćini Ewinia amylovora u Srbijisr
dc.typearticle
dc.rights.licenseARR
dc.citation.epage79
dc.citation.issue1-4
dc.citation.other59(1-4): 69-79
dc.citation.spage69
dc.citation.volume59
dc.type.versionpublishedVersion
dc.identifier.fulltexthttp://plantarum.izbis.bg.ac.rs//bitstream/id/1670/84.pdf
dc.identifier.rcubconv_196


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