Reliability of identification of viruses in potato tubers

Abstract

Time of infection of potato tubers with PVY and PLRV (primary or secondary) and the part on tubers from which samples were taken for analisis have an influence on incontestable detection of viruses by DAS ELISA method. In the tubers of primary infected plants the presence of PVY and PLRV was detected in 35% and 75%, respectively. All tubers (100%) of the secondary infected plants indicated positive reaction on PVY and PLRV. The samples for detection of PVY and PLRV in tubers of primary infected plants have to be taken from rose end and heel end, respectively, when all tubers tested showed positive reaction.

Poreklo virusne infekcije (primarna ili sekundarna) i mesto uzimanja uzoraka na krtoli utiču na dokazivanje prisustva Y virusa krompira (PVY) i virusa uvijenosti lišća krompira (PLRV) pri testiranju krtola. U krtolama primarno zaraženih biljaka dokazano je prisustvo PVY u 35%, a PLRV u 75% slučajeva. Prisustvo ovih virusa dokazano je u 100 % testiranih krtola sekundarno zaraženih biljaka. Najpogodnije mesto za uzimanje uzoraka na krtoli za dokazivanje PVY je okce, a za dokazivanje PLRV pupčani deo krtole, jer je u njima dokazano prisustvo virusa u 100 % testiranih krtola primarno zaraženih biljaka.