Due to an overlapping host range, similar symptomatology and many common characteristics, Pseudomonas syringae pathovars originating from stone fruits can easily be misidentified. In order to select tests for rapid and efficient differentiation of P. s. pvs. syringae, morsprunorum and persicae, we studied the suitability and differentiating potential of some standard bacteriological and molecular methods. Differentiation of the strains was performed using LOPAT, GATTa and ice nucleation tests, nutrient sucrose broth growth and utilization of various carbon sources. PCR method enabled the detection of toxin-producing genes: syrB and syrD in P. s. pv. syringae, and cfl gene in P. s. pv. morsprunorum race 1. Syringomycin production by pv. syringae was confirmed in bioassay using Geotrichum candidum, Saccharomyces cerevisiae and Rhodotorula pilimanae as indicator organisms. Pathogenicity test on lemon and immature nectarine fruits, as well as on string bean pods, showed different intensity... of reaction of the inoculated material which could separate pv. syringae from the other two pathovars. PCR-based repetitive sequences, Rep-PCR with REP, ERIC and BOX primers revealed different genetic profiles within P. syringae pathovars.
Keywords:
Pseudomonas syringae / stone fruit / identification / PCR / Pseudomonas syringae
Source:
Pesticidi i fitomedicina, 2012, 27, 3, 219-229
Publisher:
Institute of Pesticides and Environmental Protection, Belgrade & Plant Protection Society of Serbia, Belgrade
TY - JOUR
AU - Gašić, Katarina
AU - Prokić, Anđelka
AU - Ivanović, Milan
AU - Kuzmanović, Nemanja
AU - Obradović, Aleksa
PY - 2012
UR - https://plantarum.izbis.bg.ac.rs/handle/123456789/905
AB - Due to an overlapping host range, similar symptomatology and many common characteristics, Pseudomonas syringae pathovars originating from stone fruits can easily be misidentified. In order to select tests for rapid and efficient differentiation of P. s. pvs. syringae, morsprunorum and persicae, we studied the suitability and differentiating potential of some standard bacteriological and molecular methods. Differentiation of the strains was performed using LOPAT, GATTa and ice nucleation tests, nutrient sucrose broth growth and utilization of various carbon sources. PCR method enabled the detection of toxin-producing genes: syrB and syrD in P. s. pv. syringae, and cfl gene in P. s. pv. morsprunorum race 1. Syringomycin production by pv. syringae was confirmed in bioassay using Geotrichum candidum, Saccharomyces cerevisiae and Rhodotorula pilimanae as indicator organisms. Pathogenicity test on lemon and immature nectarine fruits, as well as on string bean pods, showed different intensity of reaction of the inoculated material which could separate pv. syringae from the other two pathovars. PCR-based repetitive sequences, Rep-PCR with REP, ERIC and BOX primers revealed different genetic profiles within P. syringae pathovars.
PB - Institute of Pesticides and Environmental Protection, Belgrade & Plant Protection Society of Serbia, Belgrade
T2 - Pesticidi i fitomedicina
T1 - Differentiation of Pseudomonas syringae pathovars originating from stone fruits
EP - 229
IS - 3
SP - 219
VL - 27
DO - 10.2298/PIF1203219G
ER -
@article{
author = "Gašić, Katarina and Prokić, Anđelka and Ivanović, Milan and Kuzmanović, Nemanja and Obradović, Aleksa",
year = "2012",
abstract = "Due to an overlapping host range, similar symptomatology and many common characteristics, Pseudomonas syringae pathovars originating from stone fruits can easily be misidentified. In order to select tests for rapid and efficient differentiation of P. s. pvs. syringae, morsprunorum and persicae, we studied the suitability and differentiating potential of some standard bacteriological and molecular methods. Differentiation of the strains was performed using LOPAT, GATTa and ice nucleation tests, nutrient sucrose broth growth and utilization of various carbon sources. PCR method enabled the detection of toxin-producing genes: syrB and syrD in P. s. pv. syringae, and cfl gene in P. s. pv. morsprunorum race 1. Syringomycin production by pv. syringae was confirmed in bioassay using Geotrichum candidum, Saccharomyces cerevisiae and Rhodotorula pilimanae as indicator organisms. Pathogenicity test on lemon and immature nectarine fruits, as well as on string bean pods, showed different intensity of reaction of the inoculated material which could separate pv. syringae from the other two pathovars. PCR-based repetitive sequences, Rep-PCR with REP, ERIC and BOX primers revealed different genetic profiles within P. syringae pathovars.",
publisher = "Institute of Pesticides and Environmental Protection, Belgrade & Plant Protection Society of Serbia, Belgrade",
journal = "Pesticidi i fitomedicina",
title = "Differentiation of Pseudomonas syringae pathovars originating from stone fruits",
pages = "229-219",
number = "3",
volume = "27",
doi = "10.2298/PIF1203219G"
}
Gašić, K., Prokić, A., Ivanović, M., Kuzmanović, N.,& Obradović, A.. (2012). Differentiation of Pseudomonas syringae pathovars originating from stone fruits. in Pesticidi i fitomedicina
Institute of Pesticides and Environmental Protection, Belgrade & Plant Protection Society of Serbia, Belgrade., 27(3), 219-229.
https://doi.org/10.2298/PIF1203219G
Gašić K, Prokić A, Ivanović M, Kuzmanović N, Obradović A. Differentiation of Pseudomonas syringae pathovars originating from stone fruits. in Pesticidi i fitomedicina. 2012;27(3):219-229.
doi:10.2298/PIF1203219G .
Gašić, Katarina, Prokić, Anđelka, Ivanović, Milan, Kuzmanović, Nemanja, Obradović, Aleksa, "Differentiation of Pseudomonas syringae pathovars originating from stone fruits" in Pesticidi i fitomedicina, 27, no. 3 (2012):219-229,
https://doi.org/10.2298/PIF1203219G . .
