Differentiation of Pseudomonas syringae pathovars originating from stone fruits
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Due to an overlapping host range, similar symptomatology and many common characteristics, Pseudomonas syringae pathovars originating from stone fruits can easily be misidentified. In order to select tests for rapid and efficient differentiation of P. s. pvs. syringae, morsprunorum and persicae, we studied the suitability and differentiating potential of some standard bacteriological and molecular methods. Differentiation of the strains was performed using LOPAT, GATTa and ice nucleation tests, nutrient sucrose broth growth and utilization of various carbon sources. PCR method enabled the detection of toxin-producing genes: syrB and syrD in P. s. pv. syringae, and cfl gene in P. s. pv. morsprunorum race 1. Syringomycin production by pv. syringae was confirmed in bioassay using Geotrichum candidum, Saccharomyces cerevisiae and Rhodotorula pilimanae as indicator organisms. Pathogenicity test on lemon and immature nectarine fruits, as well as on string bean pods, showed different intensity... of reaction of the inoculated material which could separate pv. syringae from the other two pathovars. PCR-based repetitive sequences, Rep-PCR with REP, ERIC and BOX primers revealed different genetic profiles within P. syringae pathovars.
Кључне речи:
Pseudomonas syringae / stone fruit / identification / PCR / Pseudomonas syringaeИзвор:
Pesticidi i fitomedicina, 2012, 27, 3, 219-229Издавач:
- Institute of Pesticides and Environmental Protection, Belgrade & Plant Protection Society of Serbia, Belgrade
Финансирање / пројекти:
- Развој интегрисаних система управљања штетним организмима у биљној производњи са циљем превазилажења резистентности и унапређења квалитета и безбедности хране (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-46008)
Институција/група
IZBISTY - JOUR AU - Gašić, Katarina AU - Prokić, Anđelka AU - Ivanović, Milan AU - Kuzmanović, Nemanja AU - Obradović, Aleksa PY - 2012 UR - https://plantarum.izbis.bg.ac.rs/handle/123456789/905 AB - Due to an overlapping host range, similar symptomatology and many common characteristics, Pseudomonas syringae pathovars originating from stone fruits can easily be misidentified. In order to select tests for rapid and efficient differentiation of P. s. pvs. syringae, morsprunorum and persicae, we studied the suitability and differentiating potential of some standard bacteriological and molecular methods. Differentiation of the strains was performed using LOPAT, GATTa and ice nucleation tests, nutrient sucrose broth growth and utilization of various carbon sources. PCR method enabled the detection of toxin-producing genes: syrB and syrD in P. s. pv. syringae, and cfl gene in P. s. pv. morsprunorum race 1. Syringomycin production by pv. syringae was confirmed in bioassay using Geotrichum candidum, Saccharomyces cerevisiae and Rhodotorula pilimanae as indicator organisms. Pathogenicity test on lemon and immature nectarine fruits, as well as on string bean pods, showed different intensity of reaction of the inoculated material which could separate pv. syringae from the other two pathovars. PCR-based repetitive sequences, Rep-PCR with REP, ERIC and BOX primers revealed different genetic profiles within P. syringae pathovars. PB - Institute of Pesticides and Environmental Protection, Belgrade & Plant Protection Society of Serbia, Belgrade T2 - Pesticidi i fitomedicina T1 - Differentiation of Pseudomonas syringae pathovars originating from stone fruits EP - 229 IS - 3 SP - 219 VL - 27 DO - 10.2298/PIF1203219G ER -
@article{ author = "Gašić, Katarina and Prokić, Anđelka and Ivanović, Milan and Kuzmanović, Nemanja and Obradović, Aleksa", year = "2012", abstract = "Due to an overlapping host range, similar symptomatology and many common characteristics, Pseudomonas syringae pathovars originating from stone fruits can easily be misidentified. In order to select tests for rapid and efficient differentiation of P. s. pvs. syringae, morsprunorum and persicae, we studied the suitability and differentiating potential of some standard bacteriological and molecular methods. Differentiation of the strains was performed using LOPAT, GATTa and ice nucleation tests, nutrient sucrose broth growth and utilization of various carbon sources. PCR method enabled the detection of toxin-producing genes: syrB and syrD in P. s. pv. syringae, and cfl gene in P. s. pv. morsprunorum race 1. Syringomycin production by pv. syringae was confirmed in bioassay using Geotrichum candidum, Saccharomyces cerevisiae and Rhodotorula pilimanae as indicator organisms. Pathogenicity test on lemon and immature nectarine fruits, as well as on string bean pods, showed different intensity of reaction of the inoculated material which could separate pv. syringae from the other two pathovars. PCR-based repetitive sequences, Rep-PCR with REP, ERIC and BOX primers revealed different genetic profiles within P. syringae pathovars.", publisher = "Institute of Pesticides and Environmental Protection, Belgrade & Plant Protection Society of Serbia, Belgrade", journal = "Pesticidi i fitomedicina", title = "Differentiation of Pseudomonas syringae pathovars originating from stone fruits", pages = "229-219", number = "3", volume = "27", doi = "10.2298/PIF1203219G" }
Gašić, K., Prokić, A., Ivanović, M., Kuzmanović, N.,& Obradović, A.. (2012). Differentiation of Pseudomonas syringae pathovars originating from stone fruits. in Pesticidi i fitomedicina Institute of Pesticides and Environmental Protection, Belgrade & Plant Protection Society of Serbia, Belgrade., 27(3), 219-229. https://doi.org/10.2298/PIF1203219G
Gašić K, Prokić A, Ivanović M, Kuzmanović N, Obradović A. Differentiation of Pseudomonas syringae pathovars originating from stone fruits. in Pesticidi i fitomedicina. 2012;27(3):219-229. doi:10.2298/PIF1203219G .
Gašić, Katarina, Prokić, Anđelka, Ivanović, Milan, Kuzmanović, Nemanja, Obradović, Aleksa, "Differentiation of Pseudomonas syringae pathovars originating from stone fruits" in Pesticidi i fitomedicina, 27, no. 3 (2012):219-229, https://doi.org/10.2298/PIF1203219G . .