Show simple item record

Vegetativna inkompatibilnost izolata Phomopsis spp. izolovanih sa stabla šljive

dc.creatorŽivković, Svetlana
dc.creatorStojanović, Saša
dc.creatorGavrilović, Veljko
dc.creatorIvanović, Žarko
dc.date.accessioned2019-04-04T15:52:56Z
dc.date.available2019-04-04T15:52:56Z
dc.date.issued2008
dc.identifier.issn0372-7866
dc.identifier.urihttp://plantarum.izbis.bg.ac.rs/handle/123456789/94
dc.description.abstractIsolates of Phomopsis spp. obtained from plum trees (Prunus domestica L.) with symptoms of die-back. There were significant morpho-physiological and pathogenic differences between the isolates. The genetic variability (12 isolates of Phomopsis spp. and isolate of Photopia pernicious, which was used as control ) was proved by study of vegetative compatibility. These isolates were inoculated in Petri dishes in pairs in all possible cross combinations, on potato dextrose agar. After 5 and 10 days the interaction between colonies were scored according to appearance of contact lines of paired cultures. On the contact line on two different isolates distinct barrage zones of inhibition - vegetative incompatibility reaction. The barrage zones generally became black and sometimes showed formation of pycnidia. Necrotic parts of hyphen that formed line of reaction were observed. A compatible reaction was observed only when cultures of the same isolate were paired.en
dc.description.abstractIzolati Phomopsis spp. dobijeni sa grana i stabla šljive (Prunus domestica L.) sa simptomima sušenja i izumiranja ispoljili su značajne razlike u morfolo-fiziološkim i patogenim karakteristikama. Primenom metode vegetativne kompatibilnosti potvrđena je genetska varijabilnost 12 izolata Phomopsis spp. i kontrolnog izolata Phomopsis perniciosa. Isečci ispitivanih kultura zasejavani su u Petri kutije po parovima i u svim mogućim unakrsnim kombinacijama, na podlozi KGA. Očitavanje rezultata je vršeno petog i desetog dana i podrazumevalo je praćenje izgleda linije međusobnog kontakta uparivanih izolata. Nakon perioda inkubacije između ispitivanih kultura dolazi do formiranja crne reakcione linije - barijere, a u pojedinim slučajevima i reproduktivnih organa - piknida (reakcija vegetativne inkompatibilnosti). Delovi hifa koje sačinjavaju crnu reakcionu liniju bili su nekrotirani. Vegetativna kompatibilnost konstatovana je samo u slučajevima kada su izolati uparivani sami sa sobom.sr
dc.publisherInstitute for Plant Protection and Environment, Belgrade
dc.relationProjekat Ministarstva nauke Republike Srbije, br. 20051: Optimizacija primene hemijskih sredstava u zaštiti bilja povećanjem efikasnosti dijagnostičkih metoda i procene rizika pojave bolesti, štetočina i korova
dc.rightsopenAccess
dc.sourceZaštita bilja
dc.subjectPhomopsis spp.en
dc.subjectvegetative compatibility/incompatibilityen
dc.subjectplumen
dc.subjectPhomopsis spp.sr
dc.subjectvegetativna kompatibilnost/inkompatibilnostsr
dc.subjectšljivasr
dc.titleVegetative incompatibility of isolates of Phomopsis spp. from plum treesen
dc.titleVegetativna inkompatibilnost izolata Phomopsis spp. izolovanih sa stabla šljivesr
dc.typearticle
dc.rights.licenseARR
dc.citation.epage24
dc.citation.issue1-4
dc.citation.other59(1-4): 15-24
dc.citation.spage15
dc.citation.volume59
dc.type.versionpublishedVersion
dc.identifier.fulltexthttp://plantarum.izbis.bg.ac.rs//bitstream/id/1678/92.pdf
dc.identifier.rcubconv_195


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record