Molecular characteristics of phytoplasmas associated with Flavescence doree in clematis and grapevine and preliminary results on the role of Dictyophara europaea as a vector

2009
Authors
Filippin, LuisaJović, Jelena

Cvrković, Tatjana

Forte, Vally
Clair, D.
Toševski, Ivo

Boudon-Padieu, E.
Borgo, Michele
Angelini, Elisa
Article (Published version)

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Show full item recordAbstract
A survey was conducted over several years in Italy and the Balkans in order to gain an understanding of the relationship between the Flavescence doree (FD) phytoplasma isolates found in clematis and grapevine. A total of 399 clematis and 107 grapevine samples were analyzed. The results showed that 36% of the Clematis vitalba plant samples were infected by phytoplasmas which, in grapevine, are associated with FD, a quarantine disease in Europe. Infected clematis plants were also found in areas where FD phytoplasma had never previously been reported to infect grapevine, Such as Macedonia, Croatia and some areas of Italy and Serbia. Molecular data from three phytoplasma genomic fragments showed the presence of different FD phytoplasma isolates, all belonging to the 16SrV-C subgroup, including the Italian FD-C isolate, the isolate found in Serbia, an isolate similar to the French FD2000 and a new isolate typical of central Italy. A few clematis plants were infected with single nucleotide p...olymorphism, insertion or deletion mutants of the FD-C isolate. Of all the potential Hemipteran vector species surveyed in Italy and Serbia, only 18 of 527 Dictyophara europaea individuals tested proved to he infected with the FD phytoplasma. Preliminary transmission experiments showed that this species is able to transmit the FD phytoplasma from clematis to grapevine. The presence of FD-infected clematis and of D. europaea could, therefore, constitute a risk for FD epidemics in the European viticultural regions.
Keywords:
Clematis vitalba / Dictyophara europaea / grapevine yellows / molecular detection and diagnostics / Scaphoideus titanus / Vitis viniferaSource:
Plant Pathology, 2009, 58, 5, 826-837Publisher:
- Wiley-Blackwell Publishing, Inc, Malden
Funding / projects:
- Italian Ministry of Agriculture
- Serbian Ministry of Agriculture - 401-00-7839/2006-11/9
DOI: 10.1111/j.1365-3059.2009.02092.x
ISSN: 0032-0862
WoS: 000270592900003
Scopus: 2-s2.0-70249130178
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IZBISTY - JOUR AU - Filippin, Luisa AU - Jović, Jelena AU - Cvrković, Tatjana AU - Forte, Vally AU - Clair, D. AU - Toševski, Ivo AU - Boudon-Padieu, E. AU - Borgo, Michele AU - Angelini, Elisa PY - 2009 UR - https://plantarum.izbis.bg.ac.rs/handle/123456789/98 AB - A survey was conducted over several years in Italy and the Balkans in order to gain an understanding of the relationship between the Flavescence doree (FD) phytoplasma isolates found in clematis and grapevine. A total of 399 clematis and 107 grapevine samples were analyzed. The results showed that 36% of the Clematis vitalba plant samples were infected by phytoplasmas which, in grapevine, are associated with FD, a quarantine disease in Europe. Infected clematis plants were also found in areas where FD phytoplasma had never previously been reported to infect grapevine, Such as Macedonia, Croatia and some areas of Italy and Serbia. Molecular data from three phytoplasma genomic fragments showed the presence of different FD phytoplasma isolates, all belonging to the 16SrV-C subgroup, including the Italian FD-C isolate, the isolate found in Serbia, an isolate similar to the French FD2000 and a new isolate typical of central Italy. A few clematis plants were infected with single nucleotide polymorphism, insertion or deletion mutants of the FD-C isolate. Of all the potential Hemipteran vector species surveyed in Italy and Serbia, only 18 of 527 Dictyophara europaea individuals tested proved to he infected with the FD phytoplasma. Preliminary transmission experiments showed that this species is able to transmit the FD phytoplasma from clematis to grapevine. The presence of FD-infected clematis and of D. europaea could, therefore, constitute a risk for FD epidemics in the European viticultural regions. PB - Wiley-Blackwell Publishing, Inc, Malden T2 - Plant Pathology T1 - Molecular characteristics of phytoplasmas associated with Flavescence doree in clematis and grapevine and preliminary results on the role of Dictyophara europaea as a vector EP - 837 IS - 5 SP - 826 VL - 58 DO - 10.1111/j.1365-3059.2009.02092.x ER -
@article{ author = "Filippin, Luisa and Jović, Jelena and Cvrković, Tatjana and Forte, Vally and Clair, D. and Toševski, Ivo and Boudon-Padieu, E. and Borgo, Michele and Angelini, Elisa", year = "2009", abstract = "A survey was conducted over several years in Italy and the Balkans in order to gain an understanding of the relationship between the Flavescence doree (FD) phytoplasma isolates found in clematis and grapevine. A total of 399 clematis and 107 grapevine samples were analyzed. The results showed that 36% of the Clematis vitalba plant samples were infected by phytoplasmas which, in grapevine, are associated with FD, a quarantine disease in Europe. Infected clematis plants were also found in areas where FD phytoplasma had never previously been reported to infect grapevine, Such as Macedonia, Croatia and some areas of Italy and Serbia. Molecular data from three phytoplasma genomic fragments showed the presence of different FD phytoplasma isolates, all belonging to the 16SrV-C subgroup, including the Italian FD-C isolate, the isolate found in Serbia, an isolate similar to the French FD2000 and a new isolate typical of central Italy. A few clematis plants were infected with single nucleotide polymorphism, insertion or deletion mutants of the FD-C isolate. Of all the potential Hemipteran vector species surveyed in Italy and Serbia, only 18 of 527 Dictyophara europaea individuals tested proved to he infected with the FD phytoplasma. Preliminary transmission experiments showed that this species is able to transmit the FD phytoplasma from clematis to grapevine. The presence of FD-infected clematis and of D. europaea could, therefore, constitute a risk for FD epidemics in the European viticultural regions.", publisher = "Wiley-Blackwell Publishing, Inc, Malden", journal = "Plant Pathology", title = "Molecular characteristics of phytoplasmas associated with Flavescence doree in clematis and grapevine and preliminary results on the role of Dictyophara europaea as a vector", pages = "837-826", number = "5", volume = "58", doi = "10.1111/j.1365-3059.2009.02092.x" }
Filippin, L., Jović, J., Cvrković, T., Forte, V., Clair, D., Toševski, I., Boudon-Padieu, E., Borgo, M.,& Angelini, E.. (2009). Molecular characteristics of phytoplasmas associated with Flavescence doree in clematis and grapevine and preliminary results on the role of Dictyophara europaea as a vector. in Plant Pathology Wiley-Blackwell Publishing, Inc, Malden., 58(5), 826-837. https://doi.org/10.1111/j.1365-3059.2009.02092.x
Filippin L, Jović J, Cvrković T, Forte V, Clair D, Toševski I, Boudon-Padieu E, Borgo M, Angelini E. Molecular characteristics of phytoplasmas associated with Flavescence doree in clematis and grapevine and preliminary results on the role of Dictyophara europaea as a vector. in Plant Pathology. 2009;58(5):826-837. doi:10.1111/j.1365-3059.2009.02092.x .
Filippin, Luisa, Jović, Jelena, Cvrković, Tatjana, Forte, Vally, Clair, D., Toševski, Ivo, Boudon-Padieu, E., Borgo, Michele, Angelini, Elisa, "Molecular characteristics of phytoplasmas associated with Flavescence doree in clematis and grapevine and preliminary results on the role of Dictyophara europaea as a vector" in Plant Pathology, 58, no. 5 (2009):826-837, https://doi.org/10.1111/j.1365-3059.2009.02092.x . .