Isolation and characterization of Xanthomonas euvesicatoria bacteriophages
Апстракт
Host range, plaque morphology, thermal inactivation point, genome size and restriction fragment patterns of ten bacteriophage isolates originating from soil, pepper seed and irrigation water collected from five localities in Serbia were studied. The bacteriophage isolates were selected based on their specificity to Xanthomonas euvesicatoria, causal agent of bacterial spot of pepper. The phages had similar plaque morphology, except for two isolates producing a plaque-surrounding halo in culture of X. euvesicatoria strain KFB 189. Four phage isolates were inactivated at 70 degrees C and six at 71 degrees C. All phages had genome size of approximately 22 kb and were differentiated into four types by their EcoRI and BamHI restriction fragment patterns. Examination of two phages by transmission electron microscopy classified them as A1 morphotype members of the Myoviridae family, order Caudovirales. Although specific to X. euvesicatoria, the phages were differentiated into three groups base...d on their ability to lyse 59 strains of this bacterium. Adsorption rates and one-step growth curves were determined for each group representative phage isolates.
Кључне речи:
phage host range / restriction analysis / electron microscopyИзвор:
Journal of Plant Pathology, 2011, 93, 2, 415-423Издавач:
- Edizioni Ets, Pisa
Финансирање / пројекти:
Институција/група
IZBISTY - JOUR AU - Gašić, Katarina AU - Ivanović, Milan M AU - Ignjatov, Maja AU - Calić, A AU - Obradović, Aleksa PY - 2011 UR - https://plantarum.izbis.bg.ac.rs/handle/123456789/160 AB - Host range, plaque morphology, thermal inactivation point, genome size and restriction fragment patterns of ten bacteriophage isolates originating from soil, pepper seed and irrigation water collected from five localities in Serbia were studied. The bacteriophage isolates were selected based on their specificity to Xanthomonas euvesicatoria, causal agent of bacterial spot of pepper. The phages had similar plaque morphology, except for two isolates producing a plaque-surrounding halo in culture of X. euvesicatoria strain KFB 189. Four phage isolates were inactivated at 70 degrees C and six at 71 degrees C. All phages had genome size of approximately 22 kb and were differentiated into four types by their EcoRI and BamHI restriction fragment patterns. Examination of two phages by transmission electron microscopy classified them as A1 morphotype members of the Myoviridae family, order Caudovirales. Although specific to X. euvesicatoria, the phages were differentiated into three groups based on their ability to lyse 59 strains of this bacterium. Adsorption rates and one-step growth curves were determined for each group representative phage isolates. PB - Edizioni Ets, Pisa T2 - Journal of Plant Pathology T1 - Isolation and characterization of Xanthomonas euvesicatoria bacteriophages EP - 423 IS - 2 SP - 415 VL - 93 ER -
@article{ author = "Gašić, Katarina and Ivanović, Milan M and Ignjatov, Maja and Calić, A and Obradović, Aleksa", year = "2011", abstract = "Host range, plaque morphology, thermal inactivation point, genome size and restriction fragment patterns of ten bacteriophage isolates originating from soil, pepper seed and irrigation water collected from five localities in Serbia were studied. The bacteriophage isolates were selected based on their specificity to Xanthomonas euvesicatoria, causal agent of bacterial spot of pepper. The phages had similar plaque morphology, except for two isolates producing a plaque-surrounding halo in culture of X. euvesicatoria strain KFB 189. Four phage isolates were inactivated at 70 degrees C and six at 71 degrees C. All phages had genome size of approximately 22 kb and were differentiated into four types by their EcoRI and BamHI restriction fragment patterns. Examination of two phages by transmission electron microscopy classified them as A1 morphotype members of the Myoviridae family, order Caudovirales. Although specific to X. euvesicatoria, the phages were differentiated into three groups based on their ability to lyse 59 strains of this bacterium. Adsorption rates and one-step growth curves were determined for each group representative phage isolates.", publisher = "Edizioni Ets, Pisa", journal = "Journal of Plant Pathology", title = "Isolation and characterization of Xanthomonas euvesicatoria bacteriophages", pages = "423-415", number = "2", volume = "93" }
Gašić, K., Ivanović, M. M., Ignjatov, M., Calić, A.,& Obradović, A.. (2011). Isolation and characterization of Xanthomonas euvesicatoria bacteriophages. in Journal of Plant Pathology Edizioni Ets, Pisa., 93(2), 415-423.
Gašić K, Ivanović MM, Ignjatov M, Calić A, Obradović A. Isolation and characterization of Xanthomonas euvesicatoria bacteriophages. in Journal of Plant Pathology. 2011;93(2):415-423..
Gašić, Katarina, Ivanović, Milan M, Ignjatov, Maja, Calić, A, Obradović, Aleksa, "Isolation and characterization of Xanthomonas euvesicatoria bacteriophages" in Journal of Plant Pathology, 93, no. 2 (2011):415-423.