Molecular characterization of PCN populations from Serbia
Molekularna karakterizacija populacija CNK iz Srbije
Abstract
The morphology of potato cyst nematodes (PCN) was until recently almost the only way to identify these quarantine organisms. In the last two decades, molecular analyses contributed to faster and more efficient identification of two Globodera species (Globodera pallida and G. rostochiensis) and allowed insight into the genetic structure of those parts that were practically inaccessible by morphological studies. Molecular characterization was performed in ITS1-5.8S-ITS2 region. The comparison was made with sequences of different foreign PCN populations via NCBI GenBank database. The results of molecular studies showed similarities and differences between local and foreign PCN populations in the part of genome that was studied.
Morfologija cistolikih nematoda krompira (CNK) je do nedavno bila gotovo jedini način identifikacije ovih karantinskih organizama. Molekularne analize su u poslednje dve decenije doprinele bržoj i efikasnijoj identifikaciji dve Globodera vrste (Globodera pallida i G. rostochiensis) i omogućile uvid u genetiku strukturu onih delova koji su praktično bili nedostupni morfološkim studijama. Molekularna karakterizacija je urađena u ITS1-5.8S-ITS2 regionu. Poređenja su vršena sa sekvencama različitih stranih populacija CNK preko NCBI GenBank baze podataka. Rezultati molekularnih studija pokazuju sličnosti i razlike domaćih i stranih populacija CNK u proučavanom delu genoma.
Keywords:
molecular characterization / potato cyst nematodes / SerbiaSource:
Genetika, 2012, 44, 1, 189-200Publisher:
- Serbian Genetics Society
Funding / projects:
- Development of integrated approach in plant protection for control harmful organisms (RS-31018)
- New indigenous bacterial isolates Lysobacter and Pseudomonas as an important source of metabolites useful for biotechnology, plant growth stimulation and disease control: from isolates to inoculants (RS-46007)
DOI: 10.2298/GENSR1201189O
ISSN: 0534-0012
WoS: 000306166800018
Scopus: 2-s2.0-84866925246
Collections
Institution/Community
IZBISTY - JOUR AU - Oro, Violeta AU - Oro-Radovanović, Vesna PY - 2012 UR - https://plantarum.izbis.bg.ac.rs/handle/123456789/221 AB - The morphology of potato cyst nematodes (PCN) was until recently almost the only way to identify these quarantine organisms. In the last two decades, molecular analyses contributed to faster and more efficient identification of two Globodera species (Globodera pallida and G. rostochiensis) and allowed insight into the genetic structure of those parts that were practically inaccessible by morphological studies. Molecular characterization was performed in ITS1-5.8S-ITS2 region. The comparison was made with sequences of different foreign PCN populations via NCBI GenBank database. The results of molecular studies showed similarities and differences between local and foreign PCN populations in the part of genome that was studied. AB - Morfologija cistolikih nematoda krompira (CNK) je do nedavno bila gotovo jedini način identifikacije ovih karantinskih organizama. Molekularne analize su u poslednje dve decenije doprinele bržoj i efikasnijoj identifikaciji dve Globodera vrste (Globodera pallida i G. rostochiensis) i omogućile uvid u genetiku strukturu onih delova koji su praktično bili nedostupni morfološkim studijama. Molekularna karakterizacija je urađena u ITS1-5.8S-ITS2 regionu. Poređenja su vršena sa sekvencama različitih stranih populacija CNK preko NCBI GenBank baze podataka. Rezultati molekularnih studija pokazuju sličnosti i razlike domaćih i stranih populacija CNK u proučavanom delu genoma. PB - Serbian Genetics Society T2 - Genetika T1 - Molecular characterization of PCN populations from Serbia T1 - Molekularna karakterizacija populacija CNK iz Srbije EP - 200 IS - 1 SP - 189 VL - 44 DO - 10.2298/GENSR1201189O ER -
@article{ author = "Oro, Violeta and Oro-Radovanović, Vesna", year = "2012", abstract = "The morphology of potato cyst nematodes (PCN) was until recently almost the only way to identify these quarantine organisms. In the last two decades, molecular analyses contributed to faster and more efficient identification of two Globodera species (Globodera pallida and G. rostochiensis) and allowed insight into the genetic structure of those parts that were practically inaccessible by morphological studies. Molecular characterization was performed in ITS1-5.8S-ITS2 region. The comparison was made with sequences of different foreign PCN populations via NCBI GenBank database. The results of molecular studies showed similarities and differences between local and foreign PCN populations in the part of genome that was studied., Morfologija cistolikih nematoda krompira (CNK) je do nedavno bila gotovo jedini način identifikacije ovih karantinskih organizama. Molekularne analize su u poslednje dve decenije doprinele bržoj i efikasnijoj identifikaciji dve Globodera vrste (Globodera pallida i G. rostochiensis) i omogućile uvid u genetiku strukturu onih delova koji su praktično bili nedostupni morfološkim studijama. Molekularna karakterizacija je urađena u ITS1-5.8S-ITS2 regionu. Poređenja su vršena sa sekvencama različitih stranih populacija CNK preko NCBI GenBank baze podataka. Rezultati molekularnih studija pokazuju sličnosti i razlike domaćih i stranih populacija CNK u proučavanom delu genoma.", publisher = "Serbian Genetics Society", journal = "Genetika", title = "Molecular characterization of PCN populations from Serbia, Molekularna karakterizacija populacija CNK iz Srbije", pages = "200-189", number = "1", volume = "44", doi = "10.2298/GENSR1201189O" }
Oro, V.,& Oro-Radovanović, V.. (2012). Molecular characterization of PCN populations from Serbia. in Genetika Serbian Genetics Society., 44(1), 189-200. https://doi.org/10.2298/GENSR1201189O
Oro V, Oro-Radovanović V. Molecular characterization of PCN populations from Serbia. in Genetika. 2012;44(1):189-200. doi:10.2298/GENSR1201189O .
Oro, Violeta, Oro-Radovanović, Vesna, "Molecular characterization of PCN populations from Serbia" in Genetika, 44, no. 1 (2012):189-200, https://doi.org/10.2298/GENSR1201189O . .