A method for the rapid detection and identification of halo blight pathogen on common bean
Apstrakt
A diagnostic method based on nested-PCR, followed by ELISA and conventional bacteriology tests, for the rapid and reliable detection of halo blight pathogen Pseudomonas savastanoi pv. phaseolicola (Psp) collected from infected bean leaves and seeds is described. Psp formed white, small and flat colonies on nutrient agar medium, creamy white, flat and circular on Milk-Tween agar medium and light yellow, convex and shiny on modified sucrose peptone agar medium. Eighteen Gram-negative, catalase-positive and oxidase-negative strains were subjected to nested PCR with primers P 5.1/P 3.1 and P 5.2/P 3.2, which directed the amplification of the 450 bp target DNA fragment in all tested strains. According to the results of DAS-and PTA-ELISA with respect to reactivity to specific antibodies, all analyzed strains belonged to Psp bacterium. Pathogenicity was tested on bean pods and cotyledon leaves, on which greasy spots were formed. Psp did not cause hypersensitive reaction on the leaves of tobac...co and geranium. Strains produced levan, fluorescent pigment, oxidative metabolism of glucose, did not reduce nitrate, did not produce indole and H2S, did not hydrolyze starch, gelatin and esculin; they produced acid from glucose, mannose, sucrose and glycerol, and did not produce acid from maltose, starch, esculin, dulcite, sorbitol, inositol and erythritol.
Ključne reči:
Pseudomonas savastanoi pv. Phaseolicola / halo blight / bean / identificationIzvor:
Archives of Biological Sciences, 2014, 66, 4, 1393-1399Izdavač:
- University of Belgrade, University of Novi Sad
Napomena:
- This article has been retracted. Link to the retraction: http://dx.doi.org/10.2298/ABS150609074E
DOI: 10.2298/ABS1404393P
ISSN: 0354-4664
WoS: 000346477900013
Scopus: 2-s2.0-84921687597
Institucija/grupa
IZBISTY - JOUR AU - Popović, Tatjana AU - Balaž, Jelica AU - Stanković, Slaviša PY - 2014 UR - https://plantarum.izbis.bg.ac.rs/handle/123456789/345 AB - A diagnostic method based on nested-PCR, followed by ELISA and conventional bacteriology tests, for the rapid and reliable detection of halo blight pathogen Pseudomonas savastanoi pv. phaseolicola (Psp) collected from infected bean leaves and seeds is described. Psp formed white, small and flat colonies on nutrient agar medium, creamy white, flat and circular on Milk-Tween agar medium and light yellow, convex and shiny on modified sucrose peptone agar medium. Eighteen Gram-negative, catalase-positive and oxidase-negative strains were subjected to nested PCR with primers P 5.1/P 3.1 and P 5.2/P 3.2, which directed the amplification of the 450 bp target DNA fragment in all tested strains. According to the results of DAS-and PTA-ELISA with respect to reactivity to specific antibodies, all analyzed strains belonged to Psp bacterium. Pathogenicity was tested on bean pods and cotyledon leaves, on which greasy spots were formed. Psp did not cause hypersensitive reaction on the leaves of tobacco and geranium. Strains produced levan, fluorescent pigment, oxidative metabolism of glucose, did not reduce nitrate, did not produce indole and H2S, did not hydrolyze starch, gelatin and esculin; they produced acid from glucose, mannose, sucrose and glycerol, and did not produce acid from maltose, starch, esculin, dulcite, sorbitol, inositol and erythritol. PB - University of Belgrade, University of Novi Sad T2 - Archives of Biological Sciences T1 - A method for the rapid detection and identification of halo blight pathogen on common bean EP - 1399 IS - 4 SP - 1393 VL - 66 DO - 10.2298/ABS1404393P ER -
@article{ author = "Popović, Tatjana and Balaž, Jelica and Stanković, Slaviša", year = "2014", abstract = "A diagnostic method based on nested-PCR, followed by ELISA and conventional bacteriology tests, for the rapid and reliable detection of halo blight pathogen Pseudomonas savastanoi pv. phaseolicola (Psp) collected from infected bean leaves and seeds is described. Psp formed white, small and flat colonies on nutrient agar medium, creamy white, flat and circular on Milk-Tween agar medium and light yellow, convex and shiny on modified sucrose peptone agar medium. Eighteen Gram-negative, catalase-positive and oxidase-negative strains were subjected to nested PCR with primers P 5.1/P 3.1 and P 5.2/P 3.2, which directed the amplification of the 450 bp target DNA fragment in all tested strains. According to the results of DAS-and PTA-ELISA with respect to reactivity to specific antibodies, all analyzed strains belonged to Psp bacterium. Pathogenicity was tested on bean pods and cotyledon leaves, on which greasy spots were formed. Psp did not cause hypersensitive reaction on the leaves of tobacco and geranium. Strains produced levan, fluorescent pigment, oxidative metabolism of glucose, did not reduce nitrate, did not produce indole and H2S, did not hydrolyze starch, gelatin and esculin; they produced acid from glucose, mannose, sucrose and glycerol, and did not produce acid from maltose, starch, esculin, dulcite, sorbitol, inositol and erythritol.", publisher = "University of Belgrade, University of Novi Sad", journal = "Archives of Biological Sciences", title = "A method for the rapid detection and identification of halo blight pathogen on common bean", pages = "1399-1393", number = "4", volume = "66", doi = "10.2298/ABS1404393P" }
Popović, T., Balaž, J.,& Stanković, S.. (2014). A method for the rapid detection and identification of halo blight pathogen on common bean. in Archives of Biological Sciences University of Belgrade, University of Novi Sad., 66(4), 1393-1399. https://doi.org/10.2298/ABS1404393P
Popović T, Balaž J, Stanković S. A method for the rapid detection and identification of halo blight pathogen on common bean. in Archives of Biological Sciences. 2014;66(4):1393-1399. doi:10.2298/ABS1404393P .
Popović, Tatjana, Balaž, Jelica, Stanković, Slaviša, "A method for the rapid detection and identification of halo blight pathogen on common bean" in Archives of Biological Sciences, 66, no. 4 (2014):1393-1399, https://doi.org/10.2298/ABS1404393P . .