Pectobacterium carotovorum subsp. carotovorum - The causal agent of broccoli soft rot in Serbia
Pectobacterium carotovorum subsp. carotovorum - prouzrokovač vlažne truleži brokolija u Srbiji
2014
Authors
Gašić, KatarinaGavrilović, Veljko
Dolovac, Nenad
Trkulja, Nenad
Živković, Svetlana
Ristić, Danijela
Obradović, Aleksa
Article (Published version)
Metadata
Show full item recordAbstract
Soft rot symptoms were observed on broccoli plants in several commercial fields in the western part of Serbia. Six strains of bacteria were isolated from diseased tissues and identified as Pectobacterium carotovorum subsp. carotovorum using conventional bacteriological and molecular methods. All strains were non-fluorescent, gram-negative, facultative anaerobes, oxidase-negative and catalase-positive, causing soft rot on potato and carrot slices and did not induce hypersensitive reaction on tobacco leaves. They grew in 5% NaCl and at 37C, did not produce acid from α-methyl glucoside, sorbitol and maltose, nor reducing substances from sucrose, but utilized lactose and trehalose, and did not produce indole or lecithinase. The investigated strains showed characteristic growth on Logan's medium and did not produce blue pigmented indigoidine on GYCA medium nor 'fried egg' colonies on PDA. The identity of strains was confirmed by ITS-PCR and ITS-RFLP analyses and by sequence analysis of the ...16S rRNA gene. In a pathogenicity assay, all strains caused tissue discoloration and soft rot development on inoculated broccoli head tissue fragments.
Simptomi vlažne truleži brokolija uočeni su u nekoliko komercijalnih zasada u zapadnoj Srbiji. Sojevi bakterija izolovani su iz obolelog biljnog tkiva i identifikovani kao Pectobacterium carotovorum subsp. carotovorum korišćenjem konvencionalnih bakterioloških i novijih molekularnih metoda. Svi proučavani sojevi bili su nefluorescentni, Gram-negativni, fakultativno-anaerobni, oksidaza i lecitinaza negativni, katalaza pozitivni, prouzrokujući vlažnu trulež cvetnih drški brokolija, kriški krompira i kriški mrkve, ali nisu indukovali hipersenzitivnu reakciju duvana. Svi sojevi razvijaju se pri 37C i u prisustvu 5% NaCl, ne proizvode kiselinu iz α-metil glukozida, sorbitola i maloze, niti redukujuće supstance iz saharoze, ali razlažu laktozu i trehalozu, i ne stvaraju indol. Proučavani sojevi ispoljili su karakterističan razvoj na Loganovoj diferencijalnoj podlozi, i nisu proizvodili plavi pigment na GYCA podlozi niti 'fried egg' kolonije na PDA podlozi. Identifikacija sojeva potvrđena je ...korišćenjem ITS-PCR i ITS-RFLP, kao i analizom sekvenci 16S rRNA gena. Prilikom izvođenja testa patogenosti, svi sojevi prouzrokovali su obezbojavanje tkiva i vlažnu trulež cvetnih drški brokolija.
Keywords:
Broccoli / Pectobacterium carotovorum subsp. carotovorum / Soft rot / 16S rRNAgene / ITS-PCR-RFLP / Brokoli / Pectobacterium carotovorum subsp. carotovorum / vlažna trulež / 16S rRNA gen / ITS-PCR-RFLPSource:
Pesticidi i fitomedicina, 2014, 29, 4, 249-255Publisher:
- Institute of Pesticides and Environmental Protection
Funding / projects:
- Development of integrated management of harmful organisms in plant production in order to overcome resistance and to improve food quality and safety (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-46008)
- Development of integrated approach in plant protection for control harmful organisms (RS-MESTD-Technological Development (TD or TR)-31018)
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Institution/Community
IZBISTY - JOUR AU - Gašić, Katarina AU - Gavrilović, Veljko AU - Dolovac, Nenad AU - Trkulja, Nenad AU - Živković, Svetlana AU - Ristić, Danijela AU - Obradović, Aleksa PY - 2014 UR - https://plantarum.izbis.bg.ac.rs/handle/123456789/362 AB - Soft rot symptoms were observed on broccoli plants in several commercial fields in the western part of Serbia. Six strains of bacteria were isolated from diseased tissues and identified as Pectobacterium carotovorum subsp. carotovorum using conventional bacteriological and molecular methods. All strains were non-fluorescent, gram-negative, facultative anaerobes, oxidase-negative and catalase-positive, causing soft rot on potato and carrot slices and did not induce hypersensitive reaction on tobacco leaves. They grew in 5% NaCl and at 37C, did not produce acid from α-methyl glucoside, sorbitol and maltose, nor reducing substances from sucrose, but utilized lactose and trehalose, and did not produce indole or lecithinase. The investigated strains showed characteristic growth on Logan's medium and did not produce blue pigmented indigoidine on GYCA medium nor 'fried egg' colonies on PDA. The identity of strains was confirmed by ITS-PCR and ITS-RFLP analyses and by sequence analysis of the 16S rRNA gene. In a pathogenicity assay, all strains caused tissue discoloration and soft rot development on inoculated broccoli head tissue fragments. AB - Simptomi vlažne truleži brokolija uočeni su u nekoliko komercijalnih zasada u zapadnoj Srbiji. Sojevi bakterija izolovani su iz obolelog biljnog tkiva i identifikovani kao Pectobacterium carotovorum subsp. carotovorum korišćenjem konvencionalnih bakterioloških i novijih molekularnih metoda. Svi proučavani sojevi bili su nefluorescentni, Gram-negativni, fakultativno-anaerobni, oksidaza i lecitinaza negativni, katalaza pozitivni, prouzrokujući vlažnu trulež cvetnih drški brokolija, kriški krompira i kriški mrkve, ali nisu indukovali hipersenzitivnu reakciju duvana. Svi sojevi razvijaju se pri 37C i u prisustvu 5% NaCl, ne proizvode kiselinu iz α-metil glukozida, sorbitola i maloze, niti redukujuće supstance iz saharoze, ali razlažu laktozu i trehalozu, i ne stvaraju indol. Proučavani sojevi ispoljili su karakterističan razvoj na Loganovoj diferencijalnoj podlozi, i nisu proizvodili plavi pigment na GYCA podlozi niti 'fried egg' kolonije na PDA podlozi. Identifikacija sojeva potvrđena je korišćenjem ITS-PCR i ITS-RFLP, kao i analizom sekvenci 16S rRNA gena. Prilikom izvođenja testa patogenosti, svi sojevi prouzrokovali su obezbojavanje tkiva i vlažnu trulež cvetnih drški brokolija. PB - Institute of Pesticides and Environmental Protection T2 - Pesticidi i fitomedicina T1 - Pectobacterium carotovorum subsp. carotovorum - The causal agent of broccoli soft rot in Serbia T1 - Pectobacterium carotovorum subsp. carotovorum - prouzrokovač vlažne truleži brokolija u Srbiji EP - 255 IS - 4 SP - 249 VL - 29 DO - 10.2298/PIF1404249G ER -
@article{ author = "Gašić, Katarina and Gavrilović, Veljko and Dolovac, Nenad and Trkulja, Nenad and Živković, Svetlana and Ristić, Danijela and Obradović, Aleksa", year = "2014", abstract = "Soft rot symptoms were observed on broccoli plants in several commercial fields in the western part of Serbia. Six strains of bacteria were isolated from diseased tissues and identified as Pectobacterium carotovorum subsp. carotovorum using conventional bacteriological and molecular methods. All strains were non-fluorescent, gram-negative, facultative anaerobes, oxidase-negative and catalase-positive, causing soft rot on potato and carrot slices and did not induce hypersensitive reaction on tobacco leaves. They grew in 5% NaCl and at 37C, did not produce acid from α-methyl glucoside, sorbitol and maltose, nor reducing substances from sucrose, but utilized lactose and trehalose, and did not produce indole or lecithinase. The investigated strains showed characteristic growth on Logan's medium and did not produce blue pigmented indigoidine on GYCA medium nor 'fried egg' colonies on PDA. The identity of strains was confirmed by ITS-PCR and ITS-RFLP analyses and by sequence analysis of the 16S rRNA gene. In a pathogenicity assay, all strains caused tissue discoloration and soft rot development on inoculated broccoli head tissue fragments., Simptomi vlažne truleži brokolija uočeni su u nekoliko komercijalnih zasada u zapadnoj Srbiji. Sojevi bakterija izolovani su iz obolelog biljnog tkiva i identifikovani kao Pectobacterium carotovorum subsp. carotovorum korišćenjem konvencionalnih bakterioloških i novijih molekularnih metoda. Svi proučavani sojevi bili su nefluorescentni, Gram-negativni, fakultativno-anaerobni, oksidaza i lecitinaza negativni, katalaza pozitivni, prouzrokujući vlažnu trulež cvetnih drški brokolija, kriški krompira i kriški mrkve, ali nisu indukovali hipersenzitivnu reakciju duvana. Svi sojevi razvijaju se pri 37C i u prisustvu 5% NaCl, ne proizvode kiselinu iz α-metil glukozida, sorbitola i maloze, niti redukujuće supstance iz saharoze, ali razlažu laktozu i trehalozu, i ne stvaraju indol. Proučavani sojevi ispoljili su karakterističan razvoj na Loganovoj diferencijalnoj podlozi, i nisu proizvodili plavi pigment na GYCA podlozi niti 'fried egg' kolonije na PDA podlozi. Identifikacija sojeva potvrđena je korišćenjem ITS-PCR i ITS-RFLP, kao i analizom sekvenci 16S rRNA gena. Prilikom izvođenja testa patogenosti, svi sojevi prouzrokovali su obezbojavanje tkiva i vlažnu trulež cvetnih drški brokolija.", publisher = "Institute of Pesticides and Environmental Protection", journal = "Pesticidi i fitomedicina", title = "Pectobacterium carotovorum subsp. carotovorum - The causal agent of broccoli soft rot in Serbia, Pectobacterium carotovorum subsp. carotovorum - prouzrokovač vlažne truleži brokolija u Srbiji", pages = "255-249", number = "4", volume = "29", doi = "10.2298/PIF1404249G" }
Gašić, K., Gavrilović, V., Dolovac, N., Trkulja, N., Živković, S., Ristić, D.,& Obradović, A.. (2014). Pectobacterium carotovorum subsp. carotovorum - The causal agent of broccoli soft rot in Serbia. in Pesticidi i fitomedicina Institute of Pesticides and Environmental Protection., 29(4), 249-255. https://doi.org/10.2298/PIF1404249G
Gašić K, Gavrilović V, Dolovac N, Trkulja N, Živković S, Ristić D, Obradović A. Pectobacterium carotovorum subsp. carotovorum - The causal agent of broccoli soft rot in Serbia. in Pesticidi i fitomedicina. 2014;29(4):249-255. doi:10.2298/PIF1404249G .
Gašić, Katarina, Gavrilović, Veljko, Dolovac, Nenad, Trkulja, Nenad, Živković, Svetlana, Ristić, Danijela, Obradović, Aleksa, "Pectobacterium carotovorum subsp. carotovorum - The causal agent of broccoli soft rot in Serbia" in Pesticidi i fitomedicina, 29, no. 4 (2014):249-255, https://doi.org/10.2298/PIF1404249G . .
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