Molecular assessment of genetic diversity of Xanthomonas arboricola pv. juglandis strains from Serbia by various DNA fingerprinting techniques
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2015
Authors
Ivanović, ŽarkoPopović, Tatjana
Janse, Jaap
Kojić, Milan
Stanković, Slaviša
Gavrilović, Veljko
Fira, Đorđe
Article (Published version)
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The purpose of the present study was to investigate the genetic diversity of X. arboricola pv. juglandis strains in Serbia. This bacterium is the causal agent of walnut blight and is also associated with apical necrosis of immature walnut fruits. Although walnut blight is long known and widespread in Serbia, a systematic strain diversity study of Xanthomonas arboricola pv. juglandis strains from different regions in Serbia has not been performed. The objectives of this work were to examine the molecular diversity and its possible biological significance of 59 isolates of X. arboricola pv. juglandis collected from different geographic locations in Serbia. Genomic variability was assessed by using repetitive PCR, SpeI macrorestriction analysis of genomic DNAs by pulsed-field gel electrophoresis (PFGE) and partial sequencing of the gyrB gene. Molecular analyses showed substantial genetic diversity among strains and existence of diverse populations of X. arboricola pv. juglandis in Serbia.
Keywords:
Xanthomonas arboricola pv. juglandis / Walnut / CharacterizationSource:
European Journal of Plant Pathology, 2015, 141, 1, 133-145Publisher:
- Springer, Dordrecht
Funding / projects:
- Molecular characterization of bacteria from genera Bacillus and Pseudomonas as potential agents for biological control (RS-173026)
- Development of integrated approach in plant protection for control harmful organisms (RS-31018)
DOI: 10.1007/s10658-014-0531-5
ISSN: 0929-1873
WoS: 000347303300013
Scopus: 2-s2.0-84920587798
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IZBISTY - JOUR AU - Ivanović, Žarko AU - Popović, Tatjana AU - Janse, Jaap AU - Kojić, Milan AU - Stanković, Slaviša AU - Gavrilović, Veljko AU - Fira, Đorđe PY - 2015 UR - https://plantarum.izbis.bg.ac.rs/handle/123456789/396 AB - The purpose of the present study was to investigate the genetic diversity of X. arboricola pv. juglandis strains in Serbia. This bacterium is the causal agent of walnut blight and is also associated with apical necrosis of immature walnut fruits. Although walnut blight is long known and widespread in Serbia, a systematic strain diversity study of Xanthomonas arboricola pv. juglandis strains from different regions in Serbia has not been performed. The objectives of this work were to examine the molecular diversity and its possible biological significance of 59 isolates of X. arboricola pv. juglandis collected from different geographic locations in Serbia. Genomic variability was assessed by using repetitive PCR, SpeI macrorestriction analysis of genomic DNAs by pulsed-field gel electrophoresis (PFGE) and partial sequencing of the gyrB gene. Molecular analyses showed substantial genetic diversity among strains and existence of diverse populations of X. arboricola pv. juglandis in Serbia. PB - Springer, Dordrecht T2 - European Journal of Plant Pathology T1 - Molecular assessment of genetic diversity of Xanthomonas arboricola pv. juglandis strains from Serbia by various DNA fingerprinting techniques EP - 145 IS - 1 SP - 133 VL - 141 DO - 10.1007/s10658-014-0531-5 ER -
@article{ author = "Ivanović, Žarko and Popović, Tatjana and Janse, Jaap and Kojić, Milan and Stanković, Slaviša and Gavrilović, Veljko and Fira, Đorđe", year = "2015", abstract = "The purpose of the present study was to investigate the genetic diversity of X. arboricola pv. juglandis strains in Serbia. This bacterium is the causal agent of walnut blight and is also associated with apical necrosis of immature walnut fruits. Although walnut blight is long known and widespread in Serbia, a systematic strain diversity study of Xanthomonas arboricola pv. juglandis strains from different regions in Serbia has not been performed. The objectives of this work were to examine the molecular diversity and its possible biological significance of 59 isolates of X. arboricola pv. juglandis collected from different geographic locations in Serbia. Genomic variability was assessed by using repetitive PCR, SpeI macrorestriction analysis of genomic DNAs by pulsed-field gel electrophoresis (PFGE) and partial sequencing of the gyrB gene. Molecular analyses showed substantial genetic diversity among strains and existence of diverse populations of X. arboricola pv. juglandis in Serbia.", publisher = "Springer, Dordrecht", journal = "European Journal of Plant Pathology", title = "Molecular assessment of genetic diversity of Xanthomonas arboricola pv. juglandis strains from Serbia by various DNA fingerprinting techniques", pages = "145-133", number = "1", volume = "141", doi = "10.1007/s10658-014-0531-5" }
Ivanović, Ž., Popović, T., Janse, J., Kojić, M., Stanković, S., Gavrilović, V.,& Fira, Đ.. (2015). Molecular assessment of genetic diversity of Xanthomonas arboricola pv. juglandis strains from Serbia by various DNA fingerprinting techniques. in European Journal of Plant Pathology Springer, Dordrecht., 141(1), 133-145. https://doi.org/10.1007/s10658-014-0531-5
Ivanović Ž, Popović T, Janse J, Kojić M, Stanković S, Gavrilović V, Fira Đ. Molecular assessment of genetic diversity of Xanthomonas arboricola pv. juglandis strains from Serbia by various DNA fingerprinting techniques. in European Journal of Plant Pathology. 2015;141(1):133-145. doi:10.1007/s10658-014-0531-5 .
Ivanović, Žarko, Popović, Tatjana, Janse, Jaap, Kojić, Milan, Stanković, Slaviša, Gavrilović, Veljko, Fira, Đorđe, "Molecular assessment of genetic diversity of Xanthomonas arboricola pv. juglandis strains from Serbia by various DNA fingerprinting techniques" in European Journal of Plant Pathology, 141, no. 1 (2015):133-145, https://doi.org/10.1007/s10658-014-0531-5 . .