Identification and characterization of Dickeya zeae strains associated with maize stalk soft-rot in northern Serbia
Само за регистроване кориснике
2020
Аутори
Prokić, AndjelkaZlatković, Nevena
Kuzmanović, Nemanja
Ivanović, Milan
Gašić, K.
Pavlović, Z.
Obradović, Aleksa
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Bacterial stalk soft rot have been repeatedly observed on maize plants in several commercial fields in northern part of Serbia in the period of 1990 to 2014. The occurrence of the disease corresponded with warm weather and increased humidity. Etiological studies of the diseased tissue constantly resulted in isolation of pectolytic bacterial strains. In order to identify the isolated bacteria, twenty-three strains were characterized by morphological, physiolcal, biochemical, and molecular assays. Pathogenicity of the strains was confirmed by Koch's postulates on 1-week-old maize seedlings. The strains were Gram and oxidase-negative, non-fluorescent, pectolytic, facultative anaerobic and caused hypersensitive response (HR) in tobacco leaves. They produced catalase and lecithinase, but did not produce oxidase and arginine dehydrolase. All strains reduced nitrate and grew at 37 degrees C, while variable growth was observed in medium containing 5% NaCl. Phenotypic tests and amplification of... the specific 420-bp fragment in PCR assay showed that the strains belong to genus Dickeya. Using ERIC-PCR analysis seven different genetic profiles were obtained, suggesting the presence of genetic diversity in the population of this pathogen in Serbia. Additionally, phylogenetic analysis based on the recA gene sequence analysis indicated that the strains isolated from soft rotted maize belong to Dickeya zeae, leading to the conclusion that this bacterium was the causal agent of stalk soft rot of maize in Serbia.
Кључне речи:
Dickeya zeae / Maize / Bacterial stalk soft rot / Phenotypic tests / ERIC-PCR / recA gene sequence analysisИзвор:
European Journal of Plant Pathology, 2020, 157, 3, 685-691Издавач:
- Springer, Dordrecht
Финансирање / пројекти:
- Развој интегрисаних система управљања штетним организмима у биљној производњи са циљем превазилажења резистентности и унапређења квалитета и безбедности хране (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-46008)
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200116 (Универзитет у Београду, Пољопривредни факултет) (RS-MESTD-inst-2020-200116)
DOI: 10.1007/s10658-020-02019-4
ISSN: 0929-1873
WoS: 000536070000001
Scopus: 2-s2.0-85085596479
Институција/група
IZBISTY - JOUR AU - Prokić, Andjelka AU - Zlatković, Nevena AU - Kuzmanović, Nemanja AU - Ivanović, Milan AU - Gašić, K. AU - Pavlović, Z. AU - Obradović, Aleksa PY - 2020 UR - https://plantarum.izbis.bg.ac.rs/handle/123456789/592 AB - Bacterial stalk soft rot have been repeatedly observed on maize plants in several commercial fields in northern part of Serbia in the period of 1990 to 2014. The occurrence of the disease corresponded with warm weather and increased humidity. Etiological studies of the diseased tissue constantly resulted in isolation of pectolytic bacterial strains. In order to identify the isolated bacteria, twenty-three strains were characterized by morphological, physiolcal, biochemical, and molecular assays. Pathogenicity of the strains was confirmed by Koch's postulates on 1-week-old maize seedlings. The strains were Gram and oxidase-negative, non-fluorescent, pectolytic, facultative anaerobic and caused hypersensitive response (HR) in tobacco leaves. They produced catalase and lecithinase, but did not produce oxidase and arginine dehydrolase. All strains reduced nitrate and grew at 37 degrees C, while variable growth was observed in medium containing 5% NaCl. Phenotypic tests and amplification of the specific 420-bp fragment in PCR assay showed that the strains belong to genus Dickeya. Using ERIC-PCR analysis seven different genetic profiles were obtained, suggesting the presence of genetic diversity in the population of this pathogen in Serbia. Additionally, phylogenetic analysis based on the recA gene sequence analysis indicated that the strains isolated from soft rotted maize belong to Dickeya zeae, leading to the conclusion that this bacterium was the causal agent of stalk soft rot of maize in Serbia. PB - Springer, Dordrecht T2 - European Journal of Plant Pathology T1 - Identification and characterization of Dickeya zeae strains associated with maize stalk soft-rot in northern Serbia EP - 691 IS - 3 SP - 685 VL - 157 DO - 10.1007/s10658-020-02019-4 ER -
@article{ author = "Prokić, Andjelka and Zlatković, Nevena and Kuzmanović, Nemanja and Ivanović, Milan and Gašić, K. and Pavlović, Z. and Obradović, Aleksa", year = "2020", abstract = "Bacterial stalk soft rot have been repeatedly observed on maize plants in several commercial fields in northern part of Serbia in the period of 1990 to 2014. The occurrence of the disease corresponded with warm weather and increased humidity. Etiological studies of the diseased tissue constantly resulted in isolation of pectolytic bacterial strains. In order to identify the isolated bacteria, twenty-three strains were characterized by morphological, physiolcal, biochemical, and molecular assays. Pathogenicity of the strains was confirmed by Koch's postulates on 1-week-old maize seedlings. The strains were Gram and oxidase-negative, non-fluorescent, pectolytic, facultative anaerobic and caused hypersensitive response (HR) in tobacco leaves. They produced catalase and lecithinase, but did not produce oxidase and arginine dehydrolase. All strains reduced nitrate and grew at 37 degrees C, while variable growth was observed in medium containing 5% NaCl. Phenotypic tests and amplification of the specific 420-bp fragment in PCR assay showed that the strains belong to genus Dickeya. Using ERIC-PCR analysis seven different genetic profiles were obtained, suggesting the presence of genetic diversity in the population of this pathogen in Serbia. Additionally, phylogenetic analysis based on the recA gene sequence analysis indicated that the strains isolated from soft rotted maize belong to Dickeya zeae, leading to the conclusion that this bacterium was the causal agent of stalk soft rot of maize in Serbia.", publisher = "Springer, Dordrecht", journal = "European Journal of Plant Pathology", title = "Identification and characterization of Dickeya zeae strains associated with maize stalk soft-rot in northern Serbia", pages = "691-685", number = "3", volume = "157", doi = "10.1007/s10658-020-02019-4" }
Prokić, A., Zlatković, N., Kuzmanović, N., Ivanović, M., Gašić, K., Pavlović, Z.,& Obradović, A.. (2020). Identification and characterization of Dickeya zeae strains associated with maize stalk soft-rot in northern Serbia. in European Journal of Plant Pathology Springer, Dordrecht., 157(3), 685-691. https://doi.org/10.1007/s10658-020-02019-4
Prokić A, Zlatković N, Kuzmanović N, Ivanović M, Gašić K, Pavlović Z, Obradović A. Identification and characterization of Dickeya zeae strains associated with maize stalk soft-rot in northern Serbia. in European Journal of Plant Pathology. 2020;157(3):685-691. doi:10.1007/s10658-020-02019-4 .
Prokić, Andjelka, Zlatković, Nevena, Kuzmanović, Nemanja, Ivanović, Milan, Gašić, K., Pavlović, Z., Obradović, Aleksa, "Identification and characterization of Dickeya zeae strains associated with maize stalk soft-rot in northern Serbia" in European Journal of Plant Pathology, 157, no. 3 (2020):685-691, https://doi.org/10.1007/s10658-020-02019-4 . .