Development and validation of LC-MS/MS method for the citrinin determination in red rice

Abstract

The liquid chromatography tandem mass spectrometry (LC-MS/MS) method for the citrinin (CIN) determination in red rice was developed. The mycotoxin was extracted from red rice using anetonitrile/water/acetic acid mixture followed by the clean-up step on Captiva EMR cartridges. The developed method was validated according to the Commission Regulation No. 401/2006/EC. The validation data were evaluated in terms of recoveries, reproducibility, limits of quantification (LOQ), limit of detection (LOD), specificity, linearity and matrix effects for CIN in matrix using 13C20-OTA as an internal standard. The obtained method performance parameters indicate that the method is suitable for the CIN routine analysis.