Elaboration of methods for detection of Xanthomonas axonopodis pv. phaseoli on bean seeds
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method of the International Seed Federation - ISF (2006) was used. It included bacteria extraction from seeds and isolation on semiselective media with pathogenicity test of the investigated isolates. ELISA and PCR were used for verification of results. The results showed that the semiselective media MT (Milk Tween Agar) and XCP1 (Xanthomonas campestris pv. phaseoli Agar) were very suitable for isolation of X. a. pv. phaseoli. Pathogenicity was confirmed on young bean plants. ELISA test and PCR confirmed that all investigated isolates and reisolates belong to the bacterium X.a.pv. phaseoli.
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Xanthomonas axonopodis pv. phaseoli / bean / seed / artificial inoculation / ELISA / PCRSource:
Pesticidi i fitomedicina, 2008, 23, 2, 89-98Publisher:
- Institute of Pesticides and Environmental Protection, Belgrade & Plant Protection Society of Serbia, Belgrade
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IZBISTY - JOUR AU - Balaž, Jelica AU - Popović Milovanović, Tatjana AU - Vasić, Mirjana AU - Nikolić, Zorica PY - 2008 UR - https://plantarum.izbis.bg.ac.rs/handle/123456789/1063 AB - method of the International Seed Federation - ISF (2006) was used. It included bacteria extraction from seeds and isolation on semiselective media with pathogenicity test of the investigated isolates. ELISA and PCR were used for verification of results. The results showed that the semiselective media MT (Milk Tween Agar) and XCP1 (Xanthomonas campestris pv. phaseoli Agar) were very suitable for isolation of X. a. pv. phaseoli. Pathogenicity was confirmed on young bean plants. ELISA test and PCR confirmed that all investigated isolates and reisolates belong to the bacterium X.a.pv. phaseoli. PB - Institute of Pesticides and Environmental Protection, Belgrade & Plant Protection Society of Serbia, Belgrade T2 - Pesticidi i fitomedicina T1 - Elaboration of methods for detection of Xanthomonas axonopodis pv. phaseoli on bean seeds EP - 98 IS - 2 SP - 89 VL - 23 DO - 10.2298/PIF0802089B ER -
@article{ author = "Balaž, Jelica and Popović Milovanović, Tatjana and Vasić, Mirjana and Nikolić, Zorica", year = "2008", abstract = "method of the International Seed Federation - ISF (2006) was used. It included bacteria extraction from seeds and isolation on semiselective media with pathogenicity test of the investigated isolates. ELISA and PCR were used for verification of results. The results showed that the semiselective media MT (Milk Tween Agar) and XCP1 (Xanthomonas campestris pv. phaseoli Agar) were very suitable for isolation of X. a. pv. phaseoli. Pathogenicity was confirmed on young bean plants. ELISA test and PCR confirmed that all investigated isolates and reisolates belong to the bacterium X.a.pv. phaseoli.", publisher = "Institute of Pesticides and Environmental Protection, Belgrade & Plant Protection Society of Serbia, Belgrade", journal = "Pesticidi i fitomedicina", title = "Elaboration of methods for detection of Xanthomonas axonopodis pv. phaseoli on bean seeds", pages = "98-89", number = "2", volume = "23", doi = "10.2298/PIF0802089B" }
Balaž, J., Popović Milovanović, T., Vasić, M.,& Nikolić, Z.. (2008). Elaboration of methods for detection of Xanthomonas axonopodis pv. phaseoli on bean seeds. in Pesticidi i fitomedicina Institute of Pesticides and Environmental Protection, Belgrade & Plant Protection Society of Serbia, Belgrade., 23(2), 89-98. https://doi.org/10.2298/PIF0802089B
Balaž J, Popović Milovanović T, Vasić M, Nikolić Z. Elaboration of methods for detection of Xanthomonas axonopodis pv. phaseoli on bean seeds. in Pesticidi i fitomedicina. 2008;23(2):89-98. doi:10.2298/PIF0802089B .
Balaž, Jelica, Popović Milovanović, Tatjana, Vasić, Mirjana, Nikolić, Zorica, "Elaboration of methods for detection of Xanthomonas axonopodis pv. phaseoli on bean seeds" in Pesticidi i fitomedicina, 23, no. 2 (2008):89-98, https://doi.org/10.2298/PIF0802089B . .