Incidence and molecular characterization of flavescence doree and stolbur phytoplasmas in grapevine cultivars from different viticultural areas of Serbia
Nema prikaza
Autori
Kuzmanović, SlobodanMartini, M.
Ermacora, P.
Ferrini, F.
Starović, Mira
Tošić, Mališa
Carraro, L.
Osler, R.
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
The presence and distribution of grapevine phytoplasmas was investigated from 2003 to 2005 in some of the most important viticultural areas of Serbia, considering in particular the susceptibility and sensitiveness of both local and imported grapevine cultivars. Both flavescence doree (FD) and bois noir (BN) phytoplasmas were detected using molecular techniques. The presence of FD phytoplasma at the moment seems limited, while BN phytoplasma appears to be present in the majority of grape growing regions in Serbia. Field surveys demonstrate that grapevine yellows (GY) epidemics in the vineyards inspected in Serbia spread very fast, indeed the incidence of symptomatic plants increased considerably year by year. In particular, the average rate of FD diffusion increased from 45.5 to 93.0 % in the Sicevacko region, while the spread of BN resulted lower. The local cultivar 'Plovdina' appeared to be extremely sensitive to FD phytoplasma showing a percentage of infected plants ranging from 91 t...o 100 %. PCR-RFLP and phylogenetic analyses based on ribosomal protein (rp) and secY gene sequences performed on Serbian FD grapevine strains demonstrated their close relationship with the Italian FD-C strain present in north-east Italy. Based on both phylogenetic markers, Serbian FD strains represent a new distinct lineage and together with the FD-C strain form a major phylogenetic group within the elm yellows group.
Ključne reči:
grapevine yellows / PCR/RFLP / phylogenetic analysis / ribosomal protein / secYIzvor:
VITIS, 2008, 47, 2, 105-111Izdavač:
- Jki-Institut Rebenzuchtung, Siebeldingen
Institucija/grupa
IZBISTY - JOUR AU - Kuzmanović, Slobodan AU - Martini, M. AU - Ermacora, P. AU - Ferrini, F. AU - Starović, Mira AU - Tošić, Mališa AU - Carraro, L. AU - Osler, R. PY - 2008 UR - https://plantarum.izbis.bg.ac.rs/handle/123456789/72 AB - The presence and distribution of grapevine phytoplasmas was investigated from 2003 to 2005 in some of the most important viticultural areas of Serbia, considering in particular the susceptibility and sensitiveness of both local and imported grapevine cultivars. Both flavescence doree (FD) and bois noir (BN) phytoplasmas were detected using molecular techniques. The presence of FD phytoplasma at the moment seems limited, while BN phytoplasma appears to be present in the majority of grape growing regions in Serbia. Field surveys demonstrate that grapevine yellows (GY) epidemics in the vineyards inspected in Serbia spread very fast, indeed the incidence of symptomatic plants increased considerably year by year. In particular, the average rate of FD diffusion increased from 45.5 to 93.0 % in the Sicevacko region, while the spread of BN resulted lower. The local cultivar 'Plovdina' appeared to be extremely sensitive to FD phytoplasma showing a percentage of infected plants ranging from 91 to 100 %. PCR-RFLP and phylogenetic analyses based on ribosomal protein (rp) and secY gene sequences performed on Serbian FD grapevine strains demonstrated their close relationship with the Italian FD-C strain present in north-east Italy. Based on both phylogenetic markers, Serbian FD strains represent a new distinct lineage and together with the FD-C strain form a major phylogenetic group within the elm yellows group. PB - Jki-Institut Rebenzuchtung, Siebeldingen T2 - VITIS T1 - Incidence and molecular characterization of flavescence doree and stolbur phytoplasmas in grapevine cultivars from different viticultural areas of Serbia EP - 111 IS - 2 SP - 105 VL - 47 ER -
@article{ author = "Kuzmanović, Slobodan and Martini, M. and Ermacora, P. and Ferrini, F. and Starović, Mira and Tošić, Mališa and Carraro, L. and Osler, R.", year = "2008", abstract = "The presence and distribution of grapevine phytoplasmas was investigated from 2003 to 2005 in some of the most important viticultural areas of Serbia, considering in particular the susceptibility and sensitiveness of both local and imported grapevine cultivars. Both flavescence doree (FD) and bois noir (BN) phytoplasmas were detected using molecular techniques. The presence of FD phytoplasma at the moment seems limited, while BN phytoplasma appears to be present in the majority of grape growing regions in Serbia. Field surveys demonstrate that grapevine yellows (GY) epidemics in the vineyards inspected in Serbia spread very fast, indeed the incidence of symptomatic plants increased considerably year by year. In particular, the average rate of FD diffusion increased from 45.5 to 93.0 % in the Sicevacko region, while the spread of BN resulted lower. The local cultivar 'Plovdina' appeared to be extremely sensitive to FD phytoplasma showing a percentage of infected plants ranging from 91 to 100 %. PCR-RFLP and phylogenetic analyses based on ribosomal protein (rp) and secY gene sequences performed on Serbian FD grapevine strains demonstrated their close relationship with the Italian FD-C strain present in north-east Italy. Based on both phylogenetic markers, Serbian FD strains represent a new distinct lineage and together with the FD-C strain form a major phylogenetic group within the elm yellows group.", publisher = "Jki-Institut Rebenzuchtung, Siebeldingen", journal = "VITIS", title = "Incidence and molecular characterization of flavescence doree and stolbur phytoplasmas in grapevine cultivars from different viticultural areas of Serbia", pages = "111-105", number = "2", volume = "47" }
Kuzmanović, S., Martini, M., Ermacora, P., Ferrini, F., Starović, M., Tošić, M., Carraro, L.,& Osler, R.. (2008). Incidence and molecular characterization of flavescence doree and stolbur phytoplasmas in grapevine cultivars from different viticultural areas of Serbia. in VITIS Jki-Institut Rebenzuchtung, Siebeldingen., 47(2), 105-111.
Kuzmanović S, Martini M, Ermacora P, Ferrini F, Starović M, Tošić M, Carraro L, Osler R. Incidence and molecular characterization of flavescence doree and stolbur phytoplasmas in grapevine cultivars from different viticultural areas of Serbia. in VITIS. 2008;47(2):105-111..
Kuzmanović, Slobodan, Martini, M., Ermacora, P., Ferrini, F., Starović, Mira, Tošić, Mališa, Carraro, L., Osler, R., "Incidence and molecular characterization of flavescence doree and stolbur phytoplasmas in grapevine cultivars from different viticultural areas of Serbia" in VITIS, 47, no. 2 (2008):105-111.