In vitro and in vivo antifungal properties of cysteine proteinase inhibitor from green kiwifruit
Nema prikaza
Autori
Popović, MilicaBulajić, Aleksandra
Ristić, Danijela
Krstić, Branka
Jankov, Ratko
Gavrilović-Jankulović, Marija
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
Higher plants possess several mechanisms of defense against plant pathogens. Proteins actively synthesized in response to those stresses are called defense-related proteins which, among others, include certain protease inhibitors. It is of particular relevance to investigate plant natural defense mechanisms for pathogen control which include cystatinsspecific inhibitors of cysteine proteases. RESULTS: In this study, a cysteine proteinase inhibitor (CPI), 11 kDa in size, was purified from green kiwifruit to homogeneity. Immuno-tissue print results indicated that CPI is most abundant in the outer layer of pericarp, near the peel, and the inner most part of the pulpsites where it could act as a natural barrier against pathogens entering the fruit. The purified protein (15 mu mol L-1) showed antifungal activity against two phytopathogenic fungi (Alternaria radicina and Botrytis cinerea) by inhibiting fungal spore germination. In vivo, CPI (10 mu mol L-1) was able to prevent artificial infe...ction of apple and carrot with spore suspension of B. cinerea and A. radicina, respectively. It also exerted activity on both intracellular and fermentation fluid proteinases. CONCLUSION: Identification and characterization of plant defense molecules is the first step towards creation of improved methods for pathogen control based on naturally occurring molecules.
Ključne reči:
cysteine protease inhibitor / kiwifruit cystatin / antifungal / plant natural defense / Alternaria radicina / Botrytis cinereaIzvor:
Journal of the Science of Food and Agriculture, 2012, 92, 15, 3072-3078Izdavač:
- Wiley
Finansiranje / projekti:
- Alergeni, antitela, enzimi i mali fiziološki značajni molekuli: dizajn, struktura, funkcija i značaj (RS-MESTD-Basic Research (BR or ON)-172049)
- Identifikacija i karakterizacija ćelijskih kofaktora HIV-a i njihova moguća primena u preventivi i terapiji (RS-MESTD-MPN2006-2010-143001)
DOI: 10.1002/jsfa.5728
ISSN: 0022-5142
PubMed: 22653546
WoS: 000310249600019
Scopus: 2-s2.0-84867900945
Institucija/grupa
IZBISTY - JOUR AU - Popović, Milica AU - Bulajić, Aleksandra AU - Ristić, Danijela AU - Krstić, Branka AU - Jankov, Ratko AU - Gavrilović-Jankulović, Marija PY - 2012 UR - https://plantarum.izbis.bg.ac.rs/handle/123456789/933 AB - Higher plants possess several mechanisms of defense against plant pathogens. Proteins actively synthesized in response to those stresses are called defense-related proteins which, among others, include certain protease inhibitors. It is of particular relevance to investigate plant natural defense mechanisms for pathogen control which include cystatinsspecific inhibitors of cysteine proteases. RESULTS: In this study, a cysteine proteinase inhibitor (CPI), 11 kDa in size, was purified from green kiwifruit to homogeneity. Immuno-tissue print results indicated that CPI is most abundant in the outer layer of pericarp, near the peel, and the inner most part of the pulpsites where it could act as a natural barrier against pathogens entering the fruit. The purified protein (15 mu mol L-1) showed antifungal activity against two phytopathogenic fungi (Alternaria radicina and Botrytis cinerea) by inhibiting fungal spore germination. In vivo, CPI (10 mu mol L-1) was able to prevent artificial infection of apple and carrot with spore suspension of B. cinerea and A. radicina, respectively. It also exerted activity on both intracellular and fermentation fluid proteinases. CONCLUSION: Identification and characterization of plant defense molecules is the first step towards creation of improved methods for pathogen control based on naturally occurring molecules. PB - Wiley T2 - Journal of the Science of Food and Agriculture T1 - In vitro and in vivo antifungal properties of cysteine proteinase inhibitor from green kiwifruit EP - 3078 IS - 15 SP - 3072 VL - 92 DO - 10.1002/jsfa.5728 ER -
@article{ author = "Popović, Milica and Bulajić, Aleksandra and Ristić, Danijela and Krstić, Branka and Jankov, Ratko and Gavrilović-Jankulović, Marija", year = "2012", abstract = "Higher plants possess several mechanisms of defense against plant pathogens. Proteins actively synthesized in response to those stresses are called defense-related proteins which, among others, include certain protease inhibitors. It is of particular relevance to investigate plant natural defense mechanisms for pathogen control which include cystatinsspecific inhibitors of cysteine proteases. RESULTS: In this study, a cysteine proteinase inhibitor (CPI), 11 kDa in size, was purified from green kiwifruit to homogeneity. Immuno-tissue print results indicated that CPI is most abundant in the outer layer of pericarp, near the peel, and the inner most part of the pulpsites where it could act as a natural barrier against pathogens entering the fruit. The purified protein (15 mu mol L-1) showed antifungal activity against two phytopathogenic fungi (Alternaria radicina and Botrytis cinerea) by inhibiting fungal spore germination. In vivo, CPI (10 mu mol L-1) was able to prevent artificial infection of apple and carrot with spore suspension of B. cinerea and A. radicina, respectively. It also exerted activity on both intracellular and fermentation fluid proteinases. CONCLUSION: Identification and characterization of plant defense molecules is the first step towards creation of improved methods for pathogen control based on naturally occurring molecules.", publisher = "Wiley", journal = "Journal of the Science of Food and Agriculture", title = "In vitro and in vivo antifungal properties of cysteine proteinase inhibitor from green kiwifruit", pages = "3078-3072", number = "15", volume = "92", doi = "10.1002/jsfa.5728" }
Popović, M., Bulajić, A., Ristić, D., Krstić, B., Jankov, R.,& Gavrilović-Jankulović, M.. (2012). In vitro and in vivo antifungal properties of cysteine proteinase inhibitor from green kiwifruit. in Journal of the Science of Food and Agriculture Wiley., 92(15), 3072-3078. https://doi.org/10.1002/jsfa.5728
Popović M, Bulajić A, Ristić D, Krstić B, Jankov R, Gavrilović-Jankulović M. In vitro and in vivo antifungal properties of cysteine proteinase inhibitor from green kiwifruit. in Journal of the Science of Food and Agriculture. 2012;92(15):3072-3078. doi:10.1002/jsfa.5728 .
Popović, Milica, Bulajić, Aleksandra, Ristić, Danijela, Krstić, Branka, Jankov, Ratko, Gavrilović-Jankulović, Marija, "In vitro and in vivo antifungal properties of cysteine proteinase inhibitor from green kiwifruit" in Journal of the Science of Food and Agriculture, 92, no. 15 (2012):3072-3078, https://doi.org/10.1002/jsfa.5728 . .