Morphological and molecular identification of Fusarium tricinctum and Fusarium acuminatum as causal agents of garlic bulbs rot in Serbia
Аутори
Ignjatov, MajaBjelić, Dragana
Nikolić, Zorica
Milošević, Dragana
Marinković, Jelena
Ivanović, Žarko
Gvozdanović-Varga, Jelica
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Garlic (Allium sativum L.) is considered to be one of the oldest crops in the world. During 2016, infected garlic bulbs occurred in storages on several localities of the Province of Vojvodina. Symptomatic cloves showed typical rot symptoms such as softened and spongy areas covered with white fungal growth with deep lesions formed on the cloves which became dry over time. A total of 36 isolates of Fusarium species were obtained from diseased cloves of garlic. Colony morphology and microscopic properties of isolated Fusarium species were recorded from the cultures grown on PDA and CLA, respectively. Identification of two chosen isolates was performed by sequencing the EF-1α gene. The TEF sequence of isolate JBL12 showed 100% similarity with several F. tricinctum sequences and sequence of JBL539 showed 99% identity with several F. acuminatum sequences and they were deposited in the NCBI GenBank. Based on the results of the morphological and molecular identification, isolates JBL12 and JBL...539 were identified as F. tricinctum and F. acuminatum, respectively, as new causal agents of garlic bulbs rot in Serbia. Specific primers were designed for the PCR identification of the F. tricinctum.
Кључне речи:
garlic (Allium sativum) / bulb and clove rot / Fusarium spp. / EF-1α geneИзвор:
Zbornik Matice srpske za prirodne nauke, 2017, 133, 271-277Издавач:
- Matica Srpska
Финансирање / пројекти:
- Стварање сората и хибрида поврћа за гајење на отвореном пољу и у заштићеном простору (RS-MESTD-Technological Development (TD or TR)-31030)
Институција/група
IZBISTY - JOUR AU - Ignjatov, Maja AU - Bjelić, Dragana AU - Nikolić, Zorica AU - Milošević, Dragana AU - Marinković, Jelena AU - Ivanović, Žarko AU - Gvozdanović-Varga, Jelica PY - 2017 UR - https://plantarum.izbis.bg.ac.rs/handle/123456789/994 AB - Garlic (Allium sativum L.) is considered to be one of the oldest crops in the world. During 2016, infected garlic bulbs occurred in storages on several localities of the Province of Vojvodina. Symptomatic cloves showed typical rot symptoms such as softened and spongy areas covered with white fungal growth with deep lesions formed on the cloves which became dry over time. A total of 36 isolates of Fusarium species were obtained from diseased cloves of garlic. Colony morphology and microscopic properties of isolated Fusarium species were recorded from the cultures grown on PDA and CLA, respectively. Identification of two chosen isolates was performed by sequencing the EF-1α gene. The TEF sequence of isolate JBL12 showed 100% similarity with several F. tricinctum sequences and sequence of JBL539 showed 99% identity with several F. acuminatum sequences and they were deposited in the NCBI GenBank. Based on the results of the morphological and molecular identification, isolates JBL12 and JBL539 were identified as F. tricinctum and F. acuminatum, respectively, as new causal agents of garlic bulbs rot in Serbia. Specific primers were designed for the PCR identification of the F. tricinctum. PB - Matica Srpska T2 - Zbornik Matice srpske za prirodne nauke T1 - Morphological and molecular identification of Fusarium tricinctum and Fusarium acuminatum as causal agents of garlic bulbs rot in Serbia EP - 277 SP - 271 VL - 133 DO - 10.2298/ZMSPN1733271I ER -
@article{ author = "Ignjatov, Maja and Bjelić, Dragana and Nikolić, Zorica and Milošević, Dragana and Marinković, Jelena and Ivanović, Žarko and Gvozdanović-Varga, Jelica", year = "2017", abstract = "Garlic (Allium sativum L.) is considered to be one of the oldest crops in the world. During 2016, infected garlic bulbs occurred in storages on several localities of the Province of Vojvodina. Symptomatic cloves showed typical rot symptoms such as softened and spongy areas covered with white fungal growth with deep lesions formed on the cloves which became dry over time. A total of 36 isolates of Fusarium species were obtained from diseased cloves of garlic. Colony morphology and microscopic properties of isolated Fusarium species were recorded from the cultures grown on PDA and CLA, respectively. Identification of two chosen isolates was performed by sequencing the EF-1α gene. The TEF sequence of isolate JBL12 showed 100% similarity with several F. tricinctum sequences and sequence of JBL539 showed 99% identity with several F. acuminatum sequences and they were deposited in the NCBI GenBank. Based on the results of the morphological and molecular identification, isolates JBL12 and JBL539 were identified as F. tricinctum and F. acuminatum, respectively, as new causal agents of garlic bulbs rot in Serbia. Specific primers were designed for the PCR identification of the F. tricinctum.", publisher = "Matica Srpska", journal = "Zbornik Matice srpske za prirodne nauke", title = "Morphological and molecular identification of Fusarium tricinctum and Fusarium acuminatum as causal agents of garlic bulbs rot in Serbia", pages = "277-271", volume = "133", doi = "10.2298/ZMSPN1733271I" }
Ignjatov, M., Bjelić, D., Nikolić, Z., Milošević, D., Marinković, J., Ivanović, Ž.,& Gvozdanović-Varga, J.. (2017). Morphological and molecular identification of Fusarium tricinctum and Fusarium acuminatum as causal agents of garlic bulbs rot in Serbia. in Zbornik Matice srpske za prirodne nauke Matica Srpska., 133, 271-277. https://doi.org/10.2298/ZMSPN1733271I
Ignjatov M, Bjelić D, Nikolić Z, Milošević D, Marinković J, Ivanović Ž, Gvozdanović-Varga J. Morphological and molecular identification of Fusarium tricinctum and Fusarium acuminatum as causal agents of garlic bulbs rot in Serbia. in Zbornik Matice srpske za prirodne nauke. 2017;133:271-277. doi:10.2298/ZMSPN1733271I .
Ignjatov, Maja, Bjelić, Dragana, Nikolić, Zorica, Milošević, Dragana, Marinković, Jelena, Ivanović, Žarko, Gvozdanović-Varga, Jelica, "Morphological and molecular identification of Fusarium tricinctum and Fusarium acuminatum as causal agents of garlic bulbs rot in Serbia" in Zbornik Matice srpske za prirodne nauke, 133 (2017):271-277, https://doi.org/10.2298/ZMSPN1733271I . .