BACTERIOPHAGE-BASED BIOCONTROL AGAINST PLANT PATHOGENIC BACTERIA
Authors
Kovacs, TamasBali, Dominika
Solti-Hodovan, Agnes
Obradović, Aleksa
![](/themes/MiragePlantaRum/images/orcid.png)
Gašić, Katarina
![](/themes/MiragePlantaRum/images/orcid.png)
Stefani, Emilio
Altin, Irem
Gopalakrishnan, Chelappan
Fieseler, Lars
Ravasz, Szabolcs
Rakhely, Gabor
Conference object (Published version)
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Show full item recordAbstract
Bacterial plant diseases can cause significant economic losses worldwide. There is an urgent need for the development
and application of sustainable biocontrol agents against plant pathogenic bacteria. Bacteriophages are promising antimicrobial
agents, as it was demonstrated for five phytopathogenic bacteria. Phages infecting Acidovorax citrulli, causing
seedling blight and bacterial fruit blotch of cucurbits, were isolated and characterized. Lytic life cycle and some of the biological
characteristics of investigated phage strains indicated their potential in the control of watermelon fruit blotch. Xanthomonas
euvesicatoria causes bacterial spot disease of pepper and tomato. Phage KΦ1 was isolated from the rhizosphere
of pepper plants showing symptoms of bacterial spot. Foliar applications of the unformulated KΦ1 phage suspension
effectively controlled pepper bacterial spot compared to the standard treatment and the untreated control. Xanthomonas
oryzae pv. oryzae (Xoo) is the ...most devastating agent of rice, causing its bacterial leaf blight (BLB). We isolated and characterized
ten OP2-like bacteriophages. Laboratory efficacy trials strengthened the hypothesis that these bacteriophages
can control BLB effectively. A bacteriophage cocktail protected rice plants against Xoo successfully during a field trial.
Xanthomonas arboricola pv. juglandis (Xaj) causes walnut blight disease. Bacteriophages against Xaj were isolated from
Italian and Hungarian samples and characterized. Two phages were tested individually for their ability to suppress symptoms
in walnut plantlets following bacterial inoculation. A significant reduction in the degree of symptoms was observed
following phage application when compared to the control. We formulated a cocktail containing six bacteriophages and
implemented field trials by spraying the phage suspension on different Juglans regia varieties. The applied bacteriophage
cocktail protected walnut trees against Xaj effectively, albeit the efficacy was different depending on which variety was
treated. Erwinia amylovora causes fire blight disease of Rosaceae plants. 125 bacteriophages were isolated from soil, and
aerial samples were taken in Hungary, Romania, and Spain, from which 55 showed lytic plaque morphology. Based on
host range analysis conducted on 101 bacterium strains, 27 phages were subject of detailed characterization. The applied
bacteriophage cocktail carefully formulated protected apple trees against E. amylovora during two field trials. A special
permit was issued for marketing the Erwiphage bacteriophage-based biopesticide in 2012. This product was the first bacteriophage-
based biocontrol agent against E. amylovora and the second one among all bacteriophages-based biopesticide
that was available on the market worldwide. Our results support that bacteriophages are potent biocontrol agents against
phytopathogenic bacteria.
Source:
Annual Meeting of the Hungarian Society for Microbiology and the XIVth Fermentation Colloquium, October 14–16, 2020 Kecskemét, Hungary, 2021Publisher:
- University of Pecs
Funding / projects:
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200010 (Institute for Plant Protection and Environment, Belgrade) (RS-MESTD-inst-2020-200010)
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Institution/Community
IZBISTY - CONF AU - Kovacs, Tamas AU - Bali, Dominika AU - Solti-Hodovan, Agnes AU - Obradović, Aleksa AU - Gašić, Katarina AU - Stefani, Emilio AU - Altin, Irem AU - Gopalakrishnan, Chelappan AU - Fieseler, Lars AU - Ravasz, Szabolcs AU - Rakhely, Gabor PY - 2021 UR - https://plantarum.izbis.bg.ac.rs/handle/123456789/1271 AB - Bacterial plant diseases can cause significant economic losses worldwide. There is an urgent need for the development and application of sustainable biocontrol agents against plant pathogenic bacteria. Bacteriophages are promising antimicrobial agents, as it was demonstrated for five phytopathogenic bacteria. Phages infecting Acidovorax citrulli, causing seedling blight and bacterial fruit blotch of cucurbits, were isolated and characterized. Lytic life cycle and some of the biological characteristics of investigated phage strains indicated their potential in the control of watermelon fruit blotch. Xanthomonas euvesicatoria causes bacterial spot disease of pepper and tomato. Phage KΦ1 was isolated from the rhizosphere of pepper plants showing symptoms of bacterial spot. Foliar applications of the unformulated KΦ1 phage suspension effectively controlled pepper bacterial spot compared to the standard treatment and the untreated control. Xanthomonas oryzae pv. oryzae (Xoo) is the most devastating agent of rice, causing its bacterial leaf blight (BLB). We isolated and characterized ten OP2-like bacteriophages. Laboratory efficacy trials strengthened the hypothesis that these bacteriophages can control BLB effectively. A bacteriophage cocktail protected rice plants against Xoo successfully during a field trial. Xanthomonas arboricola pv. juglandis (Xaj) causes walnut blight disease. Bacteriophages against Xaj were isolated from Italian and Hungarian samples and characterized. Two phages were tested individually for their ability to suppress symptoms in walnut plantlets following bacterial inoculation. A significant reduction in the degree of symptoms was observed following phage application when compared to the control. We formulated a cocktail containing six bacteriophages and implemented field trials by spraying the phage suspension on different Juglans regia varieties. The applied bacteriophage cocktail protected walnut trees against Xaj effectively, albeit the efficacy was different depending on which variety was treated. Erwinia amylovora causes fire blight disease of Rosaceae plants. 125 bacteriophages were isolated from soil, and aerial samples were taken in Hungary, Romania, and Spain, from which 55 showed lytic plaque morphology. Based on host range analysis conducted on 101 bacterium strains, 27 phages were subject of detailed characterization. The applied bacteriophage cocktail carefully formulated protected apple trees against E. amylovora during two field trials. A special permit was issued for marketing the Erwiphage bacteriophage-based biopesticide in 2012. This product was the first bacteriophage- based biocontrol agent against E. amylovora and the second one among all bacteriophages-based biopesticide that was available on the market worldwide. Our results support that bacteriophages are potent biocontrol agents against phytopathogenic bacteria. PB - University of Pecs C3 - Annual Meeting of the Hungarian Society for Microbiology and the XIVth Fermentation Colloquium, October 14–16, 2020 Kecskemét, Hungary T1 - BACTERIOPHAGE-BASED BIOCONTROL AGAINST PLANT PATHOGENIC BACTERIA ER -
@conference{ author = "Kovacs, Tamas and Bali, Dominika and Solti-Hodovan, Agnes and Obradović, Aleksa and Gašić, Katarina and Stefani, Emilio and Altin, Irem and Gopalakrishnan, Chelappan and Fieseler, Lars and Ravasz, Szabolcs and Rakhely, Gabor", year = "2021", abstract = "Bacterial plant diseases can cause significant economic losses worldwide. There is an urgent need for the development and application of sustainable biocontrol agents against plant pathogenic bacteria. Bacteriophages are promising antimicrobial agents, as it was demonstrated for five phytopathogenic bacteria. Phages infecting Acidovorax citrulli, causing seedling blight and bacterial fruit blotch of cucurbits, were isolated and characterized. Lytic life cycle and some of the biological characteristics of investigated phage strains indicated their potential in the control of watermelon fruit blotch. Xanthomonas euvesicatoria causes bacterial spot disease of pepper and tomato. Phage KΦ1 was isolated from the rhizosphere of pepper plants showing symptoms of bacterial spot. Foliar applications of the unformulated KΦ1 phage suspension effectively controlled pepper bacterial spot compared to the standard treatment and the untreated control. Xanthomonas oryzae pv. oryzae (Xoo) is the most devastating agent of rice, causing its bacterial leaf blight (BLB). We isolated and characterized ten OP2-like bacteriophages. Laboratory efficacy trials strengthened the hypothesis that these bacteriophages can control BLB effectively. A bacteriophage cocktail protected rice plants against Xoo successfully during a field trial. Xanthomonas arboricola pv. juglandis (Xaj) causes walnut blight disease. Bacteriophages against Xaj were isolated from Italian and Hungarian samples and characterized. Two phages were tested individually for their ability to suppress symptoms in walnut plantlets following bacterial inoculation. A significant reduction in the degree of symptoms was observed following phage application when compared to the control. We formulated a cocktail containing six bacteriophages and implemented field trials by spraying the phage suspension on different Juglans regia varieties. The applied bacteriophage cocktail protected walnut trees against Xaj effectively, albeit the efficacy was different depending on which variety was treated. Erwinia amylovora causes fire blight disease of Rosaceae plants. 125 bacteriophages were isolated from soil, and aerial samples were taken in Hungary, Romania, and Spain, from which 55 showed lytic plaque morphology. Based on host range analysis conducted on 101 bacterium strains, 27 phages were subject of detailed characterization. The applied bacteriophage cocktail carefully formulated protected apple trees against E. amylovora during two field trials. A special permit was issued for marketing the Erwiphage bacteriophage-based biopesticide in 2012. This product was the first bacteriophage- based biocontrol agent against E. amylovora and the second one among all bacteriophages-based biopesticide that was available on the market worldwide. Our results support that bacteriophages are potent biocontrol agents against phytopathogenic bacteria.", publisher = "University of Pecs", journal = "Annual Meeting of the Hungarian Society for Microbiology and the XIVth Fermentation Colloquium, October 14–16, 2020 Kecskemét, Hungary", title = "BACTERIOPHAGE-BASED BIOCONTROL AGAINST PLANT PATHOGENIC BACTERIA" }
Kovacs, T., Bali, D., Solti-Hodovan, A., Obradović, A., Gašić, K., Stefani, E., Altin, I., Gopalakrishnan, C., Fieseler, L., Ravasz, S.,& Rakhely, G.. (2021). BACTERIOPHAGE-BASED BIOCONTROL AGAINST PLANT PATHOGENIC BACTERIA. in Annual Meeting of the Hungarian Society for Microbiology and the XIVth Fermentation Colloquium, October 14–16, 2020 Kecskemét, Hungary University of Pecs..
Kovacs T, Bali D, Solti-Hodovan A, Obradović A, Gašić K, Stefani E, Altin I, Gopalakrishnan C, Fieseler L, Ravasz S, Rakhely G. BACTERIOPHAGE-BASED BIOCONTROL AGAINST PLANT PATHOGENIC BACTERIA. in Annual Meeting of the Hungarian Society for Microbiology and the XIVth Fermentation Colloquium, October 14–16, 2020 Kecskemét, Hungary. 2021;..
Kovacs, Tamas, Bali, Dominika, Solti-Hodovan, Agnes, Obradović, Aleksa, Gašić, Katarina, Stefani, Emilio, Altin, Irem, Gopalakrishnan, Chelappan, Fieseler, Lars, Ravasz, Szabolcs, Rakhely, Gabor, "BACTERIOPHAGE-BASED BIOCONTROL AGAINST PLANT PATHOGENIC BACTERIA" in Annual Meeting of the Hungarian Society for Microbiology and the XIVth Fermentation Colloquium, October 14–16, 2020 Kecskemét, Hungary (2021).