Antagonistic potential of Pseudomonas graminis strains against some economically important xanthomonads
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The plant phyllosphere is colonized by various microorganisms including bacteria that could act as
natural antagonists of plant pathogens. In order to find an alternative to chemical pesticides in
plant protection, our study was focused on epiphytic bacteria as potential biocontrol agents.
Among 104 bacterial strains collected from apple phyllosphere in Serbia, four strains showed
potential to inhibit growth of Xanthomonas euvesicatoria strains in preliminary in vitro screening.
According to physiological and biochemical characteristics, as well as 16S rRNA and rpoD genes
sequences, all four strains were identified as Pseudomonas graminis. Its antagonistic activity was
further tested against some of the economically most important xanthomonads in vitro. Plates of
KB medium were spot inoculated with antagonistic strains, and after 48 h of incubation, cultures
were scarped from the medium afterwards plates were exposed to chloroform vapor. Plates were
subsequently flooded with ...suspension of each pathogenic strain in 0.7 % water agar (conc. 107
CFU/ml). Antagonistic activity was evaluated by measuring the widths of the inhibition zones after
48 h of incubation at 27 °C. Results of our research revealed that all tested strains showed
antagonistic activity against X. euvesicatoria, X. vesicatoria, X. gardneri, X. perforans, X. arboricola
pv. juglandis and X. hortorum pv. pelargoni by producing an inhibition zone of 5-27 mm. Our study
showed that strains of P. graminis are able to inhibit Xanthomonas spp. growth in vitro, suggesting
their potential use as a biocontrol agent in plant disease control.
Izvor:
4th Annual Conference of the EuroXanth COST Action Virtual Conference 28–30 June 2021 Ralf Koebnik, Katarina Gašić, Aleksa Obradović (eds.), 2021, 90-Izdavač:
- University of Belgrade – Faculty of Agriculture
Finansiranje / projekti:
- Ministarstvo nauke, tehnološkog razvoja i inovacija Republike Srbije, institucionalno finansiranje - 200010 (Institut za zaštitu bilja i životnu sredinu, Beograd) (RS-MESTD-inst-2020-200010)
Institucija/grupa
IZBISTY - CONF AU - Gašić, Katarina AU - Zlatković, Nevena AU - Paunović, Marija PY - 2021 UR - https://plantarum.izbis.bg.ac.rs/handle/123456789/1273 AB - The plant phyllosphere is colonized by various microorganisms including bacteria that could act as natural antagonists of plant pathogens. In order to find an alternative to chemical pesticides in plant protection, our study was focused on epiphytic bacteria as potential biocontrol agents. Among 104 bacterial strains collected from apple phyllosphere in Serbia, four strains showed potential to inhibit growth of Xanthomonas euvesicatoria strains in preliminary in vitro screening. According to physiological and biochemical characteristics, as well as 16S rRNA and rpoD genes sequences, all four strains were identified as Pseudomonas graminis. Its antagonistic activity was further tested against some of the economically most important xanthomonads in vitro. Plates of KB medium were spot inoculated with antagonistic strains, and after 48 h of incubation, cultures were scarped from the medium afterwards plates were exposed to chloroform vapor. Plates were subsequently flooded with suspension of each pathogenic strain in 0.7 % water agar (conc. 107 CFU/ml). Antagonistic activity was evaluated by measuring the widths of the inhibition zones after 48 h of incubation at 27 °C. Results of our research revealed that all tested strains showed antagonistic activity against X. euvesicatoria, X. vesicatoria, X. gardneri, X. perforans, X. arboricola pv. juglandis and X. hortorum pv. pelargoni by producing an inhibition zone of 5-27 mm. Our study showed that strains of P. graminis are able to inhibit Xanthomonas spp. growth in vitro, suggesting their potential use as a biocontrol agent in plant disease control. PB - University of Belgrade – Faculty of Agriculture C3 - 4th Annual Conference of the EuroXanth COST Action Virtual Conference 28–30 June 2021 Ralf Koebnik, Katarina Gašić, Aleksa Obradović (eds.) T1 - Antagonistic potential of Pseudomonas graminis strains against some economically important xanthomonads SP - 90 ER -
@conference{ author = "Gašić, Katarina and Zlatković, Nevena and Paunović, Marija", year = "2021", abstract = "The plant phyllosphere is colonized by various microorganisms including bacteria that could act as natural antagonists of plant pathogens. In order to find an alternative to chemical pesticides in plant protection, our study was focused on epiphytic bacteria as potential biocontrol agents. Among 104 bacterial strains collected from apple phyllosphere in Serbia, four strains showed potential to inhibit growth of Xanthomonas euvesicatoria strains in preliminary in vitro screening. According to physiological and biochemical characteristics, as well as 16S rRNA and rpoD genes sequences, all four strains were identified as Pseudomonas graminis. Its antagonistic activity was further tested against some of the economically most important xanthomonads in vitro. Plates of KB medium were spot inoculated with antagonistic strains, and after 48 h of incubation, cultures were scarped from the medium afterwards plates were exposed to chloroform vapor. Plates were subsequently flooded with suspension of each pathogenic strain in 0.7 % water agar (conc. 107 CFU/ml). Antagonistic activity was evaluated by measuring the widths of the inhibition zones after 48 h of incubation at 27 °C. Results of our research revealed that all tested strains showed antagonistic activity against X. euvesicatoria, X. vesicatoria, X. gardneri, X. perforans, X. arboricola pv. juglandis and X. hortorum pv. pelargoni by producing an inhibition zone of 5-27 mm. Our study showed that strains of P. graminis are able to inhibit Xanthomonas spp. growth in vitro, suggesting their potential use as a biocontrol agent in plant disease control.", publisher = "University of Belgrade – Faculty of Agriculture", journal = "4th Annual Conference of the EuroXanth COST Action Virtual Conference 28–30 June 2021 Ralf Koebnik, Katarina Gašić, Aleksa Obradović (eds.)", title = "Antagonistic potential of Pseudomonas graminis strains against some economically important xanthomonads", pages = "90" }
Gašić, K., Zlatković, N.,& Paunović, M.. (2021). Antagonistic potential of Pseudomonas graminis strains against some economically important xanthomonads. in 4th Annual Conference of the EuroXanth COST Action Virtual Conference 28–30 June 2021 Ralf Koebnik, Katarina Gašić, Aleksa Obradović (eds.) University of Belgrade – Faculty of Agriculture., 90.
Gašić K, Zlatković N, Paunović M. Antagonistic potential of Pseudomonas graminis strains against some economically important xanthomonads. in 4th Annual Conference of the EuroXanth COST Action Virtual Conference 28–30 June 2021 Ralf Koebnik, Katarina Gašić, Aleksa Obradović (eds.). 2021;:90..
Gašić, Katarina, Zlatković, Nevena, Paunović, Marija, "Antagonistic potential of Pseudomonas graminis strains against some economically important xanthomonads" in 4th Annual Conference of the EuroXanth COST Action Virtual Conference 28–30 June 2021 Ralf Koebnik, Katarina Gašić, Aleksa Obradović (eds.) (2021):90.