Efficacy evaluation of synthetic fungicides and biofungicides against Fusarium wilt in blueberry
Autori
Ristić, Danijela![](/themes/MiragePlantaRum/images/orcid.png)
Vučurović, Ivan
![](/themes/MiragePlantaRum/images/orcid.png)
Blagojević, Jovana
![](/themes/MiragePlantaRum/images/orcid.png)
Keserović, Vukašin
![](/themes/MiragePlantaRum/images/orcid.png)
Starović, Mira
![](/themes/MiragePlantaRum/images/orcid.png)
Trkulja, Nenad
![](/themes/MiragePlantaRum/images/orcid.png)
Aleksić, Goran
![](/themes/MiragePlantaRum/images/orcid.png)
Konferencijski prilog (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
Fusarium wilt poses a significant challenge to blueberry production worldwide and recent reports suggest it is posing a threat to sustainability and expansion of this crop in Serbia. During the survey in 2019 in Sopot, Serbia, symptoms of leaf wilting, reduced plant growth, decreased yield and fruit quality of blueberries were observed. Subsequent morphological and molecular analysis identified Fusarium sporotrichoides as the causal agent of the disease. Addressing this threat, this study aims to evaluate the effectiveness of the widely used synthetic fungicides, trifloxystrobin, metalaxyl+fluazinam, and difenoconazole alongside innovative biological agents comprising of three combinations of lactic acid bacteria (Lactobacillus plantarum, Lactobacillus rhamnosus >103CFU/g), phototrophic bacteria (Rhodopseudomonas palustris >103CFU/g) and yeast sugar molasses (Saccharomyces cerevisiae >103CFU/g), marked as: EM1, EM5 and EM AGRO, using in vitro methods. Minimum inhibitory concentrations ...(MIC) were determined via microdilution tests in 96-well plates, with spore suspensions adjusted to 5x104 in 100 μl per well, incubated at 25°C for 5 days. MIC values were analyzed using STATISTICA v.7, with Duncan’s multiple range tests to differentiate the means at p<0.05. The highest degree of inhibition for F. sporotrichoides was demonstrated by difenoconazole (MIC: 0.13 μg/ml) and a combination of metalaxyl and fluazinam (MIC: 3.63 μg/ml). Trifloxystrobin showed a moderate inhibition (MIC: 15.00 μg/ml). All three combinations of lactic acid, phototrophic bacteria and yeast sugar molasses showed a significant antifungal effect against F. sporotrichoides (MIC<50.00 μg/ml). The microbial mixture EM5 demonstrated the highest potency (MIC: 38.80 μg/ml). This study not only validates the effectiveness of these antifungal strategies against Fusarium wilt in blueberries, but also encourages a balanced approach to combating this disease. Combining chemical and biological treatments contributes to the preservation of ecosystems and human health by reducing the effect of chemicals and enhancing food safety.
Ključne reči:
blueberry / Fusarium sporotrichoides / synthetic fungicides / biological agentsIzvor:
XIII International Symposium on Agricultural Sciences "AgroReS 2024" 27-30 May 2024, Trebinje, Bosnia and Herzegovina, 2024, 61-Izdavač:
- University of Banja Luka, Faculty of Agriculture
Finansiranje / projekti:
- Ministarstvo nauke, tehnološkog razvoja i inovacija Republike Srbije, institucionalno finansiranje - 200010 (Institut za zaštitu bilja i životnu sredinu, Beograd) (RS-MESTD-inst-2020-200010)
Institucija/grupa
IZBISTY - CONF AU - Ristić, Danijela AU - Vučurović, Ivan AU - Blagojević, Jovana AU - Keserović, Vukašin AU - Starović, Mira AU - Trkulja, Nenad AU - Aleksić, Goran PY - 2024 UR - https://plantarum.izbis.bg.ac.rs/handle/123456789/1312 AB - Fusarium wilt poses a significant challenge to blueberry production worldwide and recent reports suggest it is posing a threat to sustainability and expansion of this crop in Serbia. During the survey in 2019 in Sopot, Serbia, symptoms of leaf wilting, reduced plant growth, decreased yield and fruit quality of blueberries were observed. Subsequent morphological and molecular analysis identified Fusarium sporotrichoides as the causal agent of the disease. Addressing this threat, this study aims to evaluate the effectiveness of the widely used synthetic fungicides, trifloxystrobin, metalaxyl+fluazinam, and difenoconazole alongside innovative biological agents comprising of three combinations of lactic acid bacteria (Lactobacillus plantarum, Lactobacillus rhamnosus >103CFU/g), phototrophic bacteria (Rhodopseudomonas palustris >103CFU/g) and yeast sugar molasses (Saccharomyces cerevisiae >103CFU/g), marked as: EM1, EM5 and EM AGRO, using in vitro methods. Minimum inhibitory concentrations (MIC) were determined via microdilution tests in 96-well plates, with spore suspensions adjusted to 5x104 in 100 μl per well, incubated at 25°C for 5 days. MIC values were analyzed using STATISTICA v.7, with Duncan’s multiple range tests to differentiate the means at p<0.05. The highest degree of inhibition for F. sporotrichoides was demonstrated by difenoconazole (MIC: 0.13 μg/ml) and a combination of metalaxyl and fluazinam (MIC: 3.63 μg/ml). Trifloxystrobin showed a moderate inhibition (MIC: 15.00 μg/ml). All three combinations of lactic acid, phototrophic bacteria and yeast sugar molasses showed a significant antifungal effect against F. sporotrichoides (MIC<50.00 μg/ml). The microbial mixture EM5 demonstrated the highest potency (MIC: 38.80 μg/ml). This study not only validates the effectiveness of these antifungal strategies against Fusarium wilt in blueberries, but also encourages a balanced approach to combating this disease. Combining chemical and biological treatments contributes to the preservation of ecosystems and human health by reducing the effect of chemicals and enhancing food safety. PB - University of Banja Luka, Faculty of Agriculture C3 - XIII International Symposium on Agricultural Sciences "AgroReS 2024" 27-30 May 2024, Trebinje, Bosnia and Herzegovina T1 - Efficacy evaluation of synthetic fungicides and biofungicides against Fusarium wilt in blueberry SP - 61 ER -
@conference{ author = "Ristić, Danijela and Vučurović, Ivan and Blagojević, Jovana and Keserović, Vukašin and Starović, Mira and Trkulja, Nenad and Aleksić, Goran", year = "2024", abstract = "Fusarium wilt poses a significant challenge to blueberry production worldwide and recent reports suggest it is posing a threat to sustainability and expansion of this crop in Serbia. During the survey in 2019 in Sopot, Serbia, symptoms of leaf wilting, reduced plant growth, decreased yield and fruit quality of blueberries were observed. Subsequent morphological and molecular analysis identified Fusarium sporotrichoides as the causal agent of the disease. Addressing this threat, this study aims to evaluate the effectiveness of the widely used synthetic fungicides, trifloxystrobin, metalaxyl+fluazinam, and difenoconazole alongside innovative biological agents comprising of three combinations of lactic acid bacteria (Lactobacillus plantarum, Lactobacillus rhamnosus >103CFU/g), phototrophic bacteria (Rhodopseudomonas palustris >103CFU/g) and yeast sugar molasses (Saccharomyces cerevisiae >103CFU/g), marked as: EM1, EM5 and EM AGRO, using in vitro methods. Minimum inhibitory concentrations (MIC) were determined via microdilution tests in 96-well plates, with spore suspensions adjusted to 5x104 in 100 μl per well, incubated at 25°C for 5 days. MIC values were analyzed using STATISTICA v.7, with Duncan’s multiple range tests to differentiate the means at p<0.05. The highest degree of inhibition for F. sporotrichoides was demonstrated by difenoconazole (MIC: 0.13 μg/ml) and a combination of metalaxyl and fluazinam (MIC: 3.63 μg/ml). Trifloxystrobin showed a moderate inhibition (MIC: 15.00 μg/ml). All three combinations of lactic acid, phototrophic bacteria and yeast sugar molasses showed a significant antifungal effect against F. sporotrichoides (MIC<50.00 μg/ml). The microbial mixture EM5 demonstrated the highest potency (MIC: 38.80 μg/ml). This study not only validates the effectiveness of these antifungal strategies against Fusarium wilt in blueberries, but also encourages a balanced approach to combating this disease. Combining chemical and biological treatments contributes to the preservation of ecosystems and human health by reducing the effect of chemicals and enhancing food safety.", publisher = "University of Banja Luka, Faculty of Agriculture", journal = "XIII International Symposium on Agricultural Sciences "AgroReS 2024" 27-30 May 2024, Trebinje, Bosnia and Herzegovina", title = "Efficacy evaluation of synthetic fungicides and biofungicides against Fusarium wilt in blueberry", pages = "61" }
Ristić, D., Vučurović, I., Blagojević, J., Keserović, V., Starović, M., Trkulja, N.,& Aleksić, G.. (2024). Efficacy evaluation of synthetic fungicides and biofungicides against Fusarium wilt in blueberry. in XIII International Symposium on Agricultural Sciences "AgroReS 2024" 27-30 May 2024, Trebinje, Bosnia and Herzegovina University of Banja Luka, Faculty of Agriculture., 61.
Ristić D, Vučurović I, Blagojević J, Keserović V, Starović M, Trkulja N, Aleksić G. Efficacy evaluation of synthetic fungicides and biofungicides against Fusarium wilt in blueberry. in XIII International Symposium on Agricultural Sciences "AgroReS 2024" 27-30 May 2024, Trebinje, Bosnia and Herzegovina. 2024;:61..
Ristić, Danijela, Vučurović, Ivan, Blagojević, Jovana, Keserović, Vukašin, Starović, Mira, Trkulja, Nenad, Aleksić, Goran, "Efficacy evaluation of synthetic fungicides and biofungicides against Fusarium wilt in blueberry" in XIII International Symposium on Agricultural Sciences "AgroReS 2024" 27-30 May 2024, Trebinje, Bosnia and Herzegovina (2024):61.