Molecular characterization of grapevine rupestris stem pitting-associated virus isolates infecting grapevines in Serbia
Authors
Stanković, IvanaRistić, Danijela
![](/themes/MiragePlantaRum/images/orcid.png)
Vučurović, Ivan
![](/themes/MiragePlantaRum/images/orcid.png)
Kovačević, Dušica
Krstić, Branka
Zečević, Katarina
Conference object (Published version)
Metadata
Show full item recordAbstract
Grapevine rupestris stem pitting-associated virus (GRSPaV) occurs worldwide and is often considered to be one of the most prevalent grapevine viruses. It is involved in the graft-transmissible disease rupestris stem pitting of the rugose wood complex, as well as in the vein necrosis and Syrah decline, although its role in all diseases is not yet clear. In addition, GRSPaV is also found in grapevine cultivars in a latent state. After the first detection, two-year survey (2021-2022) was carried out to determine the presence and distribution of the virus in Serbia and to examine its genetic diversity. A total of 55 samples were collected and analyzed by the multiplex reverse transcription-polymerase chain reaction (mRT-PCR) using primers for nine viruses that commonly infect grapevine: arabis mosaic virus (ArMV), grapevine fanleaf virus (GFV), grapevine virus A (GVA) and B (GVB), GRSPaV, grapevine fleck virus (GFkV), grapevine leafroll-associated virus-1 (GLRaV-1), -2 (GLRaV-2), and -3 (G...LRaV-3). The presence of GRSPaV was detected in 66.6% of the samples tested in single or mixed infections with GVA, GLRaV-1, -3, GFkV and/or ArMV. For further GRSPaV molecular characterization, seven mRT-PCR positive isolates were selected and a fragment of the coat protein (CP) gene was partially sequenced. Multiple alignments of the sequences revealed nucleotide identities from 83.9% to 97.9%, indicating variability of the GRSPaV population in Serbia. Phylogenetic analysis revealed that genetic variability of GRSPaV comprising three molecular variants is present in Serbian grapevine genotypes. Although GRSPaV is considered a virus of minor importance, the virus may pose a risk for severe decline symptoms caused by either single or complex infections with other viruses. This study has also shown that the virus is highly prevalent in Serbia and that enhanced phytosanitary measures are needed to exclude GRSPaV in vines used for vineyard propagation and planting.
Keywords:
grapevine / grapevine rupestris stem pitting-associated virus / CP gene / phylogentic analysisSource:
XIII International Symposium on Agricultural Sciences "AgroReS 2024" 27-30 May 2024, Trebinje, Bosnia and Herzegovina, 2024, 72-Publisher:
- University of Banja Luka, Faculty of Agriculture
Funding / projects:
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200010 (Institute for Plant Protection and Environment, Belgrade) (RS-MESTD-inst-2020-200010)
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200116 (University of Belgrade, Faculty of Agriculture) (RS-MESTD-inst-2020-200116)
Collections
Institution/Community
IZBISTY - CONF AU - Stanković, Ivana AU - Ristić, Danijela AU - Vučurović, Ivan AU - Kovačević, Dušica AU - Krstić, Branka AU - Zečević, Katarina PY - 2024 UR - https://plantarum.izbis.bg.ac.rs/handle/123456789/1313 AB - Grapevine rupestris stem pitting-associated virus (GRSPaV) occurs worldwide and is often considered to be one of the most prevalent grapevine viruses. It is involved in the graft-transmissible disease rupestris stem pitting of the rugose wood complex, as well as in the vein necrosis and Syrah decline, although its role in all diseases is not yet clear. In addition, GRSPaV is also found in grapevine cultivars in a latent state. After the first detection, two-year survey (2021-2022) was carried out to determine the presence and distribution of the virus in Serbia and to examine its genetic diversity. A total of 55 samples were collected and analyzed by the multiplex reverse transcription-polymerase chain reaction (mRT-PCR) using primers for nine viruses that commonly infect grapevine: arabis mosaic virus (ArMV), grapevine fanleaf virus (GFV), grapevine virus A (GVA) and B (GVB), GRSPaV, grapevine fleck virus (GFkV), grapevine leafroll-associated virus-1 (GLRaV-1), -2 (GLRaV-2), and -3 (GLRaV-3). The presence of GRSPaV was detected in 66.6% of the samples tested in single or mixed infections with GVA, GLRaV-1, -3, GFkV and/or ArMV. For further GRSPaV molecular characterization, seven mRT-PCR positive isolates were selected and a fragment of the coat protein (CP) gene was partially sequenced. Multiple alignments of the sequences revealed nucleotide identities from 83.9% to 97.9%, indicating variability of the GRSPaV population in Serbia. Phylogenetic analysis revealed that genetic variability of GRSPaV comprising three molecular variants is present in Serbian grapevine genotypes. Although GRSPaV is considered a virus of minor importance, the virus may pose a risk for severe decline symptoms caused by either single or complex infections with other viruses. This study has also shown that the virus is highly prevalent in Serbia and that enhanced phytosanitary measures are needed to exclude GRSPaV in vines used for vineyard propagation and planting. PB - University of Banja Luka, Faculty of Agriculture C3 - XIII International Symposium on Agricultural Sciences "AgroReS 2024" 27-30 May 2024, Trebinje, Bosnia and Herzegovina T1 - Molecular characterization of grapevine rupestris stem pitting-associated virus isolates infecting grapevines in Serbia SP - 72 ER -
@conference{ author = "Stanković, Ivana and Ristić, Danijela and Vučurović, Ivan and Kovačević, Dušica and Krstić, Branka and Zečević, Katarina", year = "2024", abstract = "Grapevine rupestris stem pitting-associated virus (GRSPaV) occurs worldwide and is often considered to be one of the most prevalent grapevine viruses. It is involved in the graft-transmissible disease rupestris stem pitting of the rugose wood complex, as well as in the vein necrosis and Syrah decline, although its role in all diseases is not yet clear. In addition, GRSPaV is also found in grapevine cultivars in a latent state. After the first detection, two-year survey (2021-2022) was carried out to determine the presence and distribution of the virus in Serbia and to examine its genetic diversity. A total of 55 samples were collected and analyzed by the multiplex reverse transcription-polymerase chain reaction (mRT-PCR) using primers for nine viruses that commonly infect grapevine: arabis mosaic virus (ArMV), grapevine fanleaf virus (GFV), grapevine virus A (GVA) and B (GVB), GRSPaV, grapevine fleck virus (GFkV), grapevine leafroll-associated virus-1 (GLRaV-1), -2 (GLRaV-2), and -3 (GLRaV-3). The presence of GRSPaV was detected in 66.6% of the samples tested in single or mixed infections with GVA, GLRaV-1, -3, GFkV and/or ArMV. For further GRSPaV molecular characterization, seven mRT-PCR positive isolates were selected and a fragment of the coat protein (CP) gene was partially sequenced. Multiple alignments of the sequences revealed nucleotide identities from 83.9% to 97.9%, indicating variability of the GRSPaV population in Serbia. Phylogenetic analysis revealed that genetic variability of GRSPaV comprising three molecular variants is present in Serbian grapevine genotypes. Although GRSPaV is considered a virus of minor importance, the virus may pose a risk for severe decline symptoms caused by either single or complex infections with other viruses. This study has also shown that the virus is highly prevalent in Serbia and that enhanced phytosanitary measures are needed to exclude GRSPaV in vines used for vineyard propagation and planting.", publisher = "University of Banja Luka, Faculty of Agriculture", journal = "XIII International Symposium on Agricultural Sciences "AgroReS 2024" 27-30 May 2024, Trebinje, Bosnia and Herzegovina", title = "Molecular characterization of grapevine rupestris stem pitting-associated virus isolates infecting grapevines in Serbia", pages = "72" }
Stanković, I., Ristić, D., Vučurović, I., Kovačević, D., Krstić, B.,& Zečević, K.. (2024). Molecular characterization of grapevine rupestris stem pitting-associated virus isolates infecting grapevines in Serbia. in XIII International Symposium on Agricultural Sciences "AgroReS 2024" 27-30 May 2024, Trebinje, Bosnia and Herzegovina University of Banja Luka, Faculty of Agriculture., 72.
Stanković I, Ristić D, Vučurović I, Kovačević D, Krstić B, Zečević K. Molecular characterization of grapevine rupestris stem pitting-associated virus isolates infecting grapevines in Serbia. in XIII International Symposium on Agricultural Sciences "AgroReS 2024" 27-30 May 2024, Trebinje, Bosnia and Herzegovina. 2024;:72..
Stanković, Ivana, Ristić, Danijela, Vučurović, Ivan, Kovačević, Dušica, Krstić, Branka, Zečević, Katarina, "Molecular characterization of grapevine rupestris stem pitting-associated virus isolates infecting grapevines in Serbia" in XIII International Symposium on Agricultural Sciences "AgroReS 2024" 27-30 May 2024, Trebinje, Bosnia and Herzegovina (2024):72.