GENOMIC AND PHENOTYPIC CHARACTERIZATION OF Pseudomonas syringae pv. syringae STRAINS ISOLATED FROM RASPBERRY AND BLACKBERRY IN SERBIA
2019
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During May of 2016. severe blight symptoms were observed on raspberry and blackberry in several locations of western Serbia. Symptoms appeared as brown to black lesions on leaves, petioles, internodes, shoots and developing laterals causing necrosis and death of the cane. Occasionally, glistening bacterial ooze was observed on the infected tissue surface.
From symptomatic leaves, petioles and internodes uniform bacterial colonies were repeatedly isolated. Twenty two representative strains were selected for further studies based on the locality, cultivar and host plant. In addition, eight strains from two previous outbreaks were included in the study. Strains were characterized based on basic biochemical properties, LOPAT tests and pathogenicity on detached raspberry canes. To determine genetic variability among isolated strains, repP.CR analysis with BOXAIR, ERIC1 R/ERIC2 and REP1 R/REP21 primers was used.
Isolated bacterial strains developed whitish colonies on NA medium. They ...were Gram and oxidase negative, fluorescent on King's medium B, levan positive, arginin dehydrolase negative, produced a hypersensitive response on tobacco leaves, and did not rot potato slices (LOPAT group la).The strains hydrolised gelatine and aesculin, but did not produce thyrosinase. In the pathogenicity test all strains caused typical symptoms of blackening of leafs and petioles, followed by wilting, necrosis and death of the inoculated canes, resemblit ,g the natural infection. Symptoms were not observed on the control plants.
Our results show that genomic profiles obtained in rep-PCR were identical for 29 strains except for strain KBI 222. Additionally, both of the generated profiles were different from the profiles of the Pseudomonas syringae pv. syringae (Pss) pathotype strain and the reference Pss strain used in this study. Same results were obtained with the ERIC and BOX primers: all strains had identical genomic profiles except strain KBI 222. This strain had unique genomic patterns different from all the other strains isolated from raspberry or blackberry and different from the pathotype or the reference Pss strains. This strain was isolated from raspberry cv. Willamette near Kosjeric and did not differ in any other studied property.
Although variation in genomic pattern of one strain was discovered in the rep-PCR, identical patterns for all other strains indicate low genetic diversity in the population. Results obtained in this study showed that the bacterial population causing Pseudomonas blight on raspberry and blackberry is highly homogenous and closely related, both in biochemical and genetic properties,
Izvor:
VIII Congres on Plant Protection, November 25-29.2019., Zlatibor, Serbia, 2019, 182-Izdavač:
- Društvo za zaštitu bilja
Finansiranje / projekti:
- Razvoj integrisanih sistema upravljanja štetnim organizmima u biljnoj proizvodnji sa ciljem prevazilaženja rezistentnosti i unapređenja kvaliteta i bezbednosti hrane (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-46008)
Institucija/grupa
IZBISTY - CONF AU - Ivanović, Milan AU - Prokić, Anđelka AU - Gašić, Katarina AU - Menković, Jelena AU - Obradović, Aleksa PY - 2019 UR - https://plantarum.izbis.bg.ac.rs/handle/123456789/1334 AB - During May of 2016. severe blight symptoms were observed on raspberry and blackberry in several locations of western Serbia. Symptoms appeared as brown to black lesions on leaves, petioles, internodes, shoots and developing laterals causing necrosis and death of the cane. Occasionally, glistening bacterial ooze was observed on the infected tissue surface. From symptomatic leaves, petioles and internodes uniform bacterial colonies were repeatedly isolated. Twenty two representative strains were selected for further studies based on the locality, cultivar and host plant. In addition, eight strains from two previous outbreaks were included in the study. Strains were characterized based on basic biochemical properties, LOPAT tests and pathogenicity on detached raspberry canes. To determine genetic variability among isolated strains, repP.CR analysis with BOXAIR, ERIC1 R/ERIC2 and REP1 R/REP21 primers was used. Isolated bacterial strains developed whitish colonies on NA medium. They were Gram and oxidase negative, fluorescent on King's medium B, levan positive, arginin dehydrolase negative, produced a hypersensitive response on tobacco leaves, and did not rot potato slices (LOPAT group la).The strains hydrolised gelatine and aesculin, but did not produce thyrosinase. In the pathogenicity test all strains caused typical symptoms of blackening of leafs and petioles, followed by wilting, necrosis and death of the inoculated canes, resemblit ,g the natural infection. Symptoms were not observed on the control plants. Our results show that genomic profiles obtained in rep-PCR were identical for 29 strains except for strain KBI 222. Additionally, both of the generated profiles were different from the profiles of the Pseudomonas syringae pv. syringae (Pss) pathotype strain and the reference Pss strain used in this study. Same results were obtained with the ERIC and BOX primers: all strains had identical genomic profiles except strain KBI 222. This strain had unique genomic patterns different from all the other strains isolated from raspberry or blackberry and different from the pathotype or the reference Pss strains. This strain was isolated from raspberry cv. Willamette near Kosjeric and did not differ in any other studied property. Although variation in genomic pattern of one strain was discovered in the rep-PCR, identical patterns for all other strains indicate low genetic diversity in the population. Results obtained in this study showed that the bacterial population causing Pseudomonas blight on raspberry and blackberry is highly homogenous and closely related, both in biochemical and genetic properties, PB - Društvo za zaštitu bilja C3 - VIII Congres on Plant Protection, November 25-29.2019., Zlatibor, Serbia T1 - GENOMIC AND PHENOTYPIC CHARACTERIZATION OF Pseudomonas syringae pv. syringae STRAINS ISOLATED FROM RASPBERRY AND BLACKBERRY IN SERBIA SP - 182 ER -
@conference{ author = "Ivanović, Milan and Prokić, Anđelka and Gašić, Katarina and Menković, Jelena and Obradović, Aleksa", year = "2019", abstract = "During May of 2016. severe blight symptoms were observed on raspberry and blackberry in several locations of western Serbia. Symptoms appeared as brown to black lesions on leaves, petioles, internodes, shoots and developing laterals causing necrosis and death of the cane. Occasionally, glistening bacterial ooze was observed on the infected tissue surface. From symptomatic leaves, petioles and internodes uniform bacterial colonies were repeatedly isolated. Twenty two representative strains were selected for further studies based on the locality, cultivar and host plant. In addition, eight strains from two previous outbreaks were included in the study. Strains were characterized based on basic biochemical properties, LOPAT tests and pathogenicity on detached raspberry canes. To determine genetic variability among isolated strains, repP.CR analysis with BOXAIR, ERIC1 R/ERIC2 and REP1 R/REP21 primers was used. Isolated bacterial strains developed whitish colonies on NA medium. They were Gram and oxidase negative, fluorescent on King's medium B, levan positive, arginin dehydrolase negative, produced a hypersensitive response on tobacco leaves, and did not rot potato slices (LOPAT group la).The strains hydrolised gelatine and aesculin, but did not produce thyrosinase. In the pathogenicity test all strains caused typical symptoms of blackening of leafs and petioles, followed by wilting, necrosis and death of the inoculated canes, resemblit ,g the natural infection. Symptoms were not observed on the control plants. Our results show that genomic profiles obtained in rep-PCR were identical for 29 strains except for strain KBI 222. Additionally, both of the generated profiles were different from the profiles of the Pseudomonas syringae pv. syringae (Pss) pathotype strain and the reference Pss strain used in this study. Same results were obtained with the ERIC and BOX primers: all strains had identical genomic profiles except strain KBI 222. This strain had unique genomic patterns different from all the other strains isolated from raspberry or blackberry and different from the pathotype or the reference Pss strains. This strain was isolated from raspberry cv. Willamette near Kosjeric and did not differ in any other studied property. Although variation in genomic pattern of one strain was discovered in the rep-PCR, identical patterns for all other strains indicate low genetic diversity in the population. Results obtained in this study showed that the bacterial population causing Pseudomonas blight on raspberry and blackberry is highly homogenous and closely related, both in biochemical and genetic properties,", publisher = "Društvo za zaštitu bilja", journal = "VIII Congres on Plant Protection, November 25-29.2019., Zlatibor, Serbia", title = "GENOMIC AND PHENOTYPIC CHARACTERIZATION OF Pseudomonas syringae pv. syringae STRAINS ISOLATED FROM RASPBERRY AND BLACKBERRY IN SERBIA", pages = "182" }
Ivanović, M., Prokić, A., Gašić, K., Menković, J.,& Obradović, A.. (2019). GENOMIC AND PHENOTYPIC CHARACTERIZATION OF Pseudomonas syringae pv. syringae STRAINS ISOLATED FROM RASPBERRY AND BLACKBERRY IN SERBIA. in VIII Congres on Plant Protection, November 25-29.2019., Zlatibor, Serbia Društvo za zaštitu bilja., 182.
Ivanović M, Prokić A, Gašić K, Menković J, Obradović A. GENOMIC AND PHENOTYPIC CHARACTERIZATION OF Pseudomonas syringae pv. syringae STRAINS ISOLATED FROM RASPBERRY AND BLACKBERRY IN SERBIA. in VIII Congres on Plant Protection, November 25-29.2019., Zlatibor, Serbia. 2019;:182..
Ivanović, Milan, Prokić, Anđelka, Gašić, Katarina, Menković, Jelena, Obradović, Aleksa, "GENOMIC AND PHENOTYPIC CHARACTERIZATION OF Pseudomonas syringae pv. syringae STRAINS ISOLATED FROM RASPBERRY AND BLACKBERRY IN SERBIA" in VIII Congres on Plant Protection, November 25-29.2019., Zlatibor, Serbia (2019):182.