Stolbur phytoplasma infection of kale crops (Brassica oleracea var. gemmifera L.) in Serbia
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2011
Authors
Trkulja, NenadIvanović, Žarko
Pfaf-Dolovac, Erika
Dolovac, Nenad
Živković, Svetlana
Jović, Jelena
Mitrović, Milana
Article (Published version)
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Kale plants (Brassica oleracea var. gemmifera L.) showing symptoms of reddening on leaf and petioles and overall stunting were found on locality Pancevo (south Banat) during inspection on vegetable crops carried out in 2010 in Serbia. The phytoplasma associated with the disease was detected through nested PCR with primer pairs P1/P7 and R16F2n/R2 followed by RFLP analysis with restriction enzyme Msel. A total of 10 symptomatic and 4 asymptomatic plants were collected and analyzed for the presence of phytoplasma. Nested PCR amplified 16S ribosomal RNA fragments of phytoplasma in all samples of kale with symptoms, while all asymptomatic plants tested negative. Digestion with Msel identified in infected kale the same pattern as a reference strain of stolbur phytoplasma belonging to 16SrXII-A group. Amplification of the elongation factor tuf gene and digestion with HpaII restriction enzyme was performed for genotype differentiation of stolbur phytoplasmas detected in kale. Digestion of tuf... gene indicated presence of tuf-type b of stolbur phytoplasma in all symptomatic kale plants. This is the first report of stolbur phytoplasma infecting kale crops in Serbia.
Keywords:
stolbur phytoplasma / Serbia / tuf gene / Brassica oleraceae var. gemmiferaSource:
Bulletin of Insectology, 2011, 64Publisher:
- Alma Mater Studiorum, Univ Bologna, Bologna
Funding / projects:
- Development of integrated approach in plant protection for control harmful organisms (RS-MESTD-Technological Development (TD or TR)-31018)
- Agrobiodiversity and land-use change in Serbia: an integrated biodiversity assessment of key functional groups of arthropods and plant pathogens (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-43001)
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IZBISTY - JOUR AU - Trkulja, Nenad AU - Ivanović, Žarko AU - Pfaf-Dolovac, Erika AU - Dolovac, Nenad AU - Živković, Svetlana AU - Jović, Jelena AU - Mitrović, Milana PY - 2011 UR - https://plantarum.izbis.bg.ac.rs/handle/123456789/157 AB - Kale plants (Brassica oleracea var. gemmifera L.) showing symptoms of reddening on leaf and petioles and overall stunting were found on locality Pancevo (south Banat) during inspection on vegetable crops carried out in 2010 in Serbia. The phytoplasma associated with the disease was detected through nested PCR with primer pairs P1/P7 and R16F2n/R2 followed by RFLP analysis with restriction enzyme Msel. A total of 10 symptomatic and 4 asymptomatic plants were collected and analyzed for the presence of phytoplasma. Nested PCR amplified 16S ribosomal RNA fragments of phytoplasma in all samples of kale with symptoms, while all asymptomatic plants tested negative. Digestion with Msel identified in infected kale the same pattern as a reference strain of stolbur phytoplasma belonging to 16SrXII-A group. Amplification of the elongation factor tuf gene and digestion with HpaII restriction enzyme was performed for genotype differentiation of stolbur phytoplasmas detected in kale. Digestion of tuf gene indicated presence of tuf-type b of stolbur phytoplasma in all symptomatic kale plants. This is the first report of stolbur phytoplasma infecting kale crops in Serbia. PB - Alma Mater Studiorum, Univ Bologna, Bologna T2 - Bulletin of Insectology T1 - Stolbur phytoplasma infection of kale crops (Brassica oleracea var. gemmifera L.) in Serbia VL - 64 ER -
@article{ author = "Trkulja, Nenad and Ivanović, Žarko and Pfaf-Dolovac, Erika and Dolovac, Nenad and Živković, Svetlana and Jović, Jelena and Mitrović, Milana", year = "2011", abstract = "Kale plants (Brassica oleracea var. gemmifera L.) showing symptoms of reddening on leaf and petioles and overall stunting were found on locality Pancevo (south Banat) during inspection on vegetable crops carried out in 2010 in Serbia. The phytoplasma associated with the disease was detected through nested PCR with primer pairs P1/P7 and R16F2n/R2 followed by RFLP analysis with restriction enzyme Msel. A total of 10 symptomatic and 4 asymptomatic plants were collected and analyzed for the presence of phytoplasma. Nested PCR amplified 16S ribosomal RNA fragments of phytoplasma in all samples of kale with symptoms, while all asymptomatic plants tested negative. Digestion with Msel identified in infected kale the same pattern as a reference strain of stolbur phytoplasma belonging to 16SrXII-A group. Amplification of the elongation factor tuf gene and digestion with HpaII restriction enzyme was performed for genotype differentiation of stolbur phytoplasmas detected in kale. Digestion of tuf gene indicated presence of tuf-type b of stolbur phytoplasma in all symptomatic kale plants. This is the first report of stolbur phytoplasma infecting kale crops in Serbia.", publisher = "Alma Mater Studiorum, Univ Bologna, Bologna", journal = "Bulletin of Insectology", title = "Stolbur phytoplasma infection of kale crops (Brassica oleracea var. gemmifera L.) in Serbia", volume = "64" }
Trkulja, N., Ivanović, Ž., Pfaf-Dolovac, E., Dolovac, N., Živković, S., Jović, J.,& Mitrović, M.. (2011). Stolbur phytoplasma infection of kale crops (Brassica oleracea var. gemmifera L.) in Serbia. in Bulletin of Insectology Alma Mater Studiorum, Univ Bologna, Bologna., 64.
Trkulja N, Ivanović Ž, Pfaf-Dolovac E, Dolovac N, Živković S, Jović J, Mitrović M. Stolbur phytoplasma infection of kale crops (Brassica oleracea var. gemmifera L.) in Serbia. in Bulletin of Insectology. 2011;64..
Trkulja, Nenad, Ivanović, Žarko, Pfaf-Dolovac, Erika, Dolovac, Nenad, Živković, Svetlana, Jović, Jelena, Mitrović, Milana, "Stolbur phytoplasma infection of kale crops (Brassica oleracea var. gemmifera L.) in Serbia" in Bulletin of Insectology, 64 (2011).