TY  - JOUR
AU  - Iličić, Renata
AU  - Jelušić, Aleksandra
AU  - Barać, Goran
AU  - Nikolić, Dušan
AU  - Stošić, Nemanja
AU  - Scortichini, Marco
AU  - Popović Milovanović, Tatjana
PY  - 2024
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/1245
AB  - In August 2020, the unusual appearance of crown gall symptoms was observed on the
tobacco plants (hybrid PVH2310) grown in fields in the Golubinci (Srem district, Serbia) locality. The
causal agent isolated from galls located on tobacco roots formed circular, convex, and glistening
light blue colonies, and then dark to olive-green-colored bacterial colonies on a semi-selective D1
medium. Molecular analysis based on multiplex PCR and multi-locus sequence analysis (MLSA)
using concatenated sequences of the atpD, dnaK, glnA, and rpoB genes as well as 16S rRNA identified
Serbian tobacco isolates such as Agrobacterium tumefaciens (biovar 1). Two duplex PCR methods
confirmed the presence of the virD2 and virC genes in tobacco isolates. Pathogenicity tests performed
on carrot discs and squash fruits resulted in tumor/gall formation after 12 to 16 days post inoculation,
respectively. Pathogenicity was also confirmed on tobacco plants, where isolates caused tumor
development 21−25 days after inoculation. API 50 CH generated results regarding the biochemical
features of the Serbian tobacco isolates. As A. tumefaciens (biovar 1) as a cause of tobacco crown gall
has previously been documented solely in Japan, there is presently no data on its wider occurrence.
Therefore, this first detailed investigation of A. tumefaciens isolated from naturally infected tobacco in
Serbia will contribute to a better understanding of it at the global level.
PB  - MDPI Basel
T2  - Agronomy
T1  - In-Depth Characterization of Crown Gall Disease of Tobacco in Serbia
IS  - 4
SP  - 851
VL  - 14
DO  - 10.3390/agronomy14040851
ER  - 

@article{
author = "Iličić, Renata and Jelušić, Aleksandra and Barać, Goran and Nikolić, Dušan and Stošić, Nemanja and Scortichini, Marco and Popović Milovanović, Tatjana",
year = "2024",
abstract = "In August 2020, the unusual appearance of crown gall symptoms was observed on the
tobacco plants (hybrid PVH2310) grown in fields in the Golubinci (Srem district, Serbia) locality. The
causal agent isolated from galls located on tobacco roots formed circular, convex, and glistening
light blue colonies, and then dark to olive-green-colored bacterial colonies on a semi-selective D1
medium. Molecular analysis based on multiplex PCR and multi-locus sequence analysis (MLSA)
using concatenated sequences of the atpD, dnaK, glnA, and rpoB genes as well as 16S rRNA identified
Serbian tobacco isolates such as Agrobacterium tumefaciens (biovar 1). Two duplex PCR methods
confirmed the presence of the virD2 and virC genes in tobacco isolates. Pathogenicity tests performed
on carrot discs and squash fruits resulted in tumor/gall formation after 12 to 16 days post inoculation,
respectively. Pathogenicity was also confirmed on tobacco plants, where isolates caused tumor
development 21−25 days after inoculation. API 50 CH generated results regarding the biochemical
features of the Serbian tobacco isolates. As A. tumefaciens (biovar 1) as a cause of tobacco crown gall
has previously been documented solely in Japan, there is presently no data on its wider occurrence.
Therefore, this first detailed investigation of A. tumefaciens isolated from naturally infected tobacco in
Serbia will contribute to a better understanding of it at the global level.",
publisher = "MDPI Basel",
journal = "Agronomy",
title = "In-Depth Characterization of Crown Gall Disease of Tobacco in Serbia",
number = "4",
pages = "851",
volume = "14",
doi = "10.3390/agronomy14040851"
}

Iličić, R., Jelušić, A., Barać, G., Nikolić, D., Stošić, N., Scortichini, M.,& Popović Milovanović, T.. (2024). In-Depth Characterization of Crown Gall Disease of Tobacco in Serbia. in Agronomy
MDPI Basel., 14(4), 851.
https://doi.org/10.3390/agronomy14040851

Iličić R, Jelušić A, Barać G, Nikolić D, Stošić N, Scortichini M, Popović Milovanović T. In-Depth Characterization of Crown Gall Disease of Tobacco in Serbia. in Agronomy. 2024;14(4):851.
doi:10.3390/agronomy14040851 .

Iličić, Renata, Jelušić, Aleksandra, Barać, Goran, Nikolić, Dušan, Stošić, Nemanja, Scortichini, Marco, Popović Milovanović, Tatjana, "In-Depth Characterization of Crown Gall Disease of Tobacco in Serbia" in Agronomy, 14, no. 4 (2024):851,
https://doi.org/10.3390/agronomy14040851 . .

TY  - CONF
AU  - Popović Milovanović, Tatjana
AU  - Iličić, Renata
AU  - Trkulja, Nenad
AU  - Trkulja, Vojislav
AU  - Zečević, Katarina
AU  - Jelušić, Aleksandra
PY  - 2023
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/1196
AB  - Bakteriozna mrljavost plodova lubenice (Acidovorax citrulli) predstavlja širom sveta
ozbiljnu pretnju uključujući uzgajivače i proizvođače semena i rasada gajenih vrsta biljaka iz
porodice Cucurbitacae. Krajem 1980-ih, bakterija A. citrulli se nakon epidemijskih pojava na
lubenici u SAD proširila na druga područja i useve (dinja, tikva, bundeva, krastavac). Do danas
su opisane dve genetički različite populacije A. citrulli. U Srbiji je prvi nalaz A. citrulli
zabeležen 2014. godine na lubenici u lokalitetima Bačke i Srema. Međutim, uprkos
sprovedenim eradikativnim merama i naporima u upravljanju ovim patogenom, ponovo je
detektovan 2018., 2021., i 2022. godine na području Mačve i Srema. U cilju boljeg
razumevanja genetičke strukture populacija A. citrulli prisutnih u Srbiji, u ovom radu je
korišćena analiza sekvenci više genskih lokusa (MLSA) primenom konzerviranih gena i gena
virulentnosti.
Tokom rada korišćena su četiri reprezentativna soja A. citrulli, pod šiframa Ac414
(Čelarevo, 2014), Ac818 (Šabac, 2018), Ac221 (Ašanja, 2021) i Ac322 (Hrtkovci, 2022),
odabrana na osnovu prethodnih rezultata dobijenih umnožavanjem ponavljajućih sekvenci
DNK (rep-PCR). Za MLSA je korišćeno devet gena, i to osam konzervativnih (gltA, gmc, gyrB,
lepA, phaC, pilT, trpB, ugpB) i jedan gen virulentnosti (Aave_1548). PCR program se sastojao
od inicijalne denaturacije (5 min na 95 °C), praćene sa 30 ciklusa denaturacije (30 s na 95 °C),
11
hibridizacije (30 s na 60 °C za konzervativne gene i 55 °C za gen virulentnosti Aave_1548) i
elongacije (30 s za konzervativne gene i 90 s za gen Aave_1548 na 72 °C); i finalne elongacije
(5 min na 72 °C). Amplifikovani produkti su sekvencirani u Eurofins Genomics (Hamburg,
Nemačka). Kvalitet dobijenih sekvenci je manuelno pregledan, nakon čega je za svaki od četiri
testirana soja napravljena multigenska sekvenca na osnovu svih sekvenciranih gena.
mMultigenske sekvence testiranih i referentnih sojeva A. citrulli preuzetih iz baze podataka
Nacionalnog centra za biotehnološke informacije (NCBI) korišćene su za konstruisanje
„Neighbor-joining” filogenetskog stabla. Na osnovu rezultata filogenetske analize, dokazano je
da sojevi A. citrulli izolovani iz prirodno zaraženih plodova lubenice u Srbiji (2014-2022)
pripadaju dvema reprezentativnim genetičkim grupama opisanim u svetu (grupe I i II). Grupi
I su pripadali sojevi izolovani 2021. i 2022. godine, a grupi II sojevi izolovani 2014. i 2018.
godine. Rezultati dobijeni u ovom radu ukazuju na puteve introdukcije i širenja A. citrulli u
Srbiji.
PB  - Društvo za zaštitu bilja
C3  - XVII simpozijum o zaštiti bilja, Zlatibor 2023, 27-30. novembar, Zbornik rezimea radova
T1  - Utvrđivanje genetičke strukture populacija Acidovorax citrulli u Srbiji
EP  - 11
SP  - 10
ER  - 

@conference{
author = "Popović Milovanović, Tatjana and Iličić, Renata and Trkulja, Nenad and Trkulja, Vojislav and Zečević, Katarina and Jelušić, Aleksandra",
year = "2023",
abstract = "Bakteriozna mrljavost plodova lubenice (Acidovorax citrulli) predstavlja širom sveta
ozbiljnu pretnju uključujući uzgajivače i proizvođače semena i rasada gajenih vrsta biljaka iz
porodice Cucurbitacae. Krajem 1980-ih, bakterija A. citrulli se nakon epidemijskih pojava na
lubenici u SAD proširila na druga područja i useve (dinja, tikva, bundeva, krastavac). Do danas
su opisane dve genetički različite populacije A. citrulli. U Srbiji je prvi nalaz A. citrulli
zabeležen 2014. godine na lubenici u lokalitetima Bačke i Srema. Međutim, uprkos
sprovedenim eradikativnim merama i naporima u upravljanju ovim patogenom, ponovo je
detektovan 2018., 2021., i 2022. godine na području Mačve i Srema. U cilju boljeg
razumevanja genetičke strukture populacija A. citrulli prisutnih u Srbiji, u ovom radu je
korišćena analiza sekvenci više genskih lokusa (MLSA) primenom konzerviranih gena i gena
virulentnosti.
Tokom rada korišćena su četiri reprezentativna soja A. citrulli, pod šiframa Ac414
(Čelarevo, 2014), Ac818 (Šabac, 2018), Ac221 (Ašanja, 2021) i Ac322 (Hrtkovci, 2022),
odabrana na osnovu prethodnih rezultata dobijenih umnožavanjem ponavljajućih sekvenci
DNK (rep-PCR). Za MLSA je korišćeno devet gena, i to osam konzervativnih (gltA, gmc, gyrB,
lepA, phaC, pilT, trpB, ugpB) i jedan gen virulentnosti (Aave_1548). PCR program se sastojao
od inicijalne denaturacije (5 min na 95 °C), praćene sa 30 ciklusa denaturacije (30 s na 95 °C),
11
hibridizacije (30 s na 60 °C za konzervativne gene i 55 °C za gen virulentnosti Aave_1548) i
elongacije (30 s za konzervativne gene i 90 s za gen Aave_1548 na 72 °C); i finalne elongacije
(5 min na 72 °C). Amplifikovani produkti su sekvencirani u Eurofins Genomics (Hamburg,
Nemačka). Kvalitet dobijenih sekvenci je manuelno pregledan, nakon čega je za svaki od četiri
testirana soja napravljena multigenska sekvenca na osnovu svih sekvenciranih gena.
mMultigenske sekvence testiranih i referentnih sojeva A. citrulli preuzetih iz baze podataka
Nacionalnog centra za biotehnološke informacije (NCBI) korišćene su za konstruisanje
„Neighbor-joining” filogenetskog stabla. Na osnovu rezultata filogenetske analize, dokazano je
da sojevi A. citrulli izolovani iz prirodno zaraženih plodova lubenice u Srbiji (2014-2022)
pripadaju dvema reprezentativnim genetičkim grupama opisanim u svetu (grupe I i II). Grupi
I su pripadali sojevi izolovani 2021. i 2022. godine, a grupi II sojevi izolovani 2014. i 2018.
godine. Rezultati dobijeni u ovom radu ukazuju na puteve introdukcije i širenja A. citrulli u
Srbiji.",
publisher = "Društvo za zaštitu bilja",
journal = "XVII simpozijum o zaštiti bilja, Zlatibor 2023, 27-30. novembar, Zbornik rezimea radova",
title = "Utvrđivanje genetičke strukture populacija Acidovorax citrulli u Srbiji",
pages = "11-10"
}

Popović Milovanović, T., Iličić, R., Trkulja, N., Trkulja, V., Zečević, K.,& Jelušić, A.. (2023). Utvrđivanje genetičke strukture populacija Acidovorax citrulli u Srbiji. in XVII simpozijum o zaštiti bilja, Zlatibor 2023, 27-30. novembar, Zbornik rezimea radova
Društvo za zaštitu bilja., 10-11.

Popović Milovanović T, Iličić R, Trkulja N, Trkulja V, Zečević K, Jelušić A. Utvrđivanje genetičke strukture populacija Acidovorax citrulli u Srbiji. in XVII simpozijum o zaštiti bilja, Zlatibor 2023, 27-30. novembar, Zbornik rezimea radova. 2023;:10-11..

Popović Milovanović, Tatjana, Iličić, Renata, Trkulja, Nenad, Trkulja, Vojislav, Zečević, Katarina, Jelušić, Aleksandra, "Utvrđivanje genetičke strukture populacija Acidovorax citrulli u Srbiji" in XVII simpozijum o zaštiti bilja, Zlatibor 2023, 27-30. novembar, Zbornik rezimea radova (2023):10-11.

TY  - JOUR
AU  - Jelušić, Aleksandra
AU  - Mitrović, Petar
AU  - Iličić, Renata
AU  - Milovanović, Predrag
AU  - Stanković, Slaviša
AU  - Popović Milovanović, Tatjana
PY  - 2023
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/1137
AB  - The aim of this work was to identify and characterize the pectolytic bacteria responsible for the emergence of bacterial soft rot on two summer cabbage hybrids (Cheers F1 and Hippo F1) grown in the Futog locality (Bačka, Vojvodina), known for the five-century-long tradition of cabbage cultivation in Serbia. Symptoms manifesting as soft lesions on outer head leaves were observed during August 2021, while the inner tissues were macerated, featuring cream to black discoloration. As the affected tissue decomposed, it exuded a specific odor. Disease incidence ranged from 15% to 25%. A total of 67 isolates producing pits on crystal violet pectate (CVP) medium were characterized for their phenotypic and genotypic features. The pathogenicity was confirmed on cabbage heads. Findings yielded by the repetitive element palindromic-polymerase chain reaction (rep-PCR) technique confirmed interspecies diversity between cabbage isolates, as well as intraspecies genetic diversity within the P. carotovorum group of isolates. Based on multilocus sequence typing (MLST) using genes dnaX, mdh, icdA, and proA, five representative isolates were identified as Pectobacterium carotovorum (Cheers F1 and Hippo F1), while two were identified as Pectobacterium versatile (Hippo F1) and Pectobacterium odoriferum (Hippo F1), respectively, indicating the presence of diverse Pectobacterium species even in combined infection in the same field. Among the obtained isolates, P. carotovorum was the most prevalent species (62.69%), while P. versatile and P. odoriferum were less represented (contributing by 19.40% and 17.91%, respectively). Multilocus sequence analysis (MLSA) performed with concatenated sequences of four housekeeping genes (proA, dnaX, icdA, and mdh) and constructed a neighbor-joining phylogenetic tree enabled insight into the phylogenetic position of the Serbian cabbage Pectobacterium isolates. Bacterium P. odoriferum was found to be the most virulent species for cabbage, followed by P. versatile, while all three species had comparable virulence with respect to potato. The results obtained in this work provide a better understanding of the spreading routes and abundance of different Pectobacterium spp. in Serbia.
PB  - MDPI
T2  - Microorganisms
T1  - Diversity of Bacterial Soft Rot-Causing Pectobacterium Species Affecting Cabbage in Serbia
IS  - 2
SP  - 335
VL  - 11
DO  - 10.3390/microorganisms11020335
ER  - 

@article{
author = "Jelušić, Aleksandra and Mitrović, Petar and Iličić, Renata and Milovanović, Predrag and Stanković, Slaviša and Popović Milovanović, Tatjana",
year = "2023",
abstract = "The aim of this work was to identify and characterize the pectolytic bacteria responsible for the emergence of bacterial soft rot on two summer cabbage hybrids (Cheers F1 and Hippo F1) grown in the Futog locality (Bačka, Vojvodina), known for the five-century-long tradition of cabbage cultivation in Serbia. Symptoms manifesting as soft lesions on outer head leaves were observed during August 2021, while the inner tissues were macerated, featuring cream to black discoloration. As the affected tissue decomposed, it exuded a specific odor. Disease incidence ranged from 15% to 25%. A total of 67 isolates producing pits on crystal violet pectate (CVP) medium were characterized for their phenotypic and genotypic features. The pathogenicity was confirmed on cabbage heads. Findings yielded by the repetitive element palindromic-polymerase chain reaction (rep-PCR) technique confirmed interspecies diversity between cabbage isolates, as well as intraspecies genetic diversity within the P. carotovorum group of isolates. Based on multilocus sequence typing (MLST) using genes dnaX, mdh, icdA, and proA, five representative isolates were identified as Pectobacterium carotovorum (Cheers F1 and Hippo F1), while two were identified as Pectobacterium versatile (Hippo F1) and Pectobacterium odoriferum (Hippo F1), respectively, indicating the presence of diverse Pectobacterium species even in combined infection in the same field. Among the obtained isolates, P. carotovorum was the most prevalent species (62.69%), while P. versatile and P. odoriferum were less represented (contributing by 19.40% and 17.91%, respectively). Multilocus sequence analysis (MLSA) performed with concatenated sequences of four housekeeping genes (proA, dnaX, icdA, and mdh) and constructed a neighbor-joining phylogenetic tree enabled insight into the phylogenetic position of the Serbian cabbage Pectobacterium isolates. Bacterium P. odoriferum was found to be the most virulent species for cabbage, followed by P. versatile, while all three species had comparable virulence with respect to potato. The results obtained in this work provide a better understanding of the spreading routes and abundance of different Pectobacterium spp. in Serbia.",
publisher = "MDPI",
journal = "Microorganisms",
title = "Diversity of Bacterial Soft Rot-Causing Pectobacterium Species Affecting Cabbage in Serbia",
number = "2",
pages = "335",
volume = "11",
doi = "10.3390/microorganisms11020335"
}

Jelušić, A., Mitrović, P., Iličić, R., Milovanović, P., Stanković, S.,& Popović Milovanović, T.. (2023). Diversity of Bacterial Soft Rot-Causing Pectobacterium Species Affecting Cabbage in Serbia. in Microorganisms
MDPI., 11(2), 335.
https://doi.org/10.3390/microorganisms11020335

Jelušić A, Mitrović P, Iličić R, Milovanović P, Stanković S, Popović Milovanović T. Diversity of Bacterial Soft Rot-Causing Pectobacterium Species Affecting Cabbage in Serbia. in Microorganisms. 2023;11(2):335.
doi:10.3390/microorganisms11020335 .

Jelušić, Aleksandra, Mitrović, Petar, Iličić, Renata, Milovanović, Predrag, Stanković, Slaviša, Popović Milovanović, Tatjana, "Diversity of Bacterial Soft Rot-Causing Pectobacterium Species Affecting Cabbage in Serbia" in Microorganisms, 11, no. 2 (2023):335,
https://doi.org/10.3390/microorganisms11020335 . .

TY  - JOUR
AU  - Iličić, Renata
AU  - Jelušić, Aleksandra
AU  - Blagojević, Milan
AU  - Bagi, Ferenc
AU  - Vrandečić, Karolina
AU  - Stanković, Slaviša
AU  - Popović Milovanović, Tatjana
PY  - 2023
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/875
AB  - Pseudomonas strains originating from symptomatic (bacterial spot) leaf tissues of sweet cherry (Topola, Šumadija) and plum (Krušedol Selo, Srem) were isolated during 2016 and 2020, respectively. Based on the findings yielded by classical microbiological methods, LOPAT (+---+), GATTa (--++) and pathogenicity tests performed on detached fruitlets (sweet and sour cherry) and pods (bean pods), all strains were confirmed to belong to P. syringae pv. morsprunorum. The detection of cfl gene allowed strains that belong to race 1 to be identified. The DNA fingerprinting patterns obtained with four rep-PCR (BOX and ERIC), RAPD-PCR (M13), and IS50-PCR (IS50) methods revealed that the seven tested sweet cherry and plum P. s. pv. morsprunorum strains, as well as comparative KBNS71 and the reference strain CFBP 2119, were genetically heterogeneous. Conversely, MLSA based on the four-gene-based scheme (gapA, gltA, gyrB, and rpoD) indicated genetic homogeneity among all tested Serbian sweet cherry and plum strains, as well as P. s. pv. morsprunorum race 1 strains from the NCBI. Although the MLSA findings indicate that the sweet cherry and plum strains used in this study are 100% identical, as they might have different virulence genes, genome sequencing should be performed to eventually find the strain sub-clades based on the host.
PB  - Serbian Genetics Society
T2  - Genetika
T1  - Characterization of Pseudomonas syringae pv. morsprunorum originating from sweet cherry and plum in Serbia
EP  - 176
IS  - 1
SP  - 159
VL  - 55
DO  - 10.2298/gensr2301159i
ER  - 

@article{
author = "Iličić, Renata and Jelušić, Aleksandra and Blagojević, Milan and Bagi, Ferenc and Vrandečić, Karolina and Stanković, Slaviša and Popović Milovanović, Tatjana",
year = "2023",
abstract = "Pseudomonas strains originating from symptomatic (bacterial spot) leaf tissues of sweet cherry (Topola, Šumadija) and plum (Krušedol Selo, Srem) were isolated during 2016 and 2020, respectively. Based on the findings yielded by classical microbiological methods, LOPAT (+---+), GATTa (--++) and pathogenicity tests performed on detached fruitlets (sweet and sour cherry) and pods (bean pods), all strains were confirmed to belong to P. syringae pv. morsprunorum. The detection of cfl gene allowed strains that belong to race 1 to be identified. The DNA fingerprinting patterns obtained with four rep-PCR (BOX and ERIC), RAPD-PCR (M13), and IS50-PCR (IS50) methods revealed that the seven tested sweet cherry and plum P. s. pv. morsprunorum strains, as well as comparative KBNS71 and the reference strain CFBP 2119, were genetically heterogeneous. Conversely, MLSA based on the four-gene-based scheme (gapA, gltA, gyrB, and rpoD) indicated genetic homogeneity among all tested Serbian sweet cherry and plum strains, as well as P. s. pv. morsprunorum race 1 strains from the NCBI. Although the MLSA findings indicate that the sweet cherry and plum strains used in this study are 100% identical, as they might have different virulence genes, genome sequencing should be performed to eventually find the strain sub-clades based on the host.",
publisher = "Serbian Genetics Society",
journal = "Genetika",
title = "Characterization of Pseudomonas syringae pv. morsprunorum originating from sweet cherry and plum in Serbia",
pages = "176-159",
number = "1",
volume = "55",
doi = "10.2298/gensr2301159i"
}

Iličić, R., Jelušić, A., Blagojević, M., Bagi, F., Vrandečić, K., Stanković, S.,& Popović Milovanović, T.. (2023). Characterization of Pseudomonas syringae pv. morsprunorum originating from sweet cherry and plum in Serbia. in Genetika
Serbian Genetics Society., 55(1), 159-176.
https://doi.org/10.2298/gensr2301159i

Iličić R, Jelušić A, Blagojević M, Bagi F, Vrandečić K, Stanković S, Popović Milovanović T. Characterization of Pseudomonas syringae pv. morsprunorum originating from sweet cherry and plum in Serbia. in Genetika. 2023;55(1):159-176.
doi:10.2298/gensr2301159i .

Iličić, Renata, Jelušić, Aleksandra, Blagojević, Milan, Bagi, Ferenc, Vrandečić, Karolina, Stanković, Slaviša, Popović Milovanović, Tatjana, "Characterization of Pseudomonas syringae pv. morsprunorum originating from sweet cherry and plum in Serbia" in Genetika, 55, no. 1 (2023):159-176,
https://doi.org/10.2298/gensr2301159i . .

TY  - JOUR
AU  - Marković, Sanja
AU  - Popović Milovanović, Tatjana
AU  - Jelušić, Aleksandra
AU  - Medić, O.
AU  - Berić, Tanja
AU  - Stanković, Slaviša
PY  - 2023
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/849
AB  - Potato is ranked as one of the most important food crops. Bacterial wilt caused by Ralstonia solanacearum and blackleg and soft rot caused by different species from genera Pectobacterium and Dickeya are considered two of the most important diseases of the potato. Biological control is the optimal strategy for controlling pathogens in crops generally, including bacteria. The present study aimed to evaluate the antagonistic effects of two Bacillus amyloliquefaciens strains, SS-12.6 and SS-38.4, against bacterial pathogens isolated from the potato in Serbia, such as R. solanacearum, Pectobacterium carotovorum, Pectobacterium brasiliense, and Dickeya dianthicola. The diameter of the inhibition zones formed by ethyl-acetate extracts of SS-12.6 and SS-38.4 strains show much higher values than the inhibition zones of supernatants, which implies the main power of these antagonists' potential lies in lipopeptides. The effectiveness of the treatment (19.7–44.5%), based on the difference in weight of potato tubers on the fifth and 15th day after treatment (DAT), showed that the antagonistic strains were almost equally effective in the suppression of P. carotovorum, P. brasiliense, and D. dianthicola strains. Strains SS-12.6 and SS-38.4 exhibited the efficacy in the suppression of R. solanacearum wilt from 28.64 to 60.22%. The analysis of the area under the disease progress (AUDPC) confirmed differences among pathogen control treatments and biocontrol treatments with B. amyloliquefaciens strains in all trials. This study shows that the two B. amyloliquefaciens strains, SS-12.6 and SS-38.4, can potentially be used as biocontrol agents against potato pathogens.
PB  - Elsevier
T2  - Biological Control
T1  - Biological control of major pathogenic bacteria of potato by Bacillus amyloliquefaciens strains SS-12.6 and SS-38.4
SP  - 105238
VL  - 182
DO  - 10.1016/j.biocontrol.2023.105238
ER  - 

@article{
author = "Marković, Sanja and Popović Milovanović, Tatjana and Jelušić, Aleksandra and Medić, O. and Berić, Tanja and Stanković, Slaviša",
year = "2023",
abstract = "Potato is ranked as one of the most important food crops. Bacterial wilt caused by Ralstonia solanacearum and blackleg and soft rot caused by different species from genera Pectobacterium and Dickeya are considered two of the most important diseases of the potato. Biological control is the optimal strategy for controlling pathogens in crops generally, including bacteria. The present study aimed to evaluate the antagonistic effects of two Bacillus amyloliquefaciens strains, SS-12.6 and SS-38.4, against bacterial pathogens isolated from the potato in Serbia, such as R. solanacearum, Pectobacterium carotovorum, Pectobacterium brasiliense, and Dickeya dianthicola. The diameter of the inhibition zones formed by ethyl-acetate extracts of SS-12.6 and SS-38.4 strains show much higher values than the inhibition zones of supernatants, which implies the main power of these antagonists' potential lies in lipopeptides. The effectiveness of the treatment (19.7–44.5%), based on the difference in weight of potato tubers on the fifth and 15th day after treatment (DAT), showed that the antagonistic strains were almost equally effective in the suppression of P. carotovorum, P. brasiliense, and D. dianthicola strains. Strains SS-12.6 and SS-38.4 exhibited the efficacy in the suppression of R. solanacearum wilt from 28.64 to 60.22%. The analysis of the area under the disease progress (AUDPC) confirmed differences among pathogen control treatments and biocontrol treatments with B. amyloliquefaciens strains in all trials. This study shows that the two B. amyloliquefaciens strains, SS-12.6 and SS-38.4, can potentially be used as biocontrol agents against potato pathogens.",
publisher = "Elsevier",
journal = "Biological Control",
title = "Biological control of major pathogenic bacteria of potato by Bacillus amyloliquefaciens strains SS-12.6 and SS-38.4",
pages = "105238",
volume = "182",
doi = "10.1016/j.biocontrol.2023.105238"
}

Marković, S., Popović Milovanović, T., Jelušić, A., Medić, O., Berić, T.,& Stanković, S.. (2023). Biological control of major pathogenic bacteria of potato by Bacillus amyloliquefaciens strains SS-12.6 and SS-38.4. in Biological Control
Elsevier., 182, 105238.
https://doi.org/10.1016/j.biocontrol.2023.105238

Marković S, Popović Milovanović T, Jelušić A, Medić O, Berić T, Stanković S. Biological control of major pathogenic bacteria of potato by Bacillus amyloliquefaciens strains SS-12.6 and SS-38.4. in Biological Control. 2023;182:105238.
doi:10.1016/j.biocontrol.2023.105238 .

Marković, Sanja, Popović Milovanović, Tatjana, Jelušić, Aleksandra, Medić, O., Berić, Tanja, Stanković, Slaviša, "Biological control of major pathogenic bacteria of potato by Bacillus amyloliquefaciens strains SS-12.6 and SS-38.4" in Biological Control, 182 (2023):105238,
https://doi.org/10.1016/j.biocontrol.2023.105238 . .

TY  - JOUR
AU  - Iličić, Renata
AU  - Popović Milovanović, Tatjana
AU  - Marković, Sanja
AU  - Jelušić, Aleksandra
AU  - Bagi, Ferenc
AU  - Vlajić, Slobodan
AU  - Stanković, Slaviša
PY  - 2021
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/674
AB  - Bacterial canker and leaf spot caused by plant pathogenic bacterium Pseudomonas is among the most destructive cherry diseases worldwide. Nowadays in Serbia, sweet cherry production significantly increased and the new plantations, mainly grown from imported planting material are being raised every year. During spring, 2018 and 2019, occurrence of bacterial canker and leaf spot symptoms was observed on a newly planted sweet cherry plantations in two localities, Zitorada (Southern region) and Karavukovo (Northern region-Vojvodina). Typical P. syringae colonies were isolated on Nutrient Sucrose Agar supplemented with 5% sucrose (NSA). A total of fifteen isolates were selected and identified. Results of the LOPAT test (+---+) determined them to belong to fluorescent Pseudomonas Group Ia, while results of G(+)A(+)T(-)Ta(-) tests indicate presence of Pseudomonas syringae pv. syringae. Pathogenicity was confirmed on immature sweet and sour cherry fruitlets by forming of black, sunken lesions for all tested isolates. Genes syrB and syrD were successfully detected in all tested isolates. DNA sequencing using gapA, gltA, gyrB and rpoD housekeeping genes determined tested isolates to belong to P. s. pv. syringae using the National Center for Biotechnology Information (NCBI) nucleotide BLAST. The Serbian isolates shared 99.47% to 100% (Zitorada) and 99.38% to 100% (Karavukovo) identity with bacterium P. s. pv. syringae. Phylogenetic analysis grouped isolates from Zitorada in one tree cluster, separate from the Karavukovo isolates,indicating presence of two genetically diverse groups of causal pathogen P. s. pv. syringae, obtained from two geographically distinct localities in Serbia. Phylogeographic analysis grouped isolates from Zitorada in multilocus haplotype coded as REz and isolates originated from Karavukovo in multilocus haplotype coded as REk. Considering that during last few years P. syringae continuously occurs mainly in young sweet cherry plantations, where imported material is used for raising, health status check is recommended to be included as obligatory measure when nursery material is used from import.
PB  - Društvo genetičara Srbije
T2  - Genetika
T1  - Genetic diversity of Pseudomonas syringae pv. syringae isolated from sweet cherry in southern and northern regions in Serbia
EP  - 262
IS  - 1
SP  - 247
VL  - 53
DO  - 10.2298/GENSR2101247I
ER  - 

@article{
author = "Iličić, Renata and Popović Milovanović, Tatjana and Marković, Sanja and Jelušić, Aleksandra and Bagi, Ferenc and Vlajić, Slobodan and Stanković, Slaviša",
year = "2021",
abstract = "Bacterial canker and leaf spot caused by plant pathogenic bacterium Pseudomonas is among the most destructive cherry diseases worldwide. Nowadays in Serbia, sweet cherry production significantly increased and the new plantations, mainly grown from imported planting material are being raised every year. During spring, 2018 and 2019, occurrence of bacterial canker and leaf spot symptoms was observed on a newly planted sweet cherry plantations in two localities, Zitorada (Southern region) and Karavukovo (Northern region-Vojvodina). Typical P. syringae colonies were isolated on Nutrient Sucrose Agar supplemented with 5% sucrose (NSA). A total of fifteen isolates were selected and identified. Results of the LOPAT test (+---+) determined them to belong to fluorescent Pseudomonas Group Ia, while results of G(+)A(+)T(-)Ta(-) tests indicate presence of Pseudomonas syringae pv. syringae. Pathogenicity was confirmed on immature sweet and sour cherry fruitlets by forming of black, sunken lesions for all tested isolates. Genes syrB and syrD were successfully detected in all tested isolates. DNA sequencing using gapA, gltA, gyrB and rpoD housekeeping genes determined tested isolates to belong to P. s. pv. syringae using the National Center for Biotechnology Information (NCBI) nucleotide BLAST. The Serbian isolates shared 99.47% to 100% (Zitorada) and 99.38% to 100% (Karavukovo) identity with bacterium P. s. pv. syringae. Phylogenetic analysis grouped isolates from Zitorada in one tree cluster, separate from the Karavukovo isolates,indicating presence of two genetically diverse groups of causal pathogen P. s. pv. syringae, obtained from two geographically distinct localities in Serbia. Phylogeographic analysis grouped isolates from Zitorada in multilocus haplotype coded as REz and isolates originated from Karavukovo in multilocus haplotype coded as REk. Considering that during last few years P. syringae continuously occurs mainly in young sweet cherry plantations, where imported material is used for raising, health status check is recommended to be included as obligatory measure when nursery material is used from import.",
publisher = "Društvo genetičara Srbije",
journal = "Genetika",
title = "Genetic diversity of Pseudomonas syringae pv. syringae isolated from sweet cherry in southern and northern regions in Serbia",
pages = "262-247",
number = "1",
volume = "53",
doi = "10.2298/GENSR2101247I"
}

Iličić, R., Popović Milovanović, T., Marković, S., Jelušić, A., Bagi, F., Vlajić, S.,& Stanković, S.. (2021). Genetic diversity of Pseudomonas syringae pv. syringae isolated from sweet cherry in southern and northern regions in Serbia. in Genetika
Društvo genetičara Srbije., 53(1), 247-262.
https://doi.org/10.2298/GENSR2101247I

Iličić R, Popović Milovanović T, Marković S, Jelušić A, Bagi F, Vlajić S, Stanković S. Genetic diversity of Pseudomonas syringae pv. syringae isolated from sweet cherry in southern and northern regions in Serbia. in Genetika. 2021;53(1):247-262.
doi:10.2298/GENSR2101247I .

Iličić, Renata, Popović Milovanović, Tatjana, Marković, Sanja, Jelušić, Aleksandra, Bagi, Ferenc, Vlajić, Slobodan, Stanković, Slaviša, "Genetic diversity of Pseudomonas syringae pv. syringae isolated from sweet cherry in southern and northern regions in Serbia" in Genetika, 53, no. 1 (2021):247-262,
https://doi.org/10.2298/GENSR2101247I . .

TY  - JOUR
AU  - Popović Milovanović, Tatjana
AU  - Mitrović, Petar
AU  - Jelušić, Aleksandra
AU  - Dimkić, Ivica
AU  - Marjanovic Jeromela, Ana
AU  - Nikolić, Ivan
AU  - Stanković, Slaviša
PY  - 2019
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/942
AB  - The present study provides insight into the diversity of 147 Xanthomonas campestris pv. campestris (Xcc) isolates obtained from six Brassica oleracea vegetable crops (broccoli, cabbage, cauliflower, collard greens, kale, kohlrabi) and the winter oilseed rape crop Brassica napus, collected from different regions in Serbia in 2014. The XCF/XCR pathovar-specific primer set was used for fast preliminary identification. In repetitive sequence-based PCR (BOX, ERIC and REP) of all isolates, a higher level of genetic diversity was found in winter oilseed rape isolates compared to isolates from the other hosts. ERIC and REP-PCR showed the highest heterogeneity, with 10 and nine banding patterns, respectively. The REP-PCR results showed the highest correlation (70%) with those obtained with multilocus sequence analysis (MLSA), performed with 10 housekeeping genes (fusA, gap-1, gltA, gyrB1, lacF, lepA, rpoD, dnaK, fyuA and gyrB2). Three distinct phylogenetic groups of winter oilseed rape isolates were detected using MLSA. Two genes, gltA and rpoD, showed the greatest ability to identify and discriminate winter oilseed rape Xcc isolates from isolates of the other six hosts. The lepA gene exhibited specific three-nucleotide changes in sequences of some of the isolates. Results of virulence testing of 18 representative isolates showed statistically significant host-pathogen specialization for Xcc isolates from winter oilseed rape, cauliflower, kale and kohlrabi. In conclusion, oilseed rape isolates are more genetically diverse and show greater specialization to their host in comparison to the rest of the tested isolates from other brassica hosts.
PB  - Wiley
T2  - Plant Pathology
T1  - Genetic diversity and virulence of Xanthomonas campestris pv. campestris isolates from Brassica napus and six Brassica oleracea crops in Serbia
EP  - 1458
IS  - 8
SP  - 1448
VL  - 68
DO  - 10.1111/ppa.13064
ER  - 

@article{
author = "Popović Milovanović, Tatjana and Mitrović, Petar and Jelušić, Aleksandra and Dimkić, Ivica and Marjanovic Jeromela, Ana and Nikolić, Ivan and Stanković, Slaviša",
year = "2019",
abstract = "The present study provides insight into the diversity of 147 Xanthomonas campestris pv. campestris (Xcc) isolates obtained from six Brassica oleracea vegetable crops (broccoli, cabbage, cauliflower, collard greens, kale, kohlrabi) and the winter oilseed rape crop Brassica napus, collected from different regions in Serbia in 2014. The XCF/XCR pathovar-specific primer set was used for fast preliminary identification. In repetitive sequence-based PCR (BOX, ERIC and REP) of all isolates, a higher level of genetic diversity was found in winter oilseed rape isolates compared to isolates from the other hosts. ERIC and REP-PCR showed the highest heterogeneity, with 10 and nine banding patterns, respectively. The REP-PCR results showed the highest correlation (70%) with those obtained with multilocus sequence analysis (MLSA), performed with 10 housekeeping genes (fusA, gap-1, gltA, gyrB1, lacF, lepA, rpoD, dnaK, fyuA and gyrB2). Three distinct phylogenetic groups of winter oilseed rape isolates were detected using MLSA. Two genes, gltA and rpoD, showed the greatest ability to identify and discriminate winter oilseed rape Xcc isolates from isolates of the other six hosts. The lepA gene exhibited specific three-nucleotide changes in sequences of some of the isolates. Results of virulence testing of 18 representative isolates showed statistically significant host-pathogen specialization for Xcc isolates from winter oilseed rape, cauliflower, kale and kohlrabi. In conclusion, oilseed rape isolates are more genetically diverse and show greater specialization to their host in comparison to the rest of the tested isolates from other brassica hosts.",
publisher = "Wiley",
journal = "Plant Pathology",
title = "Genetic diversity and virulence of Xanthomonas campestris pv. campestris isolates from Brassica napus and six Brassica oleracea crops in Serbia",
pages = "1458-1448",
number = "8",
volume = "68",
doi = "10.1111/ppa.13064"
}

Popović Milovanović, T., Mitrović, P., Jelušić, A., Dimkić, I., Marjanovic Jeromela, A., Nikolić, I.,& Stanković, S.. (2019). Genetic diversity and virulence of Xanthomonas campestris pv. campestris isolates from Brassica napus and six Brassica oleracea crops in Serbia. in Plant Pathology
Wiley., 68(8), 1448-1458.
https://doi.org/10.1111/ppa.13064

Popović Milovanović T, Mitrović P, Jelušić A, Dimkić I, Marjanovic Jeromela A, Nikolić I, Stanković S. Genetic diversity and virulence of Xanthomonas campestris pv. campestris isolates from Brassica napus and six Brassica oleracea crops in Serbia. in Plant Pathology. 2019;68(8):1448-1458.
doi:10.1111/ppa.13064 .

Popović Milovanović, Tatjana, Mitrović, Petar, Jelušić, Aleksandra, Dimkić, Ivica, Marjanovic Jeromela, Ana, Nikolić, Ivan, Stanković, Slaviša, "Genetic diversity and virulence of Xanthomonas campestris pv. campestris isolates from Brassica napus and six Brassica oleracea crops in Serbia" in Plant Pathology, 68, no. 8 (2019):1448-1458,
https://doi.org/10.1111/ppa.13064 . .