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PCR-RFLP-based method for reliable discrimination of cryptic species within Mecinus janthinus species complex (Mecinini, Curculionidae) introduced in North America for biological control of invasive toadflaxes
dc.creator | Toševski, Ivo | |
dc.creator | Jović, Jelena | |
dc.creator | Krstić, Oliver | |
dc.creator | Gassmann, Andre | |
dc.date.accessioned | 2019-04-04T15:59:22Z | |
dc.date.available | 2019-04-04T15:59:22Z | |
dc.date.issued | 2013 | |
dc.identifier.issn | 1386-6141 | |
dc.identifier.uri | https://plantarum.izbis.bg.ac.rs/handle/123456789/295 | |
dc.description.abstract | Several populations of the stem-mining weevil Mecinus janthinus Germar species complex (Mecinini, Curculionidae), identified based on morphological characteristics, have been introduced in North America for the biological control of invasive toadflaxes of European origin: Linaria vulgaris Miller and L. dalmatica (L.) Miller (Plantaginaceae). According to the mitochondrial cytochrome oxidase subunit II (COII) gene haplotype divergence of Mecinus janthinus species complex, a total of 20 M. janthinus s.s., 3 M. janthinus s.l. of the 'speciosa' genotype and 29 M. janthiniformis haplotypes have been recorded across their native range in central and southeastern Europe. A polymerase chain reaction followed by restriction fragment length polymorphism (PCR-RFLP) diagnostic assay of COII gene using Hpy188III and MnlI enzyme-mix, was developed for fast and cost-effective discrimination of these morphologically very similar cryptic weevil species. It is shown that digestion generates unique 4-fragment restriction profile in M. janthinus s.s., 2-fragment profile in M. janthiniformis and 3-fragment profile in M. janthinus s.l. 'speciosa' group of haplotypes, allowing precise identification of each species or genotype. The proposed method represents a practical tool for fast and accurate identification of the target biocontrol agents and should prevent using inappropriate weevil species in redistribution programs for biological control of invasive toadflax species. | en |
dc.publisher | Springer, Dordrecht | |
dc.relation | Ministry of Forests and Range, British Columbia Provincial Government | |
dc.relation | Wyoming Biological Control Steering Committee, USDA-APHIS-CPHST | |
dc.relation | USDA Forest Service and the Montana Noxious Weed Trust Fund through the Montana State University | |
dc.relation | California Department of Food and Agriculture | |
dc.relation | info:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/43001/RS// | |
dc.rights | restrictedAccess | |
dc.source | BioControl | |
dc.subject | Curculionidae | en |
dc.subject | Invasive toadflaxes | en |
dc.subject | Mecinus janthiniformis | en |
dc.subject | Mecinus janthinus | en |
dc.subject | Molecular identification of biocontrol agent | en |
dc.subject | PCR-RFLP diagnostic assay | en |
dc.title | PCR-RFLP-based method for reliable discrimination of cryptic species within Mecinus janthinus species complex (Mecinini, Curculionidae) introduced in North America for biological control of invasive toadflaxes | en |
dc.type | article | |
dc.rights.license | ARR | |
dc.citation.epage | 573 | |
dc.citation.issue | 4 | |
dc.citation.other | 58(4): 563-573 | |
dc.citation.rank | M21 | |
dc.citation.spage | 563 | |
dc.citation.volume | 58 | |
dc.type.version | publishedVersion | |
dc.identifier.doi | 10.1007/s10526-013-9506-2 | |
dc.identifier.scopus | 2-s2.0-84880508519 | |
dc.identifier.wos | 000323908400011 |