TY  - JOUR
AU  - Iličić, Renata
AU  - Jelušić, Aleksandra
AU  - Barać, Goran
AU  - Nikolić, Dušan
AU  - Stošić, Nemanja
AU  - Scortichini, Marco
AU  - Popović Milovanović, Tatjana
PY  - 2024
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/1245
AB  - In August 2020, the unusual appearance of crown gall symptoms was observed on the
tobacco plants (hybrid PVH2310) grown in fields in the Golubinci (Srem district, Serbia) locality. The
causal agent isolated from galls located on tobacco roots formed circular, convex, and glistening
light blue colonies, and then dark to olive-green-colored bacterial colonies on a semi-selective D1
medium. Molecular analysis based on multiplex PCR and multi-locus sequence analysis (MLSA)
using concatenated sequences of the atpD, dnaK, glnA, and rpoB genes as well as 16S rRNA identified
Serbian tobacco isolates such as Agrobacterium tumefaciens (biovar 1). Two duplex PCR methods
confirmed the presence of the virD2 and virC genes in tobacco isolates. Pathogenicity tests performed
on carrot discs and squash fruits resulted in tumor/gall formation after 12 to 16 days post inoculation,
respectively. Pathogenicity was also confirmed on tobacco plants, where isolates caused tumor
development 21−25 days after inoculation. API 50 CH generated results regarding the biochemical
features of the Serbian tobacco isolates. As A. tumefaciens (biovar 1) as a cause of tobacco crown gall
has previously been documented solely in Japan, there is presently no data on its wider occurrence.
Therefore, this first detailed investigation of A. tumefaciens isolated from naturally infected tobacco in
Serbia will contribute to a better understanding of it at the global level.
PB  - MDPI Basel
T2  - Agronomy
T1  - In-Depth Characterization of Crown Gall Disease of Tobacco in Serbia
IS  - 4
SP  - 851
VL  - 14
DO  - 10.3390/agronomy14040851
ER  - 

@article{
author = "Iličić, Renata and Jelušić, Aleksandra and Barać, Goran and Nikolić, Dušan and Stošić, Nemanja and Scortichini, Marco and Popović Milovanović, Tatjana",
year = "2024",
abstract = "In August 2020, the unusual appearance of crown gall symptoms was observed on the
tobacco plants (hybrid PVH2310) grown in fields in the Golubinci (Srem district, Serbia) locality. The
causal agent isolated from galls located on tobacco roots formed circular, convex, and glistening
light blue colonies, and then dark to olive-green-colored bacterial colonies on a semi-selective D1
medium. Molecular analysis based on multiplex PCR and multi-locus sequence analysis (MLSA)
using concatenated sequences of the atpD, dnaK, glnA, and rpoB genes as well as 16S rRNA identified
Serbian tobacco isolates such as Agrobacterium tumefaciens (biovar 1). Two duplex PCR methods
confirmed the presence of the virD2 and virC genes in tobacco isolates. Pathogenicity tests performed
on carrot discs and squash fruits resulted in tumor/gall formation after 12 to 16 days post inoculation,
respectively. Pathogenicity was also confirmed on tobacco plants, where isolates caused tumor
development 21−25 days after inoculation. API 50 CH generated results regarding the biochemical
features of the Serbian tobacco isolates. As A. tumefaciens (biovar 1) as a cause of tobacco crown gall
has previously been documented solely in Japan, there is presently no data on its wider occurrence.
Therefore, this first detailed investigation of A. tumefaciens isolated from naturally infected tobacco in
Serbia will contribute to a better understanding of it at the global level.",
publisher = "MDPI Basel",
journal = "Agronomy",
title = "In-Depth Characterization of Crown Gall Disease of Tobacco in Serbia",
number = "4",
pages = "851",
volume = "14",
doi = "10.3390/agronomy14040851"
}

Iličić, R., Jelušić, A., Barać, G., Nikolić, D., Stošić, N., Scortichini, M.,& Popović Milovanović, T.. (2024). In-Depth Characterization of Crown Gall Disease of Tobacco in Serbia. in Agronomy
MDPI Basel., 14(4), 851.
https://doi.org/10.3390/agronomy14040851

Iličić R, Jelušić A, Barać G, Nikolić D, Stošić N, Scortichini M, Popović Milovanović T. In-Depth Characterization of Crown Gall Disease of Tobacco in Serbia. in Agronomy. 2024;14(4):851.
doi:10.3390/agronomy14040851 .

Iličić, Renata, Jelušić, Aleksandra, Barać, Goran, Nikolić, Dušan, Stošić, Nemanja, Scortichini, Marco, Popović Milovanović, Tatjana, "In-Depth Characterization of Crown Gall Disease of Tobacco in Serbia" in Agronomy, 14, no. 4 (2024):851,
https://doi.org/10.3390/agronomy14040851 . .

TY  - JOUR
AU  - Tomić, Andrija
AU  - Trkulja, Vojislav
AU  - Matić, Slavica
AU  - Trkulja, Nenad
AU  - Iličić, Renata
AU  - Scortichini, Marco
AU  - Popović Milovanović, Tatjana
PY  - 2024
UR  - https://enauka.gov.rs/handle/123456789/897621
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/1233
AB  - Pyrenophora teres is a pathogen causing a net blotch disease in cultivated barley, which is present worldwide
and can thus significantly reduce barley yields. This fungus also infects wild barley and other plants of the Hordeum
genus, as well as barley grass, wheat, oats and plants from various genera, including Agropyron, Bromus, Elymus, Hordelymus and Stipa. Based on the symptoms it causes on the infected barley plants, the pathogen can be divided into two
forms: P. teres f. teres, which causes net-like symptoms, and P. teres f. maculata, which causes blotchy symptoms. Infected seeds, stubble and plant debris, and volunteer and weed plants represent primary sources of pathogen inoculum.
During the growing season, the pathogen enters a sexual stage, developing pseudothecia with asci and ascospores. This
is followed by an asexual stage, during which conidiophores with conidia are formed. The conidial (anamorphic) stage is
much more common, whereby conidia is a source of inoculum for secondary infection during the barley growing season.
The first symptoms appear at the end of winter and the beginning of spring, often during the tilling phase. The most
characteristic symptoms form on barley leaves. Frequently, symptoms of the net form can be mistaken for other diseases
occurring on barley, making molecular analysis essential for accurate detection of P. teres, its forms, mating types and
hybrids. Current net blotch control measures are based on the combined application of cultural, chemical and biological
control methods and the selection of resistant varieties.
PB  - Czech Academy of Agricultural Sciences
T2  - Plant Protection Science
T1  - Net blotch (Pyrenophora teres Drechsler): An increasingly significant threat to barley production
EP  - 30
IS  - 1
SP  - 1
VL  - 60
DO  - 10.17221/122/2023-PPS
ER  - 

@article{
author = "Tomić, Andrija and Trkulja, Vojislav and Matić, Slavica and Trkulja, Nenad and Iličić, Renata and Scortichini, Marco and Popović Milovanović, Tatjana",
year = "2024",
abstract = "Pyrenophora teres is a pathogen causing a net blotch disease in cultivated barley, which is present worldwide
and can thus significantly reduce barley yields. This fungus also infects wild barley and other plants of the Hordeum
genus, as well as barley grass, wheat, oats and plants from various genera, including Agropyron, Bromus, Elymus, Hordelymus and Stipa. Based on the symptoms it causes on the infected barley plants, the pathogen can be divided into two
forms: P. teres f. teres, which causes net-like symptoms, and P. teres f. maculata, which causes blotchy symptoms. Infected seeds, stubble and plant debris, and volunteer and weed plants represent primary sources of pathogen inoculum.
During the growing season, the pathogen enters a sexual stage, developing pseudothecia with asci and ascospores. This
is followed by an asexual stage, during which conidiophores with conidia are formed. The conidial (anamorphic) stage is
much more common, whereby conidia is a source of inoculum for secondary infection during the barley growing season.
The first symptoms appear at the end of winter and the beginning of spring, often during the tilling phase. The most
characteristic symptoms form on barley leaves. Frequently, symptoms of the net form can be mistaken for other diseases
occurring on barley, making molecular analysis essential for accurate detection of P. teres, its forms, mating types and
hybrids. Current net blotch control measures are based on the combined application of cultural, chemical and biological
control methods and the selection of resistant varieties.",
publisher = "Czech Academy of Agricultural Sciences",
journal = "Plant Protection Science",
title = "Net blotch (Pyrenophora teres Drechsler): An increasingly significant threat to barley production",
pages = "30-1",
number = "1",
volume = "60",
doi = "10.17221/122/2023-PPS"
}

Tomić, A., Trkulja, V., Matić, S., Trkulja, N., Iličić, R., Scortichini, M.,& Popović Milovanović, T.. (2024). Net blotch (Pyrenophora teres Drechsler): An increasingly significant threat to barley production. in Plant Protection Science
Czech Academy of Agricultural Sciences., 60(1), 1-30.
https://doi.org/10.17221/122/2023-PPS

Tomić A, Trkulja V, Matić S, Trkulja N, Iličić R, Scortichini M, Popović Milovanović T. Net blotch (Pyrenophora teres Drechsler): An increasingly significant threat to barley production. in Plant Protection Science. 2024;60(1):1-30.
doi:10.17221/122/2023-PPS .

Tomić, Andrija, Trkulja, Vojislav, Matić, Slavica, Trkulja, Nenad, Iličić, Renata, Scortichini, Marco, Popović Milovanović, Tatjana, "Net blotch (Pyrenophora teres Drechsler): An increasingly significant threat to barley production" in Plant Protection Science, 60, no. 1 (2024):1-30,
https://doi.org/10.17221/122/2023-PPS . .

TY  - JOUR
AU  - Bagi, Ferenc
AU  - Iličić, Renata
AU  - Konstantin, Đina
AU  - Pavković, Nemanja
AU  - Milovanović, Predrag
AU  - Popović Milovanović, Tatjana
PY  - 2024
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/1238
AB  - Species of the genus Alternaria are significant wheat contaminants
during production, transport and storage, requiring biocontrol measures which typically rely on
the bacteria from the Bacillus genera. As these are among the most beneficial and exploited
biocontrol agents, in this study, the inhibitory activity of indigenous Bacillus spp. was assessed
against the Alternaria alternata isolate originating from the wheat seed. Two of the
fifteen Bacillus s pp. i ncluded i n t he s tudy s howed t he i nhibitory effect. Specifically,
25.0−55.0% inhibition of A. alternata growth was achieved when the isolate coded as NB11
was applied in 106−109 cells mL-1 concentrations. On the other hand, when applied in 107−109
cells mL-1 concentrations, the isolate coded as NB16 inhibited A. alternata growth by
35.2−51.1%, but was ineffective at lower concentrations. Thus, these in vitro assays indicate
that both Bacillus spp. (NB11 and NB16) isolated from the wheat rhizosphere can be applied
in practice in the control of A. alternata.
PB  - Matica srpska , Serbia
T2  - MATICA SRPSKA JOURNAL FOR NATURAL SCIENCES
T1  - УТИЦАЈ Bacillus spp. НА ПАТОГЕНА ПШЕНИЦЕ Alternaria alternata И  in vitro УСЛОВИМА
EP  - 71
SP  - 7
VL  - 146
ER  - 

@article{
author = "Bagi, Ferenc and Iličić, Renata and Konstantin, Đina and Pavković, Nemanja and Milovanović, Predrag and Popović Milovanović, Tatjana",
year = "2024",
abstract = "Species of the genus Alternaria are significant wheat contaminants
during production, transport and storage, requiring biocontrol measures which typically rely on
the bacteria from the Bacillus genera. As these are among the most beneficial and exploited
biocontrol agents, in this study, the inhibitory activity of indigenous Bacillus spp. was assessed
against the Alternaria alternata isolate originating from the wheat seed. Two of the
fifteen Bacillus s pp. i ncluded i n t he s tudy s howed t he i nhibitory effect. Specifically,
25.0−55.0% inhibition of A. alternata growth was achieved when the isolate coded as NB11
was applied in 106−109 cells mL-1 concentrations. On the other hand, when applied in 107−109
cells mL-1 concentrations, the isolate coded as NB16 inhibited A. alternata growth by
35.2−51.1%, but was ineffective at lower concentrations. Thus, these in vitro assays indicate
that both Bacillus spp. (NB11 and NB16) isolated from the wheat rhizosphere can be applied
in practice in the control of A. alternata.",
publisher = "Matica srpska , Serbia",
journal = "MATICA SRPSKA JOURNAL FOR NATURAL SCIENCES",
title = "УТИЦАЈ Bacillus spp. НА ПАТОГЕНА ПШЕНИЦЕ Alternaria alternata И  in vitro УСЛОВИМА",
pages = "71-7",
volume = "146"
}

Bagi, F., Iličić, R., Konstantin, Đ., Pavković, N., Milovanović, P.,& Popović Milovanović, T.. (2024). УТИЦАЈ Bacillus spp. НА ПАТОГЕНА ПШЕНИЦЕ Alternaria alternata И  in vitro УСЛОВИМА. in MATICA SRPSKA JOURNAL FOR NATURAL SCIENCES
Matica srpska , Serbia., 146, 7-71.

Bagi F, Iličić R, Konstantin Đ, Pavković N, Milovanović P, Popović Milovanović T. УТИЦАЈ Bacillus spp. НА ПАТОГЕНА ПШЕНИЦЕ Alternaria alternata И  in vitro УСЛОВИМА. in MATICA SRPSKA JOURNAL FOR NATURAL SCIENCES. 2024;146:7-71..

Bagi, Ferenc, Iličić, Renata, Konstantin, Đina, Pavković, Nemanja, Milovanović, Predrag, Popović Milovanović, Tatjana, "УТИЦАЈ Bacillus spp. НА ПАТОГЕНА ПШЕНИЦЕ Alternaria alternata И  in vitro УСЛОВИМА" in MATICA SRPSKA JOURNAL FOR NATURAL SCIENCES, 146 (2024):7-71.

TY  - CHAP
AU  - Trkulja, Vojislav
AU  - Tomić, Andrija
AU  - Popović Milovanović, Tatjana
AU  - Iličić, Renata
PY  - 2023
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/1212
AB  - Uticaji klimatskih promjena na štetne organizme su složeni iz razloga što i drugi faktori koji utiču na njih nisu konstantni već se mijenjaju. I pored toga, klimatske promjene mogu na slične ili različite načine uticati na razne prouzrokovače biljnih bolesti i štetočine, u zavisnosti kojoj grupi organizama oni pripadaju: gljivama, bakterijama, virusima, insektima, nematodama i slično. Кod fitopatogenih gljiva ovi uticaji mogu se ispoljiti u pogledu: promjene biologije; brže evolucije zbog dužeg godišnjeg doba; pojave novih rasa ili veće agresivnosti postojećih vrsta zbog rekombinacije gena; mijenjanja geografske rasprostranjenosti, bilo prema sjevernoj hemisferi ili na područja sa višom nadmorskom visinom; introdukcije karantinskih i invazivnih vrsta, kao i širenja u nova područja u odnosu na područje porijekla; veću produkciju mikotoksina i dr. Za viruse i bakterije koji se prenose vektorima od posebnog značaja je uticaj klimatskih promjena na prisustvo, širenje i brojnost vektora.
Pozitivan uticaj klimatskih promjena na različite štetočine biljaka može se ispoljiti u vidu: promjene biologije i pojave većeg broja generacija; povećane brojnosti i plodnosti; boljeg prezimljavanja; proširenog kruga domaćina; introdukcije karantinskih i invazivnih vrsta; širenja u nova područja i drugo. Pozitivni uticaji klimatskih promjena na štetne organizme najčešće su sa negativnim efektom na razvoj poljoprivrede i proizvodnju hrane, razvoj šumarstva, biodiverzitet i životnu sredinu – zbog mogućnosti nastanka većih ekonomskih šteta, kao i većih potreba za primjenom pesticida. Кlimatske promjene, takođe, mogu uticati i na biljke domaćine, pri čemu je od posebnog značaja njihov gubitak prirodne osnove otpornosti.
Iako je došlo do napretka u praćenju i razumijevanju klimatskih promjena, ostaju potrebe za mnogim naučnim, tehničkim i institucionalnim rješenjima za precizno planiranje, prilagođavanje i ublažavanje efekata klimatskih promjena na prouzrokovače biljnih bolesti i štetočine, kao i biljke domaćine i njihove interakcije.
PB  - Akademija nauka i umjetnosti Republike Srpske, Banja Luka
T2  - Trbić G., Popov T., Mirjanić D. Upravljanje prirodnim resursima u eri klimatskih promjena
T1  - Uticaj klimatskih promjena na pojavu bolesti štetočina poljoprivrednih biljaka i šumskog drveća
EP  - 517
SP  - 477
DO  - 10,7251/EORU2308477T
ER  - 

@inbook{
author = "Trkulja, Vojislav and Tomić, Andrija and Popović Milovanović, Tatjana and Iličić, Renata",
year = "2023",
abstract = "Uticaji klimatskih promjena na štetne organizme su složeni iz razloga što i drugi faktori koji utiču na njih nisu konstantni već se mijenjaju. I pored toga, klimatske promjene mogu na slične ili različite načine uticati na razne prouzrokovače biljnih bolesti i štetočine, u zavisnosti kojoj grupi organizama oni pripadaju: gljivama, bakterijama, virusima, insektima, nematodama i slično. Кod fitopatogenih gljiva ovi uticaji mogu se ispoljiti u pogledu: promjene biologije; brže evolucije zbog dužeg godišnjeg doba; pojave novih rasa ili veće agresivnosti postojećih vrsta zbog rekombinacije gena; mijenjanja geografske rasprostranjenosti, bilo prema sjevernoj hemisferi ili na područja sa višom nadmorskom visinom; introdukcije karantinskih i invazivnih vrsta, kao i širenja u nova područja u odnosu na područje porijekla; veću produkciju mikotoksina i dr. Za viruse i bakterije koji se prenose vektorima od posebnog značaja je uticaj klimatskih promjena na prisustvo, širenje i brojnost vektora.
Pozitivan uticaj klimatskih promjena na različite štetočine biljaka može se ispoljiti u vidu: promjene biologije i pojave većeg broja generacija; povećane brojnosti i plodnosti; boljeg prezimljavanja; proširenog kruga domaćina; introdukcije karantinskih i invazivnih vrsta; širenja u nova područja i drugo. Pozitivni uticaji klimatskih promjena na štetne organizme najčešće su sa negativnim efektom na razvoj poljoprivrede i proizvodnju hrane, razvoj šumarstva, biodiverzitet i životnu sredinu – zbog mogućnosti nastanka većih ekonomskih šteta, kao i većih potreba za primjenom pesticida. Кlimatske promjene, takođe, mogu uticati i na biljke domaćine, pri čemu je od posebnog značaja njihov gubitak prirodne osnove otpornosti.
Iako je došlo do napretka u praćenju i razumijevanju klimatskih promjena, ostaju potrebe za mnogim naučnim, tehničkim i institucionalnim rješenjima za precizno planiranje, prilagođavanje i ublažavanje efekata klimatskih promjena na prouzrokovače biljnih bolesti i štetočine, kao i biljke domaćine i njihove interakcije.",
publisher = "Akademija nauka i umjetnosti Republike Srpske, Banja Luka",
journal = "Trbić G., Popov T., Mirjanić D. Upravljanje prirodnim resursima u eri klimatskih promjena",
booktitle = "Uticaj klimatskih promjena na pojavu bolesti štetočina poljoprivrednih biljaka i šumskog drveća",
pages = "517-477",
doi = "10,7251/EORU2308477T"
}

Trkulja, V., Tomić, A., Popović Milovanović, T.,& Iličić, R.. (2023). Uticaj klimatskih promjena na pojavu bolesti štetočina poljoprivrednih biljaka i šumskog drveća. in Trbić G., Popov T., Mirjanić D. Upravljanje prirodnim resursima u eri klimatskih promjena
Akademija nauka i umjetnosti Republike Srpske, Banja Luka., 477-517.
https://doi.org/10,7251/EORU2308477T

Trkulja V, Tomić A, Popović Milovanović T, Iličić R. Uticaj klimatskih promjena na pojavu bolesti štetočina poljoprivrednih biljaka i šumskog drveća. in Trbić G., Popov T., Mirjanić D. Upravljanje prirodnim resursima u eri klimatskih promjena. 2023;:477-517.
doi:10,7251/EORU2308477T .

Trkulja, Vojislav, Tomić, Andrija, Popović Milovanović, Tatjana, Iličić, Renata, "Uticaj klimatskih promjena na pojavu bolesti štetočina poljoprivrednih biljaka i šumskog drveća" in Trbić G., Popov T., Mirjanić D. Upravljanje prirodnim resursima u eri klimatskih promjena (2023):477-517,
https://doi.org/10,7251/EORU2308477T . .

TY  - CONF
AU  - Popović Milovanović, Tatjana
AU  - Iličić, Renata
AU  - Trkulja, Nenad
AU  - Trkulja, Vojislav
AU  - Zečević, Katarina
AU  - Jelušić, Aleksandra
PY  - 2023
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/1196
AB  - Bakteriozna mrljavost plodova lubenice (Acidovorax citrulli) predstavlja širom sveta
ozbiljnu pretnju uključujući uzgajivače i proizvođače semena i rasada gajenih vrsta biljaka iz
porodice Cucurbitacae. Krajem 1980-ih, bakterija A. citrulli se nakon epidemijskih pojava na
lubenici u SAD proširila na druga područja i useve (dinja, tikva, bundeva, krastavac). Do danas
su opisane dve genetički različite populacije A. citrulli. U Srbiji je prvi nalaz A. citrulli
zabeležen 2014. godine na lubenici u lokalitetima Bačke i Srema. Međutim, uprkos
sprovedenim eradikativnim merama i naporima u upravljanju ovim patogenom, ponovo je
detektovan 2018., 2021., i 2022. godine na području Mačve i Srema. U cilju boljeg
razumevanja genetičke strukture populacija A. citrulli prisutnih u Srbiji, u ovom radu je
korišćena analiza sekvenci više genskih lokusa (MLSA) primenom konzerviranih gena i gena
virulentnosti.
Tokom rada korišćena su četiri reprezentativna soja A. citrulli, pod šiframa Ac414
(Čelarevo, 2014), Ac818 (Šabac, 2018), Ac221 (Ašanja, 2021) i Ac322 (Hrtkovci, 2022),
odabrana na osnovu prethodnih rezultata dobijenih umnožavanjem ponavljajućih sekvenci
DNK (rep-PCR). Za MLSA je korišćeno devet gena, i to osam konzervativnih (gltA, gmc, gyrB,
lepA, phaC, pilT, trpB, ugpB) i jedan gen virulentnosti (Aave_1548). PCR program se sastojao
od inicijalne denaturacije (5 min na 95 °C), praćene sa 30 ciklusa denaturacije (30 s na 95 °C),
11
hibridizacije (30 s na 60 °C za konzervativne gene i 55 °C za gen virulentnosti Aave_1548) i
elongacije (30 s za konzervativne gene i 90 s za gen Aave_1548 na 72 °C); i finalne elongacije
(5 min na 72 °C). Amplifikovani produkti su sekvencirani u Eurofins Genomics (Hamburg,
Nemačka). Kvalitet dobijenih sekvenci je manuelno pregledan, nakon čega je za svaki od četiri
testirana soja napravljena multigenska sekvenca na osnovu svih sekvenciranih gena.
mMultigenske sekvence testiranih i referentnih sojeva A. citrulli preuzetih iz baze podataka
Nacionalnog centra za biotehnološke informacije (NCBI) korišćene su za konstruisanje
„Neighbor-joining” filogenetskog stabla. Na osnovu rezultata filogenetske analize, dokazano je
da sojevi A. citrulli izolovani iz prirodno zaraženih plodova lubenice u Srbiji (2014-2022)
pripadaju dvema reprezentativnim genetičkim grupama opisanim u svetu (grupe I i II). Grupi
I su pripadali sojevi izolovani 2021. i 2022. godine, a grupi II sojevi izolovani 2014. i 2018.
godine. Rezultati dobijeni u ovom radu ukazuju na puteve introdukcije i širenja A. citrulli u
Srbiji.
PB  - Društvo za zaštitu bilja
C3  - XVII simpozijum o zaštiti bilja, Zlatibor 2023, 27-30. novembar, Zbornik rezimea radova
T1  - Utvrđivanje genetičke strukture populacija Acidovorax citrulli u Srbiji
EP  - 11
SP  - 10
ER  - 

@conference{
author = "Popović Milovanović, Tatjana and Iličić, Renata and Trkulja, Nenad and Trkulja, Vojislav and Zečević, Katarina and Jelušić, Aleksandra",
year = "2023",
abstract = "Bakteriozna mrljavost plodova lubenice (Acidovorax citrulli) predstavlja širom sveta
ozbiljnu pretnju uključujući uzgajivače i proizvođače semena i rasada gajenih vrsta biljaka iz
porodice Cucurbitacae. Krajem 1980-ih, bakterija A. citrulli se nakon epidemijskih pojava na
lubenici u SAD proširila na druga područja i useve (dinja, tikva, bundeva, krastavac). Do danas
su opisane dve genetički različite populacije A. citrulli. U Srbiji je prvi nalaz A. citrulli
zabeležen 2014. godine na lubenici u lokalitetima Bačke i Srema. Međutim, uprkos
sprovedenim eradikativnim merama i naporima u upravljanju ovim patogenom, ponovo je
detektovan 2018., 2021., i 2022. godine na području Mačve i Srema. U cilju boljeg
razumevanja genetičke strukture populacija A. citrulli prisutnih u Srbiji, u ovom radu je
korišćena analiza sekvenci više genskih lokusa (MLSA) primenom konzerviranih gena i gena
virulentnosti.
Tokom rada korišćena su četiri reprezentativna soja A. citrulli, pod šiframa Ac414
(Čelarevo, 2014), Ac818 (Šabac, 2018), Ac221 (Ašanja, 2021) i Ac322 (Hrtkovci, 2022),
odabrana na osnovu prethodnih rezultata dobijenih umnožavanjem ponavljajućih sekvenci
DNK (rep-PCR). Za MLSA je korišćeno devet gena, i to osam konzervativnih (gltA, gmc, gyrB,
lepA, phaC, pilT, trpB, ugpB) i jedan gen virulentnosti (Aave_1548). PCR program se sastojao
od inicijalne denaturacije (5 min na 95 °C), praćene sa 30 ciklusa denaturacije (30 s na 95 °C),
11
hibridizacije (30 s na 60 °C za konzervativne gene i 55 °C za gen virulentnosti Aave_1548) i
elongacije (30 s za konzervativne gene i 90 s za gen Aave_1548 na 72 °C); i finalne elongacije
(5 min na 72 °C). Amplifikovani produkti su sekvencirani u Eurofins Genomics (Hamburg,
Nemačka). Kvalitet dobijenih sekvenci je manuelno pregledan, nakon čega je za svaki od četiri
testirana soja napravljena multigenska sekvenca na osnovu svih sekvenciranih gena.
mMultigenske sekvence testiranih i referentnih sojeva A. citrulli preuzetih iz baze podataka
Nacionalnog centra za biotehnološke informacije (NCBI) korišćene su za konstruisanje
„Neighbor-joining” filogenetskog stabla. Na osnovu rezultata filogenetske analize, dokazano je
da sojevi A. citrulli izolovani iz prirodno zaraženih plodova lubenice u Srbiji (2014-2022)
pripadaju dvema reprezentativnim genetičkim grupama opisanim u svetu (grupe I i II). Grupi
I su pripadali sojevi izolovani 2021. i 2022. godine, a grupi II sojevi izolovani 2014. i 2018.
godine. Rezultati dobijeni u ovom radu ukazuju na puteve introdukcije i širenja A. citrulli u
Srbiji.",
publisher = "Društvo za zaštitu bilja",
journal = "XVII simpozijum o zaštiti bilja, Zlatibor 2023, 27-30. novembar, Zbornik rezimea radova",
title = "Utvrđivanje genetičke strukture populacija Acidovorax citrulli u Srbiji",
pages = "11-10"
}

Popović Milovanović, T., Iličić, R., Trkulja, N., Trkulja, V., Zečević, K.,& Jelušić, A.. (2023). Utvrđivanje genetičke strukture populacija Acidovorax citrulli u Srbiji. in XVII simpozijum o zaštiti bilja, Zlatibor 2023, 27-30. novembar, Zbornik rezimea radova
Društvo za zaštitu bilja., 10-11.

Popović Milovanović T, Iličić R, Trkulja N, Trkulja V, Zečević K, Jelušić A. Utvrđivanje genetičke strukture populacija Acidovorax citrulli u Srbiji. in XVII simpozijum o zaštiti bilja, Zlatibor 2023, 27-30. novembar, Zbornik rezimea radova. 2023;:10-11..

Popović Milovanović, Tatjana, Iličić, Renata, Trkulja, Nenad, Trkulja, Vojislav, Zečević, Katarina, Jelušić, Aleksandra, "Utvrđivanje genetičke strukture populacija Acidovorax citrulli u Srbiji" in XVII simpozijum o zaštiti bilja, Zlatibor 2023, 27-30. novembar, Zbornik rezimea radova (2023):10-11.

Ivić, D., Novak, A., Plavec, J., Iličić, R.,& Popović Milovanović, T.. (2023). 2023 First Report of Pseudomonas mediterranea Causing Tomato Pith Necrosis in Croatia. in Plant Disease
Scientific Societies., 107(7), 2217.
https://doi.org/10.1094/PDIS-10-22-2371-PDN

Ivić D, Novak A, Plavec J, Iličić R, Popović Milovanović T. 2023 First Report of Pseudomonas mediterranea Causing Tomato Pith Necrosis in Croatia. in Plant Disease. 2023;107(7):2217.
doi:10.1094/PDIS-10-22-2371-PDN .

Ivić, Dario, Novak, Adrijana, Plavec, Jelena, Iličić, Renata, Popović Milovanović, Tatjana, "2023 First Report of Pseudomonas mediterranea Causing Tomato Pith Necrosis in Croatia" in Plant Disease, 107, no. 7 (2023):2217,
https://doi.org/10.1094/PDIS-10-22-2371-PDN . .

Popović Milovanović, T., Milićević, Z., Krnjajić, S., Milovanović, P., Iličić, R.,& Zečević, K.. (2023). Nova formulacija ulja karanfilića (Syzygium aromaticum L.) u kontroli brojnosti krompirovog moljca (Phthorimaea operculella). in Institut za zaštitu bilja i životnu sredinu
Institut za zaštitu bilja i životnu sredinu, Beograd..

Popović Milovanović T, Milićević Z, Krnjajić S, Milovanović P, Iličić R, Zečević K. Nova formulacija ulja karanfilića (Syzygium aromaticum L.) u kontroli brojnosti krompirovog moljca (Phthorimaea operculella). in Institut za zaštitu bilja i životnu sredinu. 2023;..

Popović Milovanović, Tatjana, Milićević, Zoran, Krnjajić, Slobodan, Milovanović, Predrag, Iličić, Renata, Zečević, Katarina, "Nova formulacija ulja karanfilića (Syzygium aromaticum L.) u kontroli brojnosti krompirovog moljca (Phthorimaea operculella)" in Institut za zaštitu bilja i životnu sredinu (2023).

TY  - JOUR
AU  - Jelušić, Aleksandra
AU  - Mitrović, Petar
AU  - Iličić, Renata
AU  - Milovanović, Predrag
AU  - Stanković, Slaviša
AU  - Popović Milovanović, Tatjana
PY  - 2023
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/1137
AB  - The aim of this work was to identify and characterize the pectolytic bacteria responsible for the emergence of bacterial soft rot on two summer cabbage hybrids (Cheers F1 and Hippo F1) grown in the Futog locality (Bačka, Vojvodina), known for the five-century-long tradition of cabbage cultivation in Serbia. Symptoms manifesting as soft lesions on outer head leaves were observed during August 2021, while the inner tissues were macerated, featuring cream to black discoloration. As the affected tissue decomposed, it exuded a specific odor. Disease incidence ranged from 15% to 25%. A total of 67 isolates producing pits on crystal violet pectate (CVP) medium were characterized for their phenotypic and genotypic features. The pathogenicity was confirmed on cabbage heads. Findings yielded by the repetitive element palindromic-polymerase chain reaction (rep-PCR) technique confirmed interspecies diversity between cabbage isolates, as well as intraspecies genetic diversity within the P. carotovorum group of isolates. Based on multilocus sequence typing (MLST) using genes dnaX, mdh, icdA, and proA, five representative isolates were identified as Pectobacterium carotovorum (Cheers F1 and Hippo F1), while two were identified as Pectobacterium versatile (Hippo F1) and Pectobacterium odoriferum (Hippo F1), respectively, indicating the presence of diverse Pectobacterium species even in combined infection in the same field. Among the obtained isolates, P. carotovorum was the most prevalent species (62.69%), while P. versatile and P. odoriferum were less represented (contributing by 19.40% and 17.91%, respectively). Multilocus sequence analysis (MLSA) performed with concatenated sequences of four housekeeping genes (proA, dnaX, icdA, and mdh) and constructed a neighbor-joining phylogenetic tree enabled insight into the phylogenetic position of the Serbian cabbage Pectobacterium isolates. Bacterium P. odoriferum was found to be the most virulent species for cabbage, followed by P. versatile, while all three species had comparable virulence with respect to potato. The results obtained in this work provide a better understanding of the spreading routes and abundance of different Pectobacterium spp. in Serbia.
PB  - MDPI
T2  - Microorganisms
T1  - Diversity of Bacterial Soft Rot-Causing Pectobacterium Species Affecting Cabbage in Serbia
IS  - 2
SP  - 335
VL  - 11
DO  - 10.3390/microorganisms11020335
ER  - 

@article{
author = "Jelušić, Aleksandra and Mitrović, Petar and Iličić, Renata and Milovanović, Predrag and Stanković, Slaviša and Popović Milovanović, Tatjana",
year = "2023",
abstract = "The aim of this work was to identify and characterize the pectolytic bacteria responsible for the emergence of bacterial soft rot on two summer cabbage hybrids (Cheers F1 and Hippo F1) grown in the Futog locality (Bačka, Vojvodina), known for the five-century-long tradition of cabbage cultivation in Serbia. Symptoms manifesting as soft lesions on outer head leaves were observed during August 2021, while the inner tissues were macerated, featuring cream to black discoloration. As the affected tissue decomposed, it exuded a specific odor. Disease incidence ranged from 15% to 25%. A total of 67 isolates producing pits on crystal violet pectate (CVP) medium were characterized for their phenotypic and genotypic features. The pathogenicity was confirmed on cabbage heads. Findings yielded by the repetitive element palindromic-polymerase chain reaction (rep-PCR) technique confirmed interspecies diversity between cabbage isolates, as well as intraspecies genetic diversity within the P. carotovorum group of isolates. Based on multilocus sequence typing (MLST) using genes dnaX, mdh, icdA, and proA, five representative isolates were identified as Pectobacterium carotovorum (Cheers F1 and Hippo F1), while two were identified as Pectobacterium versatile (Hippo F1) and Pectobacterium odoriferum (Hippo F1), respectively, indicating the presence of diverse Pectobacterium species even in combined infection in the same field. Among the obtained isolates, P. carotovorum was the most prevalent species (62.69%), while P. versatile and P. odoriferum were less represented (contributing by 19.40% and 17.91%, respectively). Multilocus sequence analysis (MLSA) performed with concatenated sequences of four housekeeping genes (proA, dnaX, icdA, and mdh) and constructed a neighbor-joining phylogenetic tree enabled insight into the phylogenetic position of the Serbian cabbage Pectobacterium isolates. Bacterium P. odoriferum was found to be the most virulent species for cabbage, followed by P. versatile, while all three species had comparable virulence with respect to potato. The results obtained in this work provide a better understanding of the spreading routes and abundance of different Pectobacterium spp. in Serbia.",
publisher = "MDPI",
journal = "Microorganisms",
title = "Diversity of Bacterial Soft Rot-Causing Pectobacterium Species Affecting Cabbage in Serbia",
number = "2",
pages = "335",
volume = "11",
doi = "10.3390/microorganisms11020335"
}

Jelušić, A., Mitrović, P., Iličić, R., Milovanović, P., Stanković, S.,& Popović Milovanović, T.. (2023). Diversity of Bacterial Soft Rot-Causing Pectobacterium Species Affecting Cabbage in Serbia. in Microorganisms
MDPI., 11(2), 335.
https://doi.org/10.3390/microorganisms11020335

Jelušić A, Mitrović P, Iličić R, Milovanović P, Stanković S, Popović Milovanović T. Diversity of Bacterial Soft Rot-Causing Pectobacterium Species Affecting Cabbage in Serbia. in Microorganisms. 2023;11(2):335.
doi:10.3390/microorganisms11020335 .

Jelušić, Aleksandra, Mitrović, Petar, Iličić, Renata, Milovanović, Predrag, Stanković, Slaviša, Popović Milovanović, Tatjana, "Diversity of Bacterial Soft Rot-Causing Pectobacterium Species Affecting Cabbage in Serbia" in Microorganisms, 11, no. 2 (2023):335,
https://doi.org/10.3390/microorganisms11020335 . .

TY  - JOUR
AU  - Iličić, Renata
AU  - Jelušić, Aleksandra
AU  - Blagojević, Milan
AU  - Bagi, Ferenc
AU  - Vrandečić, Karolina
AU  - Stanković, Slaviša
AU  - Popović Milovanović, Tatjana
PY  - 2023
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/875
AB  - Pseudomonas strains originating from symptomatic (bacterial spot) leaf tissues of sweet cherry (Topola, Šumadija) and plum (Krušedol Selo, Srem) were isolated during 2016 and 2020, respectively. Based on the findings yielded by classical microbiological methods, LOPAT (+---+), GATTa (--++) and pathogenicity tests performed on detached fruitlets (sweet and sour cherry) and pods (bean pods), all strains were confirmed to belong to P. syringae pv. morsprunorum. The detection of cfl gene allowed strains that belong to race 1 to be identified. The DNA fingerprinting patterns obtained with four rep-PCR (BOX and ERIC), RAPD-PCR (M13), and IS50-PCR (IS50) methods revealed that the seven tested sweet cherry and plum P. s. pv. morsprunorum strains, as well as comparative KBNS71 and the reference strain CFBP 2119, were genetically heterogeneous. Conversely, MLSA based on the four-gene-based scheme (gapA, gltA, gyrB, and rpoD) indicated genetic homogeneity among all tested Serbian sweet cherry and plum strains, as well as P. s. pv. morsprunorum race 1 strains from the NCBI. Although the MLSA findings indicate that the sweet cherry and plum strains used in this study are 100% identical, as they might have different virulence genes, genome sequencing should be performed to eventually find the strain sub-clades based on the host.
PB  - Serbian Genetics Society
T2  - Genetika
T1  - Characterization of Pseudomonas syringae pv. morsprunorum originating from sweet cherry and plum in Serbia
EP  - 176
IS  - 1
SP  - 159
VL  - 55
DO  - 10.2298/gensr2301159i
ER  - 

@article{
author = "Iličić, Renata and Jelušić, Aleksandra and Blagojević, Milan and Bagi, Ferenc and Vrandečić, Karolina and Stanković, Slaviša and Popović Milovanović, Tatjana",
year = "2023",
abstract = "Pseudomonas strains originating from symptomatic (bacterial spot) leaf tissues of sweet cherry (Topola, Šumadija) and plum (Krušedol Selo, Srem) were isolated during 2016 and 2020, respectively. Based on the findings yielded by classical microbiological methods, LOPAT (+---+), GATTa (--++) and pathogenicity tests performed on detached fruitlets (sweet and sour cherry) and pods (bean pods), all strains were confirmed to belong to P. syringae pv. morsprunorum. The detection of cfl gene allowed strains that belong to race 1 to be identified. The DNA fingerprinting patterns obtained with four rep-PCR (BOX and ERIC), RAPD-PCR (M13), and IS50-PCR (IS50) methods revealed that the seven tested sweet cherry and plum P. s. pv. morsprunorum strains, as well as comparative KBNS71 and the reference strain CFBP 2119, were genetically heterogeneous. Conversely, MLSA based on the four-gene-based scheme (gapA, gltA, gyrB, and rpoD) indicated genetic homogeneity among all tested Serbian sweet cherry and plum strains, as well as P. s. pv. morsprunorum race 1 strains from the NCBI. Although the MLSA findings indicate that the sweet cherry and plum strains used in this study are 100% identical, as they might have different virulence genes, genome sequencing should be performed to eventually find the strain sub-clades based on the host.",
publisher = "Serbian Genetics Society",
journal = "Genetika",
title = "Characterization of Pseudomonas syringae pv. morsprunorum originating from sweet cherry and plum in Serbia",
pages = "176-159",
number = "1",
volume = "55",
doi = "10.2298/gensr2301159i"
}

Iličić, R., Jelušić, A., Blagojević, M., Bagi, F., Vrandečić, K., Stanković, S.,& Popović Milovanović, T.. (2023). Characterization of Pseudomonas syringae pv. morsprunorum originating from sweet cherry and plum in Serbia. in Genetika
Serbian Genetics Society., 55(1), 159-176.
https://doi.org/10.2298/gensr2301159i

Iličić R, Jelušić A, Blagojević M, Bagi F, Vrandečić K, Stanković S, Popović Milovanović T. Characterization of Pseudomonas syringae pv. morsprunorum originating from sweet cherry and plum in Serbia. in Genetika. 2023;55(1):159-176.
doi:10.2298/gensr2301159i .

Iličić, Renata, Jelušić, Aleksandra, Blagojević, Milan, Bagi, Ferenc, Vrandečić, Karolina, Stanković, Slaviša, Popović Milovanović, Tatjana, "Characterization of Pseudomonas syringae pv. morsprunorum originating from sweet cherry and plum in Serbia" in Genetika, 55, no. 1 (2023):159-176,
https://doi.org/10.2298/gensr2301159i . .

TY  - JOUR
AU  - Trkulja, Vojislav
AU  - Tomić, Andrija
AU  - Iličić, Renata
AU  - Nožinić, Miloš
AU  - Popović Milovanović, Tatjana
PY  - 2022
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/855
AB  - Xylella fastidiosa is xylem-limited bacterium capable of infecting a wide range of host plants, resulting in Pierce’s disease in grapevine, citrus variegated chlorosis, olive quick decline syndrome, peach phony disease, plum leaf scald, alfalfa dwarf, margin necrosis and leaf scorch affecting oleander, coffee, almond, pecan, mulberry, red maple, oak, and other types of cultivated and ornamental plants and forest trees. In the European Union, X. fastidiosa is listed as a quarantine organism. Since its first outbreak in the Apulia region of southern Italy in 2013 where it caused devastating disease on Olea europaea (called olive leaf scorch and quick decline), X. fastidiosa continued to spread and successfully established in some European countries (Corsica and PACA in France, Balearic Islands, Madrid and Comunitat Valenciana in Spain, and Porto in Portugal). The most recent data for Europe indicates that X. fastidiosa is present on 174 hosts, 25 of which were newly identified in 2021 (with further five hosts discovered in other parts of the world in the same year). From the six reported subspecies of X. fastidiosa worldwide, four have been recorded in European countries (fastidiosa, multiplex, pauca, and sandyi). Currently confirmed X. fastidiosa vector species are Philaenus spumarius, Neophilaenus campestris, and Philaenus italosignus, whereby only P. spumarius (which has been identified as the key vector in Apulia, Italy) is also present in Americas. X. fastidiosa control is currently based on pathogen-free propagation plant material, eradication, territory demarcation, and vector control, as well as use of resistant plant cultivars and bactericidal treatments.
PB  - The Korean Society of Plant Pathology
T2  - The Plant Pathology Journal
T1  - Xylella fastidiosa in Europe: From the Introduction to the Current Status
EP  - 571
IS  - 6
SP  - 551
VL  - 38
DO  - 10.5423/PPJ.RW.09.2022.0127
ER  - 

@article{
author = "Trkulja, Vojislav and Tomić, Andrija and Iličić, Renata and Nožinić, Miloš and Popović Milovanović, Tatjana",
year = "2022",
abstract = "Xylella fastidiosa is xylem-limited bacterium capable of infecting a wide range of host plants, resulting in Pierce’s disease in grapevine, citrus variegated chlorosis, olive quick decline syndrome, peach phony disease, plum leaf scald, alfalfa dwarf, margin necrosis and leaf scorch affecting oleander, coffee, almond, pecan, mulberry, red maple, oak, and other types of cultivated and ornamental plants and forest trees. In the European Union, X. fastidiosa is listed as a quarantine organism. Since its first outbreak in the Apulia region of southern Italy in 2013 where it caused devastating disease on Olea europaea (called olive leaf scorch and quick decline), X. fastidiosa continued to spread and successfully established in some European countries (Corsica and PACA in France, Balearic Islands, Madrid and Comunitat Valenciana in Spain, and Porto in Portugal). The most recent data for Europe indicates that X. fastidiosa is present on 174 hosts, 25 of which were newly identified in 2021 (with further five hosts discovered in other parts of the world in the same year). From the six reported subspecies of X. fastidiosa worldwide, four have been recorded in European countries (fastidiosa, multiplex, pauca, and sandyi). Currently confirmed X. fastidiosa vector species are Philaenus spumarius, Neophilaenus campestris, and Philaenus italosignus, whereby only P. spumarius (which has been identified as the key vector in Apulia, Italy) is also present in Americas. X. fastidiosa control is currently based on pathogen-free propagation plant material, eradication, territory demarcation, and vector control, as well as use of resistant plant cultivars and bactericidal treatments.",
publisher = "The Korean Society of Plant Pathology",
journal = "The Plant Pathology Journal",
title = "Xylella fastidiosa in Europe: From the Introduction to the Current Status",
pages = "571-551",
number = "6",
volume = "38",
doi = "10.5423/PPJ.RW.09.2022.0127"
}

Trkulja, V., Tomić, A., Iličić, R., Nožinić, M.,& Popović Milovanović, T.. (2022). Xylella fastidiosa in Europe: From the Introduction to the Current Status. in The Plant Pathology Journal
The Korean Society of Plant Pathology., 38(6), 551-571.
https://doi.org/10.5423/PPJ.RW.09.2022.0127

Trkulja V, Tomić A, Iličić R, Nožinić M, Popović Milovanović T. Xylella fastidiosa in Europe: From the Introduction to the Current Status. in The Plant Pathology Journal. 2022;38(6):551-571.
doi:10.5423/PPJ.RW.09.2022.0127 .

Trkulja, Vojislav, Tomić, Andrija, Iličić, Renata, Nožinić, Miloš, Popović Milovanović, Tatjana, "Xylella fastidiosa in Europe: From the Introduction to the Current Status" in The Plant Pathology Journal, 38, no. 6 (2022):551-571,
https://doi.org/10.5423/PPJ.RW.09.2022.0127 . .

TY  - CONF
AU  - Marković, Sanja
AU  - Popović Milovanović, Tatjana
AU  - Jelušić, Aleksandra
AU  - Iličić, Renata
AU  - Stanković, Slaviša
PY  - 2020
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/784
AB  - Background: Bacterial pathogens Ralstonia solanacearum, Clavibacter michiganensis subsp. sepedonicus and Dickeya dianthicola are quarantine bacteria in EPPO region and lead to severity losses in potato production. Widespread Pectobacterium carotovorum subsp. brasiliensis also causes damages under favorable conditions. The available bactericides are not enough effective, therefore biological agents in their control are emerging as a possible solution. Objectives: The aim of this study was evaluation of antibacterial activity of Bacillus amyloliquefaciens against recently found bacteria in Serbian potatoes (R. solanacearum, D. dianthicola and P. c. subsp. brasiliensis). Methods: Two antagonistic B. amyloliquefaciens strains coded as SS-12.6 and SS-38.4 were used in in vitro screening of antimicrobial activity against R. solanacearum (strain Rs81/18), D. dianthicola (strain Dd31) and P. c. subsp. brasiliensis (strain Pcb62). Pathogens and antagonists strains were grown in LB medium for 48 h at 26 C° and 30 °C, respectively. Supernatants and suspended pellet of full culture of SS-12.6 and SS-38.4 were tested by well diffusion assay and dropplate method, respectively. Diameter of inhibition zones were measured and expressed in mm. Biofilm formation was performed on microtitre plates and measured by multi-well plate readers. Results: Supernatants of antagonists formed inhibition zones for R. solanacearum (SS-12.6 10×10 mm; SS-38.4 11×11 mm) and P. c. subsp. brasiliensis (SS-12.6 10×10 mm; 38.4 12×12 mm), while result for D. dianthicola was negative. Pellet of full antagonist cultures produced inhibition zones only for R. solanacearum (SS-12.6 10×11 mm; 38.4 11×11 mm). The biofilm were formed in poor formation for all tested pathogens (category 1).
C3  - FEMS Online Conference on Microbiology 2020 28 - 31 October
T1  - Potential of Bacillus amyloliquefaciens strains SS-12.6 and SS-38.4 in biological control of potato rot pathogens
SP  - 178
ER  - 

@conference{
author = "Marković, Sanja and Popović Milovanović, Tatjana and Jelušić, Aleksandra and Iličić, Renata and Stanković, Slaviša",
year = "2020",
abstract = "Background: Bacterial pathogens Ralstonia solanacearum, Clavibacter michiganensis subsp. sepedonicus and Dickeya dianthicola are quarantine bacteria in EPPO region and lead to severity losses in potato production. Widespread Pectobacterium carotovorum subsp. brasiliensis also causes damages under favorable conditions. The available bactericides are not enough effective, therefore biological agents in their control are emerging as a possible solution. Objectives: The aim of this study was evaluation of antibacterial activity of Bacillus amyloliquefaciens against recently found bacteria in Serbian potatoes (R. solanacearum, D. dianthicola and P. c. subsp. brasiliensis). Methods: Two antagonistic B. amyloliquefaciens strains coded as SS-12.6 and SS-38.4 were used in in vitro screening of antimicrobial activity against R. solanacearum (strain Rs81/18), D. dianthicola (strain Dd31) and P. c. subsp. brasiliensis (strain Pcb62). Pathogens and antagonists strains were grown in LB medium for 48 h at 26 C° and 30 °C, respectively. Supernatants and suspended pellet of full culture of SS-12.6 and SS-38.4 were tested by well diffusion assay and dropplate method, respectively. Diameter of inhibition zones were measured and expressed in mm. Biofilm formation was performed on microtitre plates and measured by multi-well plate readers. Results: Supernatants of antagonists formed inhibition zones for R. solanacearum (SS-12.6 10×10 mm; SS-38.4 11×11 mm) and P. c. subsp. brasiliensis (SS-12.6 10×10 mm; 38.4 12×12 mm), while result for D. dianthicola was negative. Pellet of full antagonist cultures produced inhibition zones only for R. solanacearum (SS-12.6 10×11 mm; 38.4 11×11 mm). The biofilm were formed in poor formation for all tested pathogens (category 1).",
journal = "FEMS Online Conference on Microbiology 2020 28 - 31 October",
title = "Potential of Bacillus amyloliquefaciens strains SS-12.6 and SS-38.4 in biological control of potato rot pathogens",
pages = "178"
}

Marković, S., Popović Milovanović, T., Jelušić, A., Iličić, R.,& Stanković, S.. (2020). Potential of Bacillus amyloliquefaciens strains SS-12.6 and SS-38.4 in biological control of potato rot pathogens. in FEMS Online Conference on Microbiology 2020 28 - 31 October, 178.

Marković S, Popović Milovanović T, Jelušić A, Iličić R, Stanković S. Potential of Bacillus amyloliquefaciens strains SS-12.6 and SS-38.4 in biological control of potato rot pathogens. in FEMS Online Conference on Microbiology 2020 28 - 31 October. 2020;:178..

Marković, Sanja, Popović Milovanović, Tatjana, Jelušić, Aleksandra, Iličić, Renata, Stanković, Slaviša, "Potential of Bacillus amyloliquefaciens strains SS-12.6 and SS-38.4 in biological control of potato rot pathogens" in FEMS Online Conference on Microbiology 2020 28 - 31 October (2020):178.