TY  - CONF
AU  - Živković, Svetlana
AU  - Trkulja, Nenad
AU  - Kovačević, Stefan
AU  - Stošić, Stefan
PY  - 2023
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/1202
AB  - Kruška (Pyrus communis L.) je jedna od najstarijih i najrasprostranjenijih voćarskih
kultura. Plodovi kruške su izrazito bogati nutrijentima i niskog glikemijskog indeksa, pa se
preporučuju u ishrani svih kategorija stanovništva. U periodu vegetacije i nakon berbe
propadanje plodova kruške mogu izazvati brojni biljni patogeni, među kojima su i gljive roda
Colletotricum.
Tokom jeseni 2021. godine iz skladišta u okolini Smedereva sakupljeni su plodovi
kruške (cv. Santa Maria) sa simptomima antraknoze. Na površini svih inficiranih plodova bile
su prisutne tamne, kružne, udubljene nekrotične lezije sa koncentrično raspoređenim
acervulima i narandžastom konidijalnom masom. Izolacija patogena je obavljena standarnim
fitopatološkim metodama na podlozi od krompir-dekstroznog agara (KDA). Svi dobijeni izolati
su nakon 7 dana na KDA i temperaturi od 25⁰C ispoljili uniformne morfološke osobine.
Formirane kolonije su bile svetlosive do svetloružičaste, sa naličja tamnoružičaste do
vinastocrvene boje. Prosečan porast kolonija je iznosio 64±2 mm. Konidije su bile hijalinske,
fuziformne, neseptirane, glatkih zidova, sa oba zaoštrena kraja, veličine 12,8 - 16,6 × 3,5 – 4,4
µm (n=100); a apresorije pojedinačne, tamnobraon boje, okrugle do nepravilnog oblika,
glatkih zidova, veličine 5,5 – 9,6 × 4,8 – 7,2 µm (n=20). Morfološke karakteristike izolata su
odgovarale literaturnom opisu C. acutatum species complex.
Molekularna identifikacija reprezentativnog izolata SZ-21-36 je obavljena na osnovu
ITS regiona rDNA korišćenjem prajmera ITS1/ITS4; dela gena za beta tubulin (Ben A)
primenom prajmera Bt2a/Bt2b; gliceraldehid 3 - fosfat dehidrogenaze (GAPDH) sa
prajmerima GDF1/GDR1, i dela aktin gena (ACT) upotrebom prajmera ACT512F/ACT783R.
Dobijeni PCR produkti su sekvencirani, a nukleotidne sekvence deponovane u GenBank bazi
podataka. BLAST analizom je utvrđeno da su sekvence ON171625 (ITS) i ON186698 (ACT)
99% slične, a sekvence ON186696 (BenA) i ON186697 (GAPDH) identične sa sekvencama
istih regiona izolata vrste C. fioriniae deponovanih u GenBank bazi. Multilokus filogenetska
analiza zasnovana na navedenim molekularnim markerima je potvrdila rezultate BLAST-a, i
svrstala izolat SZ-21-36 sa ostalim izolatima C. fioriniae iz GenBank baze podataka (100%
bootstrap podrška).
Provera patogenosti izolata obavljena je inokulacijom sa 50 μl suspenzije spora (1 ×
106 konidija/ml) u prethodno napravljene povrede na zdravim plodovima kruške (cv. Santa
Maria). Kontrolni plodovi su inokulisani sterilnom destilovanom vodom. Nakon 10 dana
inkubacije u vlažnoj komori na 25°C na svim inokulisanim plodovima kruške razvili su se
tipični simptomi antraknoze, dok su kontrolni plodovi bili bez simptoma. Kulture dobijene  reizolacijom bile su istih morfoloških karakteristika čime je potvrđena patogenost testiranih
izolata.
U ovom istraživanju je konvencionalnim i molekularnim metodama prvi put u Srbiji
identifikovana vrsta C. fioriniae - prouzrokovač antraknoze plodova kruške.
PB  - Društvo za zaštitu bilja
C3  - XVII simpozijum o zaštiti bilja, Zlatibor 2023, 27-30. novembar, Zbornik rezimea radova
T1  - Colletotrichum fioriniae  prouzrokovač antraknoze plodova kruške
EP  - 45
SP  - 44
ER  - 

@conference{
author = "Živković, Svetlana and Trkulja, Nenad and Kovačević, Stefan and Stošić, Stefan",
year = "2023",
abstract = "Kruška (Pyrus communis L.) je jedna od najstarijih i najrasprostranjenijih voćarskih
kultura. Plodovi kruške su izrazito bogati nutrijentima i niskog glikemijskog indeksa, pa se
preporučuju u ishrani svih kategorija stanovništva. U periodu vegetacije i nakon berbe
propadanje plodova kruške mogu izazvati brojni biljni patogeni, među kojima su i gljive roda
Colletotricum.
Tokom jeseni 2021. godine iz skladišta u okolini Smedereva sakupljeni su plodovi
kruške (cv. Santa Maria) sa simptomima antraknoze. Na površini svih inficiranih plodova bile
su prisutne tamne, kružne, udubljene nekrotične lezije sa koncentrično raspoređenim
acervulima i narandžastom konidijalnom masom. Izolacija patogena je obavljena standarnim
fitopatološkim metodama na podlozi od krompir-dekstroznog agara (KDA). Svi dobijeni izolati
su nakon 7 dana na KDA i temperaturi od 25⁰C ispoljili uniformne morfološke osobine.
Formirane kolonije su bile svetlosive do svetloružičaste, sa naličja tamnoružičaste do
vinastocrvene boje. Prosečan porast kolonija je iznosio 64±2 mm. Konidije su bile hijalinske,
fuziformne, neseptirane, glatkih zidova, sa oba zaoštrena kraja, veličine 12,8 - 16,6 × 3,5 – 4,4
µm (n=100); a apresorije pojedinačne, tamnobraon boje, okrugle do nepravilnog oblika,
glatkih zidova, veličine 5,5 – 9,6 × 4,8 – 7,2 µm (n=20). Morfološke karakteristike izolata su
odgovarale literaturnom opisu C. acutatum species complex.
Molekularna identifikacija reprezentativnog izolata SZ-21-36 je obavljena na osnovu
ITS regiona rDNA korišćenjem prajmera ITS1/ITS4; dela gena za beta tubulin (Ben A)
primenom prajmera Bt2a/Bt2b; gliceraldehid 3 - fosfat dehidrogenaze (GAPDH) sa
prajmerima GDF1/GDR1, i dela aktin gena (ACT) upotrebom prajmera ACT512F/ACT783R.
Dobijeni PCR produkti su sekvencirani, a nukleotidne sekvence deponovane u GenBank bazi
podataka. BLAST analizom je utvrđeno da su sekvence ON171625 (ITS) i ON186698 (ACT)
99% slične, a sekvence ON186696 (BenA) i ON186697 (GAPDH) identične sa sekvencama
istih regiona izolata vrste C. fioriniae deponovanih u GenBank bazi. Multilokus filogenetska
analiza zasnovana na navedenim molekularnim markerima je potvrdila rezultate BLAST-a, i
svrstala izolat SZ-21-36 sa ostalim izolatima C. fioriniae iz GenBank baze podataka (100%
bootstrap podrška).
Provera patogenosti izolata obavljena je inokulacijom sa 50 μl suspenzije spora (1 ×
106 konidija/ml) u prethodno napravljene povrede na zdravim plodovima kruške (cv. Santa
Maria). Kontrolni plodovi su inokulisani sterilnom destilovanom vodom. Nakon 10 dana
inkubacije u vlažnoj komori na 25°C na svim inokulisanim plodovima kruške razvili su se
tipični simptomi antraknoze, dok su kontrolni plodovi bili bez simptoma. Kulture dobijene  reizolacijom bile su istih morfoloških karakteristika čime je potvrđena patogenost testiranih
izolata.
U ovom istraživanju je konvencionalnim i molekularnim metodama prvi put u Srbiji
identifikovana vrsta C. fioriniae - prouzrokovač antraknoze plodova kruške.",
publisher = "Društvo za zaštitu bilja",
journal = "XVII simpozijum o zaštiti bilja, Zlatibor 2023, 27-30. novembar, Zbornik rezimea radova",
title = "Colletotrichum fioriniae  prouzrokovač antraknoze plodova kruške",
pages = "45-44"
}

Živković, S., Trkulja, N., Kovačević, S.,& Stošić, S.. (2023). Colletotrichum fioriniae  prouzrokovač antraknoze plodova kruške. in XVII simpozijum o zaštiti bilja, Zlatibor 2023, 27-30. novembar, Zbornik rezimea radova
Društvo za zaštitu bilja., 44-45.

Živković S, Trkulja N, Kovačević S, Stošić S. Colletotrichum fioriniae  prouzrokovač antraknoze plodova kruške. in XVII simpozijum o zaštiti bilja, Zlatibor 2023, 27-30. novembar, Zbornik rezimea radova. 2023;:44-45..

Živković, Svetlana, Trkulja, Nenad, Kovačević, Stefan, Stošić, Stefan, "Colletotrichum fioriniae  prouzrokovač antraknoze plodova kruške" in XVII simpozijum o zaštiti bilja, Zlatibor 2023, 27-30. novembar, Zbornik rezimea radova (2023):44-45.

TY  - CONF
AU  - Stošić, Stefan
AU  - Zivkovic, Svetlana
PY  - 2023
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/1192
AB  - The genus Talaromyces encompasses a wide range of species that are ubiquitous in nature, thriving in
diverse habitats such as soil, plant debris, and indoor environments. They inhabit different climatic zones
across the globe and play crucial roles in natural ecosystems, through participation in organic matter
decomposition and nutrient cycling. Members of this genus are also important enzyme, pigment and
mycotoxin producers, biocontrol agents, human pathogens and food contaminants (1). Talaromyces spp.
can be plant pathogens and few studies pointed that out (2-5). A polyphasic approach was employed to
identify isolates of Talaromyces originating from diseased stored fruits in our country and subsequently
determine their plant pathogenicity. Following protocols recommended by Yilmaz et al. (1), morphological
examinations were carried out on Czapek yeast autolysate agar (CYA), Malt extract agar (MEA) and
Creatine sucrose agar (CREA). Mycelial growth was also tested at three incubation temperatures (5, 25 and
37°C) while micromorphological features were inspected from MEA-grown cultures. Internal transcribed
spacer (ITS) and beta-tubulin (BenA) regions were used for multilocus sequence typing (1). Molecular
sequences from our isolates were combined with the reference and verified sequences to construct a
combined (ITS+BenA) maximum likelihood phylogenetic tree. A pathogenicity test was conducted on
originating hosts. After combining the results from the identification procedures, two species of
Talaromyces were isolated in this study - Talaromyces minioluteus from pear, quince, tomato and orange
fruits, and T. rugulosus from pear and lemon fruits. Phenotypic characteristics were similar to the previous
descriptions in the literature (1, 6, 7). In pathogenicity assays, all isolates were pathogenic on the originating
hosts. The symptoms in artificial inoculations resembled those found in naturally infected fruits and the
reisolates exhibited the same morphological traits as the original isolates. These are the first detections of
T. minioluteus and T. rugulosus and the first confirmations of their pathogenicity on the beforementioned
plant hosts. The research presented here sheds light on the previously known fungi that can also act as plant
pathogens/food spoilage agents. Their presence on the fruit diminishes its quality and leads to economic
damage. Since different fruit and vegetables are sometimes kept in the same storage facilities, these plant
pathogens residing on them can also contribute to inoculum build-up and cross-contamination phenomena.
PB  - University of Perugia
C3  - XIX Congress of European Mycologists, Perugia, Italy, 4-8 September 2023
T1  - Talaromyces spp. - the postharvest fruit pathogens in Serbia
SP  - 71
ER  - 

@conference{
author = "Stošić, Stefan and Zivkovic, Svetlana",
year = "2023",
abstract = "The genus Talaromyces encompasses a wide range of species that are ubiquitous in nature, thriving in
diverse habitats such as soil, plant debris, and indoor environments. They inhabit different climatic zones
across the globe and play crucial roles in natural ecosystems, through participation in organic matter
decomposition and nutrient cycling. Members of this genus are also important enzyme, pigment and
mycotoxin producers, biocontrol agents, human pathogens and food contaminants (1). Talaromyces spp.
can be plant pathogens and few studies pointed that out (2-5). A polyphasic approach was employed to
identify isolates of Talaromyces originating from diseased stored fruits in our country and subsequently
determine their plant pathogenicity. Following protocols recommended by Yilmaz et al. (1), morphological
examinations were carried out on Czapek yeast autolysate agar (CYA), Malt extract agar (MEA) and
Creatine sucrose agar (CREA). Mycelial growth was also tested at three incubation temperatures (5, 25 and
37°C) while micromorphological features were inspected from MEA-grown cultures. Internal transcribed
spacer (ITS) and beta-tubulin (BenA) regions were used for multilocus sequence typing (1). Molecular
sequences from our isolates were combined with the reference and verified sequences to construct a
combined (ITS+BenA) maximum likelihood phylogenetic tree. A pathogenicity test was conducted on
originating hosts. After combining the results from the identification procedures, two species of
Talaromyces were isolated in this study - Talaromyces minioluteus from pear, quince, tomato and orange
fruits, and T. rugulosus from pear and lemon fruits. Phenotypic characteristics were similar to the previous
descriptions in the literature (1, 6, 7). In pathogenicity assays, all isolates were pathogenic on the originating
hosts. The symptoms in artificial inoculations resembled those found in naturally infected fruits and the
reisolates exhibited the same morphological traits as the original isolates. These are the first detections of
T. minioluteus and T. rugulosus and the first confirmations of their pathogenicity on the beforementioned
plant hosts. The research presented here sheds light on the previously known fungi that can also act as plant
pathogens/food spoilage agents. Their presence on the fruit diminishes its quality and leads to economic
damage. Since different fruit and vegetables are sometimes kept in the same storage facilities, these plant
pathogens residing on them can also contribute to inoculum build-up and cross-contamination phenomena.",
publisher = "University of Perugia",
journal = "XIX Congress of European Mycologists, Perugia, Italy, 4-8 September 2023",
title = "Talaromyces spp. - the postharvest fruit pathogens in Serbia",
pages = "71"
}

Stošić, S.,& Zivkovic, S.. (2023). Talaromyces spp. - the postharvest fruit pathogens in Serbia. in XIX Congress of European Mycologists, Perugia, Italy, 4-8 September 2023
University of Perugia., 71.

Stošić S, Zivkovic S. Talaromyces spp. - the postharvest fruit pathogens in Serbia. in XIX Congress of European Mycologists, Perugia, Italy, 4-8 September 2023. 2023;:71..

Stošić, Stefan, Zivkovic, Svetlana, "Talaromyces spp. - the postharvest fruit pathogens in Serbia" in XIX Congress of European Mycologists, Perugia, Italy, 4-8 September 2023 (2023):71.

Živković, S., Trkulja, N., Kovačević, S.,& Stošić, S.. (2023). First Report of Colletotrichum fioriniae Causing Anthracnose on Pear Fruit in Serbia. in Plant Disease
American Phytopathological Society..
https://doi.org/10.1094/PDIS-05-22-1019-PDN

Živković S, Trkulja N, Kovačević S, Stošić S. First Report of Colletotrichum fioriniae Causing Anthracnose on Pear Fruit in Serbia. in Plant Disease. 2023;.
doi:10.1094/PDIS-05-22-1019-PDN .

Živković, Svetlana, Trkulja, Nenad, Kovačević, Stefan, Stošić, Stefan, "First Report of Colletotrichum fioriniae Causing Anthracnose on Pear Fruit in Serbia" in Plant Disease (2023),
https://doi.org/10.1094/PDIS-05-22-1019-PDN . .

TY  - CONF
AU  - Zivkovic, Svetlana
AU  - Ristić, Lela
AU  - Starović, Mira
AU  - Aleksic, Goran
AU  - Stošić, Stefan
PY  - 2022
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/791
AB  - Tomato (Solanum lycopersicum, L.) is one of the most widely cultivated crops with high content of vitamins and antioxidant lycopene, which are very important for human health. During the growing season and postharvest storage tomato is susceptible to various diseases caused by pathogenic fungi. In July 2019, tomato (cv. Balkan) with symptoms of blue mold decay were collected from market in Belgrade, Serbia. Macroscopic morphology of three obtained monosporic isolates were observed after growth on Czapek yeast autolysate agar (CYA), creatine sucrose agar (CREA), and malt extract agar (MEA) for seven days at 25ºC. Also, selected isolates were incubated at 5, 25, and 37°C for one week on CYA to monitor the effect of different temperature incubation conditions. Colony characteristics and micromorphology of the fungi agreed with the literature descriptions of Penicillium expansum. The conidiophores of isolates were hyaline, mainly terverticillate; stipes usually smooth-walled; metulae and phialides cylindrical; conidia ellipsoidal (3-3,86-4 × 3-3,13-4 μm), greenish, smooth-walled. Total DNA was extracted using DNeasy Plant Mini Kit (Qiagen, Hilden, Germany) and partial β-tubulin (BenA) sequence was amplified with primers Bt2a/Bt2b. BenA sequence of representative isolate ParP/1 was deposited in NCBI GenBank (Accession No. ON186699). Phylogenetic analysis clustered our isolate with other isolates of P. expansum. Pathogenicity test was conducted on symptomless, detached tomato fruits. All tested isolates caused typical blue mold symptoms on tomato fruits after seven days of incubation. To our knowledge, this is the first report of P. expansum causing postharvest fruit decay on tomato in Serbia.
PB  - Univerisity of East Sarajevo, Faculty of Agriculture
C3  - Proceedings of the XIII International Scientific Agricultural Symposium “Agrosym 2022” 6-9 oktobar 2022, 545 - 551
T1  - Penicillium expansum as a postharvest pathogen of tomato fruit in Serbia
ER  - 

@conference{
author = "Zivkovic, Svetlana and Ristić, Lela and Starović, Mira and Aleksic, Goran and Stošić, Stefan",
year = "2022",
abstract = "Tomato (Solanum lycopersicum, L.) is one of the most widely cultivated crops with high content of vitamins and antioxidant lycopene, which are very important for human health. During the growing season and postharvest storage tomato is susceptible to various diseases caused by pathogenic fungi. In July 2019, tomato (cv. Balkan) with symptoms of blue mold decay were collected from market in Belgrade, Serbia. Macroscopic morphology of three obtained monosporic isolates were observed after growth on Czapek yeast autolysate agar (CYA), creatine sucrose agar (CREA), and malt extract agar (MEA) for seven days at 25ºC. Also, selected isolates were incubated at 5, 25, and 37°C for one week on CYA to monitor the effect of different temperature incubation conditions. Colony characteristics and micromorphology of the fungi agreed with the literature descriptions of Penicillium expansum. The conidiophores of isolates were hyaline, mainly terverticillate; stipes usually smooth-walled; metulae and phialides cylindrical; conidia ellipsoidal (3-3,86-4 × 3-3,13-4 μm), greenish, smooth-walled. Total DNA was extracted using DNeasy Plant Mini Kit (Qiagen, Hilden, Germany) and partial β-tubulin (BenA) sequence was amplified with primers Bt2a/Bt2b. BenA sequence of representative isolate ParP/1 was deposited in NCBI GenBank (Accession No. ON186699). Phylogenetic analysis clustered our isolate with other isolates of P. expansum. Pathogenicity test was conducted on symptomless, detached tomato fruits. All tested isolates caused typical blue mold symptoms on tomato fruits after seven days of incubation. To our knowledge, this is the first report of P. expansum causing postharvest fruit decay on tomato in Serbia.",
publisher = "Univerisity of East Sarajevo, Faculty of Agriculture",
journal = "Proceedings of the XIII International Scientific Agricultural Symposium “Agrosym 2022” 6-9 oktobar 2022, 545 - 551",
title = "Penicillium expansum as a postharvest pathogen of tomato fruit in Serbia"
}

Zivkovic, S., Ristić, L., Starović, M., Aleksic, G.,& Stošić, S.. (2022). Penicillium expansum as a postharvest pathogen of tomato fruit in Serbia. in Proceedings of the XIII International Scientific Agricultural Symposium “Agrosym 2022” 6-9 oktobar 2022, 545 - 551
Univerisity of East Sarajevo, Faculty of Agriculture..

Zivkovic S, Ristić L, Starović M, Aleksic G, Stošić S. Penicillium expansum as a postharvest pathogen of tomato fruit in Serbia. in Proceedings of the XIII International Scientific Agricultural Symposium “Agrosym 2022” 6-9 oktobar 2022, 545 - 551. 2022;..

Zivkovic, Svetlana, Ristić, Lela, Starović, Mira, Aleksic, Goran, Stošić, Stefan, "Penicillium expansum as a postharvest pathogen of tomato fruit in Serbia" in Proceedings of the XIII International Scientific Agricultural Symposium “Agrosym 2022” 6-9 oktobar 2022, 545 - 551 (2022).

TY  - CONF
AU  - Starović, Mira
AU  - Ristić, Lela
AU  - Vučurović, Ivan
AU  - Blagojević, Jovana
AU  - Stošić, Stefan
AU  - Živković, Svetlana
AU  - Aleksic, Goran
PY  - 2022
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/787
AB  - Garlic (Allium sativum L.) is the second most cultivated Allium species worldwide. Many viral, fungal and bacterial diseases attack garlic plants and can be a limiting factor to garlic production. Fungal diseases control is mainly conducted by fungicides, which have detrimental impact on the environment. In this work, we explore the possibility of utilizing essential oils (EOs) as antifungal agents against a fungal isolate originated from symptomatic garlic cloves from Kraljevci locality, Srem District, Serbia, morphologically and molecularly identified as Fusarium verticillioides. EOs used in this study originated from several medicinal plants: Turkish pickling herb (Echinophora tenuifolia), oregano (Origanum vulgare), basil (Ocimum basilicum) and myrtle (Myrtus communis). Minimum inhibitory concentrations (MIC) were determined by microdilution method in 96 well microtiter plates. Microtiter plates were incubated for five days at 28°C. The experiment was repeated four times with trifloxystrobin as a positive control. The lowest concentrations without visible growth were defined as the minimal concentrations inhibiting fungal growth. Fungal spores were washed from the surface of potato dextrose agar (PDA) and spore suspension was adjusted to a concentration of approximately 5.0x104 in a final volume of 100μl per well. The values of minimal inhibitory concentration (MIC) were carried out by Duncan’s multiple range tests. An analysis of variance was performed on MIC data for four EOs applied on F. verticillioides. A significance was evaluated at p<0.05. STATISTICA v.7 (StatSoft, Inc.) was used for statistical analyses. The results of the antimicrobial activity of EOs using microdilution method showed a wide range of antifungal activity against F. verticillioides. The basil EO proved to be the most potent one (MIC-0,325±5,10μg/mL), followed by oregano (MIC-0,775±0,05μg/mL), myrtle (MIC-5,5±0,05μg/mL) and Turkish pickling herb (MIC-55±5,10μg/mL). The data obtained here suggest that the selected EOs can be applied as inhibitors to prevent growth of the phytopathogenic fungus F. verticillioides.
PB  - University of East Sarajevo
C3  - Proceedings of the XIII International Scientific Agricultural Symposium “Agrosym October 6-9, 2022
T1  - Antifungal activity of plant essential oils to the Fusarium verticillioides originated from garlic
EP  - 650
IS  - 645
ER  - 

@conference{
author = "Starović, Mira and Ristić, Lela and Vučurović, Ivan and Blagojević, Jovana and Stošić, Stefan and Živković, Svetlana and Aleksic, Goran",
year = "2022",
abstract = "Garlic (Allium sativum L.) is the second most cultivated Allium species worldwide. Many viral, fungal and bacterial diseases attack garlic plants and can be a limiting factor to garlic production. Fungal diseases control is mainly conducted by fungicides, which have detrimental impact on the environment. In this work, we explore the possibility of utilizing essential oils (EOs) as antifungal agents against a fungal isolate originated from symptomatic garlic cloves from Kraljevci locality, Srem District, Serbia, morphologically and molecularly identified as Fusarium verticillioides. EOs used in this study originated from several medicinal plants: Turkish pickling herb (Echinophora tenuifolia), oregano (Origanum vulgare), basil (Ocimum basilicum) and myrtle (Myrtus communis). Minimum inhibitory concentrations (MIC) were determined by microdilution method in 96 well microtiter plates. Microtiter plates were incubated for five days at 28°C. The experiment was repeated four times with trifloxystrobin as a positive control. The lowest concentrations without visible growth were defined as the minimal concentrations inhibiting fungal growth. Fungal spores were washed from the surface of potato dextrose agar (PDA) and spore suspension was adjusted to a concentration of approximately 5.0x104 in a final volume of 100μl per well. The values of minimal inhibitory concentration (MIC) were carried out by Duncan’s multiple range tests. An analysis of variance was performed on MIC data for four EOs applied on F. verticillioides. A significance was evaluated at p<0.05. STATISTICA v.7 (StatSoft, Inc.) was used for statistical analyses. The results of the antimicrobial activity of EOs using microdilution method showed a wide range of antifungal activity against F. verticillioides. The basil EO proved to be the most potent one (MIC-0,325±5,10μg/mL), followed by oregano (MIC-0,775±0,05μg/mL), myrtle (MIC-5,5±0,05μg/mL) and Turkish pickling herb (MIC-55±5,10μg/mL). The data obtained here suggest that the selected EOs can be applied as inhibitors to prevent growth of the phytopathogenic fungus F. verticillioides.",
publisher = "University of East Sarajevo",
journal = "Proceedings of the XIII International Scientific Agricultural Symposium “Agrosym October 6-9, 2022",
title = "Antifungal activity of plant essential oils to the Fusarium verticillioides originated from garlic",
pages = "650",
number = "645"
}

Starović, M., Ristić, L., Vučurović, I., Blagojević, J., Stošić, S., Živković, S.,& Aleksic, G.. (2022). Antifungal activity of plant essential oils to the Fusarium verticillioides originated from garlic. in Proceedings of the XIII International Scientific Agricultural Symposium “Agrosym October 6-9, 2022
University of East Sarajevo.(645).

Starović M, Ristić L, Vučurović I, Blagojević J, Stošić S, Živković S, Aleksic G. Antifungal activity of plant essential oils to the Fusarium verticillioides originated from garlic. in Proceedings of the XIII International Scientific Agricultural Symposium “Agrosym October 6-9, 2022. 2022;(645):null-650..

Starović, Mira, Ristić, Lela, Vučurović, Ivan, Blagojević, Jovana, Stošić, Stefan, Živković, Svetlana, Aleksic, Goran, "Antifungal activity of plant essential oils to the Fusarium verticillioides originated from garlic" in Proceedings of the XIII International Scientific Agricultural Symposium “Agrosym October 6-9, 2022, no. 645 (2022).

TY  - JOUR
AU  - Stošić, Stefan
AU  - Delić, Dušica
AU  - Živković, Svetlana
PY  - 2022
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/627
AB  - Lemon fruits are an important source of vitamin C, potassium, folate, carotenoids, polyphenols, coumarins and terpenes. These lemon compounds have antioxidant and anti-inflammatory properties which have beneficial effects on human health. This re- search aimed to elucidate the etiology of blue and green molds detected on lemon fruits in Serbia. Using integrative identification approach, the obtained isolates were characterized from morphological, physiological, molecular, phylogenetic and pathological aspects. Col- ony growth and morphology were examined on Czapek yeast autolysate agar (CYA), Malt extract agar (MEA) and Creatine sucrose agar (CREA), and on CYA at two additional incu- bation temperatures (5 and 37 °C). For molecular identification, ITS and partial β-tubulin (BenA) genes were sequenced. Phylogenetic relationships were investigated using maximumlikelihood method. A pathogenicity test was carried out and the possible difference in pathogenicity among isolates was assessed with analysis of variance (ANOVA) and subsequent Tukey’s test. Four species were identified: Penicillium expansum, Penicillium digitatum, Penicillium polonicum and Talaromyces rugulosus. All four species proved to be pathogenic on lemon fruits, producing symptoms similar to those observed on naturally infected fruits. The results of this study are the first records of the beforementioned Penicillium/ Talaromyces species as postharvest pathogens on lemon fruits in Serbia and the first world report of T. rugulosus as phytopathogenic on the same host.
PB  - Matica Srpska
T2  - Zbornik Matice srpske za prirodne nauke
T1  - Polyphasic identification of decay agents of lemon fruits in Serbia
SP  - 73
SP  - 87
VL  - 143
DO  - 10.2298/ZMSPN2243073S
ER  - 

@article{
author = "Stošić, Stefan and Delić, Dušica and Živković, Svetlana",
year = "2022",
abstract = "Lemon fruits are an important source of vitamin C, potassium, folate, carotenoids, polyphenols, coumarins and terpenes. These lemon compounds have antioxidant and anti-inflammatory properties which have beneficial effects on human health. This re- search aimed to elucidate the etiology of blue and green molds detected on lemon fruits in Serbia. Using integrative identification approach, the obtained isolates were characterized from morphological, physiological, molecular, phylogenetic and pathological aspects. Col- ony growth and morphology were examined on Czapek yeast autolysate agar (CYA), Malt extract agar (MEA) and Creatine sucrose agar (CREA), and on CYA at two additional incu- bation temperatures (5 and 37 °C). For molecular identification, ITS and partial β-tubulin (BenA) genes were sequenced. Phylogenetic relationships were investigated using maximumlikelihood method. A pathogenicity test was carried out and the possible difference in pathogenicity among isolates was assessed with analysis of variance (ANOVA) and subsequent Tukey’s test. Four species were identified: Penicillium expansum, Penicillium digitatum, Penicillium polonicum and Talaromyces rugulosus. All four species proved to be pathogenic on lemon fruits, producing symptoms similar to those observed on naturally infected fruits. The results of this study are the first records of the beforementioned Penicillium/ Talaromyces species as postharvest pathogens on lemon fruits in Serbia and the first world report of T. rugulosus as phytopathogenic on the same host.",
publisher = "Matica Srpska",
journal = "Zbornik Matice srpske za prirodne nauke",
title = "Polyphasic identification of decay agents of lemon fruits in Serbia",
pages = "73-87",
volume = "143",
doi = "10.2298/ZMSPN2243073S"
}

Stošić, S., Delić, D.,& Živković, S.. (2022). Polyphasic identification of decay agents of lemon fruits in Serbia. in Zbornik Matice srpske za prirodne nauke
Matica Srpska., 143, 73.
https://doi.org/10.2298/ZMSPN2243073S

Stošić S, Delić D, Živković S. Polyphasic identification of decay agents of lemon fruits in Serbia. in Zbornik Matice srpske za prirodne nauke. 2022;143:73.
doi:10.2298/ZMSPN2243073S .

Stošić, Stefan, Delić, Dušica, Živković, Svetlana, "Polyphasic identification of decay agents of lemon fruits in Serbia" in Zbornik Matice srpske za prirodne nauke, 143 (2022):73,
https://doi.org/10.2298/ZMSPN2243073S . .

TY  - CONF
AU  - Zivkovic, Svetlana
AU  - Ristić, Lela
AU  - Starović, Mira
AU  - Aleksic, Goran
AU  - Stošić, Stefan
PY  - 2021
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/817
PB  - Društvo za zaštitu bilja
C3  - XVI simpozijum o zaštiti bilja, Zlatibor 22. do 25. novembra 2021: 66-67
T1  - Antifungalni efekat hitosana prema Talaromyces minioluteus in vitro
ER  - 

@conference{
author = "Zivkovic, Svetlana and Ristić, Lela and Starović, Mira and Aleksic, Goran and Stošić, Stefan",
year = "2021",
publisher = "Društvo za zaštitu bilja",
journal = "XVI simpozijum o zaštiti bilja, Zlatibor 22. do 25. novembra 2021: 66-67",
title = "Antifungalni efekat hitosana prema Talaromyces minioluteus in vitro"
}

Zivkovic, S., Ristić, L., Starović, M., Aleksic, G.,& Stošić, S.. (2021). Antifungalni efekat hitosana prema Talaromyces minioluteus in vitro. in XVI simpozijum o zaštiti bilja, Zlatibor 22. do 25. novembra 2021: 66-67
Društvo za zaštitu bilja..

Zivkovic S, Ristić L, Starović M, Aleksic G, Stošić S. Antifungalni efekat hitosana prema Talaromyces minioluteus in vitro. in XVI simpozijum o zaštiti bilja, Zlatibor 22. do 25. novembra 2021: 66-67. 2021;..

Zivkovic, Svetlana, Ristić, Lela, Starović, Mira, Aleksic, Goran, Stošić, Stefan, "Antifungalni efekat hitosana prema Talaromyces minioluteus in vitro" in XVI simpozijum o zaštiti bilja, Zlatibor 22. do 25. novembra 2021: 66-67 (2021).

TY  - JOUR
AU  - Stošić, Stefan
AU  - Ristić, Lela
AU  - Savković, Željko
AU  - Ljaljevic Grbic, Milica
AU  - Vukojevic, Jelena
AU  - Živković, Svetlana
PY  - 2021
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/726
AB  - Pears are one of the oldest and the third most important fruit species grown in temperate regions. They are consumed because of their nutritional and health benefits, in fresh form or as various processed products. This article resolves the etiology of the Penicillium-like mold symptoms on pear fruits in Serbia. Samples of pear fruits with blue mold and other Penicillium-like mold symptoms were collected in Serbia from 2016 to 2019, from four storages. The recovered isolates were identified and characterized according to a polyphasic approach. Morphological and physiological analyses were performed on three media and five temperatures, respectively. Four loci (internal transcribed spacer, beta-tubulin, calmodulin, and DNA-dependent RNA polymerase II second largest subunit) were used for sequencing, genetic identification, and phylogenetic analyses. The results of the identification by conventional and molecular methods were in agreement, and they revealed that the obtained isolates belong to five species: Penicillium crustosum, P. expansum, P. italicum, Talaromyces minioluteus, and T. rugulosus. In a pathogenicity test, P. crustosum, P. expansum, T. minioluteus, and T. rugulosus produced decay on artificially inoculated pear fruits, and P. italicum induced tissue response lesions. The results of this study are the first reports of T. minioluteus and T. rugulosus as postharvest pear pathogens. Also, these are the first world records of T. minioluteus, T. rugulosus, and P. italicum on fruits of European pear. Furthermore, this is the first finding of P. crustosum, P. expansum, P. italicum, T. minioluteus, and T. rugulosus on pear fruit in Serbia.
PB  - American Phytopathological Society
T2  - Plant Disease
T1  - Penicillium and Talaromyces Species as Postharvest Pathogens of Pear Fruit (Pyrus communis) in Serbia
EP  - 3521
IS  - 11
SP  - 3510
VL  - 105
DO  - 10.1094/PDIS-01-21-0037-RE
ER  - 

@article{
author = "Stošić, Stefan and Ristić, Lela and Savković, Željko and Ljaljevic Grbic, Milica and Vukojevic, Jelena and Živković, Svetlana",
year = "2021",
abstract = "Pears are one of the oldest and the third most important fruit species grown in temperate regions. They are consumed because of their nutritional and health benefits, in fresh form or as various processed products. This article resolves the etiology of the Penicillium-like mold symptoms on pear fruits in Serbia. Samples of pear fruits with blue mold and other Penicillium-like mold symptoms were collected in Serbia from 2016 to 2019, from four storages. The recovered isolates were identified and characterized according to a polyphasic approach. Morphological and physiological analyses were performed on three media and five temperatures, respectively. Four loci (internal transcribed spacer, beta-tubulin, calmodulin, and DNA-dependent RNA polymerase II second largest subunit) were used for sequencing, genetic identification, and phylogenetic analyses. The results of the identification by conventional and molecular methods were in agreement, and they revealed that the obtained isolates belong to five species: Penicillium crustosum, P. expansum, P. italicum, Talaromyces minioluteus, and T. rugulosus. In a pathogenicity test, P. crustosum, P. expansum, T. minioluteus, and T. rugulosus produced decay on artificially inoculated pear fruits, and P. italicum induced tissue response lesions. The results of this study are the first reports of T. minioluteus and T. rugulosus as postharvest pear pathogens. Also, these are the first world records of T. minioluteus, T. rugulosus, and P. italicum on fruits of European pear. Furthermore, this is the first finding of P. crustosum, P. expansum, P. italicum, T. minioluteus, and T. rugulosus on pear fruit in Serbia.",
publisher = "American Phytopathological Society",
journal = "Plant Disease",
title = "Penicillium and Talaromyces Species as Postharvest Pathogens of Pear Fruit (Pyrus communis) in Serbia",
pages = "3521-3510",
number = "11",
volume = "105",
doi = "10.1094/PDIS-01-21-0037-RE"
}

Stošić, S., Ristić, L., Savković, Ž., Ljaljevic Grbic, M., Vukojevic, J.,& Živković, S.. (2021). Penicillium and Talaromyces Species as Postharvest Pathogens of Pear Fruit (Pyrus communis) in Serbia. in Plant Disease
American Phytopathological Society., 105(11), 3510-3521.
https://doi.org/10.1094/PDIS-01-21-0037-RE

Stošić S, Ristić L, Savković Ž, Ljaljevic Grbic M, Vukojevic J, Živković S. Penicillium and Talaromyces Species as Postharvest Pathogens of Pear Fruit (Pyrus communis) in Serbia. in Plant Disease. 2021;105(11):3510-3521.
doi:10.1094/PDIS-01-21-0037-RE .

Stošić, Stefan, Ristić, Lela, Savković, Željko, Ljaljevic Grbic, Milica, Vukojevic, Jelena, Živković, Svetlana, "Penicillium and Talaromyces Species as Postharvest Pathogens of Pear Fruit (Pyrus communis) in Serbia" in Plant Disease, 105, no. 11 (2021):3510-3521,
https://doi.org/10.1094/PDIS-01-21-0037-RE . .

Zivkovic, S., Ristić, D.,& Stošić, S.. (2021). First report of Penicillium olsonii causing postharvest fruit rot on tomato in Serbia. in Plant Disease
American Phytopathological Society., 105(8), 2246-2246.
https://doi.org/10.1094/PDIS-02-21-0323-PDN

Zivkovic S, Ristić D, Stošić S. First report of Penicillium olsonii causing postharvest fruit rot on tomato in Serbia. in Plant Disease. 2021;105(8):2246-2246.
doi:10.1094/PDIS-02-21-0323-PDN .

Zivkovic, Svetlana, Ristić, Danijela, Stošić, Stefan, "First report of Penicillium olsonii causing postharvest fruit rot on tomato in Serbia" in Plant Disease, 105, no. 8 (2021):2246-2246,
https://doi.org/10.1094/PDIS-02-21-0323-PDN . .

TY  - JOUR
AU  - Stošić, Stefan
AU  - Ristić, Danijela
AU  - Živković, Svetlana
PY  - 2021
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/620
AB  - Mandarin fruits are one of the most popular among the Citrus genus. They are consumed because of their nutritional and health benefits, as well as pleasant taste and smell. This paper describes the identification and characterization of Penicillium expansum, isolated from molded mandarin fruits. The obtained isolates were cultivated on five media [Czapek Yeast Autolysate agar (CYA), MEA (Malt extract agar), Creatine sucrose agar (CREA), Yeast extract sucrose agar (YES), and Oatmeal agar (OA)] and at five different incubation temperatures (5, 15, 25, 30, and 37 °C). Isolates were sequenced for two molecular loci: internal transcribed spacer and beta-tubulin. Based on the results from morphological, physiological, molecular, and phylogenetic analyses, the recovered isolates were identified as P. expansum. The isolated species was confirmed as pathogenic to mandarin fruits in a pathogenicity test. To the best of our knowledge, this is the first report of P. expansum as a postharvest pathogen of mandarin fruit in Serbia.
PB  - Matica Srpska
T2  - Zbornik Matice srpske za prirodne nauke
T1  - Postharvest decay of mandarin fruit in Serbia caused by Penicillium expansum
EP  - 44
SP  - 29
VL  - 140
DO  - 10.2298/ZMSPN2140029S
ER  - 

@article{
author = "Stošić, Stefan and Ristić, Danijela and Živković, Svetlana",
year = "2021",
abstract = "Mandarin fruits are one of the most popular among the Citrus genus. They are consumed because of their nutritional and health benefits, as well as pleasant taste and smell. This paper describes the identification and characterization of Penicillium expansum, isolated from molded mandarin fruits. The obtained isolates were cultivated on five media [Czapek Yeast Autolysate agar (CYA), MEA (Malt extract agar), Creatine sucrose agar (CREA), Yeast extract sucrose agar (YES), and Oatmeal agar (OA)] and at five different incubation temperatures (5, 15, 25, 30, and 37 °C). Isolates were sequenced for two molecular loci: internal transcribed spacer and beta-tubulin. Based on the results from morphological, physiological, molecular, and phylogenetic analyses, the recovered isolates were identified as P. expansum. The isolated species was confirmed as pathogenic to mandarin fruits in a pathogenicity test. To the best of our knowledge, this is the first report of P. expansum as a postharvest pathogen of mandarin fruit in Serbia.",
publisher = "Matica Srpska",
journal = "Zbornik Matice srpske za prirodne nauke",
title = "Postharvest decay of mandarin fruit in Serbia caused by Penicillium expansum",
pages = "44-29",
volume = "140",
doi = "10.2298/ZMSPN2140029S"
}

Stošić, S., Ristić, D.,& Živković, S.. (2021). Postharvest decay of mandarin fruit in Serbia caused by Penicillium expansum. in Zbornik Matice srpske za prirodne nauke
Matica Srpska., 140, 29-44.
https://doi.org/10.2298/ZMSPN2140029S

Stošić S, Ristić D, Živković S. Postharvest decay of mandarin fruit in Serbia caused by Penicillium expansum. in Zbornik Matice srpske za prirodne nauke. 2021;140:29-44.
doi:10.2298/ZMSPN2140029S .

Stošić, Stefan, Ristić, Danijela, Živković, Svetlana, "Postharvest decay of mandarin fruit in Serbia caused by Penicillium expansum" in Zbornik Matice srpske za prirodne nauke, 140 (2021):29-44,
https://doi.org/10.2298/ZMSPN2140029S . .

TY  - JOUR
AU  - Stošić, Stefan
AU  - Ristić, Lela
AU  - Gašić, Katarina
AU  - Starović, Mira
AU  - Ljaljevic Grbic, Milica
AU  - Vukojevic, Jelena
AU  - Živković, Svetlana
PY  - 2020
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/717
AB  - Talaromyces minioluteus is one of the important species of genus Talaromyces, which has cosmopolitan distribution and is encountered on a wide range of different habitats. This species has not been considered as an important plant pathogen, even though it has been isolated from various plant hosts. Fruits and vegetables with Penicillium-like mold symptoms were collected from 2015 to 2017 from markets in Serbia. Isolates originating from quince, tomato, and orange fruits, onion bulbs, and potato tubers were identified and characterized on a morphological, physiological, and molecular level. Morphological and physiological examination included observing micromorphology, testing growth on six different media and at five different temperatures, and production of three enzymes. Molecular identification and characterization were performed using four molecular markers: internal transcribed spacer, β-tubulin, calmodulin, and DNA-dependent RNA polymerase II second largest subunit. The results of morphological and molecular analyses were in agreement, and they proved that the obtained isolates are T. minioluteus. In the pathogenicity assay, T. minioluteus was confirmed as a pathogen of all species tested with the exception of potato tubers. This is the first report of T. minioluteus as a postharvest plant pathogen on quince, tomato, and orange fruit and onion bulbs. Also, this is the first record of T. minioluteus in Serbia.
PB  - The American Phytopathological Society
T2  - Plant Disease
T1  - Talaromyces minioluteus: New Postharvest Fungal Pathogen in Serbia
EP  - 667
IS  - 3
SP  - 656
VL  - 104
DO  - 10.1094/PDIS-08-19-1806-RE
ER  - 

@article{
author = "Stošić, Stefan and Ristić, Lela and Gašić, Katarina and Starović, Mira and Ljaljevic Grbic, Milica and Vukojevic, Jelena and Živković, Svetlana",
year = "2020",
abstract = "Talaromyces minioluteus is one of the important species of genus Talaromyces, which has cosmopolitan distribution and is encountered on a wide range of different habitats. This species has not been considered as an important plant pathogen, even though it has been isolated from various plant hosts. Fruits and vegetables with Penicillium-like mold symptoms were collected from 2015 to 2017 from markets in Serbia. Isolates originating from quince, tomato, and orange fruits, onion bulbs, and potato tubers were identified and characterized on a morphological, physiological, and molecular level. Morphological and physiological examination included observing micromorphology, testing growth on six different media and at five different temperatures, and production of three enzymes. Molecular identification and characterization were performed using four molecular markers: internal transcribed spacer, β-tubulin, calmodulin, and DNA-dependent RNA polymerase II second largest subunit. The results of morphological and molecular analyses were in agreement, and they proved that the obtained isolates are T. minioluteus. In the pathogenicity assay, T. minioluteus was confirmed as a pathogen of all species tested with the exception of potato tubers. This is the first report of T. minioluteus as a postharvest plant pathogen on quince, tomato, and orange fruit and onion bulbs. Also, this is the first record of T. minioluteus in Serbia.",
publisher = "The American Phytopathological Society",
journal = "Plant Disease",
title = "Talaromyces minioluteus: New Postharvest Fungal Pathogen in Serbia",
pages = "667-656",
number = "3",
volume = "104",
doi = "10.1094/PDIS-08-19-1806-RE"
}

Stošić, S., Ristić, L., Gašić, K., Starović, M., Ljaljevic Grbic, M., Vukojevic, J.,& Živković, S.. (2020). Talaromyces minioluteus: New Postharvest Fungal Pathogen in Serbia. in Plant Disease
The American Phytopathological Society., 104(3), 656-667.
https://doi.org/10.1094/PDIS-08-19-1806-RE

Stošić S, Ristić L, Gašić K, Starović M, Ljaljevic Grbic M, Vukojevic J, Živković S. Talaromyces minioluteus: New Postharvest Fungal Pathogen in Serbia. in Plant Disease. 2020;104(3):656-667.
doi:10.1094/PDIS-08-19-1806-RE .

Stošić, Stefan, Ristić, Lela, Gašić, Katarina, Starović, Mira, Ljaljevic Grbic, Milica, Vukojevic, Jelena, Živković, Svetlana, "Talaromyces minioluteus: New Postharvest Fungal Pathogen in Serbia" in Plant Disease, 104, no. 3 (2020):656-667,
https://doi.org/10.1094/PDIS-08-19-1806-RE . .

TY  - JOUR
AU  - Zivkovic, Svetlana
AU  - Stošić, Stefan
AU  - Ristić, Danijela
AU  - Vučurović, Ivan
AU  - Stevanović, Miloš
PY  - 2019
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/917
AB  - Lactobacillus plantarum, one of the most widespread lactic acid bacte¬ria, exert a strong antagonistic activity against many microorganisms. The present study was conducted to determine in vitro and in situ antagonistic potential of L. plantarum (DSM 20174) for control postharvest decay caused by phytopathogenic fungi: Aspergillus flavus, Colletotrichum acutatum, Colletotrichum gloeosporioides, and Fusarium avenaceum. The results obtained in in vitro assays showed that L. plantarum had a stronger inhibitory effect on spore germination than on mycelia growth of all tested fungi. After 3 days of incubation, the diameter of inhibition zones ranged from 11.67 mm for C. gloeosporioides to 14.67 mm for C. acutatum. The bacterial suspension of L. plantarum significantly inhibited conidial germination of all postharvest pathogens (89.62-97.61%). In situ assays showed that treatment with L. plantarum efficiently inhibited necrosis ranging from 42.54% for C. acutatum to 54.47% for A. flavus. The disease incidence in L. plantarum treated fruits was statistically significantly lower than in the positive control for all fungi tested (P<0.05). The presented data demonstrate the antagonistic potential of L. plantarum (DSM 20174) and indicate the possibility of using this bacterial strain as a biological agent to control postharvest fungal pathogens.
PB  - Matica srpska
T2  - Zbornik Matice srpske za prirodne nauke
T1  - Antagonistic potential of lactobacillus plantarum against some postharvest pathogenic fungi
EP  - 88
SP  - 79
VL  - 136
DO  - 10.2298/zmspn1936079z
ER  - 

@article{
author = "Zivkovic, Svetlana and Stošić, Stefan and Ristić, Danijela and Vučurović, Ivan and Stevanović, Miloš",
year = "2019",
abstract = "Lactobacillus plantarum, one of the most widespread lactic acid bacte¬ria, exert a strong antagonistic activity against many microorganisms. The present study was conducted to determine in vitro and in situ antagonistic potential of L. plantarum (DSM 20174) for control postharvest decay caused by phytopathogenic fungi: Aspergillus flavus, Colletotrichum acutatum, Colletotrichum gloeosporioides, and Fusarium avenaceum. The results obtained in in vitro assays showed that L. plantarum had a stronger inhibitory effect on spore germination than on mycelia growth of all tested fungi. After 3 days of incubation, the diameter of inhibition zones ranged from 11.67 mm for C. gloeosporioides to 14.67 mm for C. acutatum. The bacterial suspension of L. plantarum significantly inhibited conidial germination of all postharvest pathogens (89.62-97.61%). In situ assays showed that treatment with L. plantarum efficiently inhibited necrosis ranging from 42.54% for C. acutatum to 54.47% for A. flavus. The disease incidence in L. plantarum treated fruits was statistically significantly lower than in the positive control for all fungi tested (P<0.05). The presented data demonstrate the antagonistic potential of L. plantarum (DSM 20174) and indicate the possibility of using this bacterial strain as a biological agent to control postharvest fungal pathogens.",
publisher = "Matica srpska",
journal = "Zbornik Matice srpske za prirodne nauke",
title = "Antagonistic potential of lactobacillus plantarum against some postharvest pathogenic fungi",
pages = "88-79",
volume = "136",
doi = "10.2298/zmspn1936079z"
}

Zivkovic, S., Stošić, S., Ristić, D., Vučurović, I.,& Stevanović, M.. (2019). Antagonistic potential of lactobacillus plantarum against some postharvest pathogenic fungi. in Zbornik Matice srpske za prirodne nauke
Matica srpska., 136, 79-88.
https://doi.org/10.2298/zmspn1936079z

Zivkovic S, Stošić S, Ristić D, Vučurović I, Stevanović M. Antagonistic potential of lactobacillus plantarum against some postharvest pathogenic fungi. in Zbornik Matice srpske za prirodne nauke. 2019;136:79-88.
doi:10.2298/zmspn1936079z .

Zivkovic, Svetlana, Stošić, Stefan, Ristić, Danijela, Vučurović, Ivan, Stevanović, Miloš, "Antagonistic potential of lactobacillus plantarum against some postharvest pathogenic fungi" in Zbornik Matice srpske za prirodne nauke, 136 (2019):79-88,
https://doi.org/10.2298/zmspn1936079z . .

TY  - JOUR
AU  - Gavrilović, Veljko
AU  - Milićević, Zoran
AU  - Aleksic, Goran
AU  - Živković, Svetlana
AU  - Stošić, Stefan
AU  - Starović, Mira
AU  - Kuzmanović, Slobodan
PY  - 2018
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/1015
PB  - Institut PKB Agroekonomik
T2  - Zbornik Instituta PKB agroekonomik
T1  - Primena bakar citrata pri defolijaciji sadnica višnje i breskve
EP  - 49
IS  - 5
SP  - 41
VL  - 24
ER  - 

@article{
author = "Gavrilović, Veljko and Milićević, Zoran and Aleksic, Goran and Živković, Svetlana and Stošić, Stefan and Starović, Mira and Kuzmanović, Slobodan",
year = "2018",
publisher = "Institut PKB Agroekonomik",
journal = "Zbornik Instituta PKB agroekonomik",
title = "Primena bakar citrata pri defolijaciji sadnica višnje i breskve",
pages = "49-41",
number = "5",
volume = "24"
}

Gavrilović, V., Milićević, Z., Aleksic, G., Živković, S., Stošić, S., Starović, M.,& Kuzmanović, S.. (2018). Primena bakar citrata pri defolijaciji sadnica višnje i breskve. in Zbornik Instituta PKB agroekonomik
Institut PKB Agroekonomik., 24(5), 41-49.

Gavrilović V, Milićević Z, Aleksic G, Živković S, Stošić S, Starović M, Kuzmanović S. Primena bakar citrata pri defolijaciji sadnica višnje i breskve. in Zbornik Instituta PKB agroekonomik. 2018;24(5):41-49..

Gavrilović, Veljko, Milićević, Zoran, Aleksic, Goran, Živković, Svetlana, Stošić, Stefan, Starović, Mira, Kuzmanović, Slobodan, "Primena bakar citrata pri defolijaciji sadnica višnje i breskve" in Zbornik Instituta PKB agroekonomik, 24, no. 5 (2018):41-49.

TY  - JOUR
AU  - Živković, Svetlana
AU  - Stevanović, Miloš
AU  - Đurović, Sanja
AU  - Ristić, Danijela
AU  - Stošić, Stefan
PY  - 2018
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/543
AB  - Chitosan and its derivatives have been reported as a promising alternative for control of postharvest fungal pathogens. The objective of this study was to evaluate in vitro and in situ antifungal activity of chitosan against Alternaria alternata and Colletotrichum gloeosporioides isolated from decayed apple fruits. The fungi were tested in vitro using PDA medium with three concentrations of chitosan (1, 2 and 3 mg/ml). Fungal growth of the test pathogens was significantly affected by all chitosan doses (P lt 0.05) after 7 days of incubation at 25°C. Water solution of 3 mg/ml of chitosan inhibited completely the conidial germination of A. alternata and C. gloeosporioides after 18 h incubation at 25°C. The results obtained from biocontrol assay indicate that the inhibition of postharvest decay of A. alternata and C. gloeosporioides was significantly influenced by chitosan concentrations. Disease incidence in chitosan-treated fruit after 7 days incubation at 25°C was significantly lower than in the positive control for both fungi tested (P lt 0.05). A. alternata and C. gloeosporioides used in this study were progressively inhibited in vitro and in situ with increasing concentrations of chitosan from 1 to 3 mg/ml.
AB  - Istraživanja pokazuju da su hitosan i njegovi derivati dobra alternativa u kontroli skladišnih fitopatogenih gljiva. Cilj ovog rada bio je da ispita antifungalnu aktivnost hitosana prema Alternaria alternata i Colletotrichum gloeosporoides, izolovanih sa inficiranih plodova jabuke, u in vitro i in situ uslovima. Rast gljiva je testiran in vitro koristeći PDA podlogu sa različitim koncentracijama hitosana (1, 2 i 3 mg/ml). Porast oba patogena je bio značajno smanjen (P lt 0.05) u svim koncentracijama hitosana nakon 7 dana inkubacije na 25°C. Vodeni rastvor hitosana koncentracije 3 mg/ml je u potpunosti inhibirao klijanje konidija A. alternata i C. gloeosporoides nakon inkubacije od 18 sati na temperaturi od 25°C. Rezultati ogleda in situ ukazuju da inhibicija propadanja plodova inficiranih sa A. alternata i C. gloeosporoides zavisi od koncentracije rastvora hitosana. Pojava bolesti na plodovima jabuka tretiranih rastvorom hitosana nakon 7 dana inkubacije na 25°C bila je značajno smanjena za obe vrste gljiva (P lt 0.05) u odnosu na pozitivnu kontrolu. Rast A. alternata i C. gloeosporoides je bio progresivno inhibiran in vitro i in situ sa povećanjem koncentracije rastvora hitosana, od 1 do 3 mg/ml.
PB  - Institute of Pesticides and Environmental Protection
T2  - Pesticidi i fitomedicina
T1  - Antifungal activity of chitosan against Alternaria alternata and Colletotrichum gloeosporioides
T1  - Antifungalna aktivnost hitosana prema Alternaria alternata i Colletotrichum gloeosporoides
EP  - 204
IS  - 3-4
SP  - 197
VL  - 33
DO  - 10.2298/PIF1804197Z
ER  - 

@article{
author = "Živković, Svetlana and Stevanović, Miloš and Đurović, Sanja and Ristić, Danijela and Stošić, Stefan",
year = "2018",
abstract = "Chitosan and its derivatives have been reported as a promising alternative for control of postharvest fungal pathogens. The objective of this study was to evaluate in vitro and in situ antifungal activity of chitosan against Alternaria alternata and Colletotrichum gloeosporioides isolated from decayed apple fruits. The fungi were tested in vitro using PDA medium with three concentrations of chitosan (1, 2 and 3 mg/ml). Fungal growth of the test pathogens was significantly affected by all chitosan doses (P lt 0.05) after 7 days of incubation at 25°C. Water solution of 3 mg/ml of chitosan inhibited completely the conidial germination of A. alternata and C. gloeosporioides after 18 h incubation at 25°C. The results obtained from biocontrol assay indicate that the inhibition of postharvest decay of A. alternata and C. gloeosporioides was significantly influenced by chitosan concentrations. Disease incidence in chitosan-treated fruit after 7 days incubation at 25°C was significantly lower than in the positive control for both fungi tested (P lt 0.05). A. alternata and C. gloeosporioides used in this study were progressively inhibited in vitro and in situ with increasing concentrations of chitosan from 1 to 3 mg/ml., Istraživanja pokazuju da su hitosan i njegovi derivati dobra alternativa u kontroli skladišnih fitopatogenih gljiva. Cilj ovog rada bio je da ispita antifungalnu aktivnost hitosana prema Alternaria alternata i Colletotrichum gloeosporoides, izolovanih sa inficiranih plodova jabuke, u in vitro i in situ uslovima. Rast gljiva je testiran in vitro koristeći PDA podlogu sa različitim koncentracijama hitosana (1, 2 i 3 mg/ml). Porast oba patogena je bio značajno smanjen (P lt 0.05) u svim koncentracijama hitosana nakon 7 dana inkubacije na 25°C. Vodeni rastvor hitosana koncentracije 3 mg/ml je u potpunosti inhibirao klijanje konidija A. alternata i C. gloeosporoides nakon inkubacije od 18 sati na temperaturi od 25°C. Rezultati ogleda in situ ukazuju da inhibicija propadanja plodova inficiranih sa A. alternata i C. gloeosporoides zavisi od koncentracije rastvora hitosana. Pojava bolesti na plodovima jabuka tretiranih rastvorom hitosana nakon 7 dana inkubacije na 25°C bila je značajno smanjena za obe vrste gljiva (P lt 0.05) u odnosu na pozitivnu kontrolu. Rast A. alternata i C. gloeosporoides je bio progresivno inhibiran in vitro i in situ sa povećanjem koncentracije rastvora hitosana, od 1 do 3 mg/ml.",
publisher = "Institute of Pesticides and Environmental Protection",
journal = "Pesticidi i fitomedicina",
title = "Antifungal activity of chitosan against Alternaria alternata and Colletotrichum gloeosporioides, Antifungalna aktivnost hitosana prema Alternaria alternata i Colletotrichum gloeosporoides",
pages = "204-197",
number = "3-4",
volume = "33",
doi = "10.2298/PIF1804197Z"
}

Živković, S., Stevanović, M., Đurović, S., Ristić, D.,& Stošić, S.. (2018). Antifungal activity of chitosan against Alternaria alternata and Colletotrichum gloeosporioides. in Pesticidi i fitomedicina
Institute of Pesticides and Environmental Protection., 33(3-4), 197-204.
https://doi.org/10.2298/PIF1804197Z

Živković S, Stevanović M, Đurović S, Ristić D, Stošić S. Antifungal activity of chitosan against Alternaria alternata and Colletotrichum gloeosporioides. in Pesticidi i fitomedicina. 2018;33(3-4):197-204.
doi:10.2298/PIF1804197Z .

Živković, Svetlana, Stevanović, Miloš, Đurović, Sanja, Ristić, Danijela, Stošić, Stefan, "Antifungal activity of chitosan against Alternaria alternata and Colletotrichum gloeosporioides" in Pesticidi i fitomedicina, 33, no. 3-4 (2018):197-204,
https://doi.org/10.2298/PIF1804197Z . .

TY  - JOUR
AU  - Zivkovic, Svetlana
AU  - Stošić, Stefan
AU  - Stevanović, Miloš
AU  - Gašić, Katarina
AU  - Aleksic, Goran
AU  - Vučurović, Ivan
AU  - Ristić, Danijela
PY  - 2017
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/1013
AB  - Anthracnose caused by the fungus Colletotrichum orbiculare is one of the most significant diseases of Cucurbitaceae. In Serbia watermelon fruits with typical anthracnose lesions were collected during the year of 2015. Affected fruits showed sunken, dark brown to black lesions with orange conidial masses produced in black acervuli. In an attempt to identify the causal organism, small pieces of necrotic tissue were surface sterilized and placed on potato dextrose agar (PDA). Macroscopic and microscopic morphologicaly characteristics of three isolates were observed after growth on PDA for 7 days at 25 ºC under a 12 h light/dark cycle. Fungal colonies developed white, grey to black dense aerial mycelium. Conidia were hyaline, aseptate, straight and cylindrical to clavate, 9–12.5 μm × 4–5.5 μm. Fungal isolates were also characterized by sequencing of the internal transcribed spacer (ITS) rDNA region using ITS1F/ITS4 primers and β-tubuline 2 gene using T1/Bt2b primers. The nucleotide sequences were deposited in GenBank (ITS Acc. No. KT454386, KT454387 and KT454388; β-tubuline 2 gene Acc. No. KT581236, KT581237 and KT581238). BLAST analysis of ITS and β-tubuline 2 gene sequences showed that our isolates were 100% identical to other C. orbiculare in NCBI GenBank. Pathogenicity test was conducted on symptomless, detached watermelon fruits. All tested isolates caused anthracnose lesions on watermelon fruits after 10 days of incubation. Trichoderma harzianum (DSM 63059) and Gliocladium roseum (DSM 62726) were evaluated in vitro for their antagonistic potential against C. orbiculare. The results of this study identify T. harzianum and G. roseum as promising biological control agents (BCAs) for further testing against anthracnose disease on watermelon fruits.
PB  - Herbološko društvo Srbije
T2  - Zbornik Matice srpske za prirodne nauke
T1  - Colletotrichum orbiculare on watermelon - identification and in vitro inhibition by antagonistic fungi
EP  - 343
SP  - 331
VL  - 133
DO  - 10.2298/ZMSPN1733331Z
ER  - 

@article{
author = "Zivkovic, Svetlana and Stošić, Stefan and Stevanović, Miloš and Gašić, Katarina and Aleksic, Goran and Vučurović, Ivan and Ristić, Danijela",
year = "2017",
abstract = "Anthracnose caused by the fungus Colletotrichum orbiculare is one of the most significant diseases of Cucurbitaceae. In Serbia watermelon fruits with typical anthracnose lesions were collected during the year of 2015. Affected fruits showed sunken, dark brown to black lesions with orange conidial masses produced in black acervuli. In an attempt to identify the causal organism, small pieces of necrotic tissue were surface sterilized and placed on potato dextrose agar (PDA). Macroscopic and microscopic morphologicaly characteristics of three isolates were observed after growth on PDA for 7 days at 25 ºC under a 12 h light/dark cycle. Fungal colonies developed white, grey to black dense aerial mycelium. Conidia were hyaline, aseptate, straight and cylindrical to clavate, 9–12.5 μm × 4–5.5 μm. Fungal isolates were also characterized by sequencing of the internal transcribed spacer (ITS) rDNA region using ITS1F/ITS4 primers and β-tubuline 2 gene using T1/Bt2b primers. The nucleotide sequences were deposited in GenBank (ITS Acc. No. KT454386, KT454387 and KT454388; β-tubuline 2 gene Acc. No. KT581236, KT581237 and KT581238). BLAST analysis of ITS and β-tubuline 2 gene sequences showed that our isolates were 100% identical to other C. orbiculare in NCBI GenBank. Pathogenicity test was conducted on symptomless, detached watermelon fruits. All tested isolates caused anthracnose lesions on watermelon fruits after 10 days of incubation. Trichoderma harzianum (DSM 63059) and Gliocladium roseum (DSM 62726) were evaluated in vitro for their antagonistic potential against C. orbiculare. The results of this study identify T. harzianum and G. roseum as promising biological control agents (BCAs) for further testing against anthracnose disease on watermelon fruits.",
publisher = "Herbološko društvo Srbije",
journal = "Zbornik Matice srpske za prirodne nauke",
title = "Colletotrichum orbiculare on watermelon - identification and in vitro inhibition by antagonistic fungi",
pages = "343-331",
volume = "133",
doi = "10.2298/ZMSPN1733331Z"
}

Zivkovic, S., Stošić, S., Stevanović, M., Gašić, K., Aleksic, G., Vučurović, I.,& Ristić, D.. (2017). Colletotrichum orbiculare on watermelon - identification and in vitro inhibition by antagonistic fungi. in Zbornik Matice srpske za prirodne nauke
Herbološko društvo Srbije., 133, 331-343.
https://doi.org/10.2298/ZMSPN1733331Z

Zivkovic S, Stošić S, Stevanović M, Gašić K, Aleksic G, Vučurović I, Ristić D. Colletotrichum orbiculare on watermelon - identification and in vitro inhibition by antagonistic fungi. in Zbornik Matice srpske za prirodne nauke. 2017;133:331-343.
doi:10.2298/ZMSPN1733331Z .

Zivkovic, Svetlana, Stošić, Stefan, Stevanović, Miloš, Gašić, Katarina, Aleksic, Goran, Vučurović, Ivan, Ristić, Danijela, "Colletotrichum orbiculare on watermelon - identification and in vitro inhibition by antagonistic fungi" in Zbornik Matice srpske za prirodne nauke, 133 (2017):331-343,
https://doi.org/10.2298/ZMSPN1733331Z . .

TY  - JOUR
AU  - Gavrilović, Veljko
AU  - Milićević, Zoran
AU  - Aleksić, Goran
AU  - Starović, Mira
AU  - Živković, Svetlana
AU  - Stošić, Stefan
AU  - Kuzmanović, Slobodan
PY  - 2017
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/495
AB  - The effects of copper citrate on defoliation of one year old fruit stocks in a nursery are presented in this paper. The investigation aimed to evaluate the defoliation efficacy of copper citrate as a new formulation, compared with other compounds recommended for that purpose. Field trials were conducted in the region of Trstenik, a center of nursery production in Serbia in 2016 and 2017. Two years of investigation showed that copper citrate could be used as an efficient compound for defoliation in nursery production. A stronger defoliation effect was observed on plum stocks, compared to apple stocks. Better results were achieved with the highest concentration of copper citrate (2%), while the effectiveness decreased with lower concentrations (0.5%, 1.0% and 1.5%). Defoliation degree on nursery apple stocks after treatment with 2.0% concentration of copper citrate varied from 80.6% to 95.6%, while it reached 100% on plum stocks.
AB  - U radu su prikazani efekti defolijacije sadnica jabuke i šljive, primenom različitih koncentarcija bakar citrata, kao nove formulacije, i standardnih preparata koji se koriste u ove svrhe (bordovska čorba, urea). Ogledi su sprovedeni u regionu Trstenika (Ljubava i Milutovac), koji je centar rasadničke proizvodnje u Srbiji. Tretiranja su obavljena tokom 2016. i 2017. godine. Na osnovu dobijenih rezultata zaključeno je da bakar citrat pokazuje visok potencijal za upotrebu u svrhu defolijacije sadnica jabuke i šljive. Korišćene koncentracije ovog jedinjenja su pokazale veću efikasnost u primeni na sadnicama šljive nego na sadnicama jabuke. Najbolji rezultati u oba ogleda su zabeleženi sa maksimalno primenjenom koncentracijom (2%), dok efekat defolijacije na sadnicama jabuke opada sa smanjenjem koncentracije. Stepen defolijacije jabuke primenom bakar citrata u koncentraciji od 2% kretao se od 80,6% do 95,6%, a kod šljive čak 100%. Potvrđeno je i da uspešna defolijacija sadnica jabuke i šljive značajno zavisi od vremena aplikacije kao i meteoroloških uslova.
PB  - Institute of Pesticides and Environmental Protection
T2  - Pesticidi i fitomedicina
T1  - Effects of copper citrate as a defoliant in nursery fruit stock production
T1  - Bakar citrat - defolijant u proizvodnji sadnica voćaka
EP  - 236
IS  - 3-4
SP  - 231
VL  - 32
DO  - 10.2298/PIF1704231G
ER  - 

@article{
author = "Gavrilović, Veljko and Milićević, Zoran and Aleksić, Goran and Starović, Mira and Živković, Svetlana and Stošić, Stefan and Kuzmanović, Slobodan",
year = "2017",
abstract = "The effects of copper citrate on defoliation of one year old fruit stocks in a nursery are presented in this paper. The investigation aimed to evaluate the defoliation efficacy of copper citrate as a new formulation, compared with other compounds recommended for that purpose. Field trials were conducted in the region of Trstenik, a center of nursery production in Serbia in 2016 and 2017. Two years of investigation showed that copper citrate could be used as an efficient compound for defoliation in nursery production. A stronger defoliation effect was observed on plum stocks, compared to apple stocks. Better results were achieved with the highest concentration of copper citrate (2%), while the effectiveness decreased with lower concentrations (0.5%, 1.0% and 1.5%). Defoliation degree on nursery apple stocks after treatment with 2.0% concentration of copper citrate varied from 80.6% to 95.6%, while it reached 100% on plum stocks., U radu su prikazani efekti defolijacije sadnica jabuke i šljive, primenom različitih koncentarcija bakar citrata, kao nove formulacije, i standardnih preparata koji se koriste u ove svrhe (bordovska čorba, urea). Ogledi su sprovedeni u regionu Trstenika (Ljubava i Milutovac), koji je centar rasadničke proizvodnje u Srbiji. Tretiranja su obavljena tokom 2016. i 2017. godine. Na osnovu dobijenih rezultata zaključeno je da bakar citrat pokazuje visok potencijal za upotrebu u svrhu defolijacije sadnica jabuke i šljive. Korišćene koncentracije ovog jedinjenja su pokazale veću efikasnost u primeni na sadnicama šljive nego na sadnicama jabuke. Najbolji rezultati u oba ogleda su zabeleženi sa maksimalno primenjenom koncentracijom (2%), dok efekat defolijacije na sadnicama jabuke opada sa smanjenjem koncentracije. Stepen defolijacije jabuke primenom bakar citrata u koncentraciji od 2% kretao se od 80,6% do 95,6%, a kod šljive čak 100%. Potvrđeno je i da uspešna defolijacija sadnica jabuke i šljive značajno zavisi od vremena aplikacije kao i meteoroloških uslova.",
publisher = "Institute of Pesticides and Environmental Protection",
journal = "Pesticidi i fitomedicina",
title = "Effects of copper citrate as a defoliant in nursery fruit stock production, Bakar citrat - defolijant u proizvodnji sadnica voćaka",
pages = "236-231",
number = "3-4",
volume = "32",
doi = "10.2298/PIF1704231G"
}

Gavrilović, V., Milićević, Z., Aleksić, G., Starović, M., Živković, S., Stošić, S.,& Kuzmanović, S.. (2017). Effects of copper citrate as a defoliant in nursery fruit stock production. in Pesticidi i fitomedicina
Institute of Pesticides and Environmental Protection., 32(3-4), 231-236.
https://doi.org/10.2298/PIF1704231G

Gavrilović V, Milićević Z, Aleksić G, Starović M, Živković S, Stošić S, Kuzmanović S. Effects of copper citrate as a defoliant in nursery fruit stock production. in Pesticidi i fitomedicina. 2017;32(3-4):231-236.
doi:10.2298/PIF1704231G .

Gavrilović, Veljko, Milićević, Zoran, Aleksić, Goran, Starović, Mira, Živković, Svetlana, Stošić, Stefan, Kuzmanović, Slobodan, "Effects of copper citrate as a defoliant in nursery fruit stock production" in Pesticidi i fitomedicina, 32, no. 3-4 (2017):231-236,
https://doi.org/10.2298/PIF1704231G . .

TY  - JOUR
AU  - Stošić, Stefan
AU  - Stojanović, Saša
AU  - Milosavljević, Anja
AU  - Pfaf-Dolovac, Erika
AU  - Živković, Svetlana
PY  - 2014
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/329
AB  - In the recent years, several studies have shown that calcium salts may have potential as environmentally compatible, nontoxic fungicides for controlling postharvest fungal patho­gens. Therefore, the objective of this study was to evaluate and compare the effects of calcium chloride and calcium hydroxide on in vitro mycelial growth, spore germination and germ tube growth of Colletotrichum acutatum, C. gloeosporioides, Alternaria alternata, and Penicillium expansum. The obtained results showed that the fungal isolates grew sim­ilarly or stimulated in the presence of 1 and 1.5% calcium salts compared to the control. After seven days of incubation, reduction of mycelial growth was observed only on PDA supplemented with 2% calcium salts. Calcium chloride and calcium hydroxide at 1.5% and 2.0% concentrations significantly decreased spore germination and germ tube growth of all fungal isolates. The results of this study show that the tested calcium salts can be used as a alternative treatment against postharvest fungal pathogens C. acutatum, C. gloeosporioides, A. alternata and P. expansum.
AB  - Tokom poslednjih nekoliko godina, više studija je pokazalo da u kontroli gljivičnih skladišnih patogena soli kalcijuma mogu imati potencijal kao ekološki kompatibilni, netoksični fungicidi. Stoga je cilj ovog istraživanja bio da se u in vitro uslovima procene i uporede efekti kalcijum hlorida i kalcijum hidroksida na porast micelije, klijavost konidija i rast klicine cevi gljiva C. acutatum, C. gloeosporioides, A. alternata, i P. expansum. Dobijeni rezultati su pokazali da je u prisustvu 1 i 1,5% kalcijumovih soli porast izolata gljiva u tretmanima sličan ili povećan u odnosu na kontrolu. Nakon 7 dana inkubacije, redukcija porasta micelije je utvđena samo na PDA podlozi sa 2% soli kalcijuma. Kalcijum hlorid i kalcijum hidroksid u koncentracijama od 1,5 i 2,0% značajno smanjuju klijavost konidija i rast klicinih cevi svih ispitivanih izolata gljiva. Rezultati ove studije pokazuju da se testirane soli kalcijuma mogu primeniti kao alternativni tretman u kontroli gljivičnih skladišnih patogena, C. acutatum, C. gloeosporioides, A. alternata i P. expansum.
PB  - Institute for Plant Protection and Environment, Belgrade
T2  - Zaštita bilja
T1  - Effect of calcium salts on postharvest fungal pathogens in vitro
T1  - In vitro efekat kalcijumovih soli na gljivične skladišne patogene
EP  - 46
IS  - 1
SP  - 40
VL  - 65
DO  - 10.5937/zasbilj1401040S
ER  - 

@article{
author = "Stošić, Stefan and Stojanović, Saša and Milosavljević, Anja and Pfaf-Dolovac, Erika and Živković, Svetlana",
year = "2014",
abstract = "In the recent years, several studies have shown that calcium salts may have potential as environmentally compatible, nontoxic fungicides for controlling postharvest fungal patho­gens. Therefore, the objective of this study was to evaluate and compare the effects of calcium chloride and calcium hydroxide on in vitro mycelial growth, spore germination and germ tube growth of Colletotrichum acutatum, C. gloeosporioides, Alternaria alternata, and Penicillium expansum. The obtained results showed that the fungal isolates grew sim­ilarly or stimulated in the presence of 1 and 1.5% calcium salts compared to the control. After seven days of incubation, reduction of mycelial growth was observed only on PDA supplemented with 2% calcium salts. Calcium chloride and calcium hydroxide at 1.5% and 2.0% concentrations significantly decreased spore germination and germ tube growth of all fungal isolates. The results of this study show that the tested calcium salts can be used as a alternative treatment against postharvest fungal pathogens C. acutatum, C. gloeosporioides, A. alternata and P. expansum., Tokom poslednjih nekoliko godina, više studija je pokazalo da u kontroli gljivičnih skladišnih patogena soli kalcijuma mogu imati potencijal kao ekološki kompatibilni, netoksični fungicidi. Stoga je cilj ovog istraživanja bio da se u in vitro uslovima procene i uporede efekti kalcijum hlorida i kalcijum hidroksida na porast micelije, klijavost konidija i rast klicine cevi gljiva C. acutatum, C. gloeosporioides, A. alternata, i P. expansum. Dobijeni rezultati su pokazali da je u prisustvu 1 i 1,5% kalcijumovih soli porast izolata gljiva u tretmanima sličan ili povećan u odnosu na kontrolu. Nakon 7 dana inkubacije, redukcija porasta micelije je utvđena samo na PDA podlozi sa 2% soli kalcijuma. Kalcijum hlorid i kalcijum hidroksid u koncentracijama od 1,5 i 2,0% značajno smanjuju klijavost konidija i rast klicinih cevi svih ispitivanih izolata gljiva. Rezultati ove studije pokazuju da se testirane soli kalcijuma mogu primeniti kao alternativni tretman u kontroli gljivičnih skladišnih patogena, C. acutatum, C. gloeosporioides, A. alternata i P. expansum.",
publisher = "Institute for Plant Protection and Environment, Belgrade",
journal = "Zaštita bilja",
title = "Effect of calcium salts on postharvest fungal pathogens in vitro, In vitro efekat kalcijumovih soli na gljivične skladišne patogene",
pages = "46-40",
number = "1",
volume = "65",
doi = "10.5937/zasbilj1401040S"
}

Stošić, S., Stojanović, S., Milosavljević, A., Pfaf-Dolovac, E.,& Živković, S.. (2014). Effect of calcium salts on postharvest fungal pathogens in vitro. in Zaštita bilja
Institute for Plant Protection and Environment, Belgrade., 65(1), 40-46.
https://doi.org/10.5937/zasbilj1401040S

Stošić S, Stojanović S, Milosavljević A, Pfaf-Dolovac E, Živković S. Effect of calcium salts on postharvest fungal pathogens in vitro. in Zaštita bilja. 2014;65(1):40-46.
doi:10.5937/zasbilj1401040S .

Stošić, Stefan, Stojanović, Saša, Milosavljević, Anja, Pfaf-Dolovac, Erika, Živković, Svetlana, "Effect of calcium salts on postharvest fungal pathogens in vitro" in Zaštita bilja, 65, no. 1 (2014):40-46,
https://doi.org/10.5937/zasbilj1401040S . .

TY  - JOUR
AU  - Gavrilović, Veljko
AU  - Stošić, Stefan
AU  - Stevanović, Miloš
PY  - 2014
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/309
AB  - Severe symptoms of necrosis sweet cherry fruits was recorded during 2014. in region of Šabac. About 30 % of fruits (cv. Summit) were affected and necrosis cover 1/3 of fruit surface in average. Gram negative, fluorescent, oxidative bacterial strains were isolated from diseased tissues. Symptoms similar to those, observed in natural infection were recorded on artificial inoculated cherry fruits (cv. Summit and cv. Summbarst). Isolated strains were HR positive, oxidase, pectinase, arginin dehidrolase negative and levan positive (LOPAT +---+). Same characteristics also own check strains Pseudomonas syringae (CFBP 11). According obtained results it was concluded that necrosis of sweet cherry fruits is caused by Pseudomonas syringae. Further characteristic in order proper detection of pathogens, including molecular methods are underway.
AB  - U radu su prikazani rezultati proučavanja sojeva bakterija izolovanih iz nekrotičnih plodova trešnje sorte Samit (područje Šapca). Nekroza zahvata u proseku oko 1/3 ploda koji potpuno gubi tržišnu vrednost. Bolešću biva zahvaćeno oko 30% plodova. Primenom standardnih bakterioloških metoda (izolacijom na hranljivim podlogama), proverom patogenosti i proučavanjem bakterioloških karakteristika, zaključeno je da pomenute simptome prouzrokuje fitopatogena bakterija Pseudomonas syringae, široko rasprostranjen patogen naročito koštičavih voćaka.
PB  - Institute for Plant Protection and Environment, Belgrade
T2  - Zaštita bilja
T1  - Pseudomonas syringae: Causal agent of sweet cherry fruit necrosis
T1  - Pseudomonas syringae - prouzrokovač nekroze plodova trešnje
EP  - 180
IS  - 4
SP  - 176
VL  - 65
DO  - 10.5937/zasbilj1404176G
ER  - 

@article{
author = "Gavrilović, Veljko and Stošić, Stefan and Stevanović, Miloš",
year = "2014",
abstract = "Severe symptoms of necrosis sweet cherry fruits was recorded during 2014. in region of Šabac. About 30 % of fruits (cv. Summit) were affected and necrosis cover 1/3 of fruit surface in average. Gram negative, fluorescent, oxidative bacterial strains were isolated from diseased tissues. Symptoms similar to those, observed in natural infection were recorded on artificial inoculated cherry fruits (cv. Summit and cv. Summbarst). Isolated strains were HR positive, oxidase, pectinase, arginin dehidrolase negative and levan positive (LOPAT +---+). Same characteristics also own check strains Pseudomonas syringae (CFBP 11). According obtained results it was concluded that necrosis of sweet cherry fruits is caused by Pseudomonas syringae. Further characteristic in order proper detection of pathogens, including molecular methods are underway., U radu su prikazani rezultati proučavanja sojeva bakterija izolovanih iz nekrotičnih plodova trešnje sorte Samit (područje Šapca). Nekroza zahvata u proseku oko 1/3 ploda koji potpuno gubi tržišnu vrednost. Bolešću biva zahvaćeno oko 30% plodova. Primenom standardnih bakterioloških metoda (izolacijom na hranljivim podlogama), proverom patogenosti i proučavanjem bakterioloških karakteristika, zaključeno je da pomenute simptome prouzrokuje fitopatogena bakterija Pseudomonas syringae, široko rasprostranjen patogen naročito koštičavih voćaka.",
publisher = "Institute for Plant Protection and Environment, Belgrade",
journal = "Zaštita bilja",
title = "Pseudomonas syringae: Causal agent of sweet cherry fruit necrosis, Pseudomonas syringae - prouzrokovač nekroze plodova trešnje",
pages = "180-176",
number = "4",
volume = "65",
doi = "10.5937/zasbilj1404176G"
}

Gavrilović, V., Stošić, S.,& Stevanović, M.. (2014). Pseudomonas syringae: Causal agent of sweet cherry fruit necrosis. in Zaštita bilja
Institute for Plant Protection and Environment, Belgrade., 65(4), 176-180.
https://doi.org/10.5937/zasbilj1404176G

Gavrilović V, Stošić S, Stevanović M. Pseudomonas syringae: Causal agent of sweet cherry fruit necrosis. in Zaštita bilja. 2014;65(4):176-180.
doi:10.5937/zasbilj1404176G .

Gavrilović, Veljko, Stošić, Stefan, Stevanović, Miloš, "Pseudomonas syringae: Causal agent of sweet cherry fruit necrosis" in Zaštita bilja, 65, no. 4 (2014):176-180,
https://doi.org/10.5937/zasbilj1404176G . .

TY  - JOUR
AU  - Gavrilović, Veljko
AU  - Stanisavljević, Rade
AU  - Stošić, Stefan
AU  - Stevanović, Miloš
AU  - Aleksić, Goran
AU  - Stajić, Milica
AU  - Dolovac, Nenad
PY  - 2014
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/301
AB  - Susceptibility of different pear cultivars to Erwinia amaylovora by artificial inoculated immature pear fruits are shown in this article. According obtained results significant differences among cultivars are confirmed and they could be divided in four groups. Most susceptibly cultivars were Santa Marija. Second group includes Williams, Morettini, Carmen, Hardenpont. As most resistant shown to be Magness, Turandot and two local varietyies Karamanka, as well as another unknown local cultivar. This results are compatible with literature data, but also confirmed observations about susceptibility of various pear cultivars to E. amylovora under natural infections. Artificial inoculation immature pear fruit could be considered as relativelu relible method for estimation of pear fruit trees resistance to this bacteria.
AB  - U radu je prikazana reakcija plodova različitih sorata kruške prema Erwinia amylovora metodom inokulacije nesazrelih plodova. Utvrđeno je da postoje značajne razlike u osetljivosti raznih sorata kruške prema patogenu i one se mogu podeliti u četiri grupe prema korišćenoj skali. Najosetljivijom se pokazala sorta Santa Marija. Nešto manju osetljivost ispoljile su sorte Vilijamova, Moretini, Karmen i Hardenpont; najotpornijim su se pokazale Magnes, Turandot, Junska Lepotica, Karamanka i nepoznata autohtona sorta. Prema dosadašnjim saznanjima ovi rezultati su u saglasnosti sa podacima iz literature i zapažanjima o otpornosti sorti kruške prema E. amylovora, primenom metode inokulacije plodova. Istraživanja su pokazala da se ovakav metod utvrđivanja otpornosti kruške prema E. amylovora sa relativnom pouzdanošću može koristiti u ove svrhe.
PB  - Institute for Plant Protection and Environment, Belgrade
T2  - Zaštita bilja
T1  - Estimation of resistance pear cultivars to Erwinia amylovora using artificial immature pear fruits method
T1  - Ispitivanje otpornosti sorata kruške prema Erwinia amylovora metodom inokulacije nesazrelih plodova
EP  - 123
IS  - 3
SP  - 117
VL  - 65
DO  - 10.5937/zasbilj1403117G
ER  - 

@article{
author = "Gavrilović, Veljko and Stanisavljević, Rade and Stošić, Stefan and Stevanović, Miloš and Aleksić, Goran and Stajić, Milica and Dolovac, Nenad",
year = "2014",
abstract = "Susceptibility of different pear cultivars to Erwinia amaylovora by artificial inoculated immature pear fruits are shown in this article. According obtained results significant differences among cultivars are confirmed and they could be divided in four groups. Most susceptibly cultivars were Santa Marija. Second group includes Williams, Morettini, Carmen, Hardenpont. As most resistant shown to be Magness, Turandot and two local varietyies Karamanka, as well as another unknown local cultivar. This results are compatible with literature data, but also confirmed observations about susceptibility of various pear cultivars to E. amylovora under natural infections. Artificial inoculation immature pear fruit could be considered as relativelu relible method for estimation of pear fruit trees resistance to this bacteria., U radu je prikazana reakcija plodova različitih sorata kruške prema Erwinia amylovora metodom inokulacije nesazrelih plodova. Utvrđeno je da postoje značajne razlike u osetljivosti raznih sorata kruške prema patogenu i one se mogu podeliti u četiri grupe prema korišćenoj skali. Najosetljivijom se pokazala sorta Santa Marija. Nešto manju osetljivost ispoljile su sorte Vilijamova, Moretini, Karmen i Hardenpont; najotpornijim su se pokazale Magnes, Turandot, Junska Lepotica, Karamanka i nepoznata autohtona sorta. Prema dosadašnjim saznanjima ovi rezultati su u saglasnosti sa podacima iz literature i zapažanjima o otpornosti sorti kruške prema E. amylovora, primenom metode inokulacije plodova. Istraživanja su pokazala da se ovakav metod utvrđivanja otpornosti kruške prema E. amylovora sa relativnom pouzdanošću može koristiti u ove svrhe.",
publisher = "Institute for Plant Protection and Environment, Belgrade",
journal = "Zaštita bilja",
title = "Estimation of resistance pear cultivars to Erwinia amylovora using artificial immature pear fruits method, Ispitivanje otpornosti sorata kruške prema Erwinia amylovora metodom inokulacije nesazrelih plodova",
pages = "123-117",
number = "3",
volume = "65",
doi = "10.5937/zasbilj1403117G"
}

Gavrilović, V., Stanisavljević, R., Stošić, S., Stevanović, M., Aleksić, G., Stajić, M.,& Dolovac, N.. (2014). Estimation of resistance pear cultivars to Erwinia amylovora using artificial immature pear fruits method. in Zaštita bilja
Institute for Plant Protection and Environment, Belgrade., 65(3), 117-123.
https://doi.org/10.5937/zasbilj1403117G

Gavrilović V, Stanisavljević R, Stošić S, Stevanović M, Aleksić G, Stajić M, Dolovac N. Estimation of resistance pear cultivars to Erwinia amylovora using artificial immature pear fruits method. in Zaštita bilja. 2014;65(3):117-123.
doi:10.5937/zasbilj1403117G .

Gavrilović, Veljko, Stanisavljević, Rade, Stošić, Stefan, Stevanović, Miloš, Aleksić, Goran, Stajić, Milica, Dolovac, Nenad, "Estimation of resistance pear cultivars to Erwinia amylovora using artificial immature pear fruits method" in Zaštita bilja, 65, no. 3 (2014):117-123,
https://doi.org/10.5937/zasbilj1403117G . .

TY  - JOUR
AU  - Živković, Svetlana
AU  - Gavrilović, Veljko
AU  - Oro, Violeta
AU  - Pfaf-Dolovac, Erika
AU  - Stošić, Stefan
AU  - Kuzmanović, Slobodan
AU  - Ivanović, Žarko
PY  - 2012
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/246
AB  - Isolates of Colletotrichum spp. obtained from nectarine fruits with typical anthracnose symptoms in 2010 were identified using morphological and molecular methods. Pathogenicity test was conducted on symptomless, detached nectarine fruits. All tested isolates caused anthracnose lesions on nectarine fruits after 7 days of incubation. On PDA medium nectarine isolates forming dark green to dark gray mycelia. Growth rates of all isolates and reference strain of C.acutatum were lower at 25°C compared with reference strain of C.gloeosporioides. The conidia were hyaline, aseptate, and fusiform. Appressoria were dark brown, smooth, simple, and clavate to ovate. Using the primer set CaInt2/ITS4, the 490 bp DNA fragment was amplified from all nectarine isolates and reference strain of C. acutatum - CBS 294.67. Based on these results, the causal agent of anthracnose on nectarine fruits in Serbia was identified as C.acutatum.
AB  - Izolati Colletotrichum spp. dobijeni 2010. godine, sa antraknoznih plodova nektarine identifikovani su pomoću morfoloških i molekularnih metoda. Test patogenosti je obavljen sa reprezentativnim izolatima, na odabranim, zdravim plodovima. Svi ispitivani izolati prouzrokuju antraknozne lezije na plodu nektarine, 7 dana nakon inokulacije. Na PDA podlozi izolati formiraju tamno zelenu do tamno sivu miceliju. Stopa rasta izolata sa nektarine i referentnog soja C.acutatum je bila niža u odnosu na referentni soj C.gloeosporioides. Konidije su hialinske, neseptirane i fusiformne. Apresorije su tamno braon boje, glatke, jednostavne, okruglastog ili oblika izdužene palice. Korišćenjem para prajmera CaInt2/ITS4 iz genoma DNA izolata sa ploda nektarine i referentnog soja C. acutatum - CBS 294.67, amplifikovan je fragment veličine 490 bp. Na osnovu ovih rezultata, u Srbiji je kao prouzrokovač antrakoze plodova nektarine identifikovana vrsta C.acutatum.
PB  - Institute for Plant Protection and Environment, Belgrade
T2  - Zaštita bilja
T1  - Identification of Colletotrichum acutatum from nectarine fruit
T1  - Identifikacija Colletotrichum acutatum sa ploda nektarine
EP  - 138
IS  - 3
SP  - 130
VL  - 63
ER  - 

@article{
author = "Živković, Svetlana and Gavrilović, Veljko and Oro, Violeta and Pfaf-Dolovac, Erika and Stošić, Stefan and Kuzmanović, Slobodan and Ivanović, Žarko",
year = "2012",
abstract = "Isolates of Colletotrichum spp. obtained from nectarine fruits with typical anthracnose symptoms in 2010 were identified using morphological and molecular methods. Pathogenicity test was conducted on symptomless, detached nectarine fruits. All tested isolates caused anthracnose lesions on nectarine fruits after 7 days of incubation. On PDA medium nectarine isolates forming dark green to dark gray mycelia. Growth rates of all isolates and reference strain of C.acutatum were lower at 25°C compared with reference strain of C.gloeosporioides. The conidia were hyaline, aseptate, and fusiform. Appressoria were dark brown, smooth, simple, and clavate to ovate. Using the primer set CaInt2/ITS4, the 490 bp DNA fragment was amplified from all nectarine isolates and reference strain of C. acutatum - CBS 294.67. Based on these results, the causal agent of anthracnose on nectarine fruits in Serbia was identified as C.acutatum., Izolati Colletotrichum spp. dobijeni 2010. godine, sa antraknoznih plodova nektarine identifikovani su pomoću morfoloških i molekularnih metoda. Test patogenosti je obavljen sa reprezentativnim izolatima, na odabranim, zdravim plodovima. Svi ispitivani izolati prouzrokuju antraknozne lezije na plodu nektarine, 7 dana nakon inokulacije. Na PDA podlozi izolati formiraju tamno zelenu do tamno sivu miceliju. Stopa rasta izolata sa nektarine i referentnog soja C.acutatum je bila niža u odnosu na referentni soj C.gloeosporioides. Konidije su hialinske, neseptirane i fusiformne. Apresorije su tamno braon boje, glatke, jednostavne, okruglastog ili oblika izdužene palice. Korišćenjem para prajmera CaInt2/ITS4 iz genoma DNA izolata sa ploda nektarine i referentnog soja C. acutatum - CBS 294.67, amplifikovan je fragment veličine 490 bp. Na osnovu ovih rezultata, u Srbiji je kao prouzrokovač antrakoze plodova nektarine identifikovana vrsta C.acutatum.",
publisher = "Institute for Plant Protection and Environment, Belgrade",
journal = "Zaštita bilja",
title = "Identification of Colletotrichum acutatum from nectarine fruit, Identifikacija Colletotrichum acutatum sa ploda nektarine",
pages = "138-130",
number = "3",
volume = "63"
}

Živković, S., Gavrilović, V., Oro, V., Pfaf-Dolovac, E., Stošić, S., Kuzmanović, S.,& Ivanović, Ž.. (2012). Identification of Colletotrichum acutatum from nectarine fruit. in Zaštita bilja
Institute for Plant Protection and Environment, Belgrade., 63(3), 130-138.

Živković S, Gavrilović V, Oro V, Pfaf-Dolovac E, Stošić S, Kuzmanović S, Ivanović Ž. Identification of Colletotrichum acutatum from nectarine fruit. in Zaštita bilja. 2012;63(3):130-138..

Živković, Svetlana, Gavrilović, Veljko, Oro, Violeta, Pfaf-Dolovac, Erika, Stošić, Stefan, Kuzmanović, Slobodan, Ivanović, Žarko, "Identification of Colletotrichum acutatum from nectarine fruit" in Zaštita bilja, 63, no. 3 (2012):130-138.