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Morphological and molecular identification of potato cyst nematode populations in Serbia

Morfološka i molekularna identifikacija populacija cistolikih nematoda krompira u Srbiji

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2010
141.pdf (743.3Kb)
Authors
Oro, Violeta
Ivanović, Žarko
Nikolić, Bogdan
Barši, L.
Radivojević, M.
Jovčić, B.
Article (Published version)
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Abstract
Quarantine species such as potato cyst nematodes Globodera rostochiensis and G. pallida are present in Serbia since 1999 and 2005, respectively. These nematodes are sibling species and their morphological identification is complex due to their morphometric overlap. The cysts from the localities of Kladnica, Šanac, Gojna Gora and Milatovići were grown on susceptible potato varieties and their morphological differences have been discussed. To avoid ambiguities in species morphological designation a duplex PCR method was chosen for a rapid and accurate species identification. The whole procedure, from DNA extraction to DNA isolation, can be performed in a single day.
Karantinske vrste kao što su cistolike nematode krompira Globodera rostochiensis i G. pallida su prisutne u Srbiji od 1999. god. odn. 2005. god. Ove nematode su sestrinske vrste i njihova morfološka identifikacija je složena zbog morfometrijskog preklapanja. Ciste sa lokaliteta Kladnica, Šanac, Gojna Gora i Milatovići su gajene na osetljivim sortama krompira i njihove morfološke razlike su analizirane. Da bi se izbegle nejasnoće u morfološkom određivanju vrsta, izabran je duplex PCR metod za brzu i preciznu specijsku identifikaciju. Celokupna procedura, od ekstrakcije DNK do njene vizualizacije, može biti urađena za jedan dan.
Keywords:
potato cyst nematodes / populations / morphology / duplex PCR
Source:
Archives of Biological Sciences, 2010, 62, 3, 747-754
Publisher:
  • University of Belgrade, University of Novi Sad
Projects:
  • Projekat Ministarstva nauke Republike Srbije, br. 20051: Optimizacija primene hemijskih sredstava u zaštiti bilja povećanjem efikasnosti dijagnostičkih metoda i procene rizika pojave bolesti, štetočina i korova

DOI: 10.2298/ABS1003747O

ISSN: 0354-4664

WoS: 000281482000030

Scopus: 2-s2.0-77955149985
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URI
http://plantarum.izbis.bg.ac.rs/handle/123456789/143
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