Quarantine species such as potato cyst nematodes Globodera rostochiensis and G. pallida are present in Serbia since 1999 and 2005, respectively. These nematodes are sibling species and their morphological identification is complex due to their morphometric overlap. The cysts from the localities of Kladnica, Šanac, Gojna Gora and Milatovići were grown on susceptible potato varieties and their morphological differences have been discussed. To avoid ambiguities in species morphological designation a duplex PCR method was chosen for a rapid and accurate species identification. The whole procedure, from DNA extraction to DNA isolation, can be performed in a single day.
Karantinske vrste kao što su cistolike nematode krompira Globodera rostochiensis i G. pallida su prisutne u Srbiji od 1999. god. odn. 2005. god. Ove nematode su sestrinske vrste i njihova morfološka identifikacija je složena zbog morfometrijskog preklapanja. Ciste sa lokaliteta Kladnica, Šanac, Gojna Gora i Milatovići su gajene na osetljivim sortama krompira i njihove morfološke razlike su analizirane. Da bi se izbegle nejasnoće u morfološkom određivanju vrsta, izabran je duplex PCR metod za brzu i preciznu specijsku identifikaciju. Celokupna procedura, od ekstrakcije DNK do njene vizualizacije, može biti urađena za jedan dan.
Archives of Biological Sciences, 2010, 62, 3, 747-754
Publisher:
University of Belgrade, University of Novi Sad
Projects:
Projekat Ministarstva nauke Republike Srbije, br. 20051: Optimizacija primene hemijskih sredstava u zaštiti bilja povećanjem efikasnosti dijagnostičkih metoda i procene rizika pojave bolesti, štetočina i korova
TY - JOUR
AU - Oro, Violeta
AU - Ivanović, Žarko
AU - Nikolić, Bogdan
AU - Barši, L.
AU - Radivojević, M.
AU - Jovčić, B.
PY - 2010
UR - http://plantarum.izbis.bg.ac.rs/handle/123456789/143
AB - Quarantine species such as potato cyst nematodes Globodera rostochiensis and G. pallida are present in Serbia since 1999 and 2005, respectively. These nematodes are sibling species and their morphological identification is complex due to their morphometric overlap. The cysts from the localities of Kladnica, Šanac, Gojna Gora and Milatovići were grown on susceptible potato varieties and their morphological differences have been discussed. To avoid ambiguities in species morphological designation a duplex PCR method was chosen for a rapid and accurate species identification. The whole procedure, from DNA extraction to DNA isolation, can be performed in a single day.
AB - Karantinske vrste kao što su cistolike nematode krompira Globodera rostochiensis i G. pallida su prisutne u Srbiji od 1999. god. odn. 2005. god. Ove nematode su sestrinske vrste i njihova morfološka identifikacija je složena zbog morfometrijskog preklapanja. Ciste sa lokaliteta Kladnica, Šanac, Gojna Gora i Milatovići su gajene na osetljivim sortama krompira i njihove morfološke razlike su analizirane. Da bi se izbegle nejasnoće u morfološkom određivanju vrsta, izabran je duplex PCR metod za brzu i preciznu specijsku identifikaciju. Celokupna procedura, od ekstrakcije DNK do njene vizualizacije, može biti urađena za jedan dan.
PB - University of Belgrade, University of Novi Sad
T2 - Archives of Biological Sciences
T1 - Morphological and molecular identification of potato cyst nematode populations in Serbia
T1 - Morfološka i molekularna identifikacija populacija cistolikih nematoda krompira u Srbiji
EP - 754
IS - 3
SP - 747
VL - 62
DO - 10.2298/ABS1003747O
UR - conv_74
ER -
@article{
author = "Oro, Violeta and Ivanović, Žarko and Nikolić, Bogdan and Barši, L. and Radivojević, M. and Jovčić, B.",
year = "2010",
abstract = "Quarantine species such as potato cyst nematodes Globodera rostochiensis and G. pallida are present in Serbia since 1999 and 2005, respectively. These nematodes are sibling species and their morphological identification is complex due to their morphometric overlap. The cysts from the localities of Kladnica, Šanac, Gojna Gora and Milatovići were grown on susceptible potato varieties and their morphological differences have been discussed. To avoid ambiguities in species morphological designation a duplex PCR method was chosen for a rapid and accurate species identification. The whole procedure, from DNA extraction to DNA isolation, can be performed in a single day., Karantinske vrste kao što su cistolike nematode krompira Globodera rostochiensis i G. pallida su prisutne u Srbiji od 1999. god. odn. 2005. god. Ove nematode su sestrinske vrste i njihova morfološka identifikacija je složena zbog morfometrijskog preklapanja. Ciste sa lokaliteta Kladnica, Šanac, Gojna Gora i Milatovići su gajene na osetljivim sortama krompira i njihove morfološke razlike su analizirane. Da bi se izbegle nejasnoće u morfološkom određivanju vrsta, izabran je duplex PCR metod za brzu i preciznu specijsku identifikaciju. Celokupna procedura, od ekstrakcije DNK do njene vizualizacije, može biti urađena za jedan dan.",
publisher = "University of Belgrade, University of Novi Sad",
journal = "Archives of Biological Sciences",
title = "Morphological and molecular identification of potato cyst nematode populations in Serbia, Morfološka i molekularna identifikacija populacija cistolikih nematoda krompira u Srbiji",
pages = "754-747",
number = "3",
volume = "62",
doi = "10.2298/ABS1003747O",
url = "conv_74"
}
Oro, V., Ivanović, Ž., Nikolić, B., Barši, L., Radivojević, M.,& Jovčić, B.. (2010). Morphological and molecular identification of potato cyst nematode populations in Serbia. in Archives of Biological Sciences
University of Belgrade, University of Novi Sad., 62(3), 747-754.
https://doi.org/10.2298/ABS1003747O
conv_74
Oro V, Ivanović Ž, Nikolić B, Barši L, Radivojević M, Jovčić B. Morphological and molecular identification of potato cyst nematode populations in Serbia. in Archives of Biological Sciences. 2010;62(3):747-754.
doi:10.2298/ABS1003747O
conv_74 .
Oro, Violeta, Ivanović, Žarko, Nikolić, Bogdan, Barši, L., Radivojević, M., Jovčić, B., "Morphological and molecular identification of potato cyst nematode populations in Serbia" in Archives of Biological Sciences, 62, no. 3 (2010):747-754,
https://doi.org/10.2298/ABS1003747O .,
conv_74 .
