Kuzmanović, S., Starović, M., Ristić, D., Vučurović, A., Kuzmanović, N., Trkulja, N.,& Aleksić, G.. (2024). Сорта Пловдина – нова индикатор биљка за детекцију фитоплазми винове лозе. in Институт за заштиту биља и животну средину
Институт за заштиту биља и животну средину..

Kuzmanović S, Starović M, Ristić D, Vučurović A, Kuzmanović N, Trkulja N, Aleksić G. Сорта Пловдина – нова индикатор биљка за детекцију фитоплазми винове лозе. in Институт за заштиту биља и животну средину. 2024;..

Kuzmanović, Slobodan, Starović, Mira, Ristić, Danijela, Vučurović, Ana, Kuzmanović, Nemanja, Trkulja, Nenad, Aleksić, Goran, "Сорта Пловдина – нова индикатор биљка за детекцију фитоплазми винове лозе" in Институт за заштиту биља и животну средину (2024).

TY  - CONF
AU  - Keserović, Vukašin
AU  - Starović, Mira
AU  - Ristić, Danijela
AU  - Vučurović, Ivan
AU  - Blagojević, Jovana
AU  - Aleksic, Goran
PY  - 2023
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/1206
AB  - Didymella pinodella – prozrokovač crne pegavosti graška je najrazornija bolest graška
širom sveta koja, u zavisnosti od uslova sredine i kvaliteta graška, može usloviti gubitke
prinosa od 28 do 88%.
U ovom radu, u in vitro uslovima, ispitan je uticaj fungicida različitog mehanizma
delovanja na izolate D. pinodella poreklom sa graška.
Izolati D. pinodella korišćeni u ispitivanjima, poreklom su sa lista i mahuna graška,
kolekcionisani tokom 2023 godine iz lokaliteta Trbušac i Skupljen (okolina Šapca) i deo su
kolekcije fitopatogenih gljiva Instituta za zaštitu bilja i životnu sredinu (Beograd). Ispitivana je
osetljivost gljive prema četiri kombinacije komercijalnih fungicida: ciprodinil + fludioksonil,
fluksapiroksad + difenokonazol, azoksistrobin + difenokonazol i difenokonazol a kao standard
je korišćen azoksistrobin. Primenjeni su fungicidi u početnoj preporučenoj koncentraciji
primene: ciprodinil + fludioksonil (25μg/10ml vode), fluksapiroksad + difenokonazol
(100μl/10ml vode), azoksistrobin + difenokonazol (50μl/10ml vode), difenokonazol
(25μl/10ml vode) i azoksistrobin (50μl/10ml vode). Za ispitivanje antifungalne aktivnosti
fungicida korišćena je mikrodiluciona metoda na miktrotitarskim pločama. Određivanje
minimalnih inhibitornih koncentracija (MIC) vršeno je pomoću skale razređenja primenjenih
fungicida od 10-1 do 10-9 u krompir dekstroznom tečnom medijumu u četiri ponavljanja. Na
kraju je dodavano 10 μl suspenzije micelije u tečni medijum finalne zapremine od 100 μl.
Mikrotitarske pločice inkubirane su u termostatu na 25°C u trajanju od 14 dana. Najniža
koncentracija na kojoj nije bilo rasta micelije uzimana je za minimalnu inhibitornu
koncentraciju (MIC).
Najviši stepen tokstičnosti ispoljila je kombinacija azoksistrobina i difenokonazola, sa
prosečnom vrednosti minimalne koncentracije inhibicije od 0,003 μl/ml; zatim kombinacija
ciprodinila i fludioksonila - MIC 0,02 μg/ml; kombinacija fluksapiroksada i difenokonazola -
MIC 0,08 μl/ml, dok je najnižu toksičnost ispoljio difenokonazol - MIC 0,2 μl/ml.
PB  - Društvo za zaštitu bilja
C3  - XVII simpozijum o zaštiti bilja, Zlatibor 2023, 27-30. novembar, Zbornik rezimea radova
T1  - Toksičnost fungicida različitog mehanizma delovanja prema izolatima Didymella pinodella poreklom sa graška
SP  - 84
ER  - 

@conference{
author = "Keserović, Vukašin and Starović, Mira and Ristić, Danijela and Vučurović, Ivan and Blagojević, Jovana and Aleksic, Goran",
year = "2023",
abstract = "Didymella pinodella – prozrokovač crne pegavosti graška je najrazornija bolest graška
širom sveta koja, u zavisnosti od uslova sredine i kvaliteta graška, može usloviti gubitke
prinosa od 28 do 88%.
U ovom radu, u in vitro uslovima, ispitan je uticaj fungicida različitog mehanizma
delovanja na izolate D. pinodella poreklom sa graška.
Izolati D. pinodella korišćeni u ispitivanjima, poreklom su sa lista i mahuna graška,
kolekcionisani tokom 2023 godine iz lokaliteta Trbušac i Skupljen (okolina Šapca) i deo su
kolekcije fitopatogenih gljiva Instituta za zaštitu bilja i životnu sredinu (Beograd). Ispitivana je
osetljivost gljive prema četiri kombinacije komercijalnih fungicida: ciprodinil + fludioksonil,
fluksapiroksad + difenokonazol, azoksistrobin + difenokonazol i difenokonazol a kao standard
je korišćen azoksistrobin. Primenjeni su fungicidi u početnoj preporučenoj koncentraciji
primene: ciprodinil + fludioksonil (25μg/10ml vode), fluksapiroksad + difenokonazol
(100μl/10ml vode), azoksistrobin + difenokonazol (50μl/10ml vode), difenokonazol
(25μl/10ml vode) i azoksistrobin (50μl/10ml vode). Za ispitivanje antifungalne aktivnosti
fungicida korišćena je mikrodiluciona metoda na miktrotitarskim pločama. Određivanje
minimalnih inhibitornih koncentracija (MIC) vršeno je pomoću skale razređenja primenjenih
fungicida od 10-1 do 10-9 u krompir dekstroznom tečnom medijumu u četiri ponavljanja. Na
kraju je dodavano 10 μl suspenzije micelije u tečni medijum finalne zapremine od 100 μl.
Mikrotitarske pločice inkubirane su u termostatu na 25°C u trajanju od 14 dana. Najniža
koncentracija na kojoj nije bilo rasta micelije uzimana je za minimalnu inhibitornu
koncentraciju (MIC).
Najviši stepen tokstičnosti ispoljila je kombinacija azoksistrobina i difenokonazola, sa
prosečnom vrednosti minimalne koncentracije inhibicije od 0,003 μl/ml; zatim kombinacija
ciprodinila i fludioksonila - MIC 0,02 μg/ml; kombinacija fluksapiroksada i difenokonazola -
MIC 0,08 μl/ml, dok je najnižu toksičnost ispoljio difenokonazol - MIC 0,2 μl/ml.",
publisher = "Društvo za zaštitu bilja",
journal = "XVII simpozijum o zaštiti bilja, Zlatibor 2023, 27-30. novembar, Zbornik rezimea radova",
title = "Toksičnost fungicida različitog mehanizma delovanja prema izolatima Didymella pinodella poreklom sa graška",
pages = "84"
}

Keserović, V., Starović, M., Ristić, D., Vučurović, I., Blagojević, J.,& Aleksic, G.. (2023). Toksičnost fungicida različitog mehanizma delovanja prema izolatima Didymella pinodella poreklom sa graška. in XVII simpozijum o zaštiti bilja, Zlatibor 2023, 27-30. novembar, Zbornik rezimea radova
Društvo za zaštitu bilja., 84.

Keserović V, Starović M, Ristić D, Vučurović I, Blagojević J, Aleksic G. Toksičnost fungicida različitog mehanizma delovanja prema izolatima Didymella pinodella poreklom sa graška. in XVII simpozijum o zaštiti bilja, Zlatibor 2023, 27-30. novembar, Zbornik rezimea radova. 2023;:84..

Keserović, Vukašin, Starović, Mira, Ristić, Danijela, Vučurović, Ivan, Blagojević, Jovana, Aleksic, Goran, "Toksičnost fungicida različitog mehanizma delovanja prema izolatima Didymella pinodella poreklom sa graška" in XVII simpozijum o zaštiti bilja, Zlatibor 2023, 27-30. novembar, Zbornik rezimea radova (2023):84.

TY  - JOUR
AU  - Blagojević, Jovana
AU  - Aleksic, Goran
AU  - Vučurović, Ivan
AU  - Starović, Mira
AU  - Ristić, Danijela
PY  - 2023
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/1180
AB  - Identifying the precise pathogens responsible for specific plant diseases is imperative for implementing targeted and efficient interventions and mitigating their spread. Dieback and shoot blight significantly diminish the lifespan and productivity of blueberries, yet the causative agents remain largely unidentified. To determine the identity and prevalence of the causal agents of branch dieback and shoot blight, we conducted multi-year and multi-site sampling of diseased highbush blueberries (Vaccinium corymbosum cv. Duke) in Serbia. Sixty-nine monosporic isolates were collected and characterized based on morphological, physiological features and multi-locus phylogenetic analysis of internal transcribed spacer (ITS), β-tubulin (TUB2) and translation elongation factor 1-alpha (TEF1) sequence data. Five species were identified as causal agents: Diaporthe eres (36 isolates), D. foeniculina (3 isolates), Neopestalotiopsis vaccinii (9 isolates), N. rosae (6 isolates) and Neofusicoccum parvum (15 isolates). The results of the pathogenicity tests performed with the 23 representative isolates confirmed the role of these species as primary pathogens in causing dieback and shoot blight of blueberry, with N. parvum being the most aggressive and D. eres the least. Our study underscores the diversity of genera and species of ascomycetes capable of causing blueberry dieback and shoot blight. Furthermore, our findings indicate that the agents responsible for the disease in Serbia differ from those identified in other regions worldwide.
PB  - The American Phytopathological Society
T2  - Phytopathology
T1  - Exploring the phylogenetic diversity of Botryosphaeriaceae and Diaporthe species causing dieback and shoot blight of blueberry in Serbia
DO  - https://doi.org/10.1094/PHYTO-04-23-0133-R
ER  - 

@article{
author = "Blagojević, Jovana and Aleksic, Goran and Vučurović, Ivan and Starović, Mira and Ristić, Danijela",
year = "2023",
abstract = "Identifying the precise pathogens responsible for specific plant diseases is imperative for implementing targeted and efficient interventions and mitigating their spread. Dieback and shoot blight significantly diminish the lifespan and productivity of blueberries, yet the causative agents remain largely unidentified. To determine the identity and prevalence of the causal agents of branch dieback and shoot blight, we conducted multi-year and multi-site sampling of diseased highbush blueberries (Vaccinium corymbosum cv. Duke) in Serbia. Sixty-nine monosporic isolates were collected and characterized based on morphological, physiological features and multi-locus phylogenetic analysis of internal transcribed spacer (ITS), β-tubulin (TUB2) and translation elongation factor 1-alpha (TEF1) sequence data. Five species were identified as causal agents: Diaporthe eres (36 isolates), D. foeniculina (3 isolates), Neopestalotiopsis vaccinii (9 isolates), N. rosae (6 isolates) and Neofusicoccum parvum (15 isolates). The results of the pathogenicity tests performed with the 23 representative isolates confirmed the role of these species as primary pathogens in causing dieback and shoot blight of blueberry, with N. parvum being the most aggressive and D. eres the least. Our study underscores the diversity of genera and species of ascomycetes capable of causing blueberry dieback and shoot blight. Furthermore, our findings indicate that the agents responsible for the disease in Serbia differ from those identified in other regions worldwide.",
publisher = "The American Phytopathological Society",
journal = "Phytopathology",
title = "Exploring the phylogenetic diversity of Botryosphaeriaceae and Diaporthe species causing dieback and shoot blight of blueberry in Serbia",
doi = "https://doi.org/10.1094/PHYTO-04-23-0133-R"
}

Blagojević, J., Aleksic, G., Vučurović, I., Starović, M.,& Ristić, D.. (2023). Exploring the phylogenetic diversity of Botryosphaeriaceae and Diaporthe species causing dieback and shoot blight of blueberry in Serbia. in Phytopathology
The American Phytopathological Society..
https://doi.org/https://doi.org/10.1094/PHYTO-04-23-0133-R

Blagojević J, Aleksic G, Vučurović I, Starović M, Ristić D. Exploring the phylogenetic diversity of Botryosphaeriaceae and Diaporthe species causing dieback and shoot blight of blueberry in Serbia. in Phytopathology. 2023;.
doi:https://doi.org/10.1094/PHYTO-04-23-0133-R .

Blagojević, Jovana, Aleksic, Goran, Vučurović, Ivan, Starović, Mira, Ristić, Danijela, "Exploring the phylogenetic diversity of Botryosphaeriaceae and Diaporthe species causing dieback and shoot blight of blueberry in Serbia" in Phytopathology (2023),
https://doi.org/https://doi.org/10.1094/PHYTO-04-23-0133-R . .

TY  - CONF
AU  - Popović Milovanović, Tatjana
AU  - Trkulja, Nenad
AU  - Ristić, Danijela
AU  - Iličić, Renata
AU  - Trkulja, Vojislav
AU  - Jelušić, Aleksandra
PY  - 2023
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/1197
AB  - Bakteriozna pegavost lista šećerne repe prouzrokovana fitopatogenom bakterijom
Pseudomonas syringae pv. aptata (Psa) je široko rasprostranjena bolest, koja osim ove biljne
kulture napada blitvu, cveklu i dr. Ova bolest se poslednjih godina sve češće javlja na usevima
šećerne repe u Srbiji, posebno u uslovima prohladnog i vlažnog proleća. Epifitocije su
beležene 2013. godine u centralnim i južnim delovima Vojvodine (Bačka i Srem), kada su na
osnovu sekvenciranja konzervativnog gena gyrB, utvrđene četiri različite alelske varijante
ovog patogena. Obzirom na noviju epifitociju bakteriozne pegavosti lista na području gajenja
šećerne repe u Srbiji, cilj ovoga rada je bio da se izvrši izolacija i identifikacija prouzrokovača i
determiniše njegov genetički diverzitet na osnovu analize ponavljajućih palindromskih
sekvenci (rep-PCR) i nasumično amplifikovanih polimorfnih DNK (RAPD-PCR).
Tokom 2023. godine na šećernoj repi (sorta Viola) u lokalitetima Ruski Krstur i Srpski
Miletić prikupljeni su uzorci obolelog lišća sa simptomima bakteriozne pegavosti u vidu
nepravilnih nekrotičnih pega, oivičenih tamnijim marginama, sa centralnim delom mrke do
sive boje. U nekim slučajevima pege su se spajale čineći veću nekrotičnu leziju. Izolacija je
vršena na hranljivu podlogu obogaćenu sa 5% saharoze (NAS), na kojoj su nakon tri dana
inkubacije na 26 °C, dominirale beličaste, okrugle, sjajne i ispupčene bakterijske kolonije. Za
dalji rad je odabrano 18 izolata, a kao uporedni poslužili su referentni Psa sojevi, PD193 i
PD197. Prema rezultatima LOPAT testova, svi izolati su pokazali pripadnost Ia grupi
fluorescentnih Pseudomonas (+---+). Patogenost je dokazana infiltracijom bakterijske
suspenzije u list šećerne repe. Pripadnost izolata bakteriji Psa dokazana je PCR metodom
primenom prajmera specifičnih za patovar Papt2F/1R kojima su amplifikovani fragmenti DNK
veličine 250 bp karakteristični za Psa. Rep-PCR je izvođen primenom prajmera BOXA1R (BOXPCR), ERIC1R/ERIC2 (ERIC-PCR) i GTG5 (GTG5-PCR), dok je RAPD-PCR rađen primenom
prajmera M13 za M13-PCR. Na osnovu analize DNK profila, dobijenih primenom dve
pomenute metode, dokazano je postojanje tri (GTG5-PCR), odnosno četiri (BOX-, ERIC- i M13-
PCR) genetički različite grupe izolata. Dobijeni rezultati su ukazali na postojanje genetičkog
diverziteta i kod novijih populacija bakterije Psa poreklom sa šećerne repe. Daljom analizom
sekvenci više genskih lokusa (MLSA) moguće je detaljnije utvrditi eventualno postojanje
novih populacija ove bakterije u Srbiji.
PB  - Društvo za zaštitu bilja
C3  - XVII simpozijum o zaštiti bilja, Zlatibor, 2023 27-30. novembar, Zbornik rezimea radova
T1  - Novija proučavanja uzročnika bakteriozne pegavosti lista šećerne repe
SP  - 49
ER  - 

@conference{
author = "Popović Milovanović, Tatjana and Trkulja, Nenad and Ristić, Danijela and Iličić, Renata and Trkulja, Vojislav and Jelušić, Aleksandra",
year = "2023",
abstract = "Bakteriozna pegavost lista šećerne repe prouzrokovana fitopatogenom bakterijom
Pseudomonas syringae pv. aptata (Psa) je široko rasprostranjena bolest, koja osim ove biljne
kulture napada blitvu, cveklu i dr. Ova bolest se poslednjih godina sve češće javlja na usevima
šećerne repe u Srbiji, posebno u uslovima prohladnog i vlažnog proleća. Epifitocije su
beležene 2013. godine u centralnim i južnim delovima Vojvodine (Bačka i Srem), kada su na
osnovu sekvenciranja konzervativnog gena gyrB, utvrđene četiri različite alelske varijante
ovog patogena. Obzirom na noviju epifitociju bakteriozne pegavosti lista na području gajenja
šećerne repe u Srbiji, cilj ovoga rada je bio da se izvrši izolacija i identifikacija prouzrokovača i
determiniše njegov genetički diverzitet na osnovu analize ponavljajućih palindromskih
sekvenci (rep-PCR) i nasumično amplifikovanih polimorfnih DNK (RAPD-PCR).
Tokom 2023. godine na šećernoj repi (sorta Viola) u lokalitetima Ruski Krstur i Srpski
Miletić prikupljeni su uzorci obolelog lišća sa simptomima bakteriozne pegavosti u vidu
nepravilnih nekrotičnih pega, oivičenih tamnijim marginama, sa centralnim delom mrke do
sive boje. U nekim slučajevima pege su se spajale čineći veću nekrotičnu leziju. Izolacija je
vršena na hranljivu podlogu obogaćenu sa 5% saharoze (NAS), na kojoj su nakon tri dana
inkubacije na 26 °C, dominirale beličaste, okrugle, sjajne i ispupčene bakterijske kolonije. Za
dalji rad je odabrano 18 izolata, a kao uporedni poslužili su referentni Psa sojevi, PD193 i
PD197. Prema rezultatima LOPAT testova, svi izolati su pokazali pripadnost Ia grupi
fluorescentnih Pseudomonas (+---+). Patogenost je dokazana infiltracijom bakterijske
suspenzije u list šećerne repe. Pripadnost izolata bakteriji Psa dokazana je PCR metodom
primenom prajmera specifičnih za patovar Papt2F/1R kojima su amplifikovani fragmenti DNK
veličine 250 bp karakteristični za Psa. Rep-PCR je izvođen primenom prajmera BOXA1R (BOXPCR), ERIC1R/ERIC2 (ERIC-PCR) i GTG5 (GTG5-PCR), dok je RAPD-PCR rađen primenom
prajmera M13 za M13-PCR. Na osnovu analize DNK profila, dobijenih primenom dve
pomenute metode, dokazano je postojanje tri (GTG5-PCR), odnosno četiri (BOX-, ERIC- i M13-
PCR) genetički različite grupe izolata. Dobijeni rezultati su ukazali na postojanje genetičkog
diverziteta i kod novijih populacija bakterije Psa poreklom sa šećerne repe. Daljom analizom
sekvenci više genskih lokusa (MLSA) moguće je detaljnije utvrditi eventualno postojanje
novih populacija ove bakterije u Srbiji.",
publisher = "Društvo za zaštitu bilja",
journal = "XVII simpozijum o zaštiti bilja, Zlatibor, 2023 27-30. novembar, Zbornik rezimea radova",
title = "Novija proučavanja uzročnika bakteriozne pegavosti lista šećerne repe",
pages = "49"
}

Popović Milovanović, T., Trkulja, N., Ristić, D., Iličić, R., Trkulja, V.,& Jelušić, A.. (2023). Novija proučavanja uzročnika bakteriozne pegavosti lista šećerne repe. in XVII simpozijum o zaštiti bilja, Zlatibor, 2023 27-30. novembar, Zbornik rezimea radova
Društvo za zaštitu bilja., 49.

Popović Milovanović T, Trkulja N, Ristić D, Iličić R, Trkulja V, Jelušić A. Novija proučavanja uzročnika bakteriozne pegavosti lista šećerne repe. in XVII simpozijum o zaštiti bilja, Zlatibor, 2023 27-30. novembar, Zbornik rezimea radova. 2023;:49..

Popović Milovanović, Tatjana, Trkulja, Nenad, Ristić, Danijela, Iličić, Renata, Trkulja, Vojislav, Jelušić, Aleksandra, "Novija proučavanja uzročnika bakteriozne pegavosti lista šećerne repe" in XVII simpozijum o zaštiti bilja, Zlatibor, 2023 27-30. novembar, Zbornik rezimea radova (2023):49.

TY  - CONF
AU  - Zečević, Katarina
AU  - Stanković, Ivana
AU  - Ristić, Danijela
AU  - Vučurović, Ivan
AU  - Krstić, Branka
PY  - 2023
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/1207
C3  - XIV International Agriculture Symposium "Agrosym 2023", October 5-8, 2023, Jahorina, Bosnia and Herzegovina
T1  - FIRST REPORT OF CUCUMBER MOSAIC VIRUS INFECTING PAEONIA HYBRIDA IN SERBIA
ER  - 

@conference{
author = "Zečević, Katarina and Stanković, Ivana and Ristić, Danijela and Vučurović, Ivan and Krstić, Branka",
year = "2023",
journal = "XIV International Agriculture Symposium "Agrosym 2023", October 5-8, 2023, Jahorina, Bosnia and Herzegovina",
title = "FIRST REPORT OF CUCUMBER MOSAIC VIRUS INFECTING PAEONIA HYBRIDA IN SERBIA"
}

Zečević, K., Stanković, I., Ristić, D., Vučurović, I.,& Krstić, B.. (2023). FIRST REPORT OF CUCUMBER MOSAIC VIRUS INFECTING PAEONIA HYBRIDA IN SERBIA. in XIV International Agriculture Symposium "Agrosym 2023", October 5-8, 2023, Jahorina, Bosnia and Herzegovina.

Zečević K, Stanković I, Ristić D, Vučurović I, Krstić B. FIRST REPORT OF CUCUMBER MOSAIC VIRUS INFECTING PAEONIA HYBRIDA IN SERBIA. in XIV International Agriculture Symposium "Agrosym 2023", October 5-8, 2023, Jahorina, Bosnia and Herzegovina. 2023;..

Zečević, Katarina, Stanković, Ivana, Ristić, Danijela, Vučurović, Ivan, Krstić, Branka, "FIRST REPORT OF CUCUMBER MOSAIC VIRUS INFECTING PAEONIA HYBRIDA IN SERBIA" in XIV International Agriculture Symposium "Agrosym 2023", October 5-8, 2023, Jahorina, Bosnia and Herzegovina (2023).

Ristić, D., Vučurović, I., Starović, M., Aleksic, G., Štrbanović, R., Đurović, S.,& Nikolić, B.. (2022). Primena različitih kombinacija smeša mlečno-kiselinskih, fotofobnih bakterija i kvasaca u suzbijanju patogena semena i klijanaca paradajza i paprika. in Institut za zaštitu bilja i životnu sredinu
Institut za zaštitu bilja i životnu sredinu, Beograd..

Ristić D, Vučurović I, Starović M, Aleksic G, Štrbanović R, Đurović S, Nikolić B. Primena različitih kombinacija smeša mlečno-kiselinskih, fotofobnih bakterija i kvasaca u suzbijanju patogena semena i klijanaca paradajza i paprika. in Institut za zaštitu bilja i životnu sredinu. 2022;..

Ristić, Danijela, Vučurović, Ivan, Starović, Mira, Aleksic, Goran, Štrbanović, Ratibor, Đurović, Sanja, Nikolić, Bogdan, "Primena različitih kombinacija smeša mlečno-kiselinskih, fotofobnih bakterija i kvasaca u suzbijanju patogena semena i klijanaca paradajza i paprika" in Institut za zaštitu bilja i životnu sredinu (2022).

TY  - CONF
AU  - Zivkovic, Svetlana
AU  - Ristić, Lela
AU  - Starović, Mira
AU  - Aleksic, Goran
AU  - Stošić, Stefan
PY  - 2022
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/791
AB  - Tomato (Solanum lycopersicum, L.) is one of the most widely cultivated crops with high content of vitamins and antioxidant lycopene, which are very important for human health. During the growing season and postharvest storage tomato is susceptible to various diseases caused by pathogenic fungi. In July 2019, tomato (cv. Balkan) with symptoms of blue mold decay were collected from market in Belgrade, Serbia. Macroscopic morphology of three obtained monosporic isolates were observed after growth on Czapek yeast autolysate agar (CYA), creatine sucrose agar (CREA), and malt extract agar (MEA) for seven days at 25ºC. Also, selected isolates were incubated at 5, 25, and 37°C for one week on CYA to monitor the effect of different temperature incubation conditions. Colony characteristics and micromorphology of the fungi agreed with the literature descriptions of Penicillium expansum. The conidiophores of isolates were hyaline, mainly terverticillate; stipes usually smooth-walled; metulae and phialides cylindrical; conidia ellipsoidal (3-3,86-4 × 3-3,13-4 μm), greenish, smooth-walled. Total DNA was extracted using DNeasy Plant Mini Kit (Qiagen, Hilden, Germany) and partial β-tubulin (BenA) sequence was amplified with primers Bt2a/Bt2b. BenA sequence of representative isolate ParP/1 was deposited in NCBI GenBank (Accession No. ON186699). Phylogenetic analysis clustered our isolate with other isolates of P. expansum. Pathogenicity test was conducted on symptomless, detached tomato fruits. All tested isolates caused typical blue mold symptoms on tomato fruits after seven days of incubation. To our knowledge, this is the first report of P. expansum causing postharvest fruit decay on tomato in Serbia.
PB  - Univerisity of East Sarajevo, Faculty of Agriculture
C3  - Proceedings of the XIII International Scientific Agricultural Symposium “Agrosym 2022” 6-9 oktobar 2022, 545 - 551
T1  - Penicillium expansum as a postharvest pathogen of tomato fruit in Serbia
ER  - 

@conference{
author = "Zivkovic, Svetlana and Ristić, Lela and Starović, Mira and Aleksic, Goran and Stošić, Stefan",
year = "2022",
abstract = "Tomato (Solanum lycopersicum, L.) is one of the most widely cultivated crops with high content of vitamins and antioxidant lycopene, which are very important for human health. During the growing season and postharvest storage tomato is susceptible to various diseases caused by pathogenic fungi. In July 2019, tomato (cv. Balkan) with symptoms of blue mold decay were collected from market in Belgrade, Serbia. Macroscopic morphology of three obtained monosporic isolates were observed after growth on Czapek yeast autolysate agar (CYA), creatine sucrose agar (CREA), and malt extract agar (MEA) for seven days at 25ºC. Also, selected isolates were incubated at 5, 25, and 37°C for one week on CYA to monitor the effect of different temperature incubation conditions. Colony characteristics and micromorphology of the fungi agreed with the literature descriptions of Penicillium expansum. The conidiophores of isolates were hyaline, mainly terverticillate; stipes usually smooth-walled; metulae and phialides cylindrical; conidia ellipsoidal (3-3,86-4 × 3-3,13-4 μm), greenish, smooth-walled. Total DNA was extracted using DNeasy Plant Mini Kit (Qiagen, Hilden, Germany) and partial β-tubulin (BenA) sequence was amplified with primers Bt2a/Bt2b. BenA sequence of representative isolate ParP/1 was deposited in NCBI GenBank (Accession No. ON186699). Phylogenetic analysis clustered our isolate with other isolates of P. expansum. Pathogenicity test was conducted on symptomless, detached tomato fruits. All tested isolates caused typical blue mold symptoms on tomato fruits after seven days of incubation. To our knowledge, this is the first report of P. expansum causing postharvest fruit decay on tomato in Serbia.",
publisher = "Univerisity of East Sarajevo, Faculty of Agriculture",
journal = "Proceedings of the XIII International Scientific Agricultural Symposium “Agrosym 2022” 6-9 oktobar 2022, 545 - 551",
title = "Penicillium expansum as a postharvest pathogen of tomato fruit in Serbia"
}

Zivkovic, S., Ristić, L., Starović, M., Aleksic, G.,& Stošić, S.. (2022). Penicillium expansum as a postharvest pathogen of tomato fruit in Serbia. in Proceedings of the XIII International Scientific Agricultural Symposium “Agrosym 2022” 6-9 oktobar 2022, 545 - 551
Univerisity of East Sarajevo, Faculty of Agriculture..

Zivkovic S, Ristić L, Starović M, Aleksic G, Stošić S. Penicillium expansum as a postharvest pathogen of tomato fruit in Serbia. in Proceedings of the XIII International Scientific Agricultural Symposium “Agrosym 2022” 6-9 oktobar 2022, 545 - 551. 2022;..

Zivkovic, Svetlana, Ristić, Lela, Starović, Mira, Aleksic, Goran, Stošić, Stefan, "Penicillium expansum as a postharvest pathogen of tomato fruit in Serbia" in Proceedings of the XIII International Scientific Agricultural Symposium “Agrosym 2022” 6-9 oktobar 2022, 545 - 551 (2022).

TY  - CONF
AU  - Starović, Mira
AU  - Ristić, Lela
AU  - Vučurović, Ivan
AU  - Blagojević, Jovana
AU  - Stošić, Stefan
AU  - Živković, Svetlana
AU  - Aleksic, Goran
PY  - 2022
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/787
AB  - Garlic (Allium sativum L.) is the second most cultivated Allium species worldwide. Many viral, fungal and bacterial diseases attack garlic plants and can be a limiting factor to garlic production. Fungal diseases control is mainly conducted by fungicides, which have detrimental impact on the environment. In this work, we explore the possibility of utilizing essential oils (EOs) as antifungal agents against a fungal isolate originated from symptomatic garlic cloves from Kraljevci locality, Srem District, Serbia, morphologically and molecularly identified as Fusarium verticillioides. EOs used in this study originated from several medicinal plants: Turkish pickling herb (Echinophora tenuifolia), oregano (Origanum vulgare), basil (Ocimum basilicum) and myrtle (Myrtus communis). Minimum inhibitory concentrations (MIC) were determined by microdilution method in 96 well microtiter plates. Microtiter plates were incubated for five days at 28°C. The experiment was repeated four times with trifloxystrobin as a positive control. The lowest concentrations without visible growth were defined as the minimal concentrations inhibiting fungal growth. Fungal spores were washed from the surface of potato dextrose agar (PDA) and spore suspension was adjusted to a concentration of approximately 5.0x104 in a final volume of 100μl per well. The values of minimal inhibitory concentration (MIC) were carried out by Duncan’s multiple range tests. An analysis of variance was performed on MIC data for four EOs applied on F. verticillioides. A significance was evaluated at p<0.05. STATISTICA v.7 (StatSoft, Inc.) was used for statistical analyses. The results of the antimicrobial activity of EOs using microdilution method showed a wide range of antifungal activity against F. verticillioides. The basil EO proved to be the most potent one (MIC-0,325±5,10μg/mL), followed by oregano (MIC-0,775±0,05μg/mL), myrtle (MIC-5,5±0,05μg/mL) and Turkish pickling herb (MIC-55±5,10μg/mL). The data obtained here suggest that the selected EOs can be applied as inhibitors to prevent growth of the phytopathogenic fungus F. verticillioides.
PB  - University of East Sarajevo
C3  - Proceedings of the XIII International Scientific Agricultural Symposium “Agrosym October 6-9, 2022
T1  - Antifungal activity of plant essential oils to the Fusarium verticillioides originated from garlic
EP  - 650
IS  - 645
ER  - 

@conference{
author = "Starović, Mira and Ristić, Lela and Vučurović, Ivan and Blagojević, Jovana and Stošić, Stefan and Živković, Svetlana and Aleksic, Goran",
year = "2022",
abstract = "Garlic (Allium sativum L.) is the second most cultivated Allium species worldwide. Many viral, fungal and bacterial diseases attack garlic plants and can be a limiting factor to garlic production. Fungal diseases control is mainly conducted by fungicides, which have detrimental impact on the environment. In this work, we explore the possibility of utilizing essential oils (EOs) as antifungal agents against a fungal isolate originated from symptomatic garlic cloves from Kraljevci locality, Srem District, Serbia, morphologically and molecularly identified as Fusarium verticillioides. EOs used in this study originated from several medicinal plants: Turkish pickling herb (Echinophora tenuifolia), oregano (Origanum vulgare), basil (Ocimum basilicum) and myrtle (Myrtus communis). Minimum inhibitory concentrations (MIC) were determined by microdilution method in 96 well microtiter plates. Microtiter plates were incubated for five days at 28°C. The experiment was repeated four times with trifloxystrobin as a positive control. The lowest concentrations without visible growth were defined as the minimal concentrations inhibiting fungal growth. Fungal spores were washed from the surface of potato dextrose agar (PDA) and spore suspension was adjusted to a concentration of approximately 5.0x104 in a final volume of 100μl per well. The values of minimal inhibitory concentration (MIC) were carried out by Duncan’s multiple range tests. An analysis of variance was performed on MIC data for four EOs applied on F. verticillioides. A significance was evaluated at p<0.05. STATISTICA v.7 (StatSoft, Inc.) was used for statistical analyses. The results of the antimicrobial activity of EOs using microdilution method showed a wide range of antifungal activity against F. verticillioides. The basil EO proved to be the most potent one (MIC-0,325±5,10μg/mL), followed by oregano (MIC-0,775±0,05μg/mL), myrtle (MIC-5,5±0,05μg/mL) and Turkish pickling herb (MIC-55±5,10μg/mL). The data obtained here suggest that the selected EOs can be applied as inhibitors to prevent growth of the phytopathogenic fungus F. verticillioides.",
publisher = "University of East Sarajevo",
journal = "Proceedings of the XIII International Scientific Agricultural Symposium “Agrosym October 6-9, 2022",
title = "Antifungal activity of plant essential oils to the Fusarium verticillioides originated from garlic",
pages = "650",
number = "645"
}

Starović, M., Ristić, L., Vučurović, I., Blagojević, J., Stošić, S., Živković, S.,& Aleksic, G.. (2022). Antifungal activity of plant essential oils to the Fusarium verticillioides originated from garlic. in Proceedings of the XIII International Scientific Agricultural Symposium “Agrosym October 6-9, 2022
University of East Sarajevo.(645).

Starović M, Ristić L, Vučurović I, Blagojević J, Stošić S, Živković S, Aleksic G. Antifungal activity of plant essential oils to the Fusarium verticillioides originated from garlic. in Proceedings of the XIII International Scientific Agricultural Symposium “Agrosym October 6-9, 2022. 2022;(645):null-650..

Starović, Mira, Ristić, Lela, Vučurović, Ivan, Blagojević, Jovana, Stošić, Stefan, Živković, Svetlana, Aleksic, Goran, "Antifungal activity of plant essential oils to the Fusarium verticillioides originated from garlic" in Proceedings of the XIII International Scientific Agricultural Symposium “Agrosym October 6-9, 2022, no. 645 (2022).

TY  - JOUR
AU  - Gašić, Katarina
AU  - Obradović, Mina
AU  - Kuzmanović, Nemanja
AU  - Zlatković, Nevena
AU  - Ivanović, Milan
AU  - Ristić, Lela
AU  - Obradović, Aleksa
PY  - 2022
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/725
AB  - Bacterial fruit blotch and seedling blight, caused by Acidovorax citrulli, is one of the most destructive diseases of melon and watermelon in many countries. Pathogen-free seed and cultural practices are major pillars of the disease control. However, use of bacteriophages as natural biocontrol agents might also contribute to the disease management. Therefore, we isolated 12 bacteriophages specific to A. citrulli, from phyllosphere and rhizosphere of diseased watermelon plants. The phage strains were characterized based on their host range, plaque and virion morphology, thermal inactivation point, adsorption rate, one step growth curve, restriction fragment length polymorphism (RFLP), and genomic analysis. Transmission electron microscopy of three phage strains indicated that they belong to the order Caudovirales, family Siphoviridae. All phages lysed 30 out of 32 tested A. citrulli strains isolated in Serbia, and did not lyse other less related bacterial species. They produced clear plaques, 2 mm in diameter, on bacterial lawns of different A. citrulli strains after 24 h of incubation. The thermal inactivation point was 66 or 67°C. They were stable at pH 5–9, but were sensitive to chloroform and inactivated in either 5 or 10 min exposure to ultraviolet (UV) light. RFLP analysis using EcoRI, BsmI and BamHI enzymes did not show genetic differences among the tested phages. Adsorption rate and one step growth curve were determined for the Acidovorax phage ACF1. Draft genome sequence of the ACF1 phage was 59.377 bp in size, with guanine-cytosine (GC) content 64.5%, including 89 open reading frames. This phage shared a very high genomic identity with Acidovorax phage ACPWH, isolated in South Korea. Evaluation of systemic nature of ACF1 strain showed that it can be absorbed by roots and translocated to upper parts of watermelon plants where it survived up to 10 days.
PB  - Frontiers Media S.A.
T2  - Frontiers in Microbiology
T1  - Isolation, Characterization and Draft Genome Analysis of Bacteriophages Infecting Acidovorax citrulli
IS  - 803789
VL  - 12
DO  - 10.3389/fmicb.2021.803789
ER  - 

@article{
author = "Gašić, Katarina and Obradović, Mina and Kuzmanović, Nemanja and Zlatković, Nevena and Ivanović, Milan and Ristić, Lela and Obradović, Aleksa",
year = "2022",
abstract = "Bacterial fruit blotch and seedling blight, caused by Acidovorax citrulli, is one of the most destructive diseases of melon and watermelon in many countries. Pathogen-free seed and cultural practices are major pillars of the disease control. However, use of bacteriophages as natural biocontrol agents might also contribute to the disease management. Therefore, we isolated 12 bacteriophages specific to A. citrulli, from phyllosphere and rhizosphere of diseased watermelon plants. The phage strains were characterized based on their host range, plaque and virion morphology, thermal inactivation point, adsorption rate, one step growth curve, restriction fragment length polymorphism (RFLP), and genomic analysis. Transmission electron microscopy of three phage strains indicated that they belong to the order Caudovirales, family Siphoviridae. All phages lysed 30 out of 32 tested A. citrulli strains isolated in Serbia, and did not lyse other less related bacterial species. They produced clear plaques, 2 mm in diameter, on bacterial lawns of different A. citrulli strains after 24 h of incubation. The thermal inactivation point was 66 or 67°C. They were stable at pH 5–9, but were sensitive to chloroform and inactivated in either 5 or 10 min exposure to ultraviolet (UV) light. RFLP analysis using EcoRI, BsmI and BamHI enzymes did not show genetic differences among the tested phages. Adsorption rate and one step growth curve were determined for the Acidovorax phage ACF1. Draft genome sequence of the ACF1 phage was 59.377 bp in size, with guanine-cytosine (GC) content 64.5%, including 89 open reading frames. This phage shared a very high genomic identity with Acidovorax phage ACPWH, isolated in South Korea. Evaluation of systemic nature of ACF1 strain showed that it can be absorbed by roots and translocated to upper parts of watermelon plants where it survived up to 10 days.",
publisher = "Frontiers Media S.A.",
journal = "Frontiers in Microbiology",
title = "Isolation, Characterization and Draft Genome Analysis of Bacteriophages Infecting Acidovorax citrulli",
number = "803789",
volume = "12",
doi = "10.3389/fmicb.2021.803789"
}

Gašić, K., Obradović, M., Kuzmanović, N., Zlatković, N., Ivanović, M., Ristić, L.,& Obradović, A.. (2022). Isolation, Characterization and Draft Genome Analysis of Bacteriophages Infecting Acidovorax citrulli. in Frontiers in Microbiology
Frontiers Media S.A.., 12(803789).
https://doi.org/10.3389/fmicb.2021.803789

Gašić K, Obradović M, Kuzmanović N, Zlatković N, Ivanović M, Ristić L, Obradović A. Isolation, Characterization and Draft Genome Analysis of Bacteriophages Infecting Acidovorax citrulli. in Frontiers in Microbiology. 2022;12(803789).
doi:10.3389/fmicb.2021.803789 .

Gašić, Katarina, Obradović, Mina, Kuzmanović, Nemanja, Zlatković, Nevena, Ivanović, Milan, Ristić, Lela, Obradović, Aleksa, "Isolation, Characterization and Draft Genome Analysis of Bacteriophages Infecting Acidovorax citrulli" in Frontiers in Microbiology, 12, no. 803789 (2022),
https://doi.org/10.3389/fmicb.2021.803789 . .

TY  - JOUR
AU  - Ristić, Lela
AU  - Vučurović, Ivan
AU  - Aleksic, Goran
AU  - Nikolić, Bogdan
AU  - Đurović, Sanja
AU  - Starović, Mira
PY  - 2021
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/840
AB  - Application of three combinations of lactic acid bacteria (Lactobacillus plantarum, Lactobacillus rhamnosus), phototrophic bacteria (Rhodopseudomonas palustris) and yeast (Saccharomyces cerevisiae) with sugar cane molasses, marked as: EM1, EM5 and EM AGRO, against the phytopathogenic fungi of tomato and pepper: Fusarium oxysporum, Alternaria alternata, Botrytis cinerea, Colletotrichum sp., Verticilium dahliae and Pythium aphanidermatum was evaluated in vitro and in vivo. A combination of bacteria and yeast named EM5 showed the highest mycelium growth inhibition against B. cinerea (38.4%) in a double agar diffusion
test. In a microdilution test, the combination EM1 showed the highest inhibitory effect on B. cinerea (MIC 1x10-3 μl/ml), while EM5 showed a similar inhibitory effect towards F. oxysporum, A. alternata and Colletotrichum sp. (MIC 10 μl/ml). The use of EM1 (in concentrations 10 and 100 μl/ml) and EM AGRO (10 μl/ml) is recommended for tomato seedling protection. ЕМ1 (100 μl/ml), ЕМ5 and ЕМ AGRO (10 μl/ml) are recommended for pepper seedling protection.
PB  - Institute of Pesticides and Environmental Protection, Belgrade & Plant Protection Society of Serbia, Belgrade
T1  - Application of different combinations of lactic acid, phototrophic bacteria and yeast mixtures in control of seed and seedlings pathogens of tomato and pepper
EP  - 82
IS  - 2
SP  - 73
VL  - 36
DO  - 10.2298/PIF2102073R
ER  - 

@article{
author = "Ristić, Lela and Vučurović, Ivan and Aleksic, Goran and Nikolić, Bogdan and Đurović, Sanja and Starović, Mira",
year = "2021",
abstract = "Application of three combinations of lactic acid bacteria (Lactobacillus plantarum, Lactobacillus rhamnosus), phototrophic bacteria (Rhodopseudomonas palustris) and yeast (Saccharomyces cerevisiae) with sugar cane molasses, marked as: EM1, EM5 and EM AGRO, against the phytopathogenic fungi of tomato and pepper: Fusarium oxysporum, Alternaria alternata, Botrytis cinerea, Colletotrichum sp., Verticilium dahliae and Pythium aphanidermatum was evaluated in vitro and in vivo. A combination of bacteria and yeast named EM5 showed the highest mycelium growth inhibition against B. cinerea (38.4%) in a double agar diffusion
test. In a microdilution test, the combination EM1 showed the highest inhibitory effect on B. cinerea (MIC 1x10-3 μl/ml), while EM5 showed a similar inhibitory effect towards F. oxysporum, A. alternata and Colletotrichum sp. (MIC 10 μl/ml). The use of EM1 (in concentrations 10 and 100 μl/ml) and EM AGRO (10 μl/ml) is recommended for tomato seedling protection. ЕМ1 (100 μl/ml), ЕМ5 and ЕМ AGRO (10 μl/ml) are recommended for pepper seedling protection.",
publisher = "Institute of Pesticides and Environmental Protection, Belgrade & Plant Protection Society of Serbia, Belgrade",
title = "Application of different combinations of lactic acid, phototrophic bacteria and yeast mixtures in control of seed and seedlings pathogens of tomato and pepper",
pages = "82-73",
number = "2",
volume = "36",
doi = "10.2298/PIF2102073R"
}

Ristić, L., Vučurović, I., Aleksic, G., Nikolić, B., Đurović, S.,& Starović, M.. (2021). Application of different combinations of lactic acid, phototrophic bacteria and yeast mixtures in control of seed and seedlings pathogens of tomato and pepper. 
Institute of Pesticides and Environmental Protection, Belgrade & Plant Protection Society of Serbia, Belgrade., 36(2), 73-82.
https://doi.org/10.2298/PIF2102073R

Ristić L, Vučurović I, Aleksic G, Nikolić B, Đurović S, Starović M. Application of different combinations of lactic acid, phototrophic bacteria and yeast mixtures in control of seed and seedlings pathogens of tomato and pepper. 2021;36(2):73-82.
doi:10.2298/PIF2102073R .

Ristić, Lela, Vučurović, Ivan, Aleksic, Goran, Nikolić, Bogdan, Đurović, Sanja, Starović, Mira, "Application of different combinations of lactic acid, phototrophic bacteria and yeast mixtures in control of seed and seedlings pathogens of tomato and pepper", 36, no. 2 (2021):73-82,
https://doi.org/10.2298/PIF2102073R . .

TY  - CONF
AU  - Ristić, Lela
AU  - Vučurović, Ivan
AU  - Aleksic, Goran
AU  - Starović, Mira
PY  - 2021
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/822
PB  - Društvo za zaštitu bilja
C3  - XVI simpozijum o zaštiti bilja, Zlatibor 22. do 25. novembra, 2021  65-66
T1  - Primena smeše mlečno-kiselinskih i fototrofnih bakterija i kvasaca u suzbijanju patogena paradajza i paprike
ER  - 

@conference{
author = "Ristić, Lela and Vučurović, Ivan and Aleksic, Goran and Starović, Mira",
year = "2021",
publisher = "Društvo za zaštitu bilja",
journal = "XVI simpozijum o zaštiti bilja, Zlatibor 22. do 25. novembra, 2021  65-66",
title = "Primena smeše mlečno-kiselinskih i fototrofnih bakterija i kvasaca u suzbijanju patogena paradajza i paprike"
}

Ristić, L., Vučurović, I., Aleksic, G.,& Starović, M.. (2021). Primena smeše mlečno-kiselinskih i fototrofnih bakterija i kvasaca u suzbijanju patogena paradajza i paprike. in XVI simpozijum o zaštiti bilja, Zlatibor 22. do 25. novembra, 2021  65-66
Društvo za zaštitu bilja..

Ristić L, Vučurović I, Aleksic G, Starović M. Primena smeše mlečno-kiselinskih i fototrofnih bakterija i kvasaca u suzbijanju patogena paradajza i paprike. in XVI simpozijum o zaštiti bilja, Zlatibor 22. do 25. novembra, 2021  65-66. 2021;..

Ristić, Lela, Vučurović, Ivan, Aleksic, Goran, Starović, Mira, "Primena smeše mlečno-kiselinskih i fototrofnih bakterija i kvasaca u suzbijanju patogena paradajza i paprike" in XVI simpozijum o zaštiti bilja, Zlatibor 22. do 25. novembra, 2021  65-66 (2021).

TY  - CONF
AU  - Zivkovic, Svetlana
AU  - Ristić, Lela
AU  - Starović, Mira
AU  - Aleksic, Goran
AU  - Stošić, Stefan
PY  - 2021
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/817
PB  - Društvo za zaštitu bilja
C3  - XVI simpozijum o zaštiti bilja, Zlatibor 22. do 25. novembra 2021: 66-67
T1  - Antifungalni efekat hitosana prema Talaromyces minioluteus in vitro
ER  - 

@conference{
author = "Zivkovic, Svetlana and Ristić, Lela and Starović, Mira and Aleksic, Goran and Stošić, Stefan",
year = "2021",
publisher = "Društvo za zaštitu bilja",
journal = "XVI simpozijum o zaštiti bilja, Zlatibor 22. do 25. novembra 2021: 66-67",
title = "Antifungalni efekat hitosana prema Talaromyces minioluteus in vitro"
}

Zivkovic, S., Ristić, L., Starović, M., Aleksic, G.,& Stošić, S.. (2021). Antifungalni efekat hitosana prema Talaromyces minioluteus in vitro. in XVI simpozijum o zaštiti bilja, Zlatibor 22. do 25. novembra 2021: 66-67
Društvo za zaštitu bilja..

Zivkovic S, Ristić L, Starović M, Aleksic G, Stošić S. Antifungalni efekat hitosana prema Talaromyces minioluteus in vitro. in XVI simpozijum o zaštiti bilja, Zlatibor 22. do 25. novembra 2021: 66-67. 2021;..

Zivkovic, Svetlana, Ristić, Lela, Starović, Mira, Aleksic, Goran, Stošić, Stefan, "Antifungalni efekat hitosana prema Talaromyces minioluteus in vitro" in XVI simpozijum o zaštiti bilja, Zlatibor 22. do 25. novembra 2021: 66-67 (2021).

TY  - CONF
AU  - Aleksic, Goran
AU  - Milićević, Zoran
AU  - Zivkovic, Svetlana
AU  - Starović, Mira
AU  - Stevanović, Miloš
AU  - Ristić, Lela
AU  - Kuzmanović, Slobodan
PY  - 2021
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/807
PB  - Društvo za zaštitu bilja Srbije
C3  - Zbornik rezimea radova/XVI simpozijum o zaštiti bilja, 22-25. novembar 2021, Zlatibor: 52-53
T1  - Primena bakar citrata u suzbijanju prouzrokovača sive truleži vinove loze – Botrytis cinerea
ER  - 

@conference{
author = "Aleksic, Goran and Milićević, Zoran and Zivkovic, Svetlana and Starović, Mira and Stevanović, Miloš and Ristić, Lela and Kuzmanović, Slobodan",
year = "2021",
publisher = "Društvo za zaštitu bilja Srbije",
journal = "Zbornik rezimea radova/XVI simpozijum o zaštiti bilja, 22-25. novembar 2021, Zlatibor: 52-53",
title = "Primena bakar citrata u suzbijanju prouzrokovača sive truleži vinove loze – Botrytis cinerea"
}

Aleksic, G., Milićević, Z., Zivkovic, S., Starović, M., Stevanović, M., Ristić, L.,& Kuzmanović, S.. (2021). Primena bakar citrata u suzbijanju prouzrokovača sive truleži vinove loze – Botrytis cinerea. in Zbornik rezimea radova/XVI simpozijum o zaštiti bilja, 22-25. novembar 2021, Zlatibor: 52-53
Društvo za zaštitu bilja Srbije..

Aleksic G, Milićević Z, Zivkovic S, Starović M, Stevanović M, Ristić L, Kuzmanović S. Primena bakar citrata u suzbijanju prouzrokovača sive truleži vinove loze – Botrytis cinerea. in Zbornik rezimea radova/XVI simpozijum o zaštiti bilja, 22-25. novembar 2021, Zlatibor: 52-53. 2021;..

Aleksic, Goran, Milićević, Zoran, Zivkovic, Svetlana, Starović, Mira, Stevanović, Miloš, Ristić, Lela, Kuzmanović, Slobodan, "Primena bakar citrata u suzbijanju prouzrokovača sive truleži vinove loze – Botrytis cinerea" in Zbornik rezimea radova/XVI simpozijum o zaštiti bilja, 22-25. novembar 2021, Zlatibor: 52-53 (2021).

TY  - JOUR
AU  - Stošić, Stefan
AU  - Ristić, Lela
AU  - Savković, Željko
AU  - Ljaljevic Grbic, Milica
AU  - Vukojevic, Jelena
AU  - Živković, Svetlana
PY  - 2021
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/726
AB  - Pears are one of the oldest and the third most important fruit species grown in temperate regions. They are consumed because of their nutritional and health benefits, in fresh form or as various processed products. This article resolves the etiology of the Penicillium-like mold symptoms on pear fruits in Serbia. Samples of pear fruits with blue mold and other Penicillium-like mold symptoms were collected in Serbia from 2016 to 2019, from four storages. The recovered isolates were identified and characterized according to a polyphasic approach. Morphological and physiological analyses were performed on three media and five temperatures, respectively. Four loci (internal transcribed spacer, beta-tubulin, calmodulin, and DNA-dependent RNA polymerase II second largest subunit) were used for sequencing, genetic identification, and phylogenetic analyses. The results of the identification by conventional and molecular methods were in agreement, and they revealed that the obtained isolates belong to five species: Penicillium crustosum, P. expansum, P. italicum, Talaromyces minioluteus, and T. rugulosus. In a pathogenicity test, P. crustosum, P. expansum, T. minioluteus, and T. rugulosus produced decay on artificially inoculated pear fruits, and P. italicum induced tissue response lesions. The results of this study are the first reports of T. minioluteus and T. rugulosus as postharvest pear pathogens. Also, these are the first world records of T. minioluteus, T. rugulosus, and P. italicum on fruits of European pear. Furthermore, this is the first finding of P. crustosum, P. expansum, P. italicum, T. minioluteus, and T. rugulosus on pear fruit in Serbia.
PB  - American Phytopathological Society
T2  - Plant Disease
T1  - Penicillium and Talaromyces Species as Postharvest Pathogens of Pear Fruit (Pyrus communis) in Serbia
EP  - 3521
IS  - 11
SP  - 3510
VL  - 105
DO  - 10.1094/PDIS-01-21-0037-RE
ER  - 

@article{
author = "Stošić, Stefan and Ristić, Lela and Savković, Željko and Ljaljevic Grbic, Milica and Vukojevic, Jelena and Živković, Svetlana",
year = "2021",
abstract = "Pears are one of the oldest and the third most important fruit species grown in temperate regions. They are consumed because of their nutritional and health benefits, in fresh form or as various processed products. This article resolves the etiology of the Penicillium-like mold symptoms on pear fruits in Serbia. Samples of pear fruits with blue mold and other Penicillium-like mold symptoms were collected in Serbia from 2016 to 2019, from four storages. The recovered isolates were identified and characterized according to a polyphasic approach. Morphological and physiological analyses were performed on three media and five temperatures, respectively. Four loci (internal transcribed spacer, beta-tubulin, calmodulin, and DNA-dependent RNA polymerase II second largest subunit) were used for sequencing, genetic identification, and phylogenetic analyses. The results of the identification by conventional and molecular methods were in agreement, and they revealed that the obtained isolates belong to five species: Penicillium crustosum, P. expansum, P. italicum, Talaromyces minioluteus, and T. rugulosus. In a pathogenicity test, P. crustosum, P. expansum, T. minioluteus, and T. rugulosus produced decay on artificially inoculated pear fruits, and P. italicum induced tissue response lesions. The results of this study are the first reports of T. minioluteus and T. rugulosus as postharvest pear pathogens. Also, these are the first world records of T. minioluteus, T. rugulosus, and P. italicum on fruits of European pear. Furthermore, this is the first finding of P. crustosum, P. expansum, P. italicum, T. minioluteus, and T. rugulosus on pear fruit in Serbia.",
publisher = "American Phytopathological Society",
journal = "Plant Disease",
title = "Penicillium and Talaromyces Species as Postharvest Pathogens of Pear Fruit (Pyrus communis) in Serbia",
pages = "3521-3510",
number = "11",
volume = "105",
doi = "10.1094/PDIS-01-21-0037-RE"
}

Stošić, S., Ristić, L., Savković, Ž., Ljaljevic Grbic, M., Vukojevic, J.,& Živković, S.. (2021). Penicillium and Talaromyces Species as Postharvest Pathogens of Pear Fruit (Pyrus communis) in Serbia. in Plant Disease
American Phytopathological Society., 105(11), 3510-3521.
https://doi.org/10.1094/PDIS-01-21-0037-RE

Stošić S, Ristić L, Savković Ž, Ljaljevic Grbic M, Vukojevic J, Živković S. Penicillium and Talaromyces Species as Postharvest Pathogens of Pear Fruit (Pyrus communis) in Serbia. in Plant Disease. 2021;105(11):3510-3521.
doi:10.1094/PDIS-01-21-0037-RE .

Stošić, Stefan, Ristić, Lela, Savković, Željko, Ljaljevic Grbic, Milica, Vukojevic, Jelena, Živković, Svetlana, "Penicillium and Talaromyces Species as Postharvest Pathogens of Pear Fruit (Pyrus communis) in Serbia" in Plant Disease, 105, no. 11 (2021):3510-3521,
https://doi.org/10.1094/PDIS-01-21-0037-RE . .

TY  - JOUR
AU  - Ristić, Lela
AU  - Vučurović, Ivan
AU  - Vučurović, Ana
AU  - Živković, Svetlana
AU  - Gašić, Katarina
AU  - Kuzmanović, Slobodan
AU  - Starović, Mira
PY  - 2021
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/716
AB  - The distribution and frequency of potato leaf roll virus in the four most important potato growing regions in Serbia were studied during the seven years (2012–18). One hundred randomly collected potato tubers were sampled from each seed lot. The young leaves that developed in three weeks were sampled and tested to record infection rate. The presence of potato leaf roll virus was detected by double-antibody sandwich enzyme-linked immunosorbent assay and disease incidence was calculated using standard formula. The obtained result showed that the highest prevalence of potato leaf roll virus was detected from seed potato samples originated from the Raški region during 2018 (20.7%), while in the Moravički region, only 2.3–11.1% of the potato leaf roll virus was detected every year. The average annual potato leaf roll virus infection was the highest in 2012 (8.4%) and 2018 (8.0%). For further confirmation of potato leaf roll virus infection, reverse transcription (RT)-PCR was performed using specific primers PLRVCPvEcoRI /PLRVCPcNcoI, designed to amplify a 650 bp fragment of the full-length coat protein gene. The PCR products derived from 26 isolates were directly sequenced using the same primer pair as in RT-PCR. The coat protein sequence analysis revealed that the Serbian potato leaf roll virus isolates showed very low nucleotide diversity (95.9–100%). They shared the highest nt identities of 98.08–99.36% with the sequences of potato leaf roll virus isolates deposited in the GenBank from other parts of the world. Phylogenetic analysis and the haplotype network of the coat protein gene sequences showed that the Serbian potato leaf roll virus isolates could be classified in two different groups indicating two possible introductions of the virus to Serbia. The results of this study confirmed the importance of potato leaf roll virus in seed potato production in Serbia. Additionally, this research highlights the need for a continuous monitoring of the potato seeds produced in Serbia as well as imported seeds for the presence of potato leaf roll virus.
PB  - Springer Verlag
T2  - European Journal of Plant Pathology
T1  - Incidence and molecular characterization of potato leaf roll virus in seed potato production in Serbia
EP  - 324
IS  - 2
SP  - 315
VL  - 160
DO  - 10.1007/s10658-021-02243-6
ER  - 

@article{
author = "Ristić, Lela and Vučurović, Ivan and Vučurović, Ana and Živković, Svetlana and Gašić, Katarina and Kuzmanović, Slobodan and Starović, Mira",
year = "2021",
abstract = "The distribution and frequency of potato leaf roll virus in the four most important potato growing regions in Serbia were studied during the seven years (2012–18). One hundred randomly collected potato tubers were sampled from each seed lot. The young leaves that developed in three weeks were sampled and tested to record infection rate. The presence of potato leaf roll virus was detected by double-antibody sandwich enzyme-linked immunosorbent assay and disease incidence was calculated using standard formula. The obtained result showed that the highest prevalence of potato leaf roll virus was detected from seed potato samples originated from the Raški region during 2018 (20.7%), while in the Moravički region, only 2.3–11.1% of the potato leaf roll virus was detected every year. The average annual potato leaf roll virus infection was the highest in 2012 (8.4%) and 2018 (8.0%). For further confirmation of potato leaf roll virus infection, reverse transcription (RT)-PCR was performed using specific primers PLRVCPvEcoRI /PLRVCPcNcoI, designed to amplify a 650 bp fragment of the full-length coat protein gene. The PCR products derived from 26 isolates were directly sequenced using the same primer pair as in RT-PCR. The coat protein sequence analysis revealed that the Serbian potato leaf roll virus isolates showed very low nucleotide diversity (95.9–100%). They shared the highest nt identities of 98.08–99.36% with the sequences of potato leaf roll virus isolates deposited in the GenBank from other parts of the world. Phylogenetic analysis and the haplotype network of the coat protein gene sequences showed that the Serbian potato leaf roll virus isolates could be classified in two different groups indicating two possible introductions of the virus to Serbia. The results of this study confirmed the importance of potato leaf roll virus in seed potato production in Serbia. Additionally, this research highlights the need for a continuous monitoring of the potato seeds produced in Serbia as well as imported seeds for the presence of potato leaf roll virus.",
publisher = "Springer Verlag",
journal = "European Journal of Plant Pathology",
title = "Incidence and molecular characterization of potato leaf roll virus in seed potato production in Serbia",
pages = "324-315",
number = "2",
volume = "160",
doi = "10.1007/s10658-021-02243-6"
}

Ristić, L., Vučurović, I., Vučurović, A., Živković, S., Gašić, K., Kuzmanović, S.,& Starović, M.. (2021). Incidence and molecular characterization of potato leaf roll virus in seed potato production in Serbia. in European Journal of Plant Pathology
Springer Verlag., 160(2), 315-324.
https://doi.org/10.1007/s10658-021-02243-6

Ristić L, Vučurović I, Vučurović A, Živković S, Gašić K, Kuzmanović S, Starović M. Incidence and molecular characterization of potato leaf roll virus in seed potato production in Serbia. in European Journal of Plant Pathology. 2021;160(2):315-324.
doi:10.1007/s10658-021-02243-6 .

Ristić, Lela, Vučurović, Ivan, Vučurović, Ana, Živković, Svetlana, Gašić, Katarina, Kuzmanović, Slobodan, Starović, Mira, "Incidence and molecular characterization of potato leaf roll virus in seed potato production in Serbia" in European Journal of Plant Pathology, 160, no. 2 (2021):315-324,
https://doi.org/10.1007/s10658-021-02243-6 . .

TY  - JOUR
AU  - Stanković, Ivana
AU  - Vučurović, Ana
AU  - Zečević, Katarina
AU  - Petrović, Branka
AU  - Ristić, Lela
AU  - Vučurović, Ivan
AU  - Krstić, Branka
PY  - 2021
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/643
AB  - Aim of study: To report the occurrence of Pepino mosaic virus (PepMV) on tomato in Serbia and to genetically characterize Serbian PepMV isolates. Area of study: Tomato samples showing virus-like symptoms were collected in the Bogojevce locality (Jablanica District, Serbia). Material and methods: Collected tomato samples were assayed by DAS-ELISA using antisera against eight economically important or quarantine tomato viruses. Three selected isolates of naturally infected tomato plants were mechanically transmitted to tomato ‘Novosadski jabučar’ seedlings. For confirmation of PepMV infection, RT-PCR was performed using specific primers PepMV TGB F/PepMV UTR R. Maximum-likelihood phylogenetic tree was constructed with 47 complete CP gene sequences of PepMV to determine the genetic relationship of Serbian PepMV isolates with those from other parts of the world. Main results: The results of DAS-ELISA indicated the presence of PepMV in all tested samples. Mechanically inoculated ‘Novosadski jabučar’ seedlings expressed yellow spots and light and dark green patches, bubbling, and curled leaves. All tested tomato plants were RTPCR positive for the presence of PepMV. The CP sequence analysis revealed that the Serbian PepMV isolates were completely identical among themselves and shared the highest nucleotide identity of 95.1% (99.2% aa identity) with isolate from Spain (FJ263341). Phylogenetic analysis showed clustering of the Serbian PepMV isolates into CH2 strain, but they formed separate subgroup within CH2 strain. Research highlights: This is the first data of the presence of PepMV in protected tomato production in Serbia. Considering increased incidence and rapid spread in Europe, the presence of PepMV on tomato could therefore represent serious threat to this valuable crop in Serbia
PB  - Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA)
T2  - Spanish Journal of Agricultural Research
T1  - Short communication: Pepino mosaic virus, a new threat for Serbia’s tomatoes
IS  - 4
VL  - 18
DO  - 10.5424/sjar/2020184-16244
ER  - 

@article{
author = "Stanković, Ivana and Vučurović, Ana and Zečević, Katarina and Petrović, Branka and Ristić, Lela and Vučurović, Ivan and Krstić, Branka",
year = "2021",
abstract = "Aim of study: To report the occurrence of Pepino mosaic virus (PepMV) on tomato in Serbia and to genetically characterize Serbian PepMV isolates. Area of study: Tomato samples showing virus-like symptoms were collected in the Bogojevce locality (Jablanica District, Serbia). Material and methods: Collected tomato samples were assayed by DAS-ELISA using antisera against eight economically important or quarantine tomato viruses. Three selected isolates of naturally infected tomato plants were mechanically transmitted to tomato ‘Novosadski jabučar’ seedlings. For confirmation of PepMV infection, RT-PCR was performed using specific primers PepMV TGB F/PepMV UTR R. Maximum-likelihood phylogenetic tree was constructed with 47 complete CP gene sequences of PepMV to determine the genetic relationship of Serbian PepMV isolates with those from other parts of the world. Main results: The results of DAS-ELISA indicated the presence of PepMV in all tested samples. Mechanically inoculated ‘Novosadski jabučar’ seedlings expressed yellow spots and light and dark green patches, bubbling, and curled leaves. All tested tomato plants were RTPCR positive for the presence of PepMV. The CP sequence analysis revealed that the Serbian PepMV isolates were completely identical among themselves and shared the highest nucleotide identity of 95.1% (99.2% aa identity) with isolate from Spain (FJ263341). Phylogenetic analysis showed clustering of the Serbian PepMV isolates into CH2 strain, but they formed separate subgroup within CH2 strain. Research highlights: This is the first data of the presence of PepMV in protected tomato production in Serbia. Considering increased incidence and rapid spread in Europe, the presence of PepMV on tomato could therefore represent serious threat to this valuable crop in Serbia",
publisher = "Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA)",
journal = "Spanish Journal of Agricultural Research",
title = "Short communication: Pepino mosaic virus, a new threat for Serbia’s tomatoes",
number = "4",
volume = "18",
doi = "10.5424/sjar/2020184-16244"
}

Stanković, I., Vučurović, A., Zečević, K., Petrović, B., Ristić, L., Vučurović, I.,& Krstić, B.. (2021). Short communication: Pepino mosaic virus, a new threat for Serbia’s tomatoes. in Spanish Journal of Agricultural Research
Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA)., 18(4).
https://doi.org/10.5424/sjar/2020184-16244

Stanković I, Vučurović A, Zečević K, Petrović B, Ristić L, Vučurović I, Krstić B. Short communication: Pepino mosaic virus, a new threat for Serbia’s tomatoes. in Spanish Journal of Agricultural Research. 2021;18(4).
doi:10.5424/sjar/2020184-16244 .

Stanković, Ivana, Vučurović, Ana, Zečević, Katarina, Petrović, Branka, Ristić, Lela, Vučurović, Ivan, Krstić, Branka, "Short communication: Pepino mosaic virus, a new threat for Serbia’s tomatoes" in Spanish Journal of Agricultural Research, 18, no. 4 (2021),
https://doi.org/10.5424/sjar/2020184-16244 . .

Zivkovic, S., Ristić, D.,& Stošić, S.. (2021). First report of Penicillium olsonii causing postharvest fruit rot on tomato in Serbia. in Plant Disease
American Phytopathological Society., 105(8), 2246-2246.
https://doi.org/10.1094/PDIS-02-21-0323-PDN

Zivkovic S, Ristić D, Stošić S. First report of Penicillium olsonii causing postharvest fruit rot on tomato in Serbia. in Plant Disease. 2021;105(8):2246-2246.
doi:10.1094/PDIS-02-21-0323-PDN .

Zivkovic, Svetlana, Ristić, Danijela, Stošić, Stefan, "First report of Penicillium olsonii causing postharvest fruit rot on tomato in Serbia" in Plant Disease, 105, no. 8 (2021):2246-2246,
https://doi.org/10.1094/PDIS-02-21-0323-PDN . .

TY  - JOUR
AU  - Stošić, Stefan
AU  - Ristić, Danijela
AU  - Živković, Svetlana
PY  - 2021
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/620
AB  - Mandarin fruits are one of the most popular among the Citrus genus. They are consumed because of their nutritional and health benefits, as well as pleasant taste and smell. This paper describes the identification and characterization of Penicillium expansum, isolated from molded mandarin fruits. The obtained isolates were cultivated on five media [Czapek Yeast Autolysate agar (CYA), MEA (Malt extract agar), Creatine sucrose agar (CREA), Yeast extract sucrose agar (YES), and Oatmeal agar (OA)] and at five different incubation temperatures (5, 15, 25, 30, and 37 °C). Isolates were sequenced for two molecular loci: internal transcribed spacer and beta-tubulin. Based on the results from morphological, physiological, molecular, and phylogenetic analyses, the recovered isolates were identified as P. expansum. The isolated species was confirmed as pathogenic to mandarin fruits in a pathogenicity test. To the best of our knowledge, this is the first report of P. expansum as a postharvest pathogen of mandarin fruit in Serbia.
PB  - Matica Srpska
T2  - Zbornik Matice srpske za prirodne nauke
T1  - Postharvest decay of mandarin fruit in Serbia caused by Penicillium expansum
EP  - 44
SP  - 29
VL  - 140
DO  - 10.2298/ZMSPN2140029S
ER  - 

@article{
author = "Stošić, Stefan and Ristić, Danijela and Živković, Svetlana",
year = "2021",
abstract = "Mandarin fruits are one of the most popular among the Citrus genus. They are consumed because of their nutritional and health benefits, as well as pleasant taste and smell. This paper describes the identification and characterization of Penicillium expansum, isolated from molded mandarin fruits. The obtained isolates were cultivated on five media [Czapek Yeast Autolysate agar (CYA), MEA (Malt extract agar), Creatine sucrose agar (CREA), Yeast extract sucrose agar (YES), and Oatmeal agar (OA)] and at five different incubation temperatures (5, 15, 25, 30, and 37 °C). Isolates were sequenced for two molecular loci: internal transcribed spacer and beta-tubulin. Based on the results from morphological, physiological, molecular, and phylogenetic analyses, the recovered isolates were identified as P. expansum. The isolated species was confirmed as pathogenic to mandarin fruits in a pathogenicity test. To the best of our knowledge, this is the first report of P. expansum as a postharvest pathogen of mandarin fruit in Serbia.",
publisher = "Matica Srpska",
journal = "Zbornik Matice srpske za prirodne nauke",
title = "Postharvest decay of mandarin fruit in Serbia caused by Penicillium expansum",
pages = "44-29",
volume = "140",
doi = "10.2298/ZMSPN2140029S"
}

Stošić, S., Ristić, D.,& Živković, S.. (2021). Postharvest decay of mandarin fruit in Serbia caused by Penicillium expansum. in Zbornik Matice srpske za prirodne nauke
Matica Srpska., 140, 29-44.
https://doi.org/10.2298/ZMSPN2140029S

Stošić S, Ristić D, Živković S. Postharvest decay of mandarin fruit in Serbia caused by Penicillium expansum. in Zbornik Matice srpske za prirodne nauke. 2021;140:29-44.
doi:10.2298/ZMSPN2140029S .

Stošić, Stefan, Ristić, Danijela, Živković, Svetlana, "Postharvest decay of mandarin fruit in Serbia caused by Penicillium expansum" in Zbornik Matice srpske za prirodne nauke, 140 (2021):29-44,
https://doi.org/10.2298/ZMSPN2140029S . .

TY  - CONF
AU  - Banović Đeri, Bojana
AU  - Vidanović, Dejan
AU  - Tešović, Bojana
AU  - Petrović, Tamaš
AU  - Ristić, Danijela
AU  - Vučurović, Ivan
AU  - Dudić, Dragana
PY  - 2021
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/978
AB  - Birbica, Faculty of Sciences, Departmologia Seent of Biology and Ecology

Positively oriented single stranded RNA viruses [ssRNA(+)] persistently affect health and well-being of all eukaryotes, including plants, animals and humans (i.e. SARS-CoV-2, yellow fever, hepatitis C, zika, West Nile, pepper mild mottle virus, etc.). How come these viruses are so wide spread and hard to eradicate? Besides their high changeability, another major reason is their ability to mimic host processes upon entering the host. Only recently it was revealed that ssRNA(+) viruses undergo methylation inside the host in the process that is similar to the methylation of the hosts’ own mRNAs. Such process may enable or disable virus to avoid some of the host’s defense mechanisms, but it inevitably impacts viral stability and fitness. Studies on this topic have only started, opening even more questions, with major ones being: how ssRNA(+) methylation, that occurs in the host, impacts viral pathogenicity and are these methylation patterns different in different hosts and for different ssRNA(+) viruses or do these viral methylomes share more universal pattern in concordance with their similar genome organization? Among numerous different methylation patterns of RNA, this research focused on N6-methyladenosine (m6A), as the most common and abundant methylation in eukaryotes, which was confirmed to be present in ssRNA(+) viruses as well. This study searched for patterns in the primary sequences and secondary structures of ssRNA(+) that are associated to m6A methylation sites relying on the experimentally obtained m6A datasets for eukaryotes and eukaryotic ssRNA(+) viruses. The results are discussed in view of datasets characteristics and study approach.
PB  - Novi Sad : University of Novi Sad, Faculty of Sciences, Department of Biology and Ecology
C3  - Biologia Serbica, Book of Abstracts, Belgrade BioInformatics Conference 2021 21-25 June 2021, Vinča, Serbia
T1  - Bioinformatics analysis of eukaryotic positively oriented single stranded RNA viruses
SP  - 29
ER  - 

@conference{
author = "Banović Đeri, Bojana and Vidanović, Dejan and Tešović, Bojana and Petrović, Tamaš and Ristić, Danijela and Vučurović, Ivan and Dudić, Dragana",
year = "2021",
abstract = "Birbica, Faculty of Sciences, Departmologia Seent of Biology and Ecology

Positively oriented single stranded RNA viruses [ssRNA(+)] persistently affect health and well-being of all eukaryotes, including plants, animals and humans (i.e. SARS-CoV-2, yellow fever, hepatitis C, zika, West Nile, pepper mild mottle virus, etc.). How come these viruses are so wide spread and hard to eradicate? Besides their high changeability, another major reason is their ability to mimic host processes upon entering the host. Only recently it was revealed that ssRNA(+) viruses undergo methylation inside the host in the process that is similar to the methylation of the hosts’ own mRNAs. Such process may enable or disable virus to avoid some of the host’s defense mechanisms, but it inevitably impacts viral stability and fitness. Studies on this topic have only started, opening even more questions, with major ones being: how ssRNA(+) methylation, that occurs in the host, impacts viral pathogenicity and are these methylation patterns different in different hosts and for different ssRNA(+) viruses or do these viral methylomes share more universal pattern in concordance with their similar genome organization? Among numerous different methylation patterns of RNA, this research focused on N6-methyladenosine (m6A), as the most common and abundant methylation in eukaryotes, which was confirmed to be present in ssRNA(+) viruses as well. This study searched for patterns in the primary sequences and secondary structures of ssRNA(+) that are associated to m6A methylation sites relying on the experimentally obtained m6A datasets for eukaryotes and eukaryotic ssRNA(+) viruses. The results are discussed in view of datasets characteristics and study approach.",
publisher = "Novi Sad : University of Novi Sad, Faculty of Sciences, Department of Biology and Ecology",
journal = "Biologia Serbica, Book of Abstracts, Belgrade BioInformatics Conference 2021 21-25 June 2021, Vinča, Serbia",
title = "Bioinformatics analysis of eukaryotic positively oriented single stranded RNA viruses",
pages = "29"
}

Banović Đeri, B., Vidanović, D., Tešović, B., Petrović, T., Ristić, D., Vučurović, I.,& Dudić, D.. (2021). Bioinformatics analysis of eukaryotic positively oriented single stranded RNA viruses. in Biologia Serbica, Book of Abstracts, Belgrade BioInformatics Conference 2021 21-25 June 2021, Vinča, Serbia
Novi Sad : University of Novi Sad, Faculty of Sciences, Department of Biology and Ecology., 29.

Banović Đeri B, Vidanović D, Tešović B, Petrović T, Ristić D, Vučurović I, Dudić D. Bioinformatics analysis of eukaryotic positively oriented single stranded RNA viruses. in Biologia Serbica, Book of Abstracts, Belgrade BioInformatics Conference 2021 21-25 June 2021, Vinča, Serbia. 2021;:29..

Banović Đeri, Bojana, Vidanović, Dejan, Tešović, Bojana, Petrović, Tamaš, Ristić, Danijela, Vučurović, Ivan, Dudić, Dragana, "Bioinformatics analysis of eukaryotic positively oriented single stranded RNA viruses" in Biologia Serbica, Book of Abstracts, Belgrade BioInformatics Conference 2021 21-25 June 2021, Vinča, Serbia (2021):29.

TY  - JOUR
AU  - Starović, Mira
AU  - Ristić, Danijela
AU  - Pavlović, Snežana
AU  - MÖZCAN, Mehmet
AU  - Jošić, Dragana
PY  - 2021
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/619
AB  - Leaf spot disease caused by Cercospora beticola Sacc. is the most destructive foliar disease of beet. Cercospora leaf spot is controlled primarily by fungicides because the non-chemical alternatives do not provide commercially viable control. One of the ways of reducing chemical application is the use of different essential oils (EOs) or antagonistic plant growth-promoting rhizobacteria (PGPB). This study evaluates several EOs and PGPB belonging to Pseudomonas chlororaphis as possible control agents of this pathogen. Antifungal properties were determined by in vitro microdilution method against five C. beticola monosporial isolates originated from the locality Brus, Serbia (53°53’ N, 21°04’E and 429 m above sea level) using EOs from medicinal plants: Turkish pickling herb (Echinophora tenuifolia), oregano (Origanum vulgare), basil (Ocimum basilicum), and myrtle (Myrtus communis) obtained by a hydro-distillation method. All tested oils displayed some antifungal activity against the fungal isolates. Origanum vulgare EO demonstrated the strongest antifungal activity (MIC - 0.0055±0.0051mg/mL), Ocimum basilicum slightly lower (MIC - 0.075±0.045mg/mL), followed by Myrtus communis (MIC - 0.775±0.045 mg/ mL) and Echinophora tenuifolia (MIC - 7.75±4.5 mg/mL). Five tested P. chlororaphis strains exhibited some antagonistic effect against C. beticola. Overnight culture (ONC) of P. chlororaphis strain E65 induced the highest percentage of inhibition (75.8%), followed by N3 (72.0%). A cell-free supernatant (CFS) and the CFS treated with EDTA (CFS-EDTA) of these strains showed similar inhibition of 60.2 and 56.0%, and both strains suppressed C. beticola growth. P. chlororaphis strains M1 and K113 also reduced the fungal growth by 67-70% using ONC and between 48-57% using different CFS fractions. The strains L1 and B25 caused inhibition of 60% using ONC and 50% by CFS. The lowest inhibition (~40%) by CFS-EDTA and heat-treated cell-free supernatant (HT-CFS) was recorded for B25, which was used as a reference strain. The tested isolates of C. beticola were susceptible to all selected essential oils and P. chlororaphis strains E25, N3, M1, and K113 in vitro, making them a promising non-chemical control agent. It is recommended that these findings should be tested in field conditions.
PB  - Matica Srpska
T2  - Zbornik Matice srpske za prirodne nauke
T1  - Antifungal activity of plant essential oils and Pseudomonas chlororaphis strains against Cercospora beticola Sacc.
EP  - 19
SP  - 9
VL  - 140
DO  - 10.2298/ZMSPN2140009S
ER  - 

@article{
author = "Starović, Mira and Ristić, Danijela and Pavlović, Snežana and MÖZCAN, Mehmet and Jošić, Dragana",
year = "2021",
abstract = "Leaf spot disease caused by Cercospora beticola Sacc. is the most destructive foliar disease of beet. Cercospora leaf spot is controlled primarily by fungicides because the non-chemical alternatives do not provide commercially viable control. One of the ways of reducing chemical application is the use of different essential oils (EOs) or antagonistic plant growth-promoting rhizobacteria (PGPB). This study evaluates several EOs and PGPB belonging to Pseudomonas chlororaphis as possible control agents of this pathogen. Antifungal properties were determined by in vitro microdilution method against five C. beticola monosporial isolates originated from the locality Brus, Serbia (53°53’ N, 21°04’E and 429 m above sea level) using EOs from medicinal plants: Turkish pickling herb (Echinophora tenuifolia), oregano (Origanum vulgare), basil (Ocimum basilicum), and myrtle (Myrtus communis) obtained by a hydro-distillation method. All tested oils displayed some antifungal activity against the fungal isolates. Origanum vulgare EO demonstrated the strongest antifungal activity (MIC - 0.0055±0.0051mg/mL), Ocimum basilicum slightly lower (MIC - 0.075±0.045mg/mL), followed by Myrtus communis (MIC - 0.775±0.045 mg/ mL) and Echinophora tenuifolia (MIC - 7.75±4.5 mg/mL). Five tested P. chlororaphis strains exhibited some antagonistic effect against C. beticola. Overnight culture (ONC) of P. chlororaphis strain E65 induced the highest percentage of inhibition (75.8%), followed by N3 (72.0%). A cell-free supernatant (CFS) and the CFS treated with EDTA (CFS-EDTA) of these strains showed similar inhibition of 60.2 and 56.0%, and both strains suppressed C. beticola growth. P. chlororaphis strains M1 and K113 also reduced the fungal growth by 67-70% using ONC and between 48-57% using different CFS fractions. The strains L1 and B25 caused inhibition of 60% using ONC and 50% by CFS. The lowest inhibition (~40%) by CFS-EDTA and heat-treated cell-free supernatant (HT-CFS) was recorded for B25, which was used as a reference strain. The tested isolates of C. beticola were susceptible to all selected essential oils and P. chlororaphis strains E25, N3, M1, and K113 in vitro, making them a promising non-chemical control agent. It is recommended that these findings should be tested in field conditions.",
publisher = "Matica Srpska",
journal = "Zbornik Matice srpske za prirodne nauke",
title = "Antifungal activity of plant essential oils and Pseudomonas chlororaphis strains against Cercospora beticola Sacc.",
pages = "19-9",
volume = "140",
doi = "10.2298/ZMSPN2140009S"
}

Starović, M., Ristić, D., Pavlović, S., MÖZCAN, M.,& Jošić, D.. (2021). Antifungal activity of plant essential oils and Pseudomonas chlororaphis strains against Cercospora beticola Sacc.. in Zbornik Matice srpske za prirodne nauke
Matica Srpska., 140, 9-19.
https://doi.org/10.2298/ZMSPN2140009S

Starović M, Ristić D, Pavlović S, MÖZCAN M, Jošić D. Antifungal activity of plant essential oils and Pseudomonas chlororaphis strains against Cercospora beticola Sacc.. in Zbornik Matice srpske za prirodne nauke. 2021;140:9-19.
doi:10.2298/ZMSPN2140009S .

Starović, Mira, Ristić, Danijela, Pavlović, Snežana, MÖZCAN, Mehmet, Jošić, Dragana, "Antifungal activity of plant essential oils and Pseudomonas chlororaphis strains against Cercospora beticola Sacc." in Zbornik Matice srpske za prirodne nauke, 140 (2021):9-19,
https://doi.org/10.2298/ZMSPN2140009S . .

TY  - JOUR
AU  - Stošić, Stefan
AU  - Ristić, Lela
AU  - Gašić, Katarina
AU  - Starović, Mira
AU  - Ljaljevic Grbic, Milica
AU  - Vukojevic, Jelena
AU  - Živković, Svetlana
PY  - 2020
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/717
AB  - Talaromyces minioluteus is one of the important species of genus Talaromyces, which has cosmopolitan distribution and is encountered on a wide range of different habitats. This species has not been considered as an important plant pathogen, even though it has been isolated from various plant hosts. Fruits and vegetables with Penicillium-like mold symptoms were collected from 2015 to 2017 from markets in Serbia. Isolates originating from quince, tomato, and orange fruits, onion bulbs, and potato tubers were identified and characterized on a morphological, physiological, and molecular level. Morphological and physiological examination included observing micromorphology, testing growth on six different media and at five different temperatures, and production of three enzymes. Molecular identification and characterization were performed using four molecular markers: internal transcribed spacer, β-tubulin, calmodulin, and DNA-dependent RNA polymerase II second largest subunit. The results of morphological and molecular analyses were in agreement, and they proved that the obtained isolates are T. minioluteus. In the pathogenicity assay, T. minioluteus was confirmed as a pathogen of all species tested with the exception of potato tubers. This is the first report of T. minioluteus as a postharvest plant pathogen on quince, tomato, and orange fruit and onion bulbs. Also, this is the first record of T. minioluteus in Serbia.
PB  - The American Phytopathological Society
T2  - Plant Disease
T1  - Talaromyces minioluteus: New Postharvest Fungal Pathogen in Serbia
EP  - 667
IS  - 3
SP  - 656
VL  - 104
DO  - 10.1094/PDIS-08-19-1806-RE
ER  - 

@article{
author = "Stošić, Stefan and Ristić, Lela and Gašić, Katarina and Starović, Mira and Ljaljevic Grbic, Milica and Vukojevic, Jelena and Živković, Svetlana",
year = "2020",
abstract = "Talaromyces minioluteus is one of the important species of genus Talaromyces, which has cosmopolitan distribution and is encountered on a wide range of different habitats. This species has not been considered as an important plant pathogen, even though it has been isolated from various plant hosts. Fruits and vegetables with Penicillium-like mold symptoms were collected from 2015 to 2017 from markets in Serbia. Isolates originating from quince, tomato, and orange fruits, onion bulbs, and potato tubers were identified and characterized on a morphological, physiological, and molecular level. Morphological and physiological examination included observing micromorphology, testing growth on six different media and at five different temperatures, and production of three enzymes. Molecular identification and characterization were performed using four molecular markers: internal transcribed spacer, β-tubulin, calmodulin, and DNA-dependent RNA polymerase II second largest subunit. The results of morphological and molecular analyses were in agreement, and they proved that the obtained isolates are T. minioluteus. In the pathogenicity assay, T. minioluteus was confirmed as a pathogen of all species tested with the exception of potato tubers. This is the first report of T. minioluteus as a postharvest plant pathogen on quince, tomato, and orange fruit and onion bulbs. Also, this is the first record of T. minioluteus in Serbia.",
publisher = "The American Phytopathological Society",
journal = "Plant Disease",
title = "Talaromyces minioluteus: New Postharvest Fungal Pathogen in Serbia",
pages = "667-656",
number = "3",
volume = "104",
doi = "10.1094/PDIS-08-19-1806-RE"
}

Stošić, S., Ristić, L., Gašić, K., Starović, M., Ljaljevic Grbic, M., Vukojevic, J.,& Živković, S.. (2020). Talaromyces minioluteus: New Postharvest Fungal Pathogen in Serbia. in Plant Disease
The American Phytopathological Society., 104(3), 656-667.
https://doi.org/10.1094/PDIS-08-19-1806-RE

Stošić S, Ristić L, Gašić K, Starović M, Ljaljevic Grbic M, Vukojevic J, Živković S. Talaromyces minioluteus: New Postharvest Fungal Pathogen in Serbia. in Plant Disease. 2020;104(3):656-667.
doi:10.1094/PDIS-08-19-1806-RE .

Stošić, Stefan, Ristić, Lela, Gašić, Katarina, Starović, Mira, Ljaljevic Grbic, Milica, Vukojevic, Jelena, Živković, Svetlana, "Talaromyces minioluteus: New Postharvest Fungal Pathogen in Serbia" in Plant Disease, 104, no. 3 (2020):656-667,
https://doi.org/10.1094/PDIS-08-19-1806-RE . .

TY  - JOUR
AU  - Stanković, Ivana
AU  - Vučurović, Ana
AU  - Zečević, Katarina
AU  - Petrović, Branka
AU  - Ristić, Danijela
AU  - Vucurović, Ivan
AU  - Krstić, Branka
PY  - 2020
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/594
AB  - A subset of ornamentals grown in Serbia were surveyed for the presence of impatiens necrotic spot tospovirus (INSV) from 2008 to 2018. Out of 1578 samples tested for the presence of the virus using double-antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA), INSV was detected in 26 Begonia sp. and four Tulipa sp. samples collected in 2008 and in 10 samples of each of Begonia sp., Impatiens walleriana and I. hawkeri 'SunPatiens' and 'New Guinea' collected in 2018. INSV was not detected in any samples collected during 2009-2017. For further confirmation, the nucleotide sequence of the INSV nucleocapsid (N) gene was obtained by reverse transcription polymerase chain reaction (RT-PCR) in two and four samples collected in 2008 and 2018, respectively. The success of mechanical transmission of INSV isolates from different hosts was variable depending on isolates. Serbian INSV isolates showed very low nucleotide diversity and they were closely related to the Chinese and Japanese isolates already reported in the literature, indicating that the Serbian isolates might be of Asian origin. Phylogenetic analysis and the median-joining haplotype network of the N gene sequences showed clustering of Serbian INSV isolates in the Asian clade into two different subclades with different recent common ancestors implying two independent introductions. The sporadic occurrence of INSV with high temporal distance is a likely consequence of virus spread through the international trade in ornamental planting material. Therefore, the establishment of the virus should be considered and prevented when introducing new plant material into the country. This is also the first report of the tulip as a natural host of INSV.
PB  - Springer, New York
T2  - Journal of Plant Pathology
T1  - Occurrence and molecular characterization of Impatiens necrotic spot tospovirus in ornamentals in Serbia
EP  - 797
IS  - 3
SP  - 787
VL  - 102
DO  - 10.1007/s42161-020-00504-7
ER  - 

@article{
author = "Stanković, Ivana and Vučurović, Ana and Zečević, Katarina and Petrović, Branka and Ristić, Danijela and Vucurović, Ivan and Krstić, Branka",
year = "2020",
abstract = "A subset of ornamentals grown in Serbia were surveyed for the presence of impatiens necrotic spot tospovirus (INSV) from 2008 to 2018. Out of 1578 samples tested for the presence of the virus using double-antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA), INSV was detected in 26 Begonia sp. and four Tulipa sp. samples collected in 2008 and in 10 samples of each of Begonia sp., Impatiens walleriana and I. hawkeri 'SunPatiens' and 'New Guinea' collected in 2018. INSV was not detected in any samples collected during 2009-2017. For further confirmation, the nucleotide sequence of the INSV nucleocapsid (N) gene was obtained by reverse transcription polymerase chain reaction (RT-PCR) in two and four samples collected in 2008 and 2018, respectively. The success of mechanical transmission of INSV isolates from different hosts was variable depending on isolates. Serbian INSV isolates showed very low nucleotide diversity and they were closely related to the Chinese and Japanese isolates already reported in the literature, indicating that the Serbian isolates might be of Asian origin. Phylogenetic analysis and the median-joining haplotype network of the N gene sequences showed clustering of Serbian INSV isolates in the Asian clade into two different subclades with different recent common ancestors implying two independent introductions. The sporadic occurrence of INSV with high temporal distance is a likely consequence of virus spread through the international trade in ornamental planting material. Therefore, the establishment of the virus should be considered and prevented when introducing new plant material into the country. This is also the first report of the tulip as a natural host of INSV.",
publisher = "Springer, New York",
journal = "Journal of Plant Pathology",
title = "Occurrence and molecular characterization of Impatiens necrotic spot tospovirus in ornamentals in Serbia",
pages = "797-787",
number = "3",
volume = "102",
doi = "10.1007/s42161-020-00504-7"
}

Stanković, I., Vučurović, A., Zečević, K., Petrović, B., Ristić, D., Vucurović, I.,& Krstić, B.. (2020). Occurrence and molecular characterization of Impatiens necrotic spot tospovirus in ornamentals in Serbia. in Journal of Plant Pathology
Springer, New York., 102(3), 787-797.
https://doi.org/10.1007/s42161-020-00504-7

Stanković I, Vučurović A, Zečević K, Petrović B, Ristić D, Vucurović I, Krstić B. Occurrence and molecular characterization of Impatiens necrotic spot tospovirus in ornamentals in Serbia. in Journal of Plant Pathology. 2020;102(3):787-797.
doi:10.1007/s42161-020-00504-7 .

Stanković, Ivana, Vučurović, Ana, Zečević, Katarina, Petrović, Branka, Ristić, Danijela, Vucurović, Ivan, Krstić, Branka, "Occurrence and molecular characterization of Impatiens necrotic spot tospovirus in ornamentals in Serbia" in Journal of Plant Pathology, 102, no. 3 (2020):787-797,
https://doi.org/10.1007/s42161-020-00504-7 . .

TY  - JOUR
AU  - Zivkovic, Svetlana
AU  - Stošić, Stefan
AU  - Ristić, Danijela
AU  - Vučurović, Ivan
AU  - Stevanović, Miloš
PY  - 2019
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/917
AB  - Lactobacillus plantarum, one of the most widespread lactic acid bacte¬ria, exert a strong antagonistic activity against many microorganisms. The present study was conducted to determine in vitro and in situ antagonistic potential of L. plantarum (DSM 20174) for control postharvest decay caused by phytopathogenic fungi: Aspergillus flavus, Colletotrichum acutatum, Colletotrichum gloeosporioides, and Fusarium avenaceum. The results obtained in in vitro assays showed that L. plantarum had a stronger inhibitory effect on spore germination than on mycelia growth of all tested fungi. After 3 days of incubation, the diameter of inhibition zones ranged from 11.67 mm for C. gloeosporioides to 14.67 mm for C. acutatum. The bacterial suspension of L. plantarum significantly inhibited conidial germination of all postharvest pathogens (89.62-97.61%). In situ assays showed that treatment with L. plantarum efficiently inhibited necrosis ranging from 42.54% for C. acutatum to 54.47% for A. flavus. The disease incidence in L. plantarum treated fruits was statistically significantly lower than in the positive control for all fungi tested (P<0.05). The presented data demonstrate the antagonistic potential of L. plantarum (DSM 20174) and indicate the possibility of using this bacterial strain as a biological agent to control postharvest fungal pathogens.
PB  - Matica srpska
T2  - Zbornik Matice srpske za prirodne nauke
T1  - Antagonistic potential of lactobacillus plantarum against some postharvest pathogenic fungi
EP  - 88
SP  - 79
VL  - 136
DO  - 10.2298/zmspn1936079z
ER  - 

@article{
author = "Zivkovic, Svetlana and Stošić, Stefan and Ristić, Danijela and Vučurović, Ivan and Stevanović, Miloš",
year = "2019",
abstract = "Lactobacillus plantarum, one of the most widespread lactic acid bacte¬ria, exert a strong antagonistic activity against many microorganisms. The present study was conducted to determine in vitro and in situ antagonistic potential of L. plantarum (DSM 20174) for control postharvest decay caused by phytopathogenic fungi: Aspergillus flavus, Colletotrichum acutatum, Colletotrichum gloeosporioides, and Fusarium avenaceum. The results obtained in in vitro assays showed that L. plantarum had a stronger inhibitory effect on spore germination than on mycelia growth of all tested fungi. After 3 days of incubation, the diameter of inhibition zones ranged from 11.67 mm for C. gloeosporioides to 14.67 mm for C. acutatum. The bacterial suspension of L. plantarum significantly inhibited conidial germination of all postharvest pathogens (89.62-97.61%). In situ assays showed that treatment with L. plantarum efficiently inhibited necrosis ranging from 42.54% for C. acutatum to 54.47% for A. flavus. The disease incidence in L. plantarum treated fruits was statistically significantly lower than in the positive control for all fungi tested (P<0.05). The presented data demonstrate the antagonistic potential of L. plantarum (DSM 20174) and indicate the possibility of using this bacterial strain as a biological agent to control postharvest fungal pathogens.",
publisher = "Matica srpska",
journal = "Zbornik Matice srpske za prirodne nauke",
title = "Antagonistic potential of lactobacillus plantarum against some postharvest pathogenic fungi",
pages = "88-79",
volume = "136",
doi = "10.2298/zmspn1936079z"
}

Zivkovic, S., Stošić, S., Ristić, D., Vučurović, I.,& Stevanović, M.. (2019). Antagonistic potential of lactobacillus plantarum against some postharvest pathogenic fungi. in Zbornik Matice srpske za prirodne nauke
Matica srpska., 136, 79-88.
https://doi.org/10.2298/zmspn1936079z

Zivkovic S, Stošić S, Ristić D, Vučurović I, Stevanović M. Antagonistic potential of lactobacillus plantarum against some postharvest pathogenic fungi. in Zbornik Matice srpske za prirodne nauke. 2019;136:79-88.
doi:10.2298/zmspn1936079z .

Zivkovic, Svetlana, Stošić, Stefan, Ristić, Danijela, Vučurović, Ivan, Stevanović, Miloš, "Antagonistic potential of lactobacillus plantarum against some postharvest pathogenic fungi" in Zbornik Matice srpske za prirodne nauke, 136 (2019):79-88,
https://doi.org/10.2298/zmspn1936079z . .

TY  - JOUR
AU  - Ristić, Danijela
AU  - Vučurović, Ivan
AU  - Kuzmanović, Slobodan
AU  - Pfaf-Dolovac, Erika
AU  - Aleksić, Goran
AU  - Vučurović, Ana
AU  - Starović, Mira
PY  - 2019
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/560
AB  - It is essential that certified potatoes are free from known viruses which can negatively affect quality and yield. However, very little is known about the distribution and frequency of Potato virus S (PVS) in Serbia. Until 2014, PVS was present sporadically in the domestic seed potato production. The incidence of PVS was studied by a molecular method over 3 years (2014-2016) in four important potato-growing regions (Moravidd. Zlatiborski, Raski and Macvanski) and in different cultivars. The results showed that the incidence of PVS increased steadily over 3 years from 1.52 to 8.84%. The Moravicki region had the highest incidence (13.06%) and Desiree was the most susceptible cultivar with a mean PVS incidence of 8.2% followed by Marabel and Riviera. The highest significant statistical difference was between the cultivars and in the interaction between cultivars and regions. A detailed phylogenic analysis of the tested isolates contained that Serbian PVS belongs to PVSO. Of the 18 Serbian PVS isolates included in this study, eight were grouped into the PVSO cluster and formed a subgroup (O-I) with isolates from the USA, Syria, Korea and Chile. Ten Serbian isolates of PVS together with the isolates from Iran were clustered in a branch of subgroup O-VII. This study constitutes the fast report of PVS isolates in Serbia which are capable of infecting Chenopodium quinoa and inducing the symptoms of local chlorotic lesions.
PB  - Springer, Dordrecht
T2  - Potato Research
T1  - The Incidence and Genetic Diversity of Potato virus S in Serbian Seed Potato Crops
EP  - 46
IS  - 1
SP  - 31
VL  - 62
DO  - 10.1007/s11540-018-9395-y
ER  - 

@article{
author = "Ristić, Danijela and Vučurović, Ivan and Kuzmanović, Slobodan and Pfaf-Dolovac, Erika and Aleksić, Goran and Vučurović, Ana and Starović, Mira",
year = "2019",
abstract = "It is essential that certified potatoes are free from known viruses which can negatively affect quality and yield. However, very little is known about the distribution and frequency of Potato virus S (PVS) in Serbia. Until 2014, PVS was present sporadically in the domestic seed potato production. The incidence of PVS was studied by a molecular method over 3 years (2014-2016) in four important potato-growing regions (Moravidd. Zlatiborski, Raski and Macvanski) and in different cultivars. The results showed that the incidence of PVS increased steadily over 3 years from 1.52 to 8.84%. The Moravicki region had the highest incidence (13.06%) and Desiree was the most susceptible cultivar with a mean PVS incidence of 8.2% followed by Marabel and Riviera. The highest significant statistical difference was between the cultivars and in the interaction between cultivars and regions. A detailed phylogenic analysis of the tested isolates contained that Serbian PVS belongs to PVSO. Of the 18 Serbian PVS isolates included in this study, eight were grouped into the PVSO cluster and formed a subgroup (O-I) with isolates from the USA, Syria, Korea and Chile. Ten Serbian isolates of PVS together with the isolates from Iran were clustered in a branch of subgroup O-VII. This study constitutes the fast report of PVS isolates in Serbia which are capable of infecting Chenopodium quinoa and inducing the symptoms of local chlorotic lesions.",
publisher = "Springer, Dordrecht",
journal = "Potato Research",
title = "The Incidence and Genetic Diversity of Potato virus S in Serbian Seed Potato Crops",
pages = "46-31",
number = "1",
volume = "62",
doi = "10.1007/s11540-018-9395-y"
}

Ristić, D., Vučurović, I., Kuzmanović, S., Pfaf-Dolovac, E., Aleksić, G., Vučurović, A.,& Starović, M.. (2019). The Incidence and Genetic Diversity of Potato virus S in Serbian Seed Potato Crops. in Potato Research
Springer, Dordrecht., 62(1), 31-46.
https://doi.org/10.1007/s11540-018-9395-y

Ristić D, Vučurović I, Kuzmanović S, Pfaf-Dolovac E, Aleksić G, Vučurović A, Starović M. The Incidence and Genetic Diversity of Potato virus S in Serbian Seed Potato Crops. in Potato Research. 2019;62(1):31-46.
doi:10.1007/s11540-018-9395-y .

Ristić, Danijela, Vučurović, Ivan, Kuzmanović, Slobodan, Pfaf-Dolovac, Erika, Aleksić, Goran, Vučurović, Ana, Starović, Mira, "The Incidence and Genetic Diversity of Potato virus S in Serbian Seed Potato Crops" in Potato Research, 62, no. 1 (2019):31-46,
https://doi.org/10.1007/s11540-018-9395-y . .

TY  - JOUR
AU  - Stevanović, Miloš
AU  - Ristić, Danijela
AU  - Živković, Svetlana
AU  - Aleksić, Goran
AU  - Stanković, Ivana
AU  - Krstić, Branka
AU  - Bulajić, Aleksandra
PY  - 2019
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/570
AB  - Blackberry cane diseases with the symptoms of necrosis, canker, and wilting are caused by several fungi worldwide. Surveys conducted from 2013 to 2016 in Serbia revealed the occurrence of Gnomoniopsis idaeicola, the causal agent of cane canker and wilting, which was found to be distributed in almost half of the surveyed orchards, in three blackberry cultivars, and with disease incidence of up to 80%. Wide distribution and high disease incidence suggest that G. idaeicola has been present in Serbia for some time. Out of 427 samples, a total of 65 G. idaeicola isolates were obtained (isolation rate of 34.19%). Reference isolates, originating from different localities, were conventionally and molecularly identified and characterized. G. idaeicola was detected in single and mixed infections with fungi from genera Paraconiothyrium, Colletotrichum, Diaporthe, Botryosphaeria, Botrytis, Septoria, Neofusicoccum, and Discostroma, and no diagnostically specific symptoms could be related directly to the G. idaeicola infection. In orchards solely infected with G. idaeicola, blackberry plant mortality was up to 40%, and yield loses were estimated at 50%. G. idaeicola isolates included in this study demonstrated intraspecies diversity in morphological, biological, pathogenic, and molecular features, which indicates that population in Serbia may be of different origin. This is the first record of a massive outbreak of G. idaeicola infection, illustrating its capability of harmful influence on blackberry production. This study represents the initial step in studying G. idaeicola as a new blackberry pathogen in Serbia, aiming at developing efficient control measures.
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - Characterization of Gnomoniopsis idaeicola, the Causal Agent of Canker and Wilting of Blackberry in Serbia
EP  - 258
IS  - 2
SP  - 249
VL  - 103
DO  - 10.1094/PDIS-03-18-0516-RE
ER  - 

@article{
author = "Stevanović, Miloš and Ristić, Danijela and Živković, Svetlana and Aleksić, Goran and Stanković, Ivana and Krstić, Branka and Bulajić, Aleksandra",
year = "2019",
abstract = "Blackberry cane diseases with the symptoms of necrosis, canker, and wilting are caused by several fungi worldwide. Surveys conducted from 2013 to 2016 in Serbia revealed the occurrence of Gnomoniopsis idaeicola, the causal agent of cane canker and wilting, which was found to be distributed in almost half of the surveyed orchards, in three blackberry cultivars, and with disease incidence of up to 80%. Wide distribution and high disease incidence suggest that G. idaeicola has been present in Serbia for some time. Out of 427 samples, a total of 65 G. idaeicola isolates were obtained (isolation rate of 34.19%). Reference isolates, originating from different localities, were conventionally and molecularly identified and characterized. G. idaeicola was detected in single and mixed infections with fungi from genera Paraconiothyrium, Colletotrichum, Diaporthe, Botryosphaeria, Botrytis, Septoria, Neofusicoccum, and Discostroma, and no diagnostically specific symptoms could be related directly to the G. idaeicola infection. In orchards solely infected with G. idaeicola, blackberry plant mortality was up to 40%, and yield loses were estimated at 50%. G. idaeicola isolates included in this study demonstrated intraspecies diversity in morphological, biological, pathogenic, and molecular features, which indicates that population in Serbia may be of different origin. This is the first record of a massive outbreak of G. idaeicola infection, illustrating its capability of harmful influence on blackberry production. This study represents the initial step in studying G. idaeicola as a new blackberry pathogen in Serbia, aiming at developing efficient control measures.",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "Characterization of Gnomoniopsis idaeicola, the Causal Agent of Canker and Wilting of Blackberry in Serbia",
pages = "258-249",
number = "2",
volume = "103",
doi = "10.1094/PDIS-03-18-0516-RE"
}

Stevanović, M., Ristić, D., Živković, S., Aleksić, G., Stanković, I., Krstić, B.,& Bulajić, A.. (2019). Characterization of Gnomoniopsis idaeicola, the Causal Agent of Canker and Wilting of Blackberry in Serbia. in Plant Disease
Amer Phytopathological Soc, St Paul., 103(2), 249-258.
https://doi.org/10.1094/PDIS-03-18-0516-RE

Stevanović M, Ristić D, Živković S, Aleksić G, Stanković I, Krstić B, Bulajić A. Characterization of Gnomoniopsis idaeicola, the Causal Agent of Canker and Wilting of Blackberry in Serbia. in Plant Disease. 2019;103(2):249-258.
doi:10.1094/PDIS-03-18-0516-RE .

Stevanović, Miloš, Ristić, Danijela, Živković, Svetlana, Aleksić, Goran, Stanković, Ivana, Krstić, Branka, Bulajić, Aleksandra, "Characterization of Gnomoniopsis idaeicola, the Causal Agent of Canker and Wilting of Blackberry in Serbia" in Plant Disease, 103, no. 2 (2019):249-258,
https://doi.org/10.1094/PDIS-03-18-0516-RE . .