TY  - CONF
AU  - Stošić, Stefan
AU  - Zivkovic, Svetlana
PY  - 2023
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/1192
AB  - The genus Talaromyces encompasses a wide range of species that are ubiquitous in nature, thriving in
diverse habitats such as soil, plant debris, and indoor environments. They inhabit different climatic zones
across the globe and play crucial roles in natural ecosystems, through participation in organic matter
decomposition and nutrient cycling. Members of this genus are also important enzyme, pigment and
mycotoxin producers, biocontrol agents, human pathogens and food contaminants (1). Talaromyces spp.
can be plant pathogens and few studies pointed that out (2-5). A polyphasic approach was employed to
identify isolates of Talaromyces originating from diseased stored fruits in our country and subsequently
determine their plant pathogenicity. Following protocols recommended by Yilmaz et al. (1), morphological
examinations were carried out on Czapek yeast autolysate agar (CYA), Malt extract agar (MEA) and
Creatine sucrose agar (CREA). Mycelial growth was also tested at three incubation temperatures (5, 25 and
37°C) while micromorphological features were inspected from MEA-grown cultures. Internal transcribed
spacer (ITS) and beta-tubulin (BenA) regions were used for multilocus sequence typing (1). Molecular
sequences from our isolates were combined with the reference and verified sequences to construct a
combined (ITS+BenA) maximum likelihood phylogenetic tree. A pathogenicity test was conducted on
originating hosts. After combining the results from the identification procedures, two species of
Talaromyces were isolated in this study - Talaromyces minioluteus from pear, quince, tomato and orange
fruits, and T. rugulosus from pear and lemon fruits. Phenotypic characteristics were similar to the previous
descriptions in the literature (1, 6, 7). In pathogenicity assays, all isolates were pathogenic on the originating
hosts. The symptoms in artificial inoculations resembled those found in naturally infected fruits and the
reisolates exhibited the same morphological traits as the original isolates. These are the first detections of
T. minioluteus and T. rugulosus and the first confirmations of their pathogenicity on the beforementioned
plant hosts. The research presented here sheds light on the previously known fungi that can also act as plant
pathogens/food spoilage agents. Their presence on the fruit diminishes its quality and leads to economic
damage. Since different fruit and vegetables are sometimes kept in the same storage facilities, these plant
pathogens residing on them can also contribute to inoculum build-up and cross-contamination phenomena.
PB  - University of Perugia
C3  - XIX Congress of European Mycologists, Perugia, Italy, 4-8 September 2023
T1  - Talaromyces spp. - the postharvest fruit pathogens in Serbia
SP  - 71
ER  - 

@conference{
author = "Stošić, Stefan and Zivkovic, Svetlana",
year = "2023",
abstract = "The genus Talaromyces encompasses a wide range of species that are ubiquitous in nature, thriving in
diverse habitats such as soil, plant debris, and indoor environments. They inhabit different climatic zones
across the globe and play crucial roles in natural ecosystems, through participation in organic matter
decomposition and nutrient cycling. Members of this genus are also important enzyme, pigment and
mycotoxin producers, biocontrol agents, human pathogens and food contaminants (1). Talaromyces spp.
can be plant pathogens and few studies pointed that out (2-5). A polyphasic approach was employed to
identify isolates of Talaromyces originating from diseased stored fruits in our country and subsequently
determine their plant pathogenicity. Following protocols recommended by Yilmaz et al. (1), morphological
examinations were carried out on Czapek yeast autolysate agar (CYA), Malt extract agar (MEA) and
Creatine sucrose agar (CREA). Mycelial growth was also tested at three incubation temperatures (5, 25 and
37°C) while micromorphological features were inspected from MEA-grown cultures. Internal transcribed
spacer (ITS) and beta-tubulin (BenA) regions were used for multilocus sequence typing (1). Molecular
sequences from our isolates were combined with the reference and verified sequences to construct a
combined (ITS+BenA) maximum likelihood phylogenetic tree. A pathogenicity test was conducted on
originating hosts. After combining the results from the identification procedures, two species of
Talaromyces were isolated in this study - Talaromyces minioluteus from pear, quince, tomato and orange
fruits, and T. rugulosus from pear and lemon fruits. Phenotypic characteristics were similar to the previous
descriptions in the literature (1, 6, 7). In pathogenicity assays, all isolates were pathogenic on the originating
hosts. The symptoms in artificial inoculations resembled those found in naturally infected fruits and the
reisolates exhibited the same morphological traits as the original isolates. These are the first detections of
T. minioluteus and T. rugulosus and the first confirmations of their pathogenicity on the beforementioned
plant hosts. The research presented here sheds light on the previously known fungi that can also act as plant
pathogens/food spoilage agents. Their presence on the fruit diminishes its quality and leads to economic
damage. Since different fruit and vegetables are sometimes kept in the same storage facilities, these plant
pathogens residing on them can also contribute to inoculum build-up and cross-contamination phenomena.",
publisher = "University of Perugia",
journal = "XIX Congress of European Mycologists, Perugia, Italy, 4-8 September 2023",
title = "Talaromyces spp. - the postharvest fruit pathogens in Serbia",
pages = "71"
}

Stošić, S.,& Zivkovic, S.. (2023). Talaromyces spp. - the postharvest fruit pathogens in Serbia. in XIX Congress of European Mycologists, Perugia, Italy, 4-8 September 2023
University of Perugia., 71.

Stošić S, Zivkovic S. Talaromyces spp. - the postharvest fruit pathogens in Serbia. in XIX Congress of European Mycologists, Perugia, Italy, 4-8 September 2023. 2023;:71..

Stošić, Stefan, Zivkovic, Svetlana, "Talaromyces spp. - the postharvest fruit pathogens in Serbia" in XIX Congress of European Mycologists, Perugia, Italy, 4-8 September 2023 (2023):71.

TY  - CONF
AU  - Živković, Svetlana
AU  - Trkulja, Nenad
AU  - Kovačević, Stefan
AU  - Stošić, Stefan
PY  - 2023
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/1202
AB  - Kruška (Pyrus communis L.) je jedna od najstarijih i najrasprostranjenijih voćarskih
kultura. Plodovi kruške su izrazito bogati nutrijentima i niskog glikemijskog indeksa, pa se
preporučuju u ishrani svih kategorija stanovništva. U periodu vegetacije i nakon berbe
propadanje plodova kruške mogu izazvati brojni biljni patogeni, među kojima su i gljive roda
Colletotricum.
Tokom jeseni 2021. godine iz skladišta u okolini Smedereva sakupljeni su plodovi
kruške (cv. Santa Maria) sa simptomima antraknoze. Na površini svih inficiranih plodova bile
su prisutne tamne, kružne, udubljene nekrotične lezije sa koncentrično raspoređenim
acervulima i narandžastom konidijalnom masom. Izolacija patogena je obavljena standarnim
fitopatološkim metodama na podlozi od krompir-dekstroznog agara (KDA). Svi dobijeni izolati
su nakon 7 dana na KDA i temperaturi od 25⁰C ispoljili uniformne morfološke osobine.
Formirane kolonije su bile svetlosive do svetloružičaste, sa naličja tamnoružičaste do
vinastocrvene boje. Prosečan porast kolonija je iznosio 64±2 mm. Konidije su bile hijalinske,
fuziformne, neseptirane, glatkih zidova, sa oba zaoštrena kraja, veličine 12,8 - 16,6 × 3,5 – 4,4
µm (n=100); a apresorije pojedinačne, tamnobraon boje, okrugle do nepravilnog oblika,
glatkih zidova, veličine 5,5 – 9,6 × 4,8 – 7,2 µm (n=20). Morfološke karakteristike izolata su
odgovarale literaturnom opisu C. acutatum species complex.
Molekularna identifikacija reprezentativnog izolata SZ-21-36 je obavljena na osnovu
ITS regiona rDNA korišćenjem prajmera ITS1/ITS4; dela gena za beta tubulin (Ben A)
primenom prajmera Bt2a/Bt2b; gliceraldehid 3 - fosfat dehidrogenaze (GAPDH) sa
prajmerima GDF1/GDR1, i dela aktin gena (ACT) upotrebom prajmera ACT512F/ACT783R.
Dobijeni PCR produkti su sekvencirani, a nukleotidne sekvence deponovane u GenBank bazi
podataka. BLAST analizom je utvrđeno da su sekvence ON171625 (ITS) i ON186698 (ACT)
99% slične, a sekvence ON186696 (BenA) i ON186697 (GAPDH) identične sa sekvencama
istih regiona izolata vrste C. fioriniae deponovanih u GenBank bazi. Multilokus filogenetska
analiza zasnovana na navedenim molekularnim markerima je potvrdila rezultate BLAST-a, i
svrstala izolat SZ-21-36 sa ostalim izolatima C. fioriniae iz GenBank baze podataka (100%
bootstrap podrška).
Provera patogenosti izolata obavljena je inokulacijom sa 50 μl suspenzije spora (1 ×
106 konidija/ml) u prethodno napravljene povrede na zdravim plodovima kruške (cv. Santa
Maria). Kontrolni plodovi su inokulisani sterilnom destilovanom vodom. Nakon 10 dana
inkubacije u vlažnoj komori na 25°C na svim inokulisanim plodovima kruške razvili su se
tipični simptomi antraknoze, dok su kontrolni plodovi bili bez simptoma. Kulture dobijene  reizolacijom bile su istih morfoloških karakteristika čime je potvrđena patogenost testiranih
izolata.
U ovom istraživanju je konvencionalnim i molekularnim metodama prvi put u Srbiji
identifikovana vrsta C. fioriniae - prouzrokovač antraknoze plodova kruške.
PB  - Društvo za zaštitu bilja
C3  - XVII simpozijum o zaštiti bilja, Zlatibor 2023, 27-30. novembar, Zbornik rezimea radova
T1  - Colletotrichum fioriniae  prouzrokovač antraknoze plodova kruške
EP  - 45
SP  - 44
ER  - 

@conference{
author = "Živković, Svetlana and Trkulja, Nenad and Kovačević, Stefan and Stošić, Stefan",
year = "2023",
abstract = "Kruška (Pyrus communis L.) je jedna od najstarijih i najrasprostranjenijih voćarskih
kultura. Plodovi kruške su izrazito bogati nutrijentima i niskog glikemijskog indeksa, pa se
preporučuju u ishrani svih kategorija stanovništva. U periodu vegetacije i nakon berbe
propadanje plodova kruške mogu izazvati brojni biljni patogeni, među kojima su i gljive roda
Colletotricum.
Tokom jeseni 2021. godine iz skladišta u okolini Smedereva sakupljeni su plodovi
kruške (cv. Santa Maria) sa simptomima antraknoze. Na površini svih inficiranih plodova bile
su prisutne tamne, kružne, udubljene nekrotične lezije sa koncentrično raspoređenim
acervulima i narandžastom konidijalnom masom. Izolacija patogena je obavljena standarnim
fitopatološkim metodama na podlozi od krompir-dekstroznog agara (KDA). Svi dobijeni izolati
su nakon 7 dana na KDA i temperaturi od 25⁰C ispoljili uniformne morfološke osobine.
Formirane kolonije su bile svetlosive do svetloružičaste, sa naličja tamnoružičaste do
vinastocrvene boje. Prosečan porast kolonija je iznosio 64±2 mm. Konidije su bile hijalinske,
fuziformne, neseptirane, glatkih zidova, sa oba zaoštrena kraja, veličine 12,8 - 16,6 × 3,5 – 4,4
µm (n=100); a apresorije pojedinačne, tamnobraon boje, okrugle do nepravilnog oblika,
glatkih zidova, veličine 5,5 – 9,6 × 4,8 – 7,2 µm (n=20). Morfološke karakteristike izolata su
odgovarale literaturnom opisu C. acutatum species complex.
Molekularna identifikacija reprezentativnog izolata SZ-21-36 je obavljena na osnovu
ITS regiona rDNA korišćenjem prajmera ITS1/ITS4; dela gena za beta tubulin (Ben A)
primenom prajmera Bt2a/Bt2b; gliceraldehid 3 - fosfat dehidrogenaze (GAPDH) sa
prajmerima GDF1/GDR1, i dela aktin gena (ACT) upotrebom prajmera ACT512F/ACT783R.
Dobijeni PCR produkti su sekvencirani, a nukleotidne sekvence deponovane u GenBank bazi
podataka. BLAST analizom je utvrđeno da su sekvence ON171625 (ITS) i ON186698 (ACT)
99% slične, a sekvence ON186696 (BenA) i ON186697 (GAPDH) identične sa sekvencama
istih regiona izolata vrste C. fioriniae deponovanih u GenBank bazi. Multilokus filogenetska
analiza zasnovana na navedenim molekularnim markerima je potvrdila rezultate BLAST-a, i
svrstala izolat SZ-21-36 sa ostalim izolatima C. fioriniae iz GenBank baze podataka (100%
bootstrap podrška).
Provera patogenosti izolata obavljena je inokulacijom sa 50 μl suspenzije spora (1 ×
106 konidija/ml) u prethodno napravljene povrede na zdravim plodovima kruške (cv. Santa
Maria). Kontrolni plodovi su inokulisani sterilnom destilovanom vodom. Nakon 10 dana
inkubacije u vlažnoj komori na 25°C na svim inokulisanim plodovima kruške razvili su se
tipični simptomi antraknoze, dok su kontrolni plodovi bili bez simptoma. Kulture dobijene  reizolacijom bile su istih morfoloških karakteristika čime je potvrđena patogenost testiranih
izolata.
U ovom istraživanju je konvencionalnim i molekularnim metodama prvi put u Srbiji
identifikovana vrsta C. fioriniae - prouzrokovač antraknoze plodova kruške.",
publisher = "Društvo za zaštitu bilja",
journal = "XVII simpozijum o zaštiti bilja, Zlatibor 2023, 27-30. novembar, Zbornik rezimea radova",
title = "Colletotrichum fioriniae  prouzrokovač antraknoze plodova kruške",
pages = "45-44"
}

Živković, S., Trkulja, N., Kovačević, S.,& Stošić, S.. (2023). Colletotrichum fioriniae  prouzrokovač antraknoze plodova kruške. in XVII simpozijum o zaštiti bilja, Zlatibor 2023, 27-30. novembar, Zbornik rezimea radova
Društvo za zaštitu bilja., 44-45.

Živković S, Trkulja N, Kovačević S, Stošić S. Colletotrichum fioriniae  prouzrokovač antraknoze plodova kruške. in XVII simpozijum o zaštiti bilja, Zlatibor 2023, 27-30. novembar, Zbornik rezimea radova. 2023;:44-45..

Živković, Svetlana, Trkulja, Nenad, Kovačević, Stefan, Stošić, Stefan, "Colletotrichum fioriniae  prouzrokovač antraknoze plodova kruške" in XVII simpozijum o zaštiti bilja, Zlatibor 2023, 27-30. novembar, Zbornik rezimea radova (2023):44-45.

Živković, S., Trkulja, N., Kovačević, S.,& Stošić, S.. (2023). First Report of Colletotrichum fioriniae Causing Anthracnose on Pear Fruit in Serbia. in Plant Disease
American Phytopathological Society..
https://doi.org/10.1094/PDIS-05-22-1019-PDN

Živković S, Trkulja N, Kovačević S, Stošić S. First Report of Colletotrichum fioriniae Causing Anthracnose on Pear Fruit in Serbia. in Plant Disease. 2023;.
doi:10.1094/PDIS-05-22-1019-PDN .

Živković, Svetlana, Trkulja, Nenad, Kovačević, Stefan, Stošić, Stefan, "First Report of Colletotrichum fioriniae Causing Anthracnose on Pear Fruit in Serbia" in Plant Disease (2023),
https://doi.org/10.1094/PDIS-05-22-1019-PDN . .

TY  - CONF
AU  - Zivkovic, Svetlana
AU  - Ristić, Lela
AU  - Starović, Mira
AU  - Aleksic, Goran
AU  - Stošić, Stefan
PY  - 2022
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/791
AB  - Tomato (Solanum lycopersicum, L.) is one of the most widely cultivated crops with high content of vitamins and antioxidant lycopene, which are very important for human health. During the growing season and postharvest storage tomato is susceptible to various diseases caused by pathogenic fungi. In July 2019, tomato (cv. Balkan) with symptoms of blue mold decay were collected from market in Belgrade, Serbia. Macroscopic morphology of three obtained monosporic isolates were observed after growth on Czapek yeast autolysate agar (CYA), creatine sucrose agar (CREA), and malt extract agar (MEA) for seven days at 25ºC. Also, selected isolates were incubated at 5, 25, and 37°C for one week on CYA to monitor the effect of different temperature incubation conditions. Colony characteristics and micromorphology of the fungi agreed with the literature descriptions of Penicillium expansum. The conidiophores of isolates were hyaline, mainly terverticillate; stipes usually smooth-walled; metulae and phialides cylindrical; conidia ellipsoidal (3-3,86-4 × 3-3,13-4 μm), greenish, smooth-walled. Total DNA was extracted using DNeasy Plant Mini Kit (Qiagen, Hilden, Germany) and partial β-tubulin (BenA) sequence was amplified with primers Bt2a/Bt2b. BenA sequence of representative isolate ParP/1 was deposited in NCBI GenBank (Accession No. ON186699). Phylogenetic analysis clustered our isolate with other isolates of P. expansum. Pathogenicity test was conducted on symptomless, detached tomato fruits. All tested isolates caused typical blue mold symptoms on tomato fruits after seven days of incubation. To our knowledge, this is the first report of P. expansum causing postharvest fruit decay on tomato in Serbia.
PB  - Univerisity of East Sarajevo, Faculty of Agriculture
C3  - Proceedings of the XIII International Scientific Agricultural Symposium “Agrosym 2022” 6-9 oktobar 2022, 545 - 551
T1  - Penicillium expansum as a postharvest pathogen of tomato fruit in Serbia
ER  - 

@conference{
author = "Zivkovic, Svetlana and Ristić, Lela and Starović, Mira and Aleksic, Goran and Stošić, Stefan",
year = "2022",
abstract = "Tomato (Solanum lycopersicum, L.) is one of the most widely cultivated crops with high content of vitamins and antioxidant lycopene, which are very important for human health. During the growing season and postharvest storage tomato is susceptible to various diseases caused by pathogenic fungi. In July 2019, tomato (cv. Balkan) with symptoms of blue mold decay were collected from market in Belgrade, Serbia. Macroscopic morphology of three obtained monosporic isolates were observed after growth on Czapek yeast autolysate agar (CYA), creatine sucrose agar (CREA), and malt extract agar (MEA) for seven days at 25ºC. Also, selected isolates were incubated at 5, 25, and 37°C for one week on CYA to monitor the effect of different temperature incubation conditions. Colony characteristics and micromorphology of the fungi agreed with the literature descriptions of Penicillium expansum. The conidiophores of isolates were hyaline, mainly terverticillate; stipes usually smooth-walled; metulae and phialides cylindrical; conidia ellipsoidal (3-3,86-4 × 3-3,13-4 μm), greenish, smooth-walled. Total DNA was extracted using DNeasy Plant Mini Kit (Qiagen, Hilden, Germany) and partial β-tubulin (BenA) sequence was amplified with primers Bt2a/Bt2b. BenA sequence of representative isolate ParP/1 was deposited in NCBI GenBank (Accession No. ON186699). Phylogenetic analysis clustered our isolate with other isolates of P. expansum. Pathogenicity test was conducted on symptomless, detached tomato fruits. All tested isolates caused typical blue mold symptoms on tomato fruits after seven days of incubation. To our knowledge, this is the first report of P. expansum causing postharvest fruit decay on tomato in Serbia.",
publisher = "Univerisity of East Sarajevo, Faculty of Agriculture",
journal = "Proceedings of the XIII International Scientific Agricultural Symposium “Agrosym 2022” 6-9 oktobar 2022, 545 - 551",
title = "Penicillium expansum as a postharvest pathogen of tomato fruit in Serbia"
}

Zivkovic, S., Ristić, L., Starović, M., Aleksic, G.,& Stošić, S.. (2022). Penicillium expansum as a postharvest pathogen of tomato fruit in Serbia. in Proceedings of the XIII International Scientific Agricultural Symposium “Agrosym 2022” 6-9 oktobar 2022, 545 - 551
Univerisity of East Sarajevo, Faculty of Agriculture..

Zivkovic S, Ristić L, Starović M, Aleksic G, Stošić S. Penicillium expansum as a postharvest pathogen of tomato fruit in Serbia. in Proceedings of the XIII International Scientific Agricultural Symposium “Agrosym 2022” 6-9 oktobar 2022, 545 - 551. 2022;..

Zivkovic, Svetlana, Ristić, Lela, Starović, Mira, Aleksic, Goran, Stošić, Stefan, "Penicillium expansum as a postharvest pathogen of tomato fruit in Serbia" in Proceedings of the XIII International Scientific Agricultural Symposium “Agrosym 2022” 6-9 oktobar 2022, 545 - 551 (2022).

TY  - JOUR
AU  - Zlatković, Nevena
AU  - Gašić, Katarina
AU  - Kuzmanović, Nemanja
AU  - Prokić, Anđelka
AU  - Ivanović, Milan
AU  - Zivkovic, Svetlana
AU  - Obradović, Aleksa
PY  - 2022
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/744
AB  - Acidovorax citrulli, the causal agent of bacterial fruit blotch (BFB), is an economically
important pathogen of watermelon and related plant species worldwide. In the period 2014–2018,
several outbreaks of BFB were observed in major watermelon production regions in Serbia. A total of
43 strains, isolated from symptomatic watermelon tissue, were analyzed by biochemical, pathogenic
and molecular tests. Based on the phenotypic characteristics, PCR assay, and 16S rRNA gene
sequence analysis, all strains were identified as A. citrulli. A multilocus sequence analysis of the four
housekeeping (adk, gyrB, pilT and gltA) and three virulence genes (Aave_1548, avrRx01 and luxR)
revealed that studied A. citrulli strains represent the homogeneous population and they clustered
together with group II reference strain AAC00-1. Strain affiliation to group II was confirmed by PCR
based on the putative type III secretion effector gene and by duplex PCR test. High homogeneity
of studied strains was also confirmed by BOX-PCR. Differences were observed for two strains in
their pathogenicity as well as susceptibility to copper compounds. Moreover, six major watermelon
varieties grown in Serbia showed high sensitivity to the pathogen, while cross inoculation assay
revealed that the strains were able to infect other species within the Cucurbitaceae family.
PB  - MDPI
T2  - Agronomy
T1  - Polyphasic Characterization of Acidovorax citrulli Strains Originating from Serbia
SP  - 235
VL  - 12
VL  - 2
DO  - 10.3390/agronomy12020235
ER  - 

@article{
author = "Zlatković, Nevena and Gašić, Katarina and Kuzmanović, Nemanja and Prokić, Anđelka and Ivanović, Milan and Zivkovic, Svetlana and Obradović, Aleksa",
year = "2022",
abstract = "Acidovorax citrulli, the causal agent of bacterial fruit blotch (BFB), is an economically
important pathogen of watermelon and related plant species worldwide. In the period 2014–2018,
several outbreaks of BFB were observed in major watermelon production regions in Serbia. A total of
43 strains, isolated from symptomatic watermelon tissue, were analyzed by biochemical, pathogenic
and molecular tests. Based on the phenotypic characteristics, PCR assay, and 16S rRNA gene
sequence analysis, all strains were identified as A. citrulli. A multilocus sequence analysis of the four
housekeeping (adk, gyrB, pilT and gltA) and three virulence genes (Aave_1548, avrRx01 and luxR)
revealed that studied A. citrulli strains represent the homogeneous population and they clustered
together with group II reference strain AAC00-1. Strain affiliation to group II was confirmed by PCR
based on the putative type III secretion effector gene and by duplex PCR test. High homogeneity
of studied strains was also confirmed by BOX-PCR. Differences were observed for two strains in
their pathogenicity as well as susceptibility to copper compounds. Moreover, six major watermelon
varieties grown in Serbia showed high sensitivity to the pathogen, while cross inoculation assay
revealed that the strains were able to infect other species within the Cucurbitaceae family.",
publisher = "MDPI",
journal = "Agronomy",
title = "Polyphasic Characterization of Acidovorax citrulli Strains Originating from Serbia",
pages = "235",
volume = "12, 2",
doi = "10.3390/agronomy12020235"
}

Zlatković, N., Gašić, K., Kuzmanović, N., Prokić, A., Ivanović, M., Zivkovic, S.,& Obradović, A.. (2022). Polyphasic Characterization of Acidovorax citrulli Strains Originating from Serbia. in Agronomy
MDPI., 12, 235.
https://doi.org/10.3390/agronomy12020235

Zlatković N, Gašić K, Kuzmanović N, Prokić A, Ivanović M, Zivkovic S, Obradović A. Polyphasic Characterization of Acidovorax citrulli Strains Originating from Serbia. in Agronomy. 2022;12:235.
doi:10.3390/agronomy12020235 .

Zlatković, Nevena, Gašić, Katarina, Kuzmanović, Nemanja, Prokić, Anđelka, Ivanović, Milan, Zivkovic, Svetlana, Obradović, Aleksa, "Polyphasic Characterization of Acidovorax citrulli Strains Originating from Serbia" in Agronomy, 12 (2022):235,
https://doi.org/10.3390/agronomy12020235 . .

TY  - CONF
AU  - Starović, Mira
AU  - Ristić, Lela
AU  - Vučurović, Ivan
AU  - Blagojević, Jovana
AU  - Stošić, Stefan
AU  - Živković, Svetlana
AU  - Aleksic, Goran
PY  - 2022
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/787
AB  - Garlic (Allium sativum L.) is the second most cultivated Allium species worldwide. Many viral, fungal and bacterial diseases attack garlic plants and can be a limiting factor to garlic production. Fungal diseases control is mainly conducted by fungicides, which have detrimental impact on the environment. In this work, we explore the possibility of utilizing essential oils (EOs) as antifungal agents against a fungal isolate originated from symptomatic garlic cloves from Kraljevci locality, Srem District, Serbia, morphologically and molecularly identified as Fusarium verticillioides. EOs used in this study originated from several medicinal plants: Turkish pickling herb (Echinophora tenuifolia), oregano (Origanum vulgare), basil (Ocimum basilicum) and myrtle (Myrtus communis). Minimum inhibitory concentrations (MIC) were determined by microdilution method in 96 well microtiter plates. Microtiter plates were incubated for five days at 28°C. The experiment was repeated four times with trifloxystrobin as a positive control. The lowest concentrations without visible growth were defined as the minimal concentrations inhibiting fungal growth. Fungal spores were washed from the surface of potato dextrose agar (PDA) and spore suspension was adjusted to a concentration of approximately 5.0x104 in a final volume of 100μl per well. The values of minimal inhibitory concentration (MIC) were carried out by Duncan’s multiple range tests. An analysis of variance was performed on MIC data for four EOs applied on F. verticillioides. A significance was evaluated at p<0.05. STATISTICA v.7 (StatSoft, Inc.) was used for statistical analyses. The results of the antimicrobial activity of EOs using microdilution method showed a wide range of antifungal activity against F. verticillioides. The basil EO proved to be the most potent one (MIC-0,325±5,10μg/mL), followed by oregano (MIC-0,775±0,05μg/mL), myrtle (MIC-5,5±0,05μg/mL) and Turkish pickling herb (MIC-55±5,10μg/mL). The data obtained here suggest that the selected EOs can be applied as inhibitors to prevent growth of the phytopathogenic fungus F. verticillioides.
PB  - University of East Sarajevo
C3  - Proceedings of the XIII International Scientific Agricultural Symposium “Agrosym October 6-9, 2022
T1  - Antifungal activity of plant essential oils to the Fusarium verticillioides originated from garlic
EP  - 650
IS  - 645
ER  - 

@conference{
author = "Starović, Mira and Ristić, Lela and Vučurović, Ivan and Blagojević, Jovana and Stošić, Stefan and Živković, Svetlana and Aleksic, Goran",
year = "2022",
abstract = "Garlic (Allium sativum L.) is the second most cultivated Allium species worldwide. Many viral, fungal and bacterial diseases attack garlic plants and can be a limiting factor to garlic production. Fungal diseases control is mainly conducted by fungicides, which have detrimental impact on the environment. In this work, we explore the possibility of utilizing essential oils (EOs) as antifungal agents against a fungal isolate originated from symptomatic garlic cloves from Kraljevci locality, Srem District, Serbia, morphologically and molecularly identified as Fusarium verticillioides. EOs used in this study originated from several medicinal plants: Turkish pickling herb (Echinophora tenuifolia), oregano (Origanum vulgare), basil (Ocimum basilicum) and myrtle (Myrtus communis). Minimum inhibitory concentrations (MIC) were determined by microdilution method in 96 well microtiter plates. Microtiter plates were incubated for five days at 28°C. The experiment was repeated four times with trifloxystrobin as a positive control. The lowest concentrations without visible growth were defined as the minimal concentrations inhibiting fungal growth. Fungal spores were washed from the surface of potato dextrose agar (PDA) and spore suspension was adjusted to a concentration of approximately 5.0x104 in a final volume of 100μl per well. The values of minimal inhibitory concentration (MIC) were carried out by Duncan’s multiple range tests. An analysis of variance was performed on MIC data for four EOs applied on F. verticillioides. A significance was evaluated at p<0.05. STATISTICA v.7 (StatSoft, Inc.) was used for statistical analyses. The results of the antimicrobial activity of EOs using microdilution method showed a wide range of antifungal activity against F. verticillioides. The basil EO proved to be the most potent one (MIC-0,325±5,10μg/mL), followed by oregano (MIC-0,775±0,05μg/mL), myrtle (MIC-5,5±0,05μg/mL) and Turkish pickling herb (MIC-55±5,10μg/mL). The data obtained here suggest that the selected EOs can be applied as inhibitors to prevent growth of the phytopathogenic fungus F. verticillioides.",
publisher = "University of East Sarajevo",
journal = "Proceedings of the XIII International Scientific Agricultural Symposium “Agrosym October 6-9, 2022",
title = "Antifungal activity of plant essential oils to the Fusarium verticillioides originated from garlic",
pages = "650",
number = "645"
}

Starović, M., Ristić, L., Vučurović, I., Blagojević, J., Stošić, S., Živković, S.,& Aleksic, G.. (2022). Antifungal activity of plant essential oils to the Fusarium verticillioides originated from garlic. in Proceedings of the XIII International Scientific Agricultural Symposium “Agrosym October 6-9, 2022
University of East Sarajevo.(645).

Starović M, Ristić L, Vučurović I, Blagojević J, Stošić S, Živković S, Aleksic G. Antifungal activity of plant essential oils to the Fusarium verticillioides originated from garlic. in Proceedings of the XIII International Scientific Agricultural Symposium “Agrosym October 6-9, 2022. 2022;(645):null-650..

Starović, Mira, Ristić, Lela, Vučurović, Ivan, Blagojević, Jovana, Stošić, Stefan, Živković, Svetlana, Aleksic, Goran, "Antifungal activity of plant essential oils to the Fusarium verticillioides originated from garlic" in Proceedings of the XIII International Scientific Agricultural Symposium “Agrosym October 6-9, 2022, no. 645 (2022).

TY  - JOUR
AU  - Stošić, Stefan
AU  - Delić, Dušica
AU  - Živković, Svetlana
PY  - 2022
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/627
AB  - Lemon fruits are an important source of vitamin C, potassium, folate, carotenoids, polyphenols, coumarins and terpenes. These lemon compounds have antioxidant and anti-inflammatory properties which have beneficial effects on human health. This re- search aimed to elucidate the etiology of blue and green molds detected on lemon fruits in Serbia. Using integrative identification approach, the obtained isolates were characterized from morphological, physiological, molecular, phylogenetic and pathological aspects. Col- ony growth and morphology were examined on Czapek yeast autolysate agar (CYA), Malt extract agar (MEA) and Creatine sucrose agar (CREA), and on CYA at two additional incu- bation temperatures (5 and 37 °C). For molecular identification, ITS and partial β-tubulin (BenA) genes were sequenced. Phylogenetic relationships were investigated using maximumlikelihood method. A pathogenicity test was carried out and the possible difference in pathogenicity among isolates was assessed with analysis of variance (ANOVA) and subsequent Tukey’s test. Four species were identified: Penicillium expansum, Penicillium digitatum, Penicillium polonicum and Talaromyces rugulosus. All four species proved to be pathogenic on lemon fruits, producing symptoms similar to those observed on naturally infected fruits. The results of this study are the first records of the beforementioned Penicillium/ Talaromyces species as postharvest pathogens on lemon fruits in Serbia and the first world report of T. rugulosus as phytopathogenic on the same host.
PB  - Matica Srpska
T2  - Zbornik Matice srpske za prirodne nauke
T1  - Polyphasic identification of decay agents of lemon fruits in Serbia
SP  - 73
SP  - 87
VL  - 143
DO  - 10.2298/ZMSPN2243073S
ER  - 

@article{
author = "Stošić, Stefan and Delić, Dušica and Živković, Svetlana",
year = "2022",
abstract = "Lemon fruits are an important source of vitamin C, potassium, folate, carotenoids, polyphenols, coumarins and terpenes. These lemon compounds have antioxidant and anti-inflammatory properties which have beneficial effects on human health. This re- search aimed to elucidate the etiology of blue and green molds detected on lemon fruits in Serbia. Using integrative identification approach, the obtained isolates were characterized from morphological, physiological, molecular, phylogenetic and pathological aspects. Col- ony growth and morphology were examined on Czapek yeast autolysate agar (CYA), Malt extract agar (MEA) and Creatine sucrose agar (CREA), and on CYA at two additional incu- bation temperatures (5 and 37 °C). For molecular identification, ITS and partial β-tubulin (BenA) genes were sequenced. Phylogenetic relationships were investigated using maximumlikelihood method. A pathogenicity test was carried out and the possible difference in pathogenicity among isolates was assessed with analysis of variance (ANOVA) and subsequent Tukey’s test. Four species were identified: Penicillium expansum, Penicillium digitatum, Penicillium polonicum and Talaromyces rugulosus. All four species proved to be pathogenic on lemon fruits, producing symptoms similar to those observed on naturally infected fruits. The results of this study are the first records of the beforementioned Penicillium/ Talaromyces species as postharvest pathogens on lemon fruits in Serbia and the first world report of T. rugulosus as phytopathogenic on the same host.",
publisher = "Matica Srpska",
journal = "Zbornik Matice srpske za prirodne nauke",
title = "Polyphasic identification of decay agents of lemon fruits in Serbia",
pages = "73-87",
volume = "143",
doi = "10.2298/ZMSPN2243073S"
}

Stošić, S., Delić, D.,& Živković, S.. (2022). Polyphasic identification of decay agents of lemon fruits in Serbia. in Zbornik Matice srpske za prirodne nauke
Matica Srpska., 143, 73.
https://doi.org/10.2298/ZMSPN2243073S

Stošić S, Delić D, Živković S. Polyphasic identification of decay agents of lemon fruits in Serbia. in Zbornik Matice srpske za prirodne nauke. 2022;143:73.
doi:10.2298/ZMSPN2243073S .

Stošić, Stefan, Delić, Dušica, Živković, Svetlana, "Polyphasic identification of decay agents of lemon fruits in Serbia" in Zbornik Matice srpske za prirodne nauke, 143 (2022):73,
https://doi.org/10.2298/ZMSPN2243073S . .

TY  - JOUR
AU  - Stošić, Stefan
AU  - Ristić, Lela
AU  - Savković, Željko
AU  - Ljaljevic Grbic, Milica
AU  - Vukojevic, Jelena
AU  - Živković, Svetlana
PY  - 2021
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/726
AB  - Pears are one of the oldest and the third most important fruit species grown in temperate regions. They are consumed because of their nutritional and health benefits, in fresh form or as various processed products. This article resolves the etiology of the Penicillium-like mold symptoms on pear fruits in Serbia. Samples of pear fruits with blue mold and other Penicillium-like mold symptoms were collected in Serbia from 2016 to 2019, from four storages. The recovered isolates were identified and characterized according to a polyphasic approach. Morphological and physiological analyses were performed on three media and five temperatures, respectively. Four loci (internal transcribed spacer, beta-tubulin, calmodulin, and DNA-dependent RNA polymerase II second largest subunit) were used for sequencing, genetic identification, and phylogenetic analyses. The results of the identification by conventional and molecular methods were in agreement, and they revealed that the obtained isolates belong to five species: Penicillium crustosum, P. expansum, P. italicum, Talaromyces minioluteus, and T. rugulosus. In a pathogenicity test, P. crustosum, P. expansum, T. minioluteus, and T. rugulosus produced decay on artificially inoculated pear fruits, and P. italicum induced tissue response lesions. The results of this study are the first reports of T. minioluteus and T. rugulosus as postharvest pear pathogens. Also, these are the first world records of T. minioluteus, T. rugulosus, and P. italicum on fruits of European pear. Furthermore, this is the first finding of P. crustosum, P. expansum, P. italicum, T. minioluteus, and T. rugulosus on pear fruit in Serbia.
PB  - American Phytopathological Society
T2  - Plant Disease
T1  - Penicillium and Talaromyces Species as Postharvest Pathogens of Pear Fruit (Pyrus communis) in Serbia
EP  - 3521
IS  - 11
SP  - 3510
VL  - 105
DO  - 10.1094/PDIS-01-21-0037-RE
ER  - 

@article{
author = "Stošić, Stefan and Ristić, Lela and Savković, Željko and Ljaljevic Grbic, Milica and Vukojevic, Jelena and Živković, Svetlana",
year = "2021",
abstract = "Pears are one of the oldest and the third most important fruit species grown in temperate regions. They are consumed because of their nutritional and health benefits, in fresh form or as various processed products. This article resolves the etiology of the Penicillium-like mold symptoms on pear fruits in Serbia. Samples of pear fruits with blue mold and other Penicillium-like mold symptoms were collected in Serbia from 2016 to 2019, from four storages. The recovered isolates were identified and characterized according to a polyphasic approach. Morphological and physiological analyses were performed on three media and five temperatures, respectively. Four loci (internal transcribed spacer, beta-tubulin, calmodulin, and DNA-dependent RNA polymerase II second largest subunit) were used for sequencing, genetic identification, and phylogenetic analyses. The results of the identification by conventional and molecular methods were in agreement, and they revealed that the obtained isolates belong to five species: Penicillium crustosum, P. expansum, P. italicum, Talaromyces minioluteus, and T. rugulosus. In a pathogenicity test, P. crustosum, P. expansum, T. minioluteus, and T. rugulosus produced decay on artificially inoculated pear fruits, and P. italicum induced tissue response lesions. The results of this study are the first reports of T. minioluteus and T. rugulosus as postharvest pear pathogens. Also, these are the first world records of T. minioluteus, T. rugulosus, and P. italicum on fruits of European pear. Furthermore, this is the first finding of P. crustosum, P. expansum, P. italicum, T. minioluteus, and T. rugulosus on pear fruit in Serbia.",
publisher = "American Phytopathological Society",
journal = "Plant Disease",
title = "Penicillium and Talaromyces Species as Postharvest Pathogens of Pear Fruit (Pyrus communis) in Serbia",
pages = "3521-3510",
number = "11",
volume = "105",
doi = "10.1094/PDIS-01-21-0037-RE"
}

Stošić, S., Ristić, L., Savković, Ž., Ljaljevic Grbic, M., Vukojevic, J.,& Živković, S.. (2021). Penicillium and Talaromyces Species as Postharvest Pathogens of Pear Fruit (Pyrus communis) in Serbia. in Plant Disease
American Phytopathological Society., 105(11), 3510-3521.
https://doi.org/10.1094/PDIS-01-21-0037-RE

Stošić S, Ristić L, Savković Ž, Ljaljevic Grbic M, Vukojevic J, Živković S. Penicillium and Talaromyces Species as Postharvest Pathogens of Pear Fruit (Pyrus communis) in Serbia. in Plant Disease. 2021;105(11):3510-3521.
doi:10.1094/PDIS-01-21-0037-RE .

Stošić, Stefan, Ristić, Lela, Savković, Željko, Ljaljevic Grbic, Milica, Vukojevic, Jelena, Živković, Svetlana, "Penicillium and Talaromyces Species as Postharvest Pathogens of Pear Fruit (Pyrus communis) in Serbia" in Plant Disease, 105, no. 11 (2021):3510-3521,
https://doi.org/10.1094/PDIS-01-21-0037-RE . .

TY  - CONF
AU  - Zivkovic, Svetlana
AU  - Ristić, Lela
AU  - Starović, Mira
AU  - Aleksic, Goran
AU  - Stošić, Stefan
PY  - 2021
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/817
PB  - Društvo za zaštitu bilja
C3  - XVI simpozijum o zaštiti bilja, Zlatibor 22. do 25. novembra 2021: 66-67
T1  - Antifungalni efekat hitosana prema Talaromyces minioluteus in vitro
ER  - 

@conference{
author = "Zivkovic, Svetlana and Ristić, Lela and Starović, Mira and Aleksic, Goran and Stošić, Stefan",
year = "2021",
publisher = "Društvo za zaštitu bilja",
journal = "XVI simpozijum o zaštiti bilja, Zlatibor 22. do 25. novembra 2021: 66-67",
title = "Antifungalni efekat hitosana prema Talaromyces minioluteus in vitro"
}

Zivkovic, S., Ristić, L., Starović, M., Aleksic, G.,& Stošić, S.. (2021). Antifungalni efekat hitosana prema Talaromyces minioluteus in vitro. in XVI simpozijum o zaštiti bilja, Zlatibor 22. do 25. novembra 2021: 66-67
Društvo za zaštitu bilja..

Zivkovic S, Ristić L, Starović M, Aleksic G, Stošić S. Antifungalni efekat hitosana prema Talaromyces minioluteus in vitro. in XVI simpozijum o zaštiti bilja, Zlatibor 22. do 25. novembra 2021: 66-67. 2021;..

Zivkovic, Svetlana, Ristić, Lela, Starović, Mira, Aleksic, Goran, Stošić, Stefan, "Antifungalni efekat hitosana prema Talaromyces minioluteus in vitro" in XVI simpozijum o zaštiti bilja, Zlatibor 22. do 25. novembra 2021: 66-67 (2021).

TY  - CONF
AU  - Aleksic, Goran
AU  - Milićević, Zoran
AU  - Zivkovic, Svetlana
AU  - Starović, Mira
AU  - Stevanović, Miloš
AU  - Ristić, Lela
AU  - Kuzmanović, Slobodan
PY  - 2021
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/807
PB  - Društvo za zaštitu bilja Srbije
C3  - Zbornik rezimea radova/XVI simpozijum o zaštiti bilja, 22-25. novembar 2021, Zlatibor: 52-53
T1  - Primena bakar citrata u suzbijanju prouzrokovača sive truleži vinove loze – Botrytis cinerea
ER  - 

@conference{
author = "Aleksic, Goran and Milićević, Zoran and Zivkovic, Svetlana and Starović, Mira and Stevanović, Miloš and Ristić, Lela and Kuzmanović, Slobodan",
year = "2021",
publisher = "Društvo za zaštitu bilja Srbije",
journal = "Zbornik rezimea radova/XVI simpozijum o zaštiti bilja, 22-25. novembar 2021, Zlatibor: 52-53",
title = "Primena bakar citrata u suzbijanju prouzrokovača sive truleži vinove loze – Botrytis cinerea"
}

Aleksic, G., Milićević, Z., Zivkovic, S., Starović, M., Stevanović, M., Ristić, L.,& Kuzmanović, S.. (2021). Primena bakar citrata u suzbijanju prouzrokovača sive truleži vinove loze – Botrytis cinerea. in Zbornik rezimea radova/XVI simpozijum o zaštiti bilja, 22-25. novembar 2021, Zlatibor: 52-53
Društvo za zaštitu bilja Srbije..

Aleksic G, Milićević Z, Zivkovic S, Starović M, Stevanović M, Ristić L, Kuzmanović S. Primena bakar citrata u suzbijanju prouzrokovača sive truleži vinove loze – Botrytis cinerea. in Zbornik rezimea radova/XVI simpozijum o zaštiti bilja, 22-25. novembar 2021, Zlatibor: 52-53. 2021;..

Aleksic, Goran, Milićević, Zoran, Zivkovic, Svetlana, Starović, Mira, Stevanović, Miloš, Ristić, Lela, Kuzmanović, Slobodan, "Primena bakar citrata u suzbijanju prouzrokovača sive truleži vinove loze – Botrytis cinerea" in Zbornik rezimea radova/XVI simpozijum o zaštiti bilja, 22-25. novembar 2021, Zlatibor: 52-53 (2021).

Zivkovic, S., Ristić, D.,& Stošić, S.. (2021). First report of Penicillium olsonii causing postharvest fruit rot on tomato in Serbia. in Plant Disease
American Phytopathological Society., 105(8), 2246-2246.
https://doi.org/10.1094/PDIS-02-21-0323-PDN

Zivkovic S, Ristić D, Stošić S. First report of Penicillium olsonii causing postharvest fruit rot on tomato in Serbia. in Plant Disease. 2021;105(8):2246-2246.
doi:10.1094/PDIS-02-21-0323-PDN .

Zivkovic, Svetlana, Ristić, Danijela, Stošić, Stefan, "First report of Penicillium olsonii causing postharvest fruit rot on tomato in Serbia" in Plant Disease, 105, no. 8 (2021):2246-2246,
https://doi.org/10.1094/PDIS-02-21-0323-PDN . .

TY  - CONF
AU  - Zlatković, Nevena
AU  - Gašić, Katarina
AU  - Prokić, Anđelka
AU  - Kuzmanović, Nemanja
AU  - Ivanović, Milan
AU  - Živković, Svetlana
AU  - Obradović, Aleksa
PY  - 2021
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/1276
AB  - Pojava bakteriozne mrljavosti plodova lubenice uočena je šezdesetih godina prošlog
veka na Floridi, ali pažnju istraživača privukla je tek nakon dvadeset godina, kada su usled
iznenadne pojave epidemije nastali ogromni gubici u proizvodnji lubenice u nekoliko saveznih
američkih država. Na teritoriji naše zemlje prouzrokovač ovog oboljenja, karantinska vrsta A.
citrulli, po prvi put dospeva putem zaraženog semena 2014. godine.
Nakon ovog nalaza, patogen je sporadično detektovan na različitim lokalitetima i u
narednim sezonama. S obzirom na gubitke u proizvodnji koji mogu nastati usled pojave ovog
oboljenja, karantinskog statusa koji i dalje ima na teritoriji naše zemlje, nastavljena su dalja
proučavanja izolovanih sojeva. Pored realizovanih istraživanja o diverzitetu sojeva na
biohemijsko-fiziološkom nivou, u okviru ove studije proučavane su genetičke osobine
izolovanih sojeva.
Na osnovu rezultata prethodnih istraživanja, sojevi A. citrulli se mogu razdvojiti u dve
genetički različite grupe. Ova podela se dovodi u vezu sa krugom domaćina, pa tako grupi I
pripadaju sojevi koji su izolovani i ispoljavaju visoku virulentnost prema različitim
domaćinima familije Cucurbitaceae, dok su sojevi II grupe izolovani iz lubenice, prema kojoj
su naročito agresivni. Međutim, intenzivna proučavanja sojeva A. citrulli u svetu poslednjih
nekoliko godina, pokazala su da se ne može postaviti jasna granica među sojevima samo na
osnovu domaćina i da su rezultati u slučaju prirodne i veštački izazvane infekcije izrazito
varijabilni. Tako je pokazano da u uslovima veštačke inokulacije nije preterano izražena
specifičnost sojeva prema domaćinu, za razliku od infekcije nastale prirodnim putem u polju.
Stoga je u okviru naše studije proučena genetička pripadnost različitim grupama 41 soja A.
citrulli poreklom iz Srbije, izolovanih u periodu 2014-2018. godine. Izvedena je PCR reakcija
prema protokolu Živanović and Walcott (2016), gde se primenom posebno dizajniranih
prajmera, sojevi razvrstavaju u navedene dve grupe. Takođe, u cilju potvrde dobijenih
rezultata, primenjen je i duplex PCR, prema protokolu Zhao et al. (2020). Rezultati testiranja
su pokazali da sojevi izolovani u Srbiji predstavljaju homogenu populaciju i pripradaju II grupi
sojeva patogena lubenice. Ovakav rezultat se mogao očekivati imajući u vidu da su svi
proučavani sojevi izolovani iz biljaka lubenice.
Činjenica da proučavani sojevi pripadaju grupi II ukazuje da će lubenica i dalje biti
najugroženiji domaćin ove bakterije u nas, dok su manje verovatne štete u proizvodnji drugih
vrsta familije Cucurbitaceae.
PB  - Društvo za zaštitu bilja
C3  - XV SAVETOVANJE O ZAŠTITI BILJA, Zlatibor, 22-25. februar 2021. godine
T1  - HETEROGENOST SOJEVA Acidovorax citrulli POREKLOM IZ SRBIJE
EP  - 63
SP  - 62
ER  - 

@conference{
author = "Zlatković, Nevena and Gašić, Katarina and Prokić, Anđelka and Kuzmanović, Nemanja and Ivanović, Milan and Živković, Svetlana and Obradović, Aleksa",
year = "2021",
abstract = "Pojava bakteriozne mrljavosti plodova lubenice uočena je šezdesetih godina prošlog
veka na Floridi, ali pažnju istraživača privukla je tek nakon dvadeset godina, kada su usled
iznenadne pojave epidemije nastali ogromni gubici u proizvodnji lubenice u nekoliko saveznih
američkih država. Na teritoriji naše zemlje prouzrokovač ovog oboljenja, karantinska vrsta A.
citrulli, po prvi put dospeva putem zaraženog semena 2014. godine.
Nakon ovog nalaza, patogen je sporadično detektovan na različitim lokalitetima i u
narednim sezonama. S obzirom na gubitke u proizvodnji koji mogu nastati usled pojave ovog
oboljenja, karantinskog statusa koji i dalje ima na teritoriji naše zemlje, nastavljena su dalja
proučavanja izolovanih sojeva. Pored realizovanih istraživanja o diverzitetu sojeva na
biohemijsko-fiziološkom nivou, u okviru ove studije proučavane su genetičke osobine
izolovanih sojeva.
Na osnovu rezultata prethodnih istraživanja, sojevi A. citrulli se mogu razdvojiti u dve
genetički različite grupe. Ova podela se dovodi u vezu sa krugom domaćina, pa tako grupi I
pripadaju sojevi koji su izolovani i ispoljavaju visoku virulentnost prema različitim
domaćinima familije Cucurbitaceae, dok su sojevi II grupe izolovani iz lubenice, prema kojoj
su naročito agresivni. Međutim, intenzivna proučavanja sojeva A. citrulli u svetu poslednjih
nekoliko godina, pokazala su da se ne može postaviti jasna granica među sojevima samo na
osnovu domaćina i da su rezultati u slučaju prirodne i veštački izazvane infekcije izrazito
varijabilni. Tako je pokazano da u uslovima veštačke inokulacije nije preterano izražena
specifičnost sojeva prema domaćinu, za razliku od infekcije nastale prirodnim putem u polju.
Stoga je u okviru naše studije proučena genetička pripadnost različitim grupama 41 soja A.
citrulli poreklom iz Srbije, izolovanih u periodu 2014-2018. godine. Izvedena je PCR reakcija
prema protokolu Živanović and Walcott (2016), gde se primenom posebno dizajniranih
prajmera, sojevi razvrstavaju u navedene dve grupe. Takođe, u cilju potvrde dobijenih
rezultata, primenjen je i duplex PCR, prema protokolu Zhao et al. (2020). Rezultati testiranja
su pokazali da sojevi izolovani u Srbiji predstavljaju homogenu populaciju i pripradaju II grupi
sojeva patogena lubenice. Ovakav rezultat se mogao očekivati imajući u vidu da su svi
proučavani sojevi izolovani iz biljaka lubenice.
Činjenica da proučavani sojevi pripadaju grupi II ukazuje da će lubenica i dalje biti
najugroženiji domaćin ove bakterije u nas, dok su manje verovatne štete u proizvodnji drugih
vrsta familije Cucurbitaceae.",
publisher = "Društvo za zaštitu bilja",
journal = "XV SAVETOVANJE O ZAŠTITI BILJA, Zlatibor, 22-25. februar 2021. godine",
title = "HETEROGENOST SOJEVA Acidovorax citrulli POREKLOM IZ SRBIJE",
pages = "63-62"
}

Zlatković, N., Gašić, K., Prokić, A., Kuzmanović, N., Ivanović, M., Živković, S.,& Obradović, A.. (2021). HETEROGENOST SOJEVA Acidovorax citrulli POREKLOM IZ SRBIJE. in XV SAVETOVANJE O ZAŠTITI BILJA, Zlatibor, 22-25. februar 2021. godine
Društvo za zaštitu bilja., 62-63.

Zlatković N, Gašić K, Prokić A, Kuzmanović N, Ivanović M, Živković S, Obradović A. HETEROGENOST SOJEVA Acidovorax citrulli POREKLOM IZ SRBIJE. in XV SAVETOVANJE O ZAŠTITI BILJA, Zlatibor, 22-25. februar 2021. godine. 2021;:62-63..

Zlatković, Nevena, Gašić, Katarina, Prokić, Anđelka, Kuzmanović, Nemanja, Ivanović, Milan, Živković, Svetlana, Obradović, Aleksa, "HETEROGENOST SOJEVA Acidovorax citrulli POREKLOM IZ SRBIJE" in XV SAVETOVANJE O ZAŠTITI BILJA, Zlatibor, 22-25. februar 2021. godine (2021):62-63.

TY  - JOUR
AU  - Ristić, Lela
AU  - Vučurović, Ivan
AU  - Vučurović, Ana
AU  - Živković, Svetlana
AU  - Gašić, Katarina
AU  - Kuzmanović, Slobodan
AU  - Starović, Mira
PY  - 2021
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/716
AB  - The distribution and frequency of potato leaf roll virus in the four most important potato growing regions in Serbia were studied during the seven years (2012–18). One hundred randomly collected potato tubers were sampled from each seed lot. The young leaves that developed in three weeks were sampled and tested to record infection rate. The presence of potato leaf roll virus was detected by double-antibody sandwich enzyme-linked immunosorbent assay and disease incidence was calculated using standard formula. The obtained result showed that the highest prevalence of potato leaf roll virus was detected from seed potato samples originated from the Raški region during 2018 (20.7%), while in the Moravički region, only 2.3–11.1% of the potato leaf roll virus was detected every year. The average annual potato leaf roll virus infection was the highest in 2012 (8.4%) and 2018 (8.0%). For further confirmation of potato leaf roll virus infection, reverse transcription (RT)-PCR was performed using specific primers PLRVCPvEcoRI /PLRVCPcNcoI, designed to amplify a 650 bp fragment of the full-length coat protein gene. The PCR products derived from 26 isolates were directly sequenced using the same primer pair as in RT-PCR. The coat protein sequence analysis revealed that the Serbian potato leaf roll virus isolates showed very low nucleotide diversity (95.9–100%). They shared the highest nt identities of 98.08–99.36% with the sequences of potato leaf roll virus isolates deposited in the GenBank from other parts of the world. Phylogenetic analysis and the haplotype network of the coat protein gene sequences showed that the Serbian potato leaf roll virus isolates could be classified in two different groups indicating two possible introductions of the virus to Serbia. The results of this study confirmed the importance of potato leaf roll virus in seed potato production in Serbia. Additionally, this research highlights the need for a continuous monitoring of the potato seeds produced in Serbia as well as imported seeds for the presence of potato leaf roll virus.
PB  - Springer Verlag
T2  - European Journal of Plant Pathology
T1  - Incidence and molecular characterization of potato leaf roll virus in seed potato production in Serbia
EP  - 324
IS  - 2
SP  - 315
VL  - 160
DO  - 10.1007/s10658-021-02243-6
ER  - 

@article{
author = "Ristić, Lela and Vučurović, Ivan and Vučurović, Ana and Živković, Svetlana and Gašić, Katarina and Kuzmanović, Slobodan and Starović, Mira",
year = "2021",
abstract = "The distribution and frequency of potato leaf roll virus in the four most important potato growing regions in Serbia were studied during the seven years (2012–18). One hundred randomly collected potato tubers were sampled from each seed lot. The young leaves that developed in three weeks were sampled and tested to record infection rate. The presence of potato leaf roll virus was detected by double-antibody sandwich enzyme-linked immunosorbent assay and disease incidence was calculated using standard formula. The obtained result showed that the highest prevalence of potato leaf roll virus was detected from seed potato samples originated from the Raški region during 2018 (20.7%), while in the Moravički region, only 2.3–11.1% of the potato leaf roll virus was detected every year. The average annual potato leaf roll virus infection was the highest in 2012 (8.4%) and 2018 (8.0%). For further confirmation of potato leaf roll virus infection, reverse transcription (RT)-PCR was performed using specific primers PLRVCPvEcoRI /PLRVCPcNcoI, designed to amplify a 650 bp fragment of the full-length coat protein gene. The PCR products derived from 26 isolates were directly sequenced using the same primer pair as in RT-PCR. The coat protein sequence analysis revealed that the Serbian potato leaf roll virus isolates showed very low nucleotide diversity (95.9–100%). They shared the highest nt identities of 98.08–99.36% with the sequences of potato leaf roll virus isolates deposited in the GenBank from other parts of the world. Phylogenetic analysis and the haplotype network of the coat protein gene sequences showed that the Serbian potato leaf roll virus isolates could be classified in two different groups indicating two possible introductions of the virus to Serbia. The results of this study confirmed the importance of potato leaf roll virus in seed potato production in Serbia. Additionally, this research highlights the need for a continuous monitoring of the potato seeds produced in Serbia as well as imported seeds for the presence of potato leaf roll virus.",
publisher = "Springer Verlag",
journal = "European Journal of Plant Pathology",
title = "Incidence and molecular characterization of potato leaf roll virus in seed potato production in Serbia",
pages = "324-315",
number = "2",
volume = "160",
doi = "10.1007/s10658-021-02243-6"
}

Ristić, L., Vučurović, I., Vučurović, A., Živković, S., Gašić, K., Kuzmanović, S.,& Starović, M.. (2021). Incidence and molecular characterization of potato leaf roll virus in seed potato production in Serbia. in European Journal of Plant Pathology
Springer Verlag., 160(2), 315-324.
https://doi.org/10.1007/s10658-021-02243-6

Ristić L, Vučurović I, Vučurović A, Živković S, Gašić K, Kuzmanović S, Starović M. Incidence and molecular characterization of potato leaf roll virus in seed potato production in Serbia. in European Journal of Plant Pathology. 2021;160(2):315-324.
doi:10.1007/s10658-021-02243-6 .

Ristić, Lela, Vučurović, Ivan, Vučurović, Ana, Živković, Svetlana, Gašić, Katarina, Kuzmanović, Slobodan, Starović, Mira, "Incidence and molecular characterization of potato leaf roll virus in seed potato production in Serbia" in European Journal of Plant Pathology, 160, no. 2 (2021):315-324,
https://doi.org/10.1007/s10658-021-02243-6 . .

TY  - JOUR
AU  - Stošić, Stefan
AU  - Ristić, Danijela
AU  - Živković, Svetlana
PY  - 2021
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/620
AB  - Mandarin fruits are one of the most popular among the Citrus genus. They are consumed because of their nutritional and health benefits, as well as pleasant taste and smell. This paper describes the identification and characterization of Penicillium expansum, isolated from molded mandarin fruits. The obtained isolates were cultivated on five media [Czapek Yeast Autolysate agar (CYA), MEA (Malt extract agar), Creatine sucrose agar (CREA), Yeast extract sucrose agar (YES), and Oatmeal agar (OA)] and at five different incubation temperatures (5, 15, 25, 30, and 37 °C). Isolates were sequenced for two molecular loci: internal transcribed spacer and beta-tubulin. Based on the results from morphological, physiological, molecular, and phylogenetic analyses, the recovered isolates were identified as P. expansum. The isolated species was confirmed as pathogenic to mandarin fruits in a pathogenicity test. To the best of our knowledge, this is the first report of P. expansum as a postharvest pathogen of mandarin fruit in Serbia.
PB  - Matica Srpska
T2  - Zbornik Matice srpske za prirodne nauke
T1  - Postharvest decay of mandarin fruit in Serbia caused by Penicillium expansum
EP  - 44
SP  - 29
VL  - 140
DO  - 10.2298/ZMSPN2140029S
ER  - 

@article{
author = "Stošić, Stefan and Ristić, Danijela and Živković, Svetlana",
year = "2021",
abstract = "Mandarin fruits are one of the most popular among the Citrus genus. They are consumed because of their nutritional and health benefits, as well as pleasant taste and smell. This paper describes the identification and characterization of Penicillium expansum, isolated from molded mandarin fruits. The obtained isolates were cultivated on five media [Czapek Yeast Autolysate agar (CYA), MEA (Malt extract agar), Creatine sucrose agar (CREA), Yeast extract sucrose agar (YES), and Oatmeal agar (OA)] and at five different incubation temperatures (5, 15, 25, 30, and 37 °C). Isolates were sequenced for two molecular loci: internal transcribed spacer and beta-tubulin. Based on the results from morphological, physiological, molecular, and phylogenetic analyses, the recovered isolates were identified as P. expansum. The isolated species was confirmed as pathogenic to mandarin fruits in a pathogenicity test. To the best of our knowledge, this is the first report of P. expansum as a postharvest pathogen of mandarin fruit in Serbia.",
publisher = "Matica Srpska",
journal = "Zbornik Matice srpske za prirodne nauke",
title = "Postharvest decay of mandarin fruit in Serbia caused by Penicillium expansum",
pages = "44-29",
volume = "140",
doi = "10.2298/ZMSPN2140029S"
}

Stošić, S., Ristić, D.,& Živković, S.. (2021). Postharvest decay of mandarin fruit in Serbia caused by Penicillium expansum. in Zbornik Matice srpske za prirodne nauke
Matica Srpska., 140, 29-44.
https://doi.org/10.2298/ZMSPN2140029S

Stošić S, Ristić D, Živković S. Postharvest decay of mandarin fruit in Serbia caused by Penicillium expansum. in Zbornik Matice srpske za prirodne nauke. 2021;140:29-44.
doi:10.2298/ZMSPN2140029S .

Stošić, Stefan, Ristić, Danijela, Živković, Svetlana, "Postharvest decay of mandarin fruit in Serbia caused by Penicillium expansum" in Zbornik Matice srpske za prirodne nauke, 140 (2021):29-44,
https://doi.org/10.2298/ZMSPN2140029S . .

TY  - JOUR
AU  - Stošić, Stefan
AU  - Ristić, Lela
AU  - Gašić, Katarina
AU  - Starović, Mira
AU  - Ljaljevic Grbic, Milica
AU  - Vukojevic, Jelena
AU  - Živković, Svetlana
PY  - 2020
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/717
AB  - Talaromyces minioluteus is one of the important species of genus Talaromyces, which has cosmopolitan distribution and is encountered on a wide range of different habitats. This species has not been considered as an important plant pathogen, even though it has been isolated from various plant hosts. Fruits and vegetables with Penicillium-like mold symptoms were collected from 2015 to 2017 from markets in Serbia. Isolates originating from quince, tomato, and orange fruits, onion bulbs, and potato tubers were identified and characterized on a morphological, physiological, and molecular level. Morphological and physiological examination included observing micromorphology, testing growth on six different media and at five different temperatures, and production of three enzymes. Molecular identification and characterization were performed using four molecular markers: internal transcribed spacer, β-tubulin, calmodulin, and DNA-dependent RNA polymerase II second largest subunit. The results of morphological and molecular analyses were in agreement, and they proved that the obtained isolates are T. minioluteus. In the pathogenicity assay, T. minioluteus was confirmed as a pathogen of all species tested with the exception of potato tubers. This is the first report of T. minioluteus as a postharvest plant pathogen on quince, tomato, and orange fruit and onion bulbs. Also, this is the first record of T. minioluteus in Serbia.
PB  - The American Phytopathological Society
T2  - Plant Disease
T1  - Talaromyces minioluteus: New Postharvest Fungal Pathogen in Serbia
EP  - 667
IS  - 3
SP  - 656
VL  - 104
DO  - 10.1094/PDIS-08-19-1806-RE
ER  - 

@article{
author = "Stošić, Stefan and Ristić, Lela and Gašić, Katarina and Starović, Mira and Ljaljevic Grbic, Milica and Vukojevic, Jelena and Živković, Svetlana",
year = "2020",
abstract = "Talaromyces minioluteus is one of the important species of genus Talaromyces, which has cosmopolitan distribution and is encountered on a wide range of different habitats. This species has not been considered as an important plant pathogen, even though it has been isolated from various plant hosts. Fruits and vegetables with Penicillium-like mold symptoms were collected from 2015 to 2017 from markets in Serbia. Isolates originating from quince, tomato, and orange fruits, onion bulbs, and potato tubers were identified and characterized on a morphological, physiological, and molecular level. Morphological and physiological examination included observing micromorphology, testing growth on six different media and at five different temperatures, and production of three enzymes. Molecular identification and characterization were performed using four molecular markers: internal transcribed spacer, β-tubulin, calmodulin, and DNA-dependent RNA polymerase II second largest subunit. The results of morphological and molecular analyses were in agreement, and they proved that the obtained isolates are T. minioluteus. In the pathogenicity assay, T. minioluteus was confirmed as a pathogen of all species tested with the exception of potato tubers. This is the first report of T. minioluteus as a postharvest plant pathogen on quince, tomato, and orange fruit and onion bulbs. Also, this is the first record of T. minioluteus in Serbia.",
publisher = "The American Phytopathological Society",
journal = "Plant Disease",
title = "Talaromyces minioluteus: New Postharvest Fungal Pathogen in Serbia",
pages = "667-656",
number = "3",
volume = "104",
doi = "10.1094/PDIS-08-19-1806-RE"
}

Stošić, S., Ristić, L., Gašić, K., Starović, M., Ljaljevic Grbic, M., Vukojevic, J.,& Živković, S.. (2020). Talaromyces minioluteus: New Postharvest Fungal Pathogen in Serbia. in Plant Disease
The American Phytopathological Society., 104(3), 656-667.
https://doi.org/10.1094/PDIS-08-19-1806-RE

Stošić S, Ristić L, Gašić K, Starović M, Ljaljevic Grbic M, Vukojevic J, Živković S. Talaromyces minioluteus: New Postharvest Fungal Pathogen in Serbia. in Plant Disease. 2020;104(3):656-667.
doi:10.1094/PDIS-08-19-1806-RE .

Stošić, Stefan, Ristić, Lela, Gašić, Katarina, Starović, Mira, Ljaljevic Grbic, Milica, Vukojevic, Jelena, Živković, Svetlana, "Talaromyces minioluteus: New Postharvest Fungal Pathogen in Serbia" in Plant Disease, 104, no. 3 (2020):656-667,
https://doi.org/10.1094/PDIS-08-19-1806-RE . .

TY  - CONF
AU  - Aleksic, Goran
AU  - Starović, Mira
AU  - Zivkovic, Svetlana
AU  - Kuzmanović, Slobodan
PY  - 2019
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/1010
AB  - Према подацима ФАО светска производња пестици- да, од половине прошлог века, увећава се по стопи од око 11% годишње, од 0,2 милиона тона 50-их година прошлог века до више од 5 милиона тона до 2000. године. Овако велика производња и промет пестицида у најразвијени- јим деловима света последица је интензивирања пољопривредне производње и све већих потреба за храном. Свакако да је овај ниво потрошње пестици- да и резултат деловања биоагенаса у биљној производњи, односно све веће опасности од потенцијалних штета које могу изазвати проузроковачи биљ- них болести и штеточине пољопривредног биља. Међутим, често је употреба пестицида против штетних организама у биљној производњи прекомерна и неадекватна, па су последице које због тога настају, са здравственог, еколо- шког и економског аспекта, веома значајне. Због свега поменутог, прогноза појаве штетних организама у биљној производњи, као један од најважнијих сегмената заштите биља, још више добија на значају јер доприноси правовре- меној, адекватној и рационалној употреби хемијских средстава. Значајан део активности у овој области је у функцији проучавања биологије и екологије штетних организама и све то у циљу њиховог успешног, ефикасног и, што је могуће рационалнијег, сузбијања.
PB  - SANU
C3  - Коришћење пестицида у биљној производњи и заштита животне средине
T1  - Značaj prognozno-izveštajne službe u suzbijanju štetnih organizama u poljoprivredi
EP  - 286
SP  - 269
ER  - 

@conference{
author = "Aleksic, Goran and Starović, Mira and Zivkovic, Svetlana and Kuzmanović, Slobodan",
year = "2019",
abstract = "Према подацима ФАО светска производња пестици- да, од половине прошлог века, увећава се по стопи од око 11% годишње, од 0,2 милиона тона 50-их година прошлог века до више од 5 милиона тона до 2000. године. Овако велика производња и промет пестицида у најразвијени- јим деловима света последица је интензивирања пољопривредне производње и све већих потреба за храном. Свакако да је овај ниво потрошње пестици- да и резултат деловања биоагенаса у биљној производњи, односно све веће опасности од потенцијалних штета које могу изазвати проузроковачи биљ- них болести и штеточине пољопривредног биља. Међутим, често је употреба пестицида против штетних организама у биљној производњи прекомерна и неадекватна, па су последице које због тога настају, са здравственог, еколо- шког и економског аспекта, веома значајне. Због свега поменутог, прогноза појаве штетних организама у биљној производњи, као један од најважнијих сегмената заштите биља, још више добија на значају јер доприноси правовре- меној, адекватној и рационалној употреби хемијских средстава. Значајан део активности у овој области је у функцији проучавања биологије и екологије штетних организама и све то у циљу њиховог успешног, ефикасног и, што је могуће рационалнијег, сузбијања.",
publisher = "SANU",
journal = "Коришћење пестицида у биљној производњи и заштита животне средине",
title = "Značaj prognozno-izveštajne službe u suzbijanju štetnih organizama u poljoprivredi",
pages = "286-269"
}

Aleksic, G., Starović, M., Zivkovic, S.,& Kuzmanović, S.. (2019). Značaj prognozno-izveštajne službe u suzbijanju štetnih organizama u poljoprivredi. in Коришћење пестицида у биљној производњи и заштита животне средине
SANU., 269-286.

Aleksic G, Starović M, Zivkovic S, Kuzmanović S. Značaj prognozno-izveštajne službe u suzbijanju štetnih organizama u poljoprivredi. in Коришћење пестицида у биљној производњи и заштита животне средине. 2019;:269-286..

Aleksic, Goran, Starović, Mira, Zivkovic, Svetlana, Kuzmanović, Slobodan, "Značaj prognozno-izveštajne službe u suzbijanju štetnih organizama u poljoprivredi" in Коришћење пестицида у биљној производњи и заштита животне средине (2019):269-286.

TY  - JOUR
AU  - Zivkovic, Svetlana
AU  - Stošić, Stefan
AU  - Ristić, Danijela
AU  - Vučurović, Ivan
AU  - Stevanović, Miloš
PY  - 2019
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/917
AB  - Lactobacillus plantarum, one of the most widespread lactic acid bacte¬ria, exert a strong antagonistic activity against many microorganisms. The present study was conducted to determine in vitro and in situ antagonistic potential of L. plantarum (DSM 20174) for control postharvest decay caused by phytopathogenic fungi: Aspergillus flavus, Colletotrichum acutatum, Colletotrichum gloeosporioides, and Fusarium avenaceum. The results obtained in in vitro assays showed that L. plantarum had a stronger inhibitory effect on spore germination than on mycelia growth of all tested fungi. After 3 days of incubation, the diameter of inhibition zones ranged from 11.67 mm for C. gloeosporioides to 14.67 mm for C. acutatum. The bacterial suspension of L. plantarum significantly inhibited conidial germination of all postharvest pathogens (89.62-97.61%). In situ assays showed that treatment with L. plantarum efficiently inhibited necrosis ranging from 42.54% for C. acutatum to 54.47% for A. flavus. The disease incidence in L. plantarum treated fruits was statistically significantly lower than in the positive control for all fungi tested (P<0.05). The presented data demonstrate the antagonistic potential of L. plantarum (DSM 20174) and indicate the possibility of using this bacterial strain as a biological agent to control postharvest fungal pathogens.
PB  - Matica srpska
T2  - Zbornik Matice srpske za prirodne nauke
T1  - Antagonistic potential of lactobacillus plantarum against some postharvest pathogenic fungi
EP  - 88
SP  - 79
VL  - 136
DO  - 10.2298/zmspn1936079z
ER  - 

@article{
author = "Zivkovic, Svetlana and Stošić, Stefan and Ristić, Danijela and Vučurović, Ivan and Stevanović, Miloš",
year = "2019",
abstract = "Lactobacillus plantarum, one of the most widespread lactic acid bacte¬ria, exert a strong antagonistic activity against many microorganisms. The present study was conducted to determine in vitro and in situ antagonistic potential of L. plantarum (DSM 20174) for control postharvest decay caused by phytopathogenic fungi: Aspergillus flavus, Colletotrichum acutatum, Colletotrichum gloeosporioides, and Fusarium avenaceum. The results obtained in in vitro assays showed that L. plantarum had a stronger inhibitory effect on spore germination than on mycelia growth of all tested fungi. After 3 days of incubation, the diameter of inhibition zones ranged from 11.67 mm for C. gloeosporioides to 14.67 mm for C. acutatum. The bacterial suspension of L. plantarum significantly inhibited conidial germination of all postharvest pathogens (89.62-97.61%). In situ assays showed that treatment with L. plantarum efficiently inhibited necrosis ranging from 42.54% for C. acutatum to 54.47% for A. flavus. The disease incidence in L. plantarum treated fruits was statistically significantly lower than in the positive control for all fungi tested (P<0.05). The presented data demonstrate the antagonistic potential of L. plantarum (DSM 20174) and indicate the possibility of using this bacterial strain as a biological agent to control postharvest fungal pathogens.",
publisher = "Matica srpska",
journal = "Zbornik Matice srpske za prirodne nauke",
title = "Antagonistic potential of lactobacillus plantarum against some postharvest pathogenic fungi",
pages = "88-79",
volume = "136",
doi = "10.2298/zmspn1936079z"
}

Zivkovic, S., Stošić, S., Ristić, D., Vučurović, I.,& Stevanović, M.. (2019). Antagonistic potential of lactobacillus plantarum against some postharvest pathogenic fungi. in Zbornik Matice srpske za prirodne nauke
Matica srpska., 136, 79-88.
https://doi.org/10.2298/zmspn1936079z

Zivkovic S, Stošić S, Ristić D, Vučurović I, Stevanović M. Antagonistic potential of lactobacillus plantarum against some postharvest pathogenic fungi. in Zbornik Matice srpske za prirodne nauke. 2019;136:79-88.
doi:10.2298/zmspn1936079z .

Zivkovic, Svetlana, Stošić, Stefan, Ristić, Danijela, Vučurović, Ivan, Stevanović, Miloš, "Antagonistic potential of lactobacillus plantarum against some postharvest pathogenic fungi" in Zbornik Matice srpske za prirodne nauke, 136 (2019):79-88,
https://doi.org/10.2298/zmspn1936079z . .

TY  - JOUR
AU  - Aleksić, Goran
AU  - Milićević, Zoran
AU  - Kuzmanović, Slobodan
AU  - Starović, Mira
AU  - Stevanović, Miloš
AU  - Delibašić, Goran
AU  - Živković, Svetlana
PY  - 2019
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5229
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/599
AB  - The control of Plasmopara viticola and Botrytis cinerea, two of the most dangerous pathogens on grapevine, requires frequent treatments with chemicals based on weather conditions. Numerous applications of fungicides have resulted in developing fungicide resistance. Active ingredients based on copper have been used very successfully for a long time to protect grapevines against these pathogens. Application of a copper citrate formulation with high degree dissociation at a very low concentration (1%) was evaluated in field trials. The efficacy of two concentrations of copper citrate, 0.5 and 1.0%, were tested against P. viticola on grapevine in three locations, and against B. cinerea in two locations during 2018. Our results demonstrated that the concentration of 1.0% copper citrate was highly effective against P. viticola (87.4%) and B. cinerea (63.7%), compared to standard treatment (90.6 and 53.1%), under a high level of infection.
AB  - Zaštita vinove loze od prouzrokovača plamenjače vinove loze - Plasmopara viticola i sive truleži - Botrytis cinerea, je vrlo kompleksna i zahteva primenu većeg broja hemijskih tretmana, u skladu sa vremenskim uslovima. Učestala primena fungicida uslovljavala je pojavu rezistentnih izolata patogena na fungicide. Različite forme bakarnih jedinjenja primenjuju se u zaštiti vinove loze dugi niz godina prilično uspešno. Primena bakar-citrata - formulacije sa visokim stepenom disocijacije u niskoj koncentraciji (1,0%) ispitivana je u poljskim uslovima. Efikasnost dve koncentracije bakar-citrata - 0.5 i 1.0% je testirana u suzbijanju P. viticola i B. cinerea na vinovoj lozi na tri (dva) lokaliteta, tokom 2018 godine. Naši rezultati pokazuju da je ispitivana koncentracija od 1,0% bakar-citrata ispoljila zadovoljavajući efekat na P. viticola (87,4%) i B. cinerea (63,7%) u odnosu na primenjene standarde (90,6% i 53,1%) u uslovima visokih zaraza.
PB  - Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd
T2  - Pesticidi i fitomedicina
T1  - Efficacy of copper citrate in grapevine disease control
T1  - Efikasnost bakar-citrata u zaštiti vinove loze od bolesti
EP  - 109
IS  - 2
SP  - 103
VL  - 34
ER  - 

@article{
author = "Aleksić, Goran and Milićević, Zoran and Kuzmanović, Slobodan and Starović, Mira and Stevanović, Miloš and Delibašić, Goran and Živković, Svetlana",
year = "2019",
abstract = "The control of Plasmopara viticola and Botrytis cinerea, two of the most dangerous pathogens on grapevine, requires frequent treatments with chemicals based on weather conditions. Numerous applications of fungicides have resulted in developing fungicide resistance. Active ingredients based on copper have been used very successfully for a long time to protect grapevines against these pathogens. Application of a copper citrate formulation with high degree dissociation at a very low concentration (1%) was evaluated in field trials. The efficacy of two concentrations of copper citrate, 0.5 and 1.0%, were tested against P. viticola on grapevine in three locations, and against B. cinerea in two locations during 2018. Our results demonstrated that the concentration of 1.0% copper citrate was highly effective against P. viticola (87.4%) and B. cinerea (63.7%), compared to standard treatment (90.6 and 53.1%), under a high level of infection., Zaštita vinove loze od prouzrokovača plamenjače vinove loze - Plasmopara viticola i sive truleži - Botrytis cinerea, je vrlo kompleksna i zahteva primenu većeg broja hemijskih tretmana, u skladu sa vremenskim uslovima. Učestala primena fungicida uslovljavala je pojavu rezistentnih izolata patogena na fungicide. Različite forme bakarnih jedinjenja primenjuju se u zaštiti vinove loze dugi niz godina prilično uspešno. Primena bakar-citrata - formulacije sa visokim stepenom disocijacije u niskoj koncentraciji (1,0%) ispitivana je u poljskim uslovima. Efikasnost dve koncentracije bakar-citrata - 0.5 i 1.0% je testirana u suzbijanju P. viticola i B. cinerea na vinovoj lozi na tri (dva) lokaliteta, tokom 2018 godine. Naši rezultati pokazuju da je ispitivana koncentracija od 1,0% bakar-citrata ispoljila zadovoljavajući efekat na P. viticola (87,4%) i B. cinerea (63,7%) u odnosu na primenjene standarde (90,6% i 53,1%) u uslovima visokih zaraza.",
publisher = "Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd",
journal = "Pesticidi i fitomedicina",
title = "Efficacy of copper citrate in grapevine disease control, Efikasnost bakar-citrata u zaštiti vinove loze od bolesti",
pages = "109-103",
number = "2",
volume = "34"
}

Aleksić, G., Milićević, Z., Kuzmanović, S., Starović, M., Stevanović, M., Delibašić, G.,& Živković, S.. (2019). Efficacy of copper citrate in grapevine disease control. in Pesticidi i fitomedicina
Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd., 34(2), 103-109.

Aleksić G, Milićević Z, Kuzmanović S, Starović M, Stevanović M, Delibašić G, Živković S. Efficacy of copper citrate in grapevine disease control. in Pesticidi i fitomedicina. 2019;34(2):103-109..

Aleksić, Goran, Milićević, Zoran, Kuzmanović, Slobodan, Starović, Mira, Stevanović, Miloš, Delibašić, Goran, Živković, Svetlana, "Efficacy of copper citrate in grapevine disease control" in Pesticidi i fitomedicina, 34, no. 2 (2019):103-109.

TY  - JOUR
AU  - Stevanović, Miloš
AU  - Ristić, Danijela
AU  - Živković, Svetlana
AU  - Aleksić, Goran
AU  - Stanković, Ivana
AU  - Krstić, Branka
AU  - Bulajić, Aleksandra
PY  - 2019
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/570
AB  - Blackberry cane diseases with the symptoms of necrosis, canker, and wilting are caused by several fungi worldwide. Surveys conducted from 2013 to 2016 in Serbia revealed the occurrence of Gnomoniopsis idaeicola, the causal agent of cane canker and wilting, which was found to be distributed in almost half of the surveyed orchards, in three blackberry cultivars, and with disease incidence of up to 80%. Wide distribution and high disease incidence suggest that G. idaeicola has been present in Serbia for some time. Out of 427 samples, a total of 65 G. idaeicola isolates were obtained (isolation rate of 34.19%). Reference isolates, originating from different localities, were conventionally and molecularly identified and characterized. G. idaeicola was detected in single and mixed infections with fungi from genera Paraconiothyrium, Colletotrichum, Diaporthe, Botryosphaeria, Botrytis, Septoria, Neofusicoccum, and Discostroma, and no diagnostically specific symptoms could be related directly to the G. idaeicola infection. In orchards solely infected with G. idaeicola, blackberry plant mortality was up to 40%, and yield loses were estimated at 50%. G. idaeicola isolates included in this study demonstrated intraspecies diversity in morphological, biological, pathogenic, and molecular features, which indicates that population in Serbia may be of different origin. This is the first record of a massive outbreak of G. idaeicola infection, illustrating its capability of harmful influence on blackberry production. This study represents the initial step in studying G. idaeicola as a new blackberry pathogen in Serbia, aiming at developing efficient control measures.
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - Characterization of Gnomoniopsis idaeicola, the Causal Agent of Canker and Wilting of Blackberry in Serbia
EP  - 258
IS  - 2
SP  - 249
VL  - 103
DO  - 10.1094/PDIS-03-18-0516-RE
ER  - 

@article{
author = "Stevanović, Miloš and Ristić, Danijela and Živković, Svetlana and Aleksić, Goran and Stanković, Ivana and Krstić, Branka and Bulajić, Aleksandra",
year = "2019",
abstract = "Blackberry cane diseases with the symptoms of necrosis, canker, and wilting are caused by several fungi worldwide. Surveys conducted from 2013 to 2016 in Serbia revealed the occurrence of Gnomoniopsis idaeicola, the causal agent of cane canker and wilting, which was found to be distributed in almost half of the surveyed orchards, in three blackberry cultivars, and with disease incidence of up to 80%. Wide distribution and high disease incidence suggest that G. idaeicola has been present in Serbia for some time. Out of 427 samples, a total of 65 G. idaeicola isolates were obtained (isolation rate of 34.19%). Reference isolates, originating from different localities, were conventionally and molecularly identified and characterized. G. idaeicola was detected in single and mixed infections with fungi from genera Paraconiothyrium, Colletotrichum, Diaporthe, Botryosphaeria, Botrytis, Septoria, Neofusicoccum, and Discostroma, and no diagnostically specific symptoms could be related directly to the G. idaeicola infection. In orchards solely infected with G. idaeicola, blackberry plant mortality was up to 40%, and yield loses were estimated at 50%. G. idaeicola isolates included in this study demonstrated intraspecies diversity in morphological, biological, pathogenic, and molecular features, which indicates that population in Serbia may be of different origin. This is the first record of a massive outbreak of G. idaeicola infection, illustrating its capability of harmful influence on blackberry production. This study represents the initial step in studying G. idaeicola as a new blackberry pathogen in Serbia, aiming at developing efficient control measures.",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "Characterization of Gnomoniopsis idaeicola, the Causal Agent of Canker and Wilting of Blackberry in Serbia",
pages = "258-249",
number = "2",
volume = "103",
doi = "10.1094/PDIS-03-18-0516-RE"
}

Stevanović, M., Ristić, D., Živković, S., Aleksić, G., Stanković, I., Krstić, B.,& Bulajić, A.. (2019). Characterization of Gnomoniopsis idaeicola, the Causal Agent of Canker and Wilting of Blackberry in Serbia. in Plant Disease
Amer Phytopathological Soc, St Paul., 103(2), 249-258.
https://doi.org/10.1094/PDIS-03-18-0516-RE

Stevanović M, Ristić D, Živković S, Aleksić G, Stanković I, Krstić B, Bulajić A. Characterization of Gnomoniopsis idaeicola, the Causal Agent of Canker and Wilting of Blackberry in Serbia. in Plant Disease. 2019;103(2):249-258.
doi:10.1094/PDIS-03-18-0516-RE .

Stevanović, Miloš, Ristić, Danijela, Živković, Svetlana, Aleksić, Goran, Stanković, Ivana, Krstić, Branka, Bulajić, Aleksandra, "Characterization of Gnomoniopsis idaeicola, the Causal Agent of Canker and Wilting of Blackberry in Serbia" in Plant Disease, 103, no. 2 (2019):249-258,
https://doi.org/10.1094/PDIS-03-18-0516-RE . .

Aleksic, G., Milićević, Z., Kuzmanović, S., Starović, M., Zivkovic, S.,& Gavrilović, V.. (2018). Bakar citrat - nova aktivna materija za defolijaciju voćnog sadnog materijala. in Institut za zaštitu bilja i životnu sredinu.

Aleksic G, Milićević Z, Kuzmanović S, Starović M, Zivkovic S, Gavrilović V. Bakar citrat - nova aktivna materija za defolijaciju voćnog sadnog materijala. in Institut za zaštitu bilja i životnu sredinu. 2018;..

Aleksic, Goran, Milićević, Zoran, Kuzmanović, Slobodan, Starović, Mira, Zivkovic, Svetlana, Gavrilović, Veljko, "Bakar citrat - nova aktivna materija za defolijaciju voćnog sadnog materijala" in Institut za zaštitu bilja i životnu sredinu (2018).

TY  - JOUR
AU  - Gavrilović, Veljko
AU  - Milićević, Zoran
AU  - Aleksic, Goran
AU  - Živković, Svetlana
AU  - Stošić, Stefan
AU  - Starović, Mira
AU  - Kuzmanović, Slobodan
PY  - 2018
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/1015
PB  - Institut PKB Agroekonomik
T2  - Zbornik Instituta PKB agroekonomik
T1  - Primena bakar citrata pri defolijaciji sadnica višnje i breskve
EP  - 49
IS  - 5
SP  - 41
VL  - 24
ER  - 

@article{
author = "Gavrilović, Veljko and Milićević, Zoran and Aleksic, Goran and Živković, Svetlana and Stošić, Stefan and Starović, Mira and Kuzmanović, Slobodan",
year = "2018",
publisher = "Institut PKB Agroekonomik",
journal = "Zbornik Instituta PKB agroekonomik",
title = "Primena bakar citrata pri defolijaciji sadnica višnje i breskve",
pages = "49-41",
number = "5",
volume = "24"
}

Gavrilović, V., Milićević, Z., Aleksic, G., Živković, S., Stošić, S., Starović, M.,& Kuzmanović, S.. (2018). Primena bakar citrata pri defolijaciji sadnica višnje i breskve. in Zbornik Instituta PKB agroekonomik
Institut PKB Agroekonomik., 24(5), 41-49.

Gavrilović V, Milićević Z, Aleksic G, Živković S, Stošić S, Starović M, Kuzmanović S. Primena bakar citrata pri defolijaciji sadnica višnje i breskve. in Zbornik Instituta PKB agroekonomik. 2018;24(5):41-49..

Gavrilović, Veljko, Milićević, Zoran, Aleksic, Goran, Živković, Svetlana, Stošić, Stefan, Starović, Mira, Kuzmanović, Slobodan, "Primena bakar citrata pri defolijaciji sadnica višnje i breskve" in Zbornik Instituta PKB agroekonomik, 24, no. 5 (2018):41-49.

TY  - JOUR
AU  - Živković, Svetlana
AU  - Stevanović, Miloš
AU  - Đurović, Sanja
AU  - Ristić, Danijela
AU  - Stošić, Stefan
PY  - 2018
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/543
AB  - Chitosan and its derivatives have been reported as a promising alternative for control of postharvest fungal pathogens. The objective of this study was to evaluate in vitro and in situ antifungal activity of chitosan against Alternaria alternata and Colletotrichum gloeosporioides isolated from decayed apple fruits. The fungi were tested in vitro using PDA medium with three concentrations of chitosan (1, 2 and 3 mg/ml). Fungal growth of the test pathogens was significantly affected by all chitosan doses (P lt 0.05) after 7 days of incubation at 25°C. Water solution of 3 mg/ml of chitosan inhibited completely the conidial germination of A. alternata and C. gloeosporioides after 18 h incubation at 25°C. The results obtained from biocontrol assay indicate that the inhibition of postharvest decay of A. alternata and C. gloeosporioides was significantly influenced by chitosan concentrations. Disease incidence in chitosan-treated fruit after 7 days incubation at 25°C was significantly lower than in the positive control for both fungi tested (P lt 0.05). A. alternata and C. gloeosporioides used in this study were progressively inhibited in vitro and in situ with increasing concentrations of chitosan from 1 to 3 mg/ml.
AB  - Istraživanja pokazuju da su hitosan i njegovi derivati dobra alternativa u kontroli skladišnih fitopatogenih gljiva. Cilj ovog rada bio je da ispita antifungalnu aktivnost hitosana prema Alternaria alternata i Colletotrichum gloeosporoides, izolovanih sa inficiranih plodova jabuke, u in vitro i in situ uslovima. Rast gljiva je testiran in vitro koristeći PDA podlogu sa različitim koncentracijama hitosana (1, 2 i 3 mg/ml). Porast oba patogena je bio značajno smanjen (P lt 0.05) u svim koncentracijama hitosana nakon 7 dana inkubacije na 25°C. Vodeni rastvor hitosana koncentracije 3 mg/ml je u potpunosti inhibirao klijanje konidija A. alternata i C. gloeosporoides nakon inkubacije od 18 sati na temperaturi od 25°C. Rezultati ogleda in situ ukazuju da inhibicija propadanja plodova inficiranih sa A. alternata i C. gloeosporoides zavisi od koncentracije rastvora hitosana. Pojava bolesti na plodovima jabuka tretiranih rastvorom hitosana nakon 7 dana inkubacije na 25°C bila je značajno smanjena za obe vrste gljiva (P lt 0.05) u odnosu na pozitivnu kontrolu. Rast A. alternata i C. gloeosporoides je bio progresivno inhibiran in vitro i in situ sa povećanjem koncentracije rastvora hitosana, od 1 do 3 mg/ml.
PB  - Institute of Pesticides and Environmental Protection
T2  - Pesticidi i fitomedicina
T1  - Antifungal activity of chitosan against Alternaria alternata and Colletotrichum gloeosporioides
T1  - Antifungalna aktivnost hitosana prema Alternaria alternata i Colletotrichum gloeosporoides
EP  - 204
IS  - 3-4
SP  - 197
VL  - 33
DO  - 10.2298/PIF1804197Z
ER  - 

@article{
author = "Živković, Svetlana and Stevanović, Miloš and Đurović, Sanja and Ristić, Danijela and Stošić, Stefan",
year = "2018",
abstract = "Chitosan and its derivatives have been reported as a promising alternative for control of postharvest fungal pathogens. The objective of this study was to evaluate in vitro and in situ antifungal activity of chitosan against Alternaria alternata and Colletotrichum gloeosporioides isolated from decayed apple fruits. The fungi were tested in vitro using PDA medium with three concentrations of chitosan (1, 2 and 3 mg/ml). Fungal growth of the test pathogens was significantly affected by all chitosan doses (P lt 0.05) after 7 days of incubation at 25°C. Water solution of 3 mg/ml of chitosan inhibited completely the conidial germination of A. alternata and C. gloeosporioides after 18 h incubation at 25°C. The results obtained from biocontrol assay indicate that the inhibition of postharvest decay of A. alternata and C. gloeosporioides was significantly influenced by chitosan concentrations. Disease incidence in chitosan-treated fruit after 7 days incubation at 25°C was significantly lower than in the positive control for both fungi tested (P lt 0.05). A. alternata and C. gloeosporioides used in this study were progressively inhibited in vitro and in situ with increasing concentrations of chitosan from 1 to 3 mg/ml., Istraživanja pokazuju da su hitosan i njegovi derivati dobra alternativa u kontroli skladišnih fitopatogenih gljiva. Cilj ovog rada bio je da ispita antifungalnu aktivnost hitosana prema Alternaria alternata i Colletotrichum gloeosporoides, izolovanih sa inficiranih plodova jabuke, u in vitro i in situ uslovima. Rast gljiva je testiran in vitro koristeći PDA podlogu sa različitim koncentracijama hitosana (1, 2 i 3 mg/ml). Porast oba patogena je bio značajno smanjen (P lt 0.05) u svim koncentracijama hitosana nakon 7 dana inkubacije na 25°C. Vodeni rastvor hitosana koncentracije 3 mg/ml je u potpunosti inhibirao klijanje konidija A. alternata i C. gloeosporoides nakon inkubacije od 18 sati na temperaturi od 25°C. Rezultati ogleda in situ ukazuju da inhibicija propadanja plodova inficiranih sa A. alternata i C. gloeosporoides zavisi od koncentracije rastvora hitosana. Pojava bolesti na plodovima jabuka tretiranih rastvorom hitosana nakon 7 dana inkubacije na 25°C bila je značajno smanjena za obe vrste gljiva (P lt 0.05) u odnosu na pozitivnu kontrolu. Rast A. alternata i C. gloeosporoides je bio progresivno inhibiran in vitro i in situ sa povećanjem koncentracije rastvora hitosana, od 1 do 3 mg/ml.",
publisher = "Institute of Pesticides and Environmental Protection",
journal = "Pesticidi i fitomedicina",
title = "Antifungal activity of chitosan against Alternaria alternata and Colletotrichum gloeosporioides, Antifungalna aktivnost hitosana prema Alternaria alternata i Colletotrichum gloeosporoides",
pages = "204-197",
number = "3-4",
volume = "33",
doi = "10.2298/PIF1804197Z"
}

Živković, S., Stevanović, M., Đurović, S., Ristić, D.,& Stošić, S.. (2018). Antifungal activity of chitosan against Alternaria alternata and Colletotrichum gloeosporioides. in Pesticidi i fitomedicina
Institute of Pesticides and Environmental Protection., 33(3-4), 197-204.
https://doi.org/10.2298/PIF1804197Z

Živković S, Stevanović M, Đurović S, Ristić D, Stošić S. Antifungal activity of chitosan against Alternaria alternata and Colletotrichum gloeosporioides. in Pesticidi i fitomedicina. 2018;33(3-4):197-204.
doi:10.2298/PIF1804197Z .

Živković, Svetlana, Stevanović, Miloš, Đurović, Sanja, Ristić, Danijela, Stošić, Stefan, "Antifungal activity of chitosan against Alternaria alternata and Colletotrichum gloeosporioides" in Pesticidi i fitomedicina, 33, no. 3-4 (2018):197-204,
https://doi.org/10.2298/PIF1804197Z . .

Aleksic, G., Kuzmanović, S., Zivkovic, S., Popović Milovanović, T., Ristić, D., Stevanović, M.,& Borić, B.. (2017). Program prognoze Venturia inaequalis - prouzrokovača čađave krastavosti jabuke. in Institut za zaštitu bilja i životnu sredinu
Institut za zaštitu bilja i životnu sredinu, Beograd..

Aleksic G, Kuzmanović S, Zivkovic S, Popović Milovanović T, Ristić D, Stevanović M, Borić B. Program prognoze Venturia inaequalis - prouzrokovača čađave krastavosti jabuke. in Institut za zaštitu bilja i životnu sredinu. 2017;..

Aleksic, Goran, Kuzmanović, Slobodan, Zivkovic, Svetlana, Popović Milovanović, Tatjana, Ristić, Danijela, Stevanović, Miloš, Borić, Borislav, "Program prognoze Venturia inaequalis - prouzrokovača čađave krastavosti jabuke" in Institut za zaštitu bilja i životnu sredinu (2017).

TY  - JOUR
AU  - Zivkovic, Svetlana
AU  - Stošić, Stefan
AU  - Stevanović, Miloš
AU  - Gašić, Katarina
AU  - Aleksic, Goran
AU  - Vučurović, Ivan
AU  - Ristić, Danijela
PY  - 2017
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/1013
AB  - Anthracnose caused by the fungus Colletotrichum orbiculare is one of the most significant diseases of Cucurbitaceae. In Serbia watermelon fruits with typical anthracnose lesions were collected during the year of 2015. Affected fruits showed sunken, dark brown to black lesions with orange conidial masses produced in black acervuli. In an attempt to identify the causal organism, small pieces of necrotic tissue were surface sterilized and placed on potato dextrose agar (PDA). Macroscopic and microscopic morphologicaly characteristics of three isolates were observed after growth on PDA for 7 days at 25 ºC under a 12 h light/dark cycle. Fungal colonies developed white, grey to black dense aerial mycelium. Conidia were hyaline, aseptate, straight and cylindrical to clavate, 9–12.5 μm × 4–5.5 μm. Fungal isolates were also characterized by sequencing of the internal transcribed spacer (ITS) rDNA region using ITS1F/ITS4 primers and β-tubuline 2 gene using T1/Bt2b primers. The nucleotide sequences were deposited in GenBank (ITS Acc. No. KT454386, KT454387 and KT454388; β-tubuline 2 gene Acc. No. KT581236, KT581237 and KT581238). BLAST analysis of ITS and β-tubuline 2 gene sequences showed that our isolates were 100% identical to other C. orbiculare in NCBI GenBank. Pathogenicity test was conducted on symptomless, detached watermelon fruits. All tested isolates caused anthracnose lesions on watermelon fruits after 10 days of incubation. Trichoderma harzianum (DSM 63059) and Gliocladium roseum (DSM 62726) were evaluated in vitro for their antagonistic potential against C. orbiculare. The results of this study identify T. harzianum and G. roseum as promising biological control agents (BCAs) for further testing against anthracnose disease on watermelon fruits.
PB  - Herbološko društvo Srbije
T2  - Zbornik Matice srpske za prirodne nauke
T1  - Colletotrichum orbiculare on watermelon - identification and in vitro inhibition by antagonistic fungi
EP  - 343
SP  - 331
VL  - 133
DO  - 10.2298/ZMSPN1733331Z
ER  - 

@article{
author = "Zivkovic, Svetlana and Stošić, Stefan and Stevanović, Miloš and Gašić, Katarina and Aleksic, Goran and Vučurović, Ivan and Ristić, Danijela",
year = "2017",
abstract = "Anthracnose caused by the fungus Colletotrichum orbiculare is one of the most significant diseases of Cucurbitaceae. In Serbia watermelon fruits with typical anthracnose lesions were collected during the year of 2015. Affected fruits showed sunken, dark brown to black lesions with orange conidial masses produced in black acervuli. In an attempt to identify the causal organism, small pieces of necrotic tissue were surface sterilized and placed on potato dextrose agar (PDA). Macroscopic and microscopic morphologicaly characteristics of three isolates were observed after growth on PDA for 7 days at 25 ºC under a 12 h light/dark cycle. Fungal colonies developed white, grey to black dense aerial mycelium. Conidia were hyaline, aseptate, straight and cylindrical to clavate, 9–12.5 μm × 4–5.5 μm. Fungal isolates were also characterized by sequencing of the internal transcribed spacer (ITS) rDNA region using ITS1F/ITS4 primers and β-tubuline 2 gene using T1/Bt2b primers. The nucleotide sequences were deposited in GenBank (ITS Acc. No. KT454386, KT454387 and KT454388; β-tubuline 2 gene Acc. No. KT581236, KT581237 and KT581238). BLAST analysis of ITS and β-tubuline 2 gene sequences showed that our isolates were 100% identical to other C. orbiculare in NCBI GenBank. Pathogenicity test was conducted on symptomless, detached watermelon fruits. All tested isolates caused anthracnose lesions on watermelon fruits after 10 days of incubation. Trichoderma harzianum (DSM 63059) and Gliocladium roseum (DSM 62726) were evaluated in vitro for their antagonistic potential against C. orbiculare. The results of this study identify T. harzianum and G. roseum as promising biological control agents (BCAs) for further testing against anthracnose disease on watermelon fruits.",
publisher = "Herbološko društvo Srbije",
journal = "Zbornik Matice srpske za prirodne nauke",
title = "Colletotrichum orbiculare on watermelon - identification and in vitro inhibition by antagonistic fungi",
pages = "343-331",
volume = "133",
doi = "10.2298/ZMSPN1733331Z"
}

Zivkovic, S., Stošić, S., Stevanović, M., Gašić, K., Aleksic, G., Vučurović, I.,& Ristić, D.. (2017). Colletotrichum orbiculare on watermelon - identification and in vitro inhibition by antagonistic fungi. in Zbornik Matice srpske za prirodne nauke
Herbološko društvo Srbije., 133, 331-343.
https://doi.org/10.2298/ZMSPN1733331Z

Zivkovic S, Stošić S, Stevanović M, Gašić K, Aleksic G, Vučurović I, Ristić D. Colletotrichum orbiculare on watermelon - identification and in vitro inhibition by antagonistic fungi. in Zbornik Matice srpske za prirodne nauke. 2017;133:331-343.
doi:10.2298/ZMSPN1733331Z .

Zivkovic, Svetlana, Stošić, Stefan, Stevanović, Miloš, Gašić, Katarina, Aleksic, Goran, Vučurović, Ivan, Ristić, Danijela, "Colletotrichum orbiculare on watermelon - identification and in vitro inhibition by antagonistic fungi" in Zbornik Matice srpske za prirodne nauke, 133 (2017):331-343,
https://doi.org/10.2298/ZMSPN1733331Z . .

TY  - JOUR
AU  - Gavrilović, Veljko
AU  - Milićević, Zoran
AU  - Aleksić, Goran
AU  - Starović, Mira
AU  - Živković, Svetlana
AU  - Stošić, Stefan
AU  - Kuzmanović, Slobodan
PY  - 2017
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/495
AB  - The effects of copper citrate on defoliation of one year old fruit stocks in a nursery are presented in this paper. The investigation aimed to evaluate the defoliation efficacy of copper citrate as a new formulation, compared with other compounds recommended for that purpose. Field trials were conducted in the region of Trstenik, a center of nursery production in Serbia in 2016 and 2017. Two years of investigation showed that copper citrate could be used as an efficient compound for defoliation in nursery production. A stronger defoliation effect was observed on plum stocks, compared to apple stocks. Better results were achieved with the highest concentration of copper citrate (2%), while the effectiveness decreased with lower concentrations (0.5%, 1.0% and 1.5%). Defoliation degree on nursery apple stocks after treatment with 2.0% concentration of copper citrate varied from 80.6% to 95.6%, while it reached 100% on plum stocks.
AB  - U radu su prikazani efekti defolijacije sadnica jabuke i šljive, primenom različitih koncentarcija bakar citrata, kao nove formulacije, i standardnih preparata koji se koriste u ove svrhe (bordovska čorba, urea). Ogledi su sprovedeni u regionu Trstenika (Ljubava i Milutovac), koji je centar rasadničke proizvodnje u Srbiji. Tretiranja su obavljena tokom 2016. i 2017. godine. Na osnovu dobijenih rezultata zaključeno je da bakar citrat pokazuje visok potencijal za upotrebu u svrhu defolijacije sadnica jabuke i šljive. Korišćene koncentracije ovog jedinjenja su pokazale veću efikasnost u primeni na sadnicama šljive nego na sadnicama jabuke. Najbolji rezultati u oba ogleda su zabeleženi sa maksimalno primenjenom koncentracijom (2%), dok efekat defolijacije na sadnicama jabuke opada sa smanjenjem koncentracije. Stepen defolijacije jabuke primenom bakar citrata u koncentraciji od 2% kretao se od 80,6% do 95,6%, a kod šljive čak 100%. Potvrđeno je i da uspešna defolijacija sadnica jabuke i šljive značajno zavisi od vremena aplikacije kao i meteoroloških uslova.
PB  - Institute of Pesticides and Environmental Protection
T2  - Pesticidi i fitomedicina
T1  - Effects of copper citrate as a defoliant in nursery fruit stock production
T1  - Bakar citrat - defolijant u proizvodnji sadnica voćaka
EP  - 236
IS  - 3-4
SP  - 231
VL  - 32
DO  - 10.2298/PIF1704231G
ER  - 

@article{
author = "Gavrilović, Veljko and Milićević, Zoran and Aleksić, Goran and Starović, Mira and Živković, Svetlana and Stošić, Stefan and Kuzmanović, Slobodan",
year = "2017",
abstract = "The effects of copper citrate on defoliation of one year old fruit stocks in a nursery are presented in this paper. The investigation aimed to evaluate the defoliation efficacy of copper citrate as a new formulation, compared with other compounds recommended for that purpose. Field trials were conducted in the region of Trstenik, a center of nursery production in Serbia in 2016 and 2017. Two years of investigation showed that copper citrate could be used as an efficient compound for defoliation in nursery production. A stronger defoliation effect was observed on plum stocks, compared to apple stocks. Better results were achieved with the highest concentration of copper citrate (2%), while the effectiveness decreased with lower concentrations (0.5%, 1.0% and 1.5%). Defoliation degree on nursery apple stocks after treatment with 2.0% concentration of copper citrate varied from 80.6% to 95.6%, while it reached 100% on plum stocks., U radu su prikazani efekti defolijacije sadnica jabuke i šljive, primenom različitih koncentarcija bakar citrata, kao nove formulacije, i standardnih preparata koji se koriste u ove svrhe (bordovska čorba, urea). Ogledi su sprovedeni u regionu Trstenika (Ljubava i Milutovac), koji je centar rasadničke proizvodnje u Srbiji. Tretiranja su obavljena tokom 2016. i 2017. godine. Na osnovu dobijenih rezultata zaključeno je da bakar citrat pokazuje visok potencijal za upotrebu u svrhu defolijacije sadnica jabuke i šljive. Korišćene koncentracije ovog jedinjenja su pokazale veću efikasnost u primeni na sadnicama šljive nego na sadnicama jabuke. Najbolji rezultati u oba ogleda su zabeleženi sa maksimalno primenjenom koncentracijom (2%), dok efekat defolijacije na sadnicama jabuke opada sa smanjenjem koncentracije. Stepen defolijacije jabuke primenom bakar citrata u koncentraciji od 2% kretao se od 80,6% do 95,6%, a kod šljive čak 100%. Potvrđeno je i da uspešna defolijacija sadnica jabuke i šljive značajno zavisi od vremena aplikacije kao i meteoroloških uslova.",
publisher = "Institute of Pesticides and Environmental Protection",
journal = "Pesticidi i fitomedicina",
title = "Effects of copper citrate as a defoliant in nursery fruit stock production, Bakar citrat - defolijant u proizvodnji sadnica voćaka",
pages = "236-231",
number = "3-4",
volume = "32",
doi = "10.2298/PIF1704231G"
}

Gavrilović, V., Milićević, Z., Aleksić, G., Starović, M., Živković, S., Stošić, S.,& Kuzmanović, S.. (2017). Effects of copper citrate as a defoliant in nursery fruit stock production. in Pesticidi i fitomedicina
Institute of Pesticides and Environmental Protection., 32(3-4), 231-236.
https://doi.org/10.2298/PIF1704231G

Gavrilović V, Milićević Z, Aleksić G, Starović M, Živković S, Stošić S, Kuzmanović S. Effects of copper citrate as a defoliant in nursery fruit stock production. in Pesticidi i fitomedicina. 2017;32(3-4):231-236.
doi:10.2298/PIF1704231G .

Gavrilović, Veljko, Milićević, Zoran, Aleksić, Goran, Starović, Mira, Živković, Svetlana, Stošić, Stefan, Kuzmanović, Slobodan, "Effects of copper citrate as a defoliant in nursery fruit stock production" in Pesticidi i fitomedicina, 32, no. 3-4 (2017):231-236,
https://doi.org/10.2298/PIF1704231G . .